DNASU Plasmid | MECR (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: MECR

Gene Name: mitochondrial trans-2-enoyl-CoA reductase

Original Clone ID:

ccsbBroadEn_08219

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC001419.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00510890

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1120nts

Open reading frame: 1 to 1120

Reference Sequence Alignment

ATGTGGGTCTGCAGTACCCTGTGGCGGGTGCGAACCCCCGCCCGGCAGTGGCGGGGGCTG
CTCCCAGCTTCTGGCTGTCACGGACCTGCCGCCTCCTCCTACTCCGCATCCGCCGAGCCT
GCCCGGGTCCGGGCGCTTGTCTATGGGCACCACGGGGATCCAGCCAAGGTCGTCGAACTC
AAGAACCTGGAGCTAGCTGCTGTGAGAGGATCAGATGTCCGTGTGAAGATGCTGGCGGCC
CCTATCAATCCATCTGACATAAATATGATCCAAGGAAACTACGGACTCCTTCCTGAACTG
CCTGCTGTTGGAGGGAACGAAGGTGTTGCACAGGTGGTAGCGGTGGGCAGCAATGTGACC
GGGCTGAAGCCAGGAGACTGGGTGATTCCAGCAAATGCTGGTTTAGGAACCTGGCGGACC
GAGGCTGTGTTCAGCGAGGAAGCACTGATCCAAGTTCCGAGTGACATCCCTCTTCAGAGC
GCTGCCACCCTGGGTGTCAATCCCTGCACAGCCTACAGGATGTTGATGGACTTCGAGCAA
CTGCAGCCAGGGGATTCTGTCATCCAGAATGCATCCAACAGCGGAGTGGGGCAAGCGGTC
ATCCAGATCGCCGCAGCCCTGGGCCTAAGAACCATCAATGTGGTCCGAGACAGACCTGAT
ATCCAGAAGCTGAGTGACAGACTGAAGAGTCTGGGGGCTGAGCATGTCATCACAGAAGAG
GAGCTAAGAAGGCCCGAAATGAAAAACTTCTTTAAGGACATGCCCCAGCCACGGCTTGCT
CTCAACTGTGTTGGTGGGAAAAGCTCCACAGAGCTGCTGCGGCAGTTAGCGCGTGGAGGA
ACCATGGTAACCTATGGGGGGATGGCCAAGCAGCCCGTCGTAGCCTCTGTGAGCCTGCTC
ATTTTTAAGGATCTCAAACTTCGAGGCTTTTGGTTGTCCCAGTGGAAGAAGGATCACAGT
CCAGACCAGTTCAAGGAGCTGATCCTCACACTGTGCGATCTCATCCGCCGAGGCCAGCTC
ACAGCCCCTGCCTGCTCCCAGGTCCCGCTGCAGGACTACCAGTCTGCCTTGGAAGCCTCC
ATGAAGCCCTTCATATCTTCAAAGCAGATTCTCACCATG

Protein sequence predicted based on nucleotide sequence

MWVCSTLWRVRTPARQWRGLLPASGCHGPAASSYSASAEPARVRALVYGHHGDPAKVVEL
KNLELAAVRGSDVRVKMLAAPINPSDINMIQGNYGLLPELPAVGGNEGVAQVVAVGSNVT
GLKPGDWVIPANAGLGTWRTEAVFSEEALIQVPSDIPLQSAATLGVNPCTAYRMLMDFEQ
LQPGDSVIQNASNSGVGQAVIQIAAALGLRTINVVRDRPDIQKLSDRLKSLGAEHVITEE
ELRRPEMKNFFKDMPQPRLALNCVGGKSSTELLRQLARGGTMVTYGGMAKQPVVASVSLL
IFKDLKLRGFWLSQWKKDHSPDQFKELILTLCDLIRRGQLTAPACSQVPLQDYQSALEAS
MKPFISSKQILTM

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

MECR

Symbol Nomenclature :

MECR

SYNONYM :

CGI-63; FASN2B; NRBF1

Designation :

homolog of yeast 2-enoyl thioester reductase|mitochondrial 2-enoyl thioester reductase|nuclear receptor binding factor 1|trans-2-enoyl-CoA reductase, mitochondrial

Full Nomenclature :

mitochondrial trans-2-enoyl-CoA reductase

GENEID :

51102

Locus Tag :

RP11-467D18.1

GenBank Accession :

JF432515

HGNC :

19691

MIM :

608205

Vega :

OTTHUMG00000059082

Target GenBank:

JF432515

Reference Sequence Alignment

  

HsCD00510890        ATGTGGGTCTGCAGTACCCTGTGGCGGGTGCGAACCCCCGCCCGGCAGTGGCGGGGGCTG
NM_016011.3         ATGTGGGTCTGCAGTACCCTGTGGCGGGTGCGAACCCCCGCCCGGCAGTGGCGGGGGCTG
XM_005245885.1      ATGTGGGTCTGCAGTACCCTGTGGCGGGTGCGAACCCCCGCCCGGCAGTGGCGGGGGCTG
                    ************************************************************

HsCD00510890        CTCCCAGCTTCTGGCTGTCACGGACCTGCCGCCTCCTCCTACTCCGCATCCGCCGAGCCT
NM_016011.3         CTCCCAGCTTCTGGCTGTCACGGACCTGCCGCCTCCTCCTACTCCGCATCCGCCGAGCCT
XM_005245885.1      CTCCCAGCTTCTGGCTGTCACGGACCTGCCGCCTCCTCCTACTCCGCATCCGCCGAGCCT
                    ************************************************************

HsCD00510890        GCCCGGGTCCGGGCGCTTGTCTATGGGCACCACGGGGATCCAGCCAAGGTCG--------
NM_016011.3         GCCCGGGTCCGGGCGCTTGTCTATGGGCACCACGGGGATCCAGCCAAGGTCG--------
XM_005245885.1      GCCCGGGTCCGGGCGCTTGTCTATGGGCACCACGGGGATCCAGCCAAGGTCGTCGAAGGT
                    ****************************************************        

HsCD00510890        ------------------------------------------------------------
NM_016011.3         ------------------------------------------------------------
XM_005245885.1      ATAACCAGGGAATTGTTCCAGAGGTTTCCTTGGATATTCCTACAACTGATAACAGCTGTC
                                                                                

HsCD00510890        ----------------TCGAACTCAAGAACCTGGAGCTAGCTGCTGTGAGAGGATCAGAT
NM_016011.3         ----------------TCGAACTCAAGAACCTGGAGCTAGCTGCTGTGAGAGGATCAGAT
XM_005245885.1      ATTTCTTCAGCATCTACTGTACTCAAGAACCTGGAGCTAGCTGCTGTGAGAGGATCAGAT
                                      *:****************************************

HsCD00510890        GTCCGTGTGAAGATGCTGGCGGCCCCTATCAATCCATCTGACATAAATATGATCCAAGGA
NM_016011.3         GTCCGTGTGAAGATGCTGGCGGCCCCTATCAATCCATCTGACATAAATATGATCCAAGGA
XM_005245885.1      GTCCGTGTGAAGATGCTGGCGGCCCCTATCAATCCATCTGACATAAATATGATCCAAGGA
                    ************************************************************

HsCD00510890        AACTACGGACTCCTTCCTGAACTGCCTGCTGTTGGAGGGAACGAAGGTGTTGCACAGGTG
NM_016011.3         AACTACGGACTCCTTCCTGAACTGCCTGCTGTTGGAGGGAACGAAGGTGTTGCACAGGTG
XM_005245885.1      AACTACGGATTCCTTCCTGAACTGCCTGCTGTTGGAGGGAACGAAGGTGTTGCACAGGTG
                    ********* **************************************************

HsCD00510890        GTAGCGGTGGGCAGCAATGTGACCGGGCTGAAGCCAGGAGACTGGGTGATTCCAGCAAAT
NM_016011.3         GTAGCGGTGGGCAGCAATGTGACCGGGCTGAAGCCAGGAGACTGGGTGATTCCAGCAAAT
XM_005245885.1      GTAGCGGTGGGCAGCAATGTGACCGGGCTGAAGCCAGGAGACTGGGTGATTCCAGCAAAT
                    ************************************************************

HsCD00510890        GCTGGTTTAGGAACCTGGCGGACCGAGGCTGTGTTCAGCGAGGAAGCACTGATCCAAGTT
NM_016011.3         GCTGGTTTAGGAACCTGGCGGACCGAGGCTGTGTTCAGCGAGGAAGCACTGATCCAAGTT
XM_005245885.1      GCTGGTTTAGGAACCTGGCGGACCGAGGCTGTGTTCAGCGAGGAAGCACTGATCCAAGTT
                    ************************************************************

HsCD00510890        CCGAGTGACATCCCTCTTCAGAGCGCTGCCACCCTGGGTGTCAATCCCTGCACAGCCTAC
NM_016011.3         CCGAGTGACATCCCTCTTCAGAGCGCTGCCACCCTGGGTGTCAATCCCTGCACAGCCTAC
XM_005245885.1      CCGAGTGACATCCCTCTTCAGAGCGCTGCCACCCTGGGTGTCAATCCCTGCACAGCCTAC
                    ************************************************************

HsCD00510890        AGGATGTTGATGGACTTCGAGCAACTGCAGCCAGGGGATTCTGTCATCCAGAATGCATCC
NM_016011.3         AGGATGTTGATGGACTTCGAGCAACTGCAGCCAGGGGATTCTGTCATCCAGAATGCATCC
XM_005245885.1      AGGATGTTGATGGACTTCGAGCAACTGCAGCCAGGGGATTCTGTCATCCAGAATGCATCC
                    ************************************************************

HsCD00510890        AACAGCGGAGTGGGGCAAGCGGTCATCCAGATCGCCGCAGCCCTGGGCCTAAGAACCATC
NM_016011.3         AACAGCGGAGTGGGGCAAGCGGTCATCCAGATCGCCGCAGCCCTGGGCCTAAGAACCATC
XM_005245885.1      AACAGCGGAGTGGGGCAAGCAGTCATCCAGATCGCCGCAGCCCTGGGCCTAAGAACCATC
                    ********************.***************************************

HsCD00510890        AATGTGGTCCGAGACAGACCTGATATCCAGAAGCTGAGTGACAGACTGAAGAGTCTGGGG
NM_016011.3         AATGTGGTCCGAGACAGACCTGATATCCAGAAGCTGAGTGACAGACTGAAGAGTCTGGGG
XM_005245885.1      AATGTGGTCCGAGACAGACCTGATATCCAGAAGCTGAGTGACAGACTGAAGAGTCTGGGG
                    ************************************************************

HsCD00510890        GCTGAGCATGTCATCACAGAAGAGGAGCTAAGAAGGCCCGAAATGAAAAACTTCTTTAAG
NM_016011.3         GCTGAGCATGTCATCACAGAAGAGGAGCTAAGAAGGCCCGAAATGAAAAACTTCTTTAAG
XM_005245885.1      GCTGAGCATGTCATCACAGAAGAGGAGCTAAGAAGGCCCGAAATGAAAAACTTCTTTAAG
                    ************************************************************

HsCD00510890        GACATGCCCCAGCCACGGCTTGCTCTCAACTGTGTTGGTGGGAAAAGCTCCACAGAGCTG
NM_016011.3         GACATGCCCCAGCCACGGCTTGCTCTCAACTGTGTTGGTGGGAAAAGCTCCACAGAGCTG
XM_005245885.1      GACATGCCCCAGCCACGGCTTGCTCTCAACTGTGTTGGTGGGAAAAGCTCCACAGAGCTG
                    ************************************************************

HsCD00510890        CTGCGGCAGTTAGCGCGTGGAGGAACCATGGTAACCTATGGGGGGATGGCCAAGCAGCCC
NM_016011.3         CTGCGGCAGTTAGCGCGTGGAGGAACCATGGTAACCTATGGGGGGATGGCCAAGCAGCCC
XM_005245885.1      CTGCGGCAGTTAGCGCGTGGAGGAACCATGGTAACCTATGGGGGGATGGCCAAGCAGCCC
                    ************************************************************

HsCD00510890        GTCGTAGCCTCTGTGAGCCTGCTCATTTTTAAGGATCTCAAACTTCGAGGCTTTTGGTTG
NM_016011.3         GTCGTAGCCTCTGTGAGCCTGCTCATTTTTAAGGATCTCAAACTTCGAGGCTTTTGGTTG
XM_005245885.1      GTCGTAGCCTCTGTGAGCCTGCTCATTTTTAAGGATCTCAAACTTCGAGGCTTTTGGTTG
                    ************************************************************

HsCD00510890        TCCCAGTGGAAGAAGGATCACAGTCCAGACCAGTTCAAGGAGCTGATCCTCACACTGTGC
NM_016011.3         TCCCAGTGGAAGAAGGATCACAGTCCAGACCAGTTCAAGGAGCTGATCCTCACACTGTGC
XM_005245885.1      TCCCAGTGGAAGAAGGATCACAGTCCAGACCAGTTCAAGGAGCTGATCCTCACACTGTGC
                    ************************************************************

HsCD00510890        GATCTCATCCGCCGAGGCCAGCTCACAGCCCCTGCCTGCTCCCAGGTCCCGCTGCAGGAC
NM_016011.3         GATCTCATCCGCCGAGGCCAGCTCACAGCCCCTGCCTGCTCCCAGGTCCCGCTGCAGGAC
XM_005245885.1      GATCTCATCCGCCGAGGCCAGCTCACAGCCCCTGCCTGCTCCCAGGTCCCGCTGCAGGAC
                    ************************************************************

HsCD00510890        TACCAGTCTGCCTTGGAAGCCTCCATGAAGCCCTTCATATCTTCAAAGCAGATTCTCACC
NM_016011.3         TACCAGTCTGCCTTGGAAGCCTCCATGAAGCCCTTCATATCTTCAAAGCAGATTCTCACC
XM_005245885.1      TACCAGTCTGCCTTGGAAGCCTCCATGAAGCCCTTCATATCTTCAAAGCAGATTCTCACC
                    ************************************************************

HsCD00510890        ATG---
NM_016011.3         ATGTGA
XM_005245885.1      ATGTGA
                    ***