DNASU Plasmid | INO80C (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: INO80C

Gene Name: INO80 complex subunit C

Original Clone ID:

ccsbBroadEn_13113

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC048989.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00508282

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 412nts

Open reading frame: 1 to 412

Reference Sequence Alignment

ATGGAAGCCATGAGTGAGAATAAAATGGTGCCCTCTGAGTTTAGCACAGGACCTGTGGAA
AAAGCTGCCAAACCTTTGCCATTTAAGGATCCCAACTTTGTGCACTCTGGCCACGGTGGC
GCAGTAGCTGGCAAGAAGAACAGAACCTGGAAGAACCTGAAACAAATCCTCGCTTCTGAA
AGGGCATTGCCGTGGCAACTGAACGATCCTAACTACTTCAGTATTGATGCTCCTCCATCC
TTTAAGCCAGCTAAGAAGTATTCTGATGTTTCAGGTCTGCTTGCCAACTACACAGACCCC
CAGAGCAAACTGCGGTTCAGCACCATTGAAGAGTTTTCCTACATTCGGAGGCTGCCCTCT
GACGTCGTCACCGGCTACCTGGCCCTGAGGAAGGCCACGAGCATCGTTCCC

Protein sequence predicted based on nucleotide sequence

MEAMSENKMVPSEFSTGPVEKAAKPLPFKDPNFVHSGHGGAVAGKKNRTWKNLKQILASE
RALPWQLNDPNYFSIDAPPSFKPAKKYSDVSGLLANYTDPQSKLRFSTIEEFSYIRRLPS
DVVTGYLALRKATSIVP

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

INO80C

Symbol Nomenclature :

INO80C

SYNONYM :

C18orf37; IES6; hIes6

Designation :

IES6 homolog

Full Nomenclature :

INO80 complex subunit C

GENEID :

125476

HGNC :

26994

Vega :

OTTHUMG00000132564

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00508282        ------------------------------------------------------------
NM_194281.3         ATGGCGGCGCAAATTCCAATTGTGGCCACCACTTCCACTCCCGGAATAGTCCGGAACAGC
NM_001098817.1      ATGGCGGCGCAAATTCCAATTGTGGCCACCACTTCCACTCCCGGAATAGTCCGGAACAGC
                                                                                

HsCD00508282        ------------------------------------------------------------
NM_194281.3         AAGAAGAGGCCGGCCAGCCCTTCCCACAATGGCAGCAGCGGCGGGGGCTATGGCGCCAGT
NM_001098817.1      AAGAAGAGGCCGGCCAGCCCTTCCCACAATGGCAGCAGCGGCGGGGGCTATGGCGCCAGT
                                                                                

HsCD00508282        ------------------------------------------------------------
NM_194281.3         AAGAAGAAAAAAGCGTCCGCTTCCAGCTTTGCGCAGGG----------------------
NM_001098817.1      AAGAAGAAAAAAGCGTCCGCTTCCAGCTTTGCGCAGACGTGCCTTTCGCCTTCCACCATG
                                                                                

HsCD00508282        ------------------------------------------------------------
NM_194281.3         ------------------------------------------------------------
NM_001098817.1      ATTGTGAGGCCTCCCCAGCCACGTGGAACTACGTGCCTCTTGCCTTCCGCCATGATTGTG
                                                                                

HsCD00508282        ---------------------------------ATGGAAGCCATGAGTGAGAATAAAATG
NM_194281.3         --------------------------TATCAGCATGGAAGCCATGAGTGAGAATAAAATG
NM_001098817.1      AGGCCTCCCCAGCCGCGTGGAAATGGTATCAGCATGGAAGCCATGAGTGAGAATAAAATG
                                                     ***************************

HsCD00508282        GTGCCCTCTGAGTTTAGCACAGGACCTGTGGAAAAAGCTGCCAAACCTTTGCCATTTAAG
NM_194281.3         GTGCCCTCTGAGTTTAGCACAGGACCTGTGGAAAAAGCTGCCAAACCTTTGCCATTTAAG
NM_001098817.1      GTGCCCTCTGAGTTTAGCACAGGACCTGTGGAAAAAGCTGCCAAACCTTTGCCATTTAAG
                    ************************************************************

HsCD00508282        GATCCCAACTTTGTGCACTCTGGCCACGGTGGCGCAGTAGCTGGCAAGAAGAACAGAACC
NM_194281.3         GATCCCAACTTTGTGCACTCTGGCCACGGTGGCGCAGTAGCTGGCAAGAAGAACAGAACC
NM_001098817.1      GATCCCAACTTTGTGCACTCTGGCCACGGTGGCGCAGTAGCTGGCAAGAAGAACAGAACC
                    ************************************************************

HsCD00508282        TGGAAGAACCTGAAACAAATCCTCGCTTCTGAAAGGGCATTGCCGTGGCAACTGAACGAT
NM_194281.3         TGGAAGAACCTGAAACAAATCCTCGCTTCTGAAAGGGCATTGCCGTGGCAACTGAACGAT
NM_001098817.1      TGGAAGAACCTGAAACAAATCCTCGCTTCTGAAAGGGCATTGCCGTGGCAACTGAACGAT
                    ************************************************************

HsCD00508282        CCTAACTACTTCAGTATTGATGCTCCTCCATCCTTTAAGCCAGCTAAGAAGTATTCTGAT
NM_194281.3         CCTAACTACTTCAGTATTGATGCTCCTCCATCCTTTAAGCCAGCTAAGAAGTATTCTGAT
NM_001098817.1      CCTAACTACTTCAGTATTGATGCTCCTCCATCCTTTAAGCCAGCTAAGAAGTATTCTGAT
                    ************************************************************

HsCD00508282        GTTTCAGGTCTGCTTGCCAACTACACAGACCCCCAGAGCAAACTGCGGTTCAGCACCATT
NM_194281.3         GTTTCAGGTCTGCTTGCCAACTACACAGACCCCCAGAGCAAACTGCGGTTCAGCACCATT
NM_001098817.1      GTTTCAGGTCTGCTTGCCAACTACACAGACCCCCAGAGCAAACTGCGGTTCAGCACCATT
                    ************************************************************

HsCD00508282        GAAGAGTTTTCCTACATTCGGAGGCTGCCCTCTGACGTCGTCACCGGCTACCTGGCCCTG
NM_194281.3         GAAGAGTTTTCCTACATTCGGAGGCTGCCCTCTGACGTCGTCACCGGCTACCTGGCCCTG
NM_001098817.1      GAAGAGTTTTCCTACATTCGGAGGCTGCCCTCTGACGTCGTCACCGGCTACCTGGCCCTG
                    ************************************************************

HsCD00508282        AGGAAGGCCACGAGCATCGTTCCC---
NM_194281.3         AGGAAGGCCACGAGCATCGTTCCCTGA
NM_001098817.1      AGGAAGGCCACGAGCATCGTTCCCTGA
                    ************************