Gene:
Gene Symbol:
HERC4
Gene Name:
HECT and RLD domain containing E3 ubiquitin protein ligase 4
Original Clone ID:
ccsbBroadEn_02913
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC039600.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00508194
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTTGTGCTGGGGAAATGCATCCTTTGGGCAGCTAGGTTTGGGTGGAATTGATGAAGAA
ATTGTACTAGAGCCCAGAAAAAGTGACTTCTTTATAAATAAAAGGGTCCGAGATGTAGGA
TGTGGACTCAGACATACTGTGTTTGTTCTGGATGATGGAACAGTGTACACATGTGGATGT
AATGATCTAGGACAGCTAGGTCATGAAAAATCCAGAAAGAAACCAGAGCAGGTTGTTGCC
CTGGATGCCCAAAATATTGTAGCTGTTTCATGTGGAGAAGCTCATACGTTAGCGCTAAAT
GACAAAGGCCAGGTGTATGCTTGGGGTCTCGATTCTGATGGACAGCTTGGCCTGGTAGGA
TCAGAGGAATGCATCAGAGTACCCAGAAATATTAAAAGTTTGTCAGATATCCAGATTGTA
CAGGTTGCTTGTGGTTACTATCATTCACTTGCACTTTCTAAAGCAAGTGAAGTCTTCTGT
TGGGGACAGAATAAATATGGCCAATTGGGTTTAGGTACTGACTGTAAAAAGCAAACTTCA
CCGCAGCTGCTTAAGTCTTTGCTTGGAATCCCTTTCATGCAAGTTGCAGCAGGAGGAGCC
CATAGTTTTGTACTCACCCTTTCTGGAGCTATCTTTGGATGGGGACGCAACAAGTTTGGT
CAGCTAGGTCTTAATGATGAAAATGATAGGTATGTTCCTAATTTACTAAAGTCACTAAGA
TCTCAGAAAATAGTTTATATTTGTTGTGGAGAAGATCATACTGCTGCTCTAACCAAGGAA
GGTGGAGTGTTTACTTTTGGAGCTGGAGGGTATGGTCAGTTGGGCCATAATTCTACCAGT
CATGAAATAAACCCAAGGAAAGTTTTTGAACTTATGGGAAGCATTGTCACTGAGATTGCT
TGTGGACGGCAGCACACTTCTGCTTTTGTTCCTTCATCAGGACGAATTTACTCTTTTGGG
CTTGGTGGTAATGGGCAGCTGGGAACCGGTTCAACAAGCAACAGGAAAAGCCCCTTTACT
GTAAAAGGAAATTGGTACCCCTATAATGGGCAGTGTCTACCAGATATTGATTCTGAAGAA
TATTTCTGTGTAAAAAGAATTTTCTCAGGGGGAGATCAAAGCTTTTCACATTACTCTAGT
CCCCAGAACTGTGGGCCACCAGATGACTTCAGATGTCCCAATCCGACAAAGCAGATCTGG
ACAGTGAATGAAGCTCTAATTCAGAAATGGCTGAGCTATCCTTCTGGAAGGTTTCCTGTG
GAGATAGCCAATGAGATAGATGGAACGTTTTCTTCCTCTGGTTGCCTAAATGGAAGTTTT
TTAGCTGTTAGCAATGATGATCACTATAGAACAGGTACCAGATTTTCAGGGGTTGATATG
AATGCTGCTAGGCTTTTATTCCACAAACTTATACAACCTGATCATCCGCAGATATCTCAG
CAGGTGGCAGCTAGTTTGGAAAAGAATCTTATTCCTAAACTGACTAGCTCCTTACCTGAT
GTTGAAGCATTGAGGTTTTATCTTACTCTACCAGAATGTCCCCTGATGAGTGATTCCAAC
AATTTCACAACAATAGCAATTCCCTTTGGTACAGCTCTTGTGAACCTAGAAAAGGCACCA
CTGAAAGTACTTGAAAACTGGTGGTCAGTACTTGAACCTCCACTATTCCTCAAGATAGTA
GAACTTTTTAAGGAAGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGTATTCCCCCT
TCTGAAAGAAGAATTTTCAACAGTTTTCTTCATACTGCATTAAAGGTTTTAGAAATACTA
CATAGGGTAAATGAGAAAATGGGACAGATTATACAGTATGATAAATTTTATATACATGAA
GTACAAGAATTGATAGACATAAGAAATGATTATATCAACTGGGTCCAACAGCAGGCCTAT
GGAATGTTGGCAGATATCCCTGTTACAATCTGTACATATCCATTTGTATTTGATGCCCAA
GCAAAAACTACTCTGTTACAGACCGATGCAGTCTTACAGATGCAGATGGCTATTGATCAG
GCCCACAGGCAGAATGTCTCCTCTCTTTTTCTCCCAGTGATTGAATCTGTGAATCCCTGC
TTAATTCTAGTGGTGCGTAGAGAAAATATTGTAGGAGATGCAATGGAAGTCCTTAGGAAA
ACAAAGAACATAGATTACAAGAAGCCACTCAAGGTTATATTTGTTGGAGAAGATGCTGTG
GATGCAGGAGGGGTGCGCAAAGAATTTTTCTTGCTCATCATGAGGGAATTATTGGATCCT
AAATACGGCATGTTTAGGTATTATGAAGATTCCAGGCTCATTTGGTTTTCTGATAAGACA
TTTGAAGACAGTGATTTGTTCCATTTGATTGGTGTTATCTGTGGCTTAGCAATTTATAAT
TGTACCATTGTGGACCTCCATTTTCCTTTGGCTTTATATAAGAAACTACTGAAAAAGAAG
CCATCCTTGGATGATTTGAAAGAACTAATGCCTGATGTTGGGAGAAGCATGCAACAGTTA
CTGGATTATCCAGAAGATGACATAGAGGAAACATTTTGTCTTAATTTTACGATCACAGTT
GAAAACTTTGGTGCAACAGAAGTGAAAGAGCTGGTTCTAAATGGTGCAGACACAGCTGTT
AACAAACAAAATCGGCAAGAGTTTGTCGATGCTTATGTGGATTACATATTCAATAAATCA
GTGGCTTCCTTATTTGATGCTTTTCATGCGGGCTTTCATAAGGTCTGTGGAGGAAAAGTC
CTTCTGCTCTTTCAGCCTAATGAACTACAAGCAATGGTCATTGGAAATACAAATTATGAT
TGGAAGGAACTGGAAAAGAATACAGAATACAAAGGGGAATATTGGGCAGAACATCCTACG
ATAAAAATTTTTTGGGAAGTATTTCACGAATTACCATTGGAAAAGAAGAAACAGTTTCTG
TTATTTTTGACAGGTAGTGATCGCATTCCTATTCTTGGTATGAAGAGTCTGAAACTAGTC
ATCCAGTCCACAGGAGGTGGTGAGGAGTATCTCCCAGTTTCCCATACTTGTTTTAATCTT
CTGGATCTTCCAAAATATACAGAAAAAGAAACTCTACGCTCTAAACTGATCCAAGCTATT
GATCACAATGAAGGCTTCAGTTTAATA
Protein sequence predicted based on nucleotide sequence
MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGC NDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVG SEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTS PQLLKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLR SQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTEIA CGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNGQCLPDIDSEE YFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPV EIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQ QVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAP LKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEIL HRVNEKMGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQ AKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRK TKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKT FEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPDVGRSMQQL LDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKS VASLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPT IKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNL LDLPKYTEKETLRSKLIQAIDHNEGFSLI
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
