Gene:
Gene Symbol:
DIAPH3
Gene Name:
diaphanous-related formin 3
Original Clone ID:
ccsbBroadEn_04249
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC048963.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00508188
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAGTGAGGAGAGGAGCCTTTCCTTATTGGCCAAAGCCGTGGATCCCAGACACCCCAAT
ATGATGACAGATGTGGTTAAACTTCTCTCTGCGGTATGCATTGTAGGGGAAGAAAGCATC
CTTGAAGAAGTTTTAGAAGCTTTAACTTCAGCTGGTGAAGAAAAAAAAATTGACAGATTT
TTTTGTATTGTGGAAGGCCTCCGGCACAATTCAGTTCAACTGCAAGTAGCTTGTATGCAG
CTCATCAATGCCCTGGTTACATCTCCTGATGATTTGGATTTCAGGCTTCACATCAGAAAT
GAATTTATGCGTTGTGGATTGAAAGAGATATTGCCAAATTTAAAATGCATTAAGAATGAT
GGCCTGGATATCCAACTTAAAGTCTTTGATGAGCATAAAGAAGAAGATTTGTTTGAGTTA
TCCCATCGCCTTGAAGATATTAGAGCTGAACTTGATGAAGCATATGATGTTTACAACATG
GTGTGGAGCACAGTTAAAGAAACTAGAGCAGAGGGATATTTTATTTCTATTCTTCAGCAT
CTTTTGCTGATTCGAAATGATTATTTTATAAGGCAACAATACTTCAAATTAATTGATGAG
TGTGTATCCCAGATTGTATTGCATAGAGATGGAATGGATCCAGACTTCACATATCGAAAA
AGACTAGATTTAGATTTAACCCAGTTTGTAGACATTTGCATAGATCAAGCAAAACTAGAA
GAGTTTGAAGAGAAAGCATCAGAACTTTACAAGAAATTTGAAAAAGAGTTTACCGACCAC
CAAGAAACTCAGGCTGAATTGCAGAAAAAAGAGGCAAAGATTAATGAGCTTCAAGCAGAG
CTACAAGCTTTTAAGTCTCAGTTTGGTGCCTTGCCAGCTGATTGTAATATTCCTTTGCCT
CCCTCTAAAGAAGGTGGAACTGGCCACTCAGCACTTCCTCCTCCGCCTCCACTGCCTTCT
GGTGGAGGGGTGCCGCCTCCACCTCCTCCCCCACCACCTCCTCCACTTCCAGGAATGCGG
ATGCCATTCAGTGGTCCTGTGCCTCCACCACCTCCCCTGGGATTCCTTGGAGGACAAAAT
TCTCCTCCTCTACCAATCCTGCCATTTGGGTTGAAACCAAAGAAAGAATTTAAACCTGAA
ATCAGCATGAGAAGATTGAATTGGTTAAAGATCAGACCTCATGAAATGACTGAAAACTGT
TTCTGGATAAAAGTAAATGAAAATAAGTATGAAAACGTGGATTTGCTTTGTAAACTTGAG
AATACATTTTGTTGCCAACAAAAAGAGAGAAGAGAAGAGGAAGATATTGAAGAGAAGAAA
TCGATTAAGAAAAAAATTAAAGAACTTAAGTTTTTAGATTCTAAAATTGCCCAGAACCTT
TCAATCTTCCTGAGCTCTTTTCGGGTGCCATATGAGGAAATCAGAATGATGATATTGGAA
GTAGATGAAACACGGTTGGCAGAGTCTATGATTCAGAACTTAATAAAGCATCTTCCTGAT
CAAGAGCAATTAAATTCATTGTCTCAGTTCAAGAGTGAATATAGCAACTTATGTGAACCT
GAGCAGTTTGTGGTTGTGATGAGCAATGTGAAGAGACTACGGCCACGGCTCAGTGCTATT
CTCTTTAAGCTTCAGTTTGAAGAGCAGGTGAACAACATCAAACCTGACATCATGGCTGTC
AGTACTGCCTGCGAAGAGATAAAGAAGAGCAAAAGCTTTAGCAAGTTGCTGGAACTTGTA
TTGCTAATGGGAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGATTTAAC
CTTAGCTCTCTCTGTAAACTAAAGGACACAAAATCAGCAGATCAGAAAACAACGCTACTT
CATTTCCTGGTAGAAATATGTGAAGAGAAGTACCCTGATATACTGAATTTTGTGGATGAT
TTGGAACCTTTAGACAAAGCTAGTAAAGTCTCTGTAGAAACGCTGGAAAAGAATTTGAGG
CAGATGGGAAGGCAGCTTCAACAGCTTGAGAAGGAATTGGAAACCTTTCCCCCTCCTGAG
GACTTGCATGACAAGTTTGTGACAAAGATGTCCAGATTTGTTATCAGTGCAAAAGAACAA
TATGAGACACTTTCGAAGTTACACGAAAACATGGAAAAGTTATACCAGAGTATAATAGGA
TACTATGCCATTGATGTGAAGAAGGTGTCTGTGGAAGACTTTCTTACTGACCTGAATAAC
TTCAGAACCACATTCATGCAAGCAATAAAGGAGAATATCAAAAAAAGAGAAGCAGAGGAA
AAAGAAAAACGTGTCAGAATAGCTAAAGAATTAGCAGAGCGAGAAAGACTCGAACGCCAA
CAAAAGAAAAAGCGTTTATTAGAAATGAAGACTGAGGGTGATGAGACAGGAGTGATGGAT
AATCTGCTGGAGGCCTTGCAGTCCGGGGCTGCCTTCCGCGACAGAAGAAAAAGGACACCG
ATGCCAAAAGATGTTCGGCAGAGTCTCAGTCCAATGTCTCAGAGGCCTGTTCTGAAAGTT
TGTAACCATGGTAATAAACCGTATTTA
Protein sequence predicted based on nucleotide sequence
MSEERSLSLLAKAVDPRHPNMMTDVVKLLSAVCIVGEESILEEVLEALTSAGEEKKIDRF FCIVEGLRHNSVQLQVACMQLINALVTSPDDLDFRLHIRNEFMRCGLKEILPNLKCIKND GLDIQLKVFDEHKEEDLFELSHRLEDIRAELDEAYDVYNMVWSTVKETRAEGYFISILQH LLLIRNDYFIRQQYFKLIDECVSQIVLHRDGMDPDFTYRKRLDLDLTQFVDICIDQAKLE EFEEKASELYKKFEKEFTDHQETQAELQKKEAKINELQAELQAFKSQFGALPADCNIPLP PSKEGGTGHSALPPPPPLPSGGGVPPPPPPPPPPPLPGMRMPFSGPVPPPPPLGFLGGQN SPPLPILPFGLKPKKEFKPEISMRRLNWLKIRPHEMTENCFWIKVNENKYENVDLLCKLE NTFCCQQKERREEEDIEEKKSIKKKIKELKFLDSKIAQNLSIFLSSFRVPYEEIRMMILE VDETRLAESMIQNLIKHLPDQEQLNSLSQFKSEYSNLCEPEQFVVVMSNVKRLRPRLSAI LFKLQFEEQVNNIKPDIMAVSTACEEIKKSKSFSKLLELVLLMGNYMNAGSRNAQTFGFN LSSLCKLKDTKSADQKTTLLHFLVEICEEKYPDILNFVDDLEPLDKASKVSVETLEKNLR QMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQYETLSKLHENMEKLYQSIIG YYAIDVKKVSVEDFLTDLNNFRTTFMQAIKENIKKREAEEKEKRVRIAKELAERERLERQ QKKKRLLEMKTEGDETGVMDNLLEALQSGAAFRDRRKRTPMPKDVRQSLSPMSQRPVLKV CNHGNKPYL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
