Gene:
Gene Symbol:
RIMS2
Gene Name:
regulating synaptic membrane exocytosis 2
Original Clone ID:
ccsbBroadEn_07470
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC043144.2
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00508197
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCAATTTGAGACATTGCGCCAGGTCTGCAATTCTGTTTTATCTCATTTTCATGGGGTT
TTTTCATCCCCACCAAATATCTTACAAAATGAGCTTTTTGGACAAACACTGAACAATGCA
AGGAAAAGAAGCCCATCTGTGTCCAGAGATCAGAATAGAAGATACGACCAAAGGGAAGAA
AGAGAGGAATATTCACAGTATGCTACTTCGGATACCGCAATGCCTAGATCTCCATCAGAT
TATGCTGATAGGCGATTTCAACATGAACCTCAGTTTTATGAAGACTCTGATCATTTAAGT
TATAGGGACTCCAACAGGAGAAGTCATAGGCATTCCAAAGAATATATTGTAGATGATGAG
GATGTGGAAAGCAGAGATGAATACGAAAGGCAAAGGAGAGAGGAAGAGTACCAGTCACGC
TACCGAAGTGATCCGAATTTGGCCCGTTATCCAGTAAAGCCACAACCCTATGAAGAACAA
ATGCGGATCCATGCTGAAGTGTCCCGAGCACGGCATGAGAGAAGGCATAGTGATGTTTCT
TTGGCAAATGCTGATCTGGAAGATTCCAGGATTTCTATGCTAAGGATGGATCGACCATCA
AGGCAAAGATCTATATCAGAACGTAGAGCTGCCATGGAAAATCAGCGATCTTATTCAATG
GAAAGAACTCGAGAGGCTCAGGGACCAAGTTCTTATGCACAAAGGACCACAAACCATAGT
CCTCCTACCCCCAGGAGGAGTCCACTACCCATAGATAGACCAGACTTGAGGCGTACTGAC
TCACTACGGAAACAGCACCACTTAGATCCTAGCTCTGCTGTAAGAAAAACAAAACGGGAA
AAAATGGAAACAATGTTAAGGAATGATTCTCTCAGTTCAGACCGGTCAGAGTCAGTGAGA
CCTCCACCACCAAAGCCTCATAAATCAAAGAAAGGCGGTAAAATGCGCCAGATTTCGTTG
AGCAGTTCAGAGGAGGAATTGGCTTCCACGCCTGAATATACAAGTTGTGATGATGTTGAG
ATTGAAAGTGAGAGTGTAAGTGAAAAAGGAGACATGGATTACAACTGGTTGGATCATACG
TCTTGGCATAGCAGTGAGGCATCCCCAATGTCTTTGCACCCTGTAACCTGGCAACCATCT
AAAGATGGAGATCGTTTAATTGGTCGCATTTTATTAAATAAGCGTCTAAAAGATGGAAGT
GTACCTCGAGATTCAGGAGCAATGCTTGGCTTGAAGGTTGTAGGAGGAAAGATGACTGAA
TCAGGTCGGCTTTGTGCATTTATTACTAAAGTAAAAAAAGGAAGTTTAGCTGATACTGTA
GGACATCTTAGACCAGGTGATGAAGTATTAGAATGGAATGGAAGACTACTGCAAGGAGCC
ACATTTGAGGAAGTGTACAACATCATTCTAGAATCCAAACCTGAACCACAAGTAGAACTT
GTAGTTTCAAGGCCTATTGGAGATATACCGCGAATACCTGATAGCACACATGCACAACTG
GAGTCCAGTTCTAGCTCCTTTGAATCTCAAAAAATGGATCGTCCTTCTATTTCTGTTACC
TCTCCCATGAGTCCTGGAATGTTGAGGGATGTCCCACAGTTCTTATCAGGACAACTTTCA
ATAAAACTATGGTTTGACAAGGTTGGTCACCAATTAATAGTTACAATTTTGGGAGCAAAA
GATCTCCCTTCCAGGGAAGATGGGAGGCCAAGGAATCCTTATGTTAAAATTTACTTTCTT
CCAGACAGAAGTGATAAAAACAAGAGAAGAACTAAAACAGTAAAGAAAACATTGGAACCC
AAATGGAACCAAACATTCATTTATTCTCCAGTCCACCGAAGAGAATTTCGGGAACGAATG
CTAGAGATTACCCTTTGGGATCAAGCTCGTGTTCGAGAGGAAGAAAGTGAATTCTTAGGG
GAGATTTTAATTGAATTAGAAACAGCATTATTAGATGATGAGCCACATTGGTACAAACTT
CAGACGCATGATGTCTCTTCATTGCCACTTCCCCACCCTTCTCCATATATGCCACGAAGA
CAGCTCCATGGAGAGAGCCCAACACGGAGGTTGCAAAGGTCAAAGAGAATAAGTGATAGT
GAAGTCTCTGACTATGACTGTGATGATGGAATTGGTGTAGTATCAGATTATCGACATGAT
GGTCGAGATCTTCAAAGCTCAACATTATCAGTGCCAGAACAAGTAATGTCATCAAACCAC
TGTTCACCATCAGGGTCTCCTCATCGAGTAGATGTTATAGGAAGGACTAGATCATGGTCA
CCCAGTGTCCCTCCTCCACAAAGTCGGAATGTGGAACAGGGGCTTCGAGGGACCCGCACT
ATGACCGGACATTATAATACAATTAGCCGAATGGACAGACATCGTGTCATGGATGACCAT
TATTCTCCAGATAGAGACAGTCATTTTCTTACTCTACCTCGCTCCAGATACAGTCAGACC
ATTGACCATCATCACAGGGATGGCAGGGATTGTGAAGCAGCAGATAGACAGCCATATCAC
AGATCCAGATCAACAGAACAACGGCCTCTCCTTGAGCGGACCACCACCCGCTCCAGATCC
ACTGAACGTCCTGATACAAACCTCATGAGGTCGATGCCTTCATTAATGACTGGAAGATCT
GCCCCTCCTTCACCTGCCTTATCGAGGTCTCATCCTCGTACTGGGTCTGTCCAGACAAGC
CCATCAAGTACTCCAGTCGCAGGACGAAGGGGCCGACAGCTTCCACAGCTTCCACCAAAG
GGAACGTTGGATAGAAAAGCAGGAGGTAAAAAACTAAGGAGCACTGTCCAAAGAAGTACA
GAAACAGGCCTGGCCGTGGAAATGAGGAACTGGATGACTCGACAGGCAAGCCGAGAGTCT
ACAGATGGTAGCATGAACAGCTACAGCTCAGAAGGAAATCTGATTTTCCCTGGTGTTCGC
TTGGCCTCTGATAGCCAGTTCAGTGATTTCCTGGATGGCCTTGGCCCTGCTCAGCTAGTG
GGACGCCAGACTCTGGCAACACCTGCAATGGGTGACATTCAGGTAGGAATGATGGACAAA
AAGGGACAGCTGGAGGTAGAAATCATCCGGGCCCGTGGCCTTGTTGTAAAACCAGGTTCC
AAGACACTGCCAGCACCGTATGTAAAAGTGTATCTATTAGATAACGGAGTCTGCATAGCC
AAAAAGAAAACAAAAGTGGCAAGAAAAACGCTGGAACCCCTTTACCAGCAGCTATTATCT
TTCGAAGAGAGTCCACAAGGAAAAGTTTTACAGATCATCGTCTGGGGAGATTATGGCCGC
ATGGATCACAAATCTTTTATGGGAGTGGCCCAGATACTTTTAGATGAACTAGAGCTATCC
AATATGGTGATCGGATGGTTCAAACTTTTCCCACCTTCCTCCCTAGTAGATCCAACCTTG
GCCCCTCTGACAAGAAGAGCTTCCCAATCATCTCTGGAAAGTTCAACTGGACCTTCTTAC
TCTCGTTCA
Protein sequence predicted based on nucleotide sequence
MQFETLRQVCNSVLSHFHGVFSSPPNILQNELFGQTLNNARKRSPSVSRDQNRRYDQREE REEYSQYATSDTAMPRSPSDYADRRFQHEPQFYEDSDHLSYRDSNRRSHRHSKEYIVDDE DVESRDEYERQRREEEYQSRYRSDPNLARYPVKPQPYEEQMRIHAEVSRARHERRHSDVS LANADLEDSRISMLRMDRPSRQRSISERRAAMENQRSYSMERTREAQGPSSYAQRTTNHS PPTPRRSPLPIDRPDLRRTDSLRKQHHLDPSSAVRKTKREKMETMLRNDSLSSDRSESVR PPPPKPHKSKKGGKMRQISLSSSEEELASTPEYTSCDDVEIESESVSEKGDMDYNWLDHT SWHSSEASPMSLHPVTWQPSKDGDRLIGRILLNKRLKDGSVPRDSGAMLGLKVVGGKMTE SGRLCAFITKVKKGSLADTVGHLRPGDEVLEWNGRLLQGATFEEVYNIILESKPEPQVEL VVSRPIGDIPRIPDSTHAQLESSSSSFESQKMDRPSISVTSPMSPGMLRDVPQFLSGQLS IKLWFDKVGHQLIVTILGAKDLPSREDGRPRNPYVKIYFLPDRSDKNKRRTKTVKKTLEP KWNQTFIYSPVHRREFRERMLEITLWDQARVREEESEFLGEILIELETALLDDEPHWYKL QTHDVSSLPLPHPSPYMPRRQLHGESPTRRLQRSKRISDSEVSDYDCDDGIGVVSDYRHD GRDLQSSTLSVPEQVMSSNHCSPSGSPHRVDVIGRTRSWSPSVPPPQSRNVEQGLRGTRT MTGHYNTISRMDRHRVMDDHYSPDRDSHFLTLPRSRYSQTIDHHHRDGRDCEAADRQPYH RSRSTEQRPLLERTTTRSRSTERPDTNLMRSMPSLMTGRSAPPSPALSRSHPRTGSVQTS PSSTPVAGRRGRQLPQLPPKGTLDRKAGGKKLRSTVQRSTETGLAVEMRNWMTRQASRES TDGSMNSYSSEGNLIFPGVRLASDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQVGMMDK KGQLEVEIIRARGLVVKPGSKTLPAPYVKVYLLDNGVCIAKKKTKVARKTLEPLYQQLLS FEESPQGKVLQIIVWGDYGRMDHKSFMGVAQILLDELELSNMVIGWFKLFPPSSLVDPTL APLTRRASQSSLESSTGPSYSRS
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
