DNASU Plasmid | LASS3 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: CERS3

Gene Name: ceramide synthase 3

Original Clone ID:

ccsbBroadEn_09831

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC034970.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00510967

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1150nts

Open reading frame: 1 to 1150

Reference Sequence Alignment

ATGTTTTGGACGTTTAAAGAATGGTTCTGGTTGGAAAGATTCTGGCTTCCTCCAACAATA
AAGTGGTCAGATCTTGAGGATCACGATGGACTCGTCTTTGTAAAACCTTCTCATTTATAC
GTGACAATTCCATATGCTTTTCTCTTGCTGATTATCAGGCGTGTATTTGAAAAATTTGTT
GCTTCACCTCTAGCAAAATCATTTGGCATTAAAGAGACAGTTCGAAAGGTTACACCAAAT
ACTGTCTTAGAGAATTTTTTCAAACATTCCACAAGGCAACCATTGCAAACTGATATTTAT
GGACTGGCAAAGAAGTGTAACTTGACGGAGCGCCAGGTGGAAAGATGGTTTAGGAGTCGG
CGGAATCAAGAGAGGCCTTCCAGGCTGAAGAAATTCCAGGAAGCTTGCTGGAGATTTGCA
TTTTACTTAATGATCACTGTTGCTGGAATTGCGTTTCTTTATGATAAACCTTGGCTATAT
GACTTATGGGAGGTTTGGAATGGCTATCCCAAACAGCCCCTGCTGCCATCCCAGTACTGG
TACTACATTTTAGAAATGAGTTTTTATTGGTCTCTGTTATTTAGACTTGGCTTTGATGTC
AAGAGAAAGGATTTTCTAGCTCATATCATCCACCACCTGGCTGCTATTAGTCTGATGAGC
TTCTCTTGGTGTGCTAATTATATTCGCAGTGGGACCCTCGTGATGATTGTACACGATGTG
GCTGACATTTGGCTGGAGTCTGCTAAGATGTTTTCTTATGCTGGATGGACGCAGACCTGT
AACACCCTGTTTTTCATCTTCTCCACCATATTTTTCATCAGCCGCCTCATTGTTTTTCCT
TTCTGGATTTTATATTGCACGCTGATCTTGCCTATGTATCACCTCGAGCCTTTCTTTTCA
TACATCTTCCTCAACCTACAGCTCATGATCTTGCAGGTCCTTCACCTTTACTGGGGTTAT
TACATCTTGAAGATGCTCAACAGATGTATATTCATGAAGAGCATCCAGGATGTGAGGAGT
GATGACGAGGATTATGAAGAGGAAGAGGAAGAGGAAGAAGAAGAGGCTACCAAAGGCAAA
GAGATGGATTGTTTAAAGAACGGCCTCGGGGCTGAGAGGCACCTCATTCCCAATGGCCAG
CATGGCCAT

Protein sequence predicted based on nucleotide sequence

MFWTFKEWFWLERFWLPPTIKWSDLEDHDGLVFVKPSHLYVTIPYAFLLLIIRRVFEKFV
ASPLAKSFGIKETVRKVTPNTVLENFFKHSTRQPLQTDIYGLAKKCNLTERQVERWFRSR
RNQERPSRLKKFQEACWRFAFYLMITVAGIAFLYDKPWLYDLWEVWNGYPKQPLLPSQYW
YYILEMSFYWSLLFRLGFDVKRKDFLAHIIHHLAAISLMSFSWCANYIRSGTLVMIVHDV
ADIWLESAKMFSYAGWTQTCNTLFFIFSTIFFISRLIVFPFWILYCTLILPMYHLEPFFS
YIFLNLQLMILQVLHLYWGYYILKMLNRCIFMKSIQDVRSDDEDYEEEEEEEEEEATKGK
EMDCLKNGLGAERHLIPNGQHGH

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

CERS3

Symbol Nomenclature :

CERS3

SYNONYM :

ARCI9; LASS3

Designation :

LAG1 homolog, ceramide synthase 3|LAG1 longevity assurance homolog 3|dihydroceramide synthase 3

Full Nomenclature :

ceramide synthase 3

GENEID :

204219

GenBank Accession :

EU176496

HGNC :

23752

MIM :

615276

Vega :

OTTHUMG00000172568

Target GenBank:

EU176496

Reference Sequence Alignment

  

HsCD00510967      ATGTTTTGGACGTTTAAAGAATGGTTCTGGTTGGAAAGATTCTGGCTTCCTCCAACAATA
NM_178842.3       ATGTTTTGGACGTTTAAAGAATGGTTCTGGTTGGAAAGATTCTGGCTTCCTCCAACAATA
                  ************************************************************

HsCD00510967      AAGTGGTCAGATCTTGAGGATCACGATGGACTCGTCTTTGTAAAACCTTCTCATTTATAC
NM_178842.3       AAGTGGTCAGATCTTGAGGATCACGATGGACTCGTCTTTGTAAAACCTTCTCATTTATAC
                  ************************************************************

HsCD00510967      GTGACAATTCCATATGCTTTTCTCTTGCTGATTATCAGGCGTGTATTTGAAAAATTTGTT
NM_178842.3       GTGACAATTCCATATGCTTTTCTCTTGCTGATTATCAGACGTGTATTTGAAAAATTTGTT
                  **************************************.*********************

HsCD00510967      GCTTCACCTCTAGCAAAATCATTTGGCATTAAAGAGACAGTTCGAAAGGTTACACCAAAT
NM_178842.3       GCTTCACCTCTAGCAAAATCATTTGGCATTAAAGAGACAGTTCGAAAGGTTACACCAAAT
                  ************************************************************

HsCD00510967      ACTGTCTTAGAGAATTTTTTCAAACATTCCACAAGGCAACCATTGCAAACTGATATTTAT
NM_178842.3       ACTGTCTTAGAGAATTTTTTCAAACATTCCACAAGGCAACCATTGCAAACTGATATTTAT
                  ************************************************************

HsCD00510967      GGACTGGCAAAGAAGTGTAACTTGACGGAGCGCCAGGTGGAAAGATGGTTTAGGAGTCGG
NM_178842.3       GGACTGGCAAAGAAGTGTAACTTGACGGAGCGCCAGGTGGAAAGATGGTTTAGGAGTCGG
                  ************************************************************

HsCD00510967      CGGAATCAAGAGAGGCCTTCCAGGCTGAAGAAATTCCAGGAAGCTTGCTGGAGATTTGCA
NM_178842.3       CGGAATCAAGAGAGGCCTTCCAGGCTGAAGAAATTCCAGGAAGCTTGCTGGAGATTTGCA
                  ************************************************************

HsCD00510967      TTTTACTTAATGATCACTGTTGCTGGAATTGCGTTTCTTTATGATAAACCTTGGCTATAT
NM_178842.3       TTTTACTTAATGATCACTGTTGCTGGAATTGCGTTTCTTTATGATAAACCTTGGCTATAT
                  ************************************************************

HsCD00510967      GACTTATGGGAGGTTTGGAATGGCTATCCCAAACAGCCCCTGCTGCCATCCCAGTACTGG
NM_178842.3       GACTTATGGGAGGTTTGGAATGGCTATCCCAAACAGCCCCTGCTGCCATCCCAGTACTGG
                  ************************************************************

HsCD00510967      TACTACATTTTAGAAATGAGTTTTTATTGGTCTCTGTTATTTAGACTTGGCTTTGATGTC
NM_178842.3       TACTACATTTTAGAAATGAGTTTTTATTGGTCTCTGTTATTTAGACTTGGCTTTGATGTC
                  ************************************************************

HsCD00510967      AAGAGAAAGGATTTTCTAGCTCATATCATCCACCACCTGGCTGCTATTAGTCTGATGAGC
NM_178842.3       AAGAGAAAGGATTTTCTAGCTCATATCATCCACCACCTGGCTGCTATTAGTCTGATGAGC
                  ************************************************************

HsCD00510967      TTCTCTTGGTGTGCTAATTATATTCGCAGTGGGACCCTCGTGATGATTGTACACGATGTG
NM_178842.3       TTCTCTTGGTGTGCTAATTATATTCGCAGTGGGACCCTCGTGATGATTGTACACGATGTG
                  ************************************************************

HsCD00510967      GCTGACATTTGGCTGGAGTCTGCTAAGATGTTTTCTTATGCTGGATGGACGCAGACCTGT
NM_178842.3       GCTGACATTTGGCTGGAGTCTGCTAAGATGTTTTCTTATGCTGGATGGACGCAGACCTGT
                  ************************************************************

HsCD00510967      AACACCCTGTTTTTCATCTTCTCCACCATATTTTTCATCAGCCGCCTCATTGTTTTTCCT
NM_178842.3       AACACCCTGTTTTTCATCTTCTCCACCATATTTTTCATCAGCCGCCTCATTGTTTTTCCT
                  ************************************************************

HsCD00510967      TTCTGGATTTTATATTGCACGCTGATCTTGCCTATGTATCACCTCGAGCCTTTCTTTTCA
NM_178842.3       TTCTGGATTTTATATTGCACGCTGATCTTGCCTATGTATCACCTCGAGCCTTTCTTTTCA
                  ************************************************************

HsCD00510967      TACATCTTCCTCAACCTACAGCTCATGATCTTGCAGGTCCTTCACCTTTACTGGGGTTAT
NM_178842.3       TACATCTTCCTCAACCTACAGCTCATGATCTTGCAGGTCCTTCACCTTTACTGGGGTTAT
                  ************************************************************

HsCD00510967      TACATCTTGAAGATGCTCAACAGATGTATATTCATGAAGAGCATCCAGGATGTGAGGAGT
NM_178842.3       TACATCTTGAAGATGCTCAACAGATGTATATTCATGAAGAGCATCCAGGATGTGAGGAGT
                  ************************************************************

HsCD00510967      GATGACGAGGATTATGAAGAGGAAGAGGAAGAGGAAGAAGAAGAGGCTACCAAAGGCAAA
NM_178842.3       GATGACGAGGATTATGAAGAGGAAGAGGAAGAGGAAGAAGAAGAGGCTACCAAAGGCAAA
                  ************************************************************

HsCD00510967      GAGATGGATTGTTTAAAGAACGGCCTCGGGGCTGAGAGGCACCTCATTCCCAATGGCCAG
NM_178842.3       GAGATGGATTGTTTAAAGAACGGCCTCAGGGCTGAGAGGCACCTCATTCCCAATGGCCAG
                  ***************************.********************************

HsCD00510967      CATGGCCAT---
NM_178842.3       CATGGCCATTAG
                  *********