DNASU Plasmid | KLK15 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: KLK15

Gene Name: kallikrein-related peptidase 15

Original Clone ID:

ccsbBroadEn_08543

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC126137.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00508444

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 769nts

Open reading frame: 1 to 769

Reference Sequence Alignment

ATGTGGCTTCTCCTCACTCTCTCCTTCCTGCTGGCATCCACAGCAGCCCAGGATGGTGAC
AAGTTGCTGGAAGGTGACGAGTGTGCACCCCACTCCCAGCCATGGCAAGTGGCTCTCTAC
GAGCGTGGACGCTTTAACTGTGGCGCTTCCCTCATCTCCCCACACTGGGTGCTGTCTGCG
GCACACTGCCAAAGCCGCTTCATGAGAGTGCGCCTGGGAGAGCACAACCTGCGCAAGCGC
GATGGCCCAGAGCAACTACGGACCACGTCTCGGGTCATTCCACACCCGCGCTACGAAGCG
CGCAGCCACCGCAACGACATCATGTTGCTGCGCCTAGTCCAGCCCGCACGCCTGAACCCC
CAGGTGCGCCCCGCGGTGCTACCCACGCGTTGCCCCCACCCGGGGGAGGCCTGTGTGGTG
TCTGGCTGGGGCCTGGTGTCCCACAACGAGCCTGGGACCGCTGGGAGCCCCCGGTCACAA
GTGAGTCTCCCAGATACGTTGCATTGTGCCAACATCAGCATTATCTCGGACACATCTTGT
GACAAGAGCTACCCAGGGCGCCTGACAAACACCATGGTGTGTGCAGGCGCGGAGGGCAGA
GGCGCAGAATCCTGTGAGGGTGACTCTGGGGGACCCCTGGTCTGTGGGGGCATCCTGCAG
GGCATTGTGTCCTGGGGTGACGTCCCTTGTGACAACACCACCAAGCCTGGTGTCTATACC
AAAGTCTGCCACTACTTGGAGTGGATCAGGGAAACCATGAAGAGGAAC

Protein sequence predicted based on nucleotide sequence

MWLLLTLSFLLASTAAQDGDKLLEGDECAPHSQPWQVALYERGRFNCGASLISPHWVLSA
AHCQSRFMRVRLGEHNLRKRDGPEQLRTTSRVIPHPRYEARSHRNDIMLLRLVQPARLNP
QVRPAVLPTRCPHPGEACVVSGWGLVSHNEPGTAGSPRSQVSLPDTLHCANISIISDTSC
DKSYPGRLTNTMVCAGAEGRGAESCEGDSGGPLVCGGILQGIVSWGDVPCDNTTKPGVYT
KVCHYLEWIRETMKRN

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

KLK15

Symbol Nomenclature :

KLK15

SYNONYM :

ACO; HSRNASPH

Designation :

ACO protease|kallikrein-15|kallikrein-like serine protease|prostinogen

Full Nomenclature :

kallikrein-related peptidase 15

GENEID :

55554

GenBank Accession :

HQ448402

HGNC :

20453

MIM :

610601

Vega :

OTTHUMG00000183116

Target GenBank:

HQ448402

Reference Sequence Alignment

  

HsCD00508444        ATGTGGCTTCTCCTCACTCTCTCCTTCCTGCTGGCATCCACAGCAGCCCAGGATGGTGAC
NM_017509.3         ATGTGGCTTCTCCTCACTCTCTCCTTCCTGCTGGCATCCACAGCAGCCCAGGATGGTGAC
NM_001277081.1      ATGTGGCTTCTCCTCACTCTCTCCTTCCTGCTG---GCATCCACAGCCCAGGATGGTGAC
                    *********************************    *.:*..*****************

HsCD00508444        AAGTTGCTGGAAGGTGACGAGTGTGCACCCCACTCCCAGCCATGGCAAGTGGCTCTCTAC
NM_017509.3         AAGTTGCTGGAAGGTGACGAGTGTGCACCCCACTCCCAGCCATGGCAAGTGGCTCTCTAC
NM_001277081.1      AAGTTGCTGGAAGGTGACGAGTGTGCACCCCACTCCCAGCCATGGCAAGTGGCTCTCTAC
                    ************************************************************

HsCD00508444        GAGCGTGGACGCTTTAACTGTGGCGCTTCCCTCATCTCCCCACACTGGGTGCTGTCTGCG
NM_017509.3         GAGCGTGGACGCTTTAACTGTGGCGCTTCCCTCATCTCCCCACACTGGGTGCTGTCTGCG
NM_001277081.1      GAGCGTGGACGCTTTAACTGTGGCGCTTCCCTCATCTCCCCACACTGGGTGCTGTCTGCG
                    ************************************************************

HsCD00508444        GCACACTGCCAAAGCCGCTTCATGAGAGTGCGCCTGGGAGAGCACAACCTGCGCAAGCGC
NM_017509.3         GCCCACTGCCAAAGCCGCTTCATGAGAGTGCGCCTGGGAGAGCACAACCTGCGCAAGCGC
NM_001277081.1      GCCCACTGCCAAAGCCGCTTCATGAGAGTGCGCCTGGGAGAGCACAACCTGCGCAAGCGC
                    **.*********************************************************

HsCD00508444        GATGGCCCAGAGCAACTACGGACCACGTCTCGGGTCATTCCACACCCGCGCTACGAAGCG
NM_017509.3         GATGGCCCAGAGCAACTACGGACCACGTCTCGGGTCATTCCACACCCGCGCTACGAAGCG
NM_001277081.1      GATGGCCCAGAGCAACTACGGACCACGTCTCGGGTCATTCCACACCCGCGCTACGAAGCG
                    ************************************************************

HsCD00508444        CGCAGCCACCGCAACGACATCATGTTGCTGCGCCTAGTCCAGCCCGCACGCCTGAACCCC
NM_017509.3         CGCAGCCACCGCAACGACATCATGTTGCTGCGCCTAGTCCAGCCCGCACGCCTGAACCCC
NM_001277081.1      CGCAGCCACCGCAACGACATCATGTTGCTGCGCCTAGTCCAGCCCGCACGCCTGAACCCC
                    ************************************************************

HsCD00508444        CAGGTGCGCCCCGCGGTGCTACCCACGCGTTGCCCCCACCCGGGGGAGGCCTGTGTGGTG
NM_017509.3         CAGGTGCGCCCCGCGGTGCTACCCACGCGTTGCCCCCACCCGGGGGAGGCCTGTGTGGTG
NM_001277081.1      CAGGTGCGCCCCGCGGTGCTACCCACGCGTTGCCCCCACCCGGGGGAGGCCTGTGTGGTG
                    ************************************************************

HsCD00508444        TCTGGCTGGGGCCTGGTGTCCCACAACGAGCCTGGGACCGCTGGGAGCCCCCGGTCACAA
NM_017509.3         TCTGGCTGGGGCCTGGTGTCCCACAACGAGCCTGGGACCGCTGGGAGCCCCCGGTCACAA
NM_001277081.1      TCTGGCTGGGGCCTGGTGTCCCACAACGAGCCTGGGACCGCTGGGAGCCCCCGGTCACAA
                    ************************************************************

HsCD00508444        GTGAGTCTCCCAGATACGTTGCATTGTGCCAACATCAGCATTATCTCGGACACATCTTGT
NM_017509.3         GTGAGTCTCCCAGATACGTTGCATTGTGCCAACATCAGCATTATCTCGGACACATCTTGT
NM_001277081.1      GTGAGTCTCCCAGATACGTTGCATTGTGCCAACATCAGCATTATCTCGGACACATCTTGT
                    ************************************************************

HsCD00508444        GACAAGAGCTACCCAGGGCGCCTGACAAACACCATGGTGTGTGCAGGCGCGGAGGGCAGA
NM_017509.3         GACAAGAGCTACCCAGGGCGCCTGACAAACACCATGGTGTGTGCAGGCGCGGAGGGCAGA
NM_001277081.1      GACAAGAGCTACCCAGGGCGCCTGACAAACACCATGGTGTGTGCAGGCGCGGAGGGCAGA
                    ************************************************************

HsCD00508444        GGCGCAGAATCCTGTGAGGGTGACTCTGGGGGACCCCTGGTCTGTGGGGGCATCCTGCAG
NM_017509.3         GGCGCAGAATCCTGTGAGGGTGACTCTGGGGGACCCCTGGTCTGTGGGGGCATCCTGCAG
NM_001277081.1      GGCGCAGAATCCTGTGAGGGTGACTCTGGGGGACCCCTGGTCTGTGGGGGCATCCTGCAG
                    ************************************************************

HsCD00508444        GGCATTGTGTCCTGGGGTGACGTCCCTTGTGACAACACCACCAAGCCTGGTGTCTATACC
NM_017509.3         GGCATTGTGTCCTGGGGTGACGTCCCTTGTGACAACACCACCAAGCCTGGTGTCTATACC
NM_001277081.1      GGCATTGTGTCCTGGGGTGACGTCCCTTGTGACAACACCACCAAGCCTGGTGTCTATACC
                    ************************************************************

HsCD00508444        AAAGTCTGCCACTACTTGGAGTGGATCAGGGAAACCATGAAGAGGAAC---
NM_017509.3         AAAGTCTGCCACTACTTGGAGTGGATCAGGGAAACCATGAAGAGGAACTGA
NM_001277081.1      AAAGTCTGCCACTACTTGGAGTGGATCAGGGAAACCATGAAGAGGAACTGA
                    ************************************************