DNASU Plasmid | EIF3H (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: EIF3H

Gene Name: eukaryotic translation initiation factor 3, subunit H

Original Clone ID:

ccsbBroadEn_01985

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC000386.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00510316

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1057nts

Open reading frame: 1 to 1057

Reference Sequence Alignment

ATGGCGTCCCGCAAGGAAGGTACCGGCTCTACTGCCACCTCTTCCAGCTCCACCGCCGGC
GCAGCAGGGAAAGGCAAAGGCAAAGGCGGCTCGGGAGATTCAGCCGTGAAGCAAGTGCAG
ATAGATGGCCTTGTGGTATTAAAGATAATCAAACATTATCAAGAAGAAGGACAAGGAACT
GAAGTTGTTCAAGGAGTGCTTTTGGGTCTGGTTGTAGAAGATCGGCTTGAAATTACCAAC
TGCTTTCCTTTCCCTCAGCACACAGAGGATGATGCTGACTTTGATGAAGTCCAATATCAG
ATGGAAATGATGCGGAGCCTTCGCCATGTAAACATTGATCATCTTCACGTGGGCTGGTAT
CAGTCCACATACTATGGCTCATTCGTTACCCGGGCACTCCTGGACTCTCAGTTTAGTTAC
CAGCATGCCATTGAAGAATCTGTCGTTCTCATTTATGATCCCATAAAAACTGCCCAAGGA
TCTCTCTCACTAAAGGCATACAGACTGACTCCTAAACTGATGGAAGTTTGTAAAGAAAAG
GATTTTTCCCCTGAAGCATTGAAAAAAGCAAATATCACCTTTGAGTACATGTTTGAAGAA
GTGCCGATTGTAATTAAAAATTCACATCTGATCAATGTCCTAATGTGGGAACTTGAAAAG
AAGTCAGCTGTTGCAGATAAACATGAATTGCTCAGCCTTGCCAGCAGCAATCATTTGGGG
AAGAATCTACAGTTGCTGATGGACAGAGTGGATGAAATGAGCCAAGATATAGTTAAATAC
AACACATACATGAGGAATACTAGTAAACAACAGCAGCAGAAACATCAGTATCAGCAGCGT
CGCCAGCAGGAGAATATGCAGCGCCAGAGCCGAGGAGAACCCCCGCTCCCTGAGGAGGAC
CTGTCCAAACTCTTCAAACCACCACAGCCGCCTGCCAGGATGGACTCGCTGCTCATTGCA
GGCCAGATAAACACTTACTGCCAGAACATCAAGGAGTTCACTGCCCAAAACTTAGGCAAG
CTCTTCATGGCCCAGGCTCTTCAAGAATACAACAAC

Protein sequence predicted based on nucleotide sequence

MASRKEGTGSTATSSSSTAGAAGKGKGKGGSGDSAVKQVQIDGLVVLKIIKHYQEEGQGT
EVVQGVLLGLVVEDRLEITNCFPFPQHTEDDADFDEVQYQMEMMRSLRHVNIDHLHVGWY
QSTYYGSFVTRALLDSQFSYQHAIEESVVLIYDPIKTAQGSLSLKAYRLTPKLMEVCKEK
DFSPEALKKANITFEYMFEEVPIVIKNSHLINVLMWELEKKSAVADKHELLSLASSNHLG
KNLQLLMDRVDEMSQDIVKYNTYMRNTSKQQQQKHQYQQRRQQENMQRQSRGEPPLPEED
LSKLFKPPQPPARMDSLLIAGQINTYCQNIKEFTAQNLGKLFMAQALQEYNN

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

EIF3H

Symbol Nomenclature :

EIF3H

SYNONYM :

EIF3S3; eIF3-gamma; eIF3-p40

Designation :

eIF-3-gamma|eIF3 p40 subunit|eukaryotic translation initiation factor 3 subunit 3|eukaryotic translation initiation factor 3 subunit H|eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD)|eukaryotic translation initiation factor 3, subunit 3 (gamma, 40kD)|eukaryotic translation initiation factor 3, subunit 3 gamma, 40kDa

Full Nomenclature :

eukaryotic translation initiation factor 3, subunit H

GENEID :

8667

HGNC :

3273

MIM :

603912

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00510316      ATGGCGTCCCGCAAGGAAGGTACCGGCTCTACTGCCACCTCTTCCAGCTCCACCGCCGGC
NM_003756.2       ATGGCGTCCCGCAAGGAAGGTACCGGCTCTACTGCCACCTCTTCCAGCTCCACCGCCGGC
                  ************************************************************

HsCD00510316      GCAGCAGGGAAAGGCAAAGGCAAAGGCGGCTCGGGAGATTCAGCCGTGAAGCAAGTGCAG
NM_003756.2       GCAGCAGGGAAAGGCAAAGGCAAAGGCGGCTCGGGAGATTCAGCCGTGAAGCAAGTGCAG
                  ************************************************************

HsCD00510316      ATAGATGGCCTTGTGGTATTAAAGATAATCAAACATTATCAAGAAGAAGGACAAGGAACT
NM_003756.2       ATAGATGGCCTTGTGGTATTAAAGATAATCAAACATTATCAAGAAGAAGGACAAGGAACT
                  ************************************************************

HsCD00510316      GAAGTTGTTCAAGGAGTGCTTTTGGGTCTGGTTGTAGAAGATCGGCTTGAAATTACCAAC
NM_003756.2       GAAGTTGTTCAAGGAGTGCTTTTGGGTCTGGTTGTAGAAGATCGGCTTGAAATTACCAAC
                  ************************************************************

HsCD00510316      TGCTTTCCTTTCCCTCAGCACACAGAGGATGATGCTGACTTTGATGAAGTCCAATATCAG
NM_003756.2       TGCTTTCCTTTCCCTCAGCACACAGAGGATGATGCTGACTTTGATGAAGTCCAATATCAG
                  ************************************************************

HsCD00510316      ATGGAAATGATGCGGAGCCTTCGCCATGTAAACATTGATCATCTTCACGTGGGCTGGTAT
NM_003756.2       ATGGAAATGATGCGGAGCCTTCGCCATGTAAACATTGATCATCTTCACGTGGGCTGGTAT
                  ************************************************************

HsCD00510316      CAGTCCACATACTATGGCTCATTCGTTACCCGGGCACTCCTGGACTCTCAGTTTAGTTAC
NM_003756.2       CAGTCCACATACTATGGCTCATTCGTTACCCGGGCACTCCTGGACTCTCAGTTTAGTTAC
                  ************************************************************

HsCD00510316      CAGCATGCCATTGAAGAATCTGTCGTTCTCATTTATGATCCCATAAAAACTGCCCAAGGA
NM_003756.2       CAGCATGCCATTGAAGAATCTGTCGTTCTCATTTATGATCCCATAAAAACTGCCCAAGGA
                  ************************************************************

HsCD00510316      TCTCTCTCACTAAAGGCATACAGACTGACTCCTAAACTGATGGAAGTTTGTAAAGAAAAG
NM_003756.2       TCTCTCTCACTAAAGGCATACAGACTGACTCCTAAACTGATGGAAGTTTGTAAAGAAAAG
                  ************************************************************

HsCD00510316      GATTTTTCCCCTGAAGCATTGAAAAAAGCAAATATCACCTTTGAGTACATGTTTGAAGAA
NM_003756.2       GATTTTTCCCCTGAAGCATTGAAAAAAGCAAATATCACCTTTGAGTACATGTTTGAAGAA
                  ************************************************************

HsCD00510316      GTGCCGATTGTAATTAAAAATTCACATCTGATCAATGTCCTAATGTGGGAACTTGAAAAG
NM_003756.2       GTGCCGATTGTAATTAAAAATTCACATCTGATCAATGTCCTAATGTGGGAACTTGAAAAG
                  ************************************************************

HsCD00510316      AAGTCAGCTGTTGCAGATAAACATGAATTGCTCAGCCTTGCCAGCAGCAATCATTTGGGG
NM_003756.2       AAGTCAGCTGTTGCAGATAAACATGAATTGCTCAGCCTTGCCAGCAGCAATCATTTGGGG
                  ************************************************************

HsCD00510316      AAGAATCTACAGTTGCTGATGGACAGAGTGGATGAAATGAGCCAAGATATAGTTAAATAC
NM_003756.2       AAGAATCTACAGTTGCTGATGGACAGAGTGGATGAAATGAGCCAAGATATAGTTAAATAC
                  ************************************************************

HsCD00510316      AACACATACATGAGGAATACTAGTAAACAACAGCAGCAGAAACATCAGTATCAGCAGCGT
NM_003756.2       AACACATACATGAGGAATACTAGTAAACAACAGCAGCAGAAACATCAGTATCAGCAGCGT
                  ************************************************************

HsCD00510316      CGCCAGCAGGAGAATATGCAGCGCCAGAGCCGAGGAGAACCCCCGCTCCCTGAGGAGGAC
NM_003756.2       CGCCAGCAGGAGAATATGCAGCGCCAGAGCCGAGGAGAACCCCCGCTCCCTGAGGAGGAC
                  ************************************************************

HsCD00510316      CTGTCCAAACTCTTCAAACCACCACAGCCGCCTGCCAGGATGGACTCGCTGCTCATTGCA
NM_003756.2       CTGTCCAAACTCTTCAAACCACCACAGCCGCCTGCCAGGATGGACTCGCTGCTCATTGCA
                  ************************************************************

HsCD00510316      GGCCAGATAAACACTTACTGCCAGAACATCAAGGAGTTCACTGCCCAAAACTTAGGCAAG
NM_003756.2       GGCCAGATAAACACTTACTGCCAGAACATCAAGGAGTTCACTGCCCAAAACTTAGGCAAG
                  ************************************************************

HsCD00510316      CTCTTCATGGCCCAGGCTCTTCAAGAATACAACAAC---
NM_003756.2       CTCTTCATGGCCCAGGCTCTTCAAGAATACAACAACTAA
                  ************************************

Pathways

Reactome :

3' -UTR-mediated translational regulation

Reactome :

Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S

Reactome :

Cap-dependent Translation Initiation

Reactome :

Eukaryotic Translation Initiation

Reactome :

Formation of a pool of free 40S subunits

Reactome :

Formation of the ternary complex, and subsequently, the 43S complex

Reactome :

Gene Expression

Reactome :

GTP hydrolysis and joining of the 60S ribosomal subunit

Reactome :

L13a-mediated translational silencing of Ceruloplasmin expression

Reactome :

Metabolism of proteins

Reactome :

Ribosomal scanning and start codon recognition

Reactome :

Translation

Reactome :

Translation initiation complex formation

WikiPathway :

Translation Factors

Protein

SMART domain :

JAB_MPN : JAB/MPN domain

HPRD :

04885

Clone

Cloning Information :

610157

Original Clone ID :

ccsbBroadEn_01985

Organism

Species Specific ID:

8667

Genome

Chromosome :

Map Location :

8q24.11

Reagents

Labome :

EIF3H-antibody