DNASU Plasmid | CPSF3L (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: CPSF3L

Gene Name: cleavage and polyadenylation specific factor 3-like

Original Clone ID:

ccsbBroadEn_12126

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC008041.2,BC020199.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511087

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1168nts

Open reading frame: 1 to 1168

Reference Sequence Alignment

ATGCCTGAGATCAGAGTCACGCCCTTGGGGGCCGGCCAGGACGTGGGCCGAAGCTGCATC
CTGGTCTCCATTGCGGGCAAGAATGTCATGCTGGACTGTGGAATGCACATGGGCTTCAAT
GACGACCGACGCTTCCCTGACTTCTCCTACATCACCCAGAACGGCCGCCTAACAGACTTC
CTGGACTGTGTGATCATTAGCCACTTCCACCTGGACCACTGCGGGGCACTCCCCTACTTC
AGCGAGATGGTGGGCTACGACGGCCCCATCTACATGACTCACCCCACCCAGGCCATCTGC
CCCATCTTGCTGGAGGACTACCGCAAGATCGCCGTAGACAAGAAGGGCGAGGCCAACTTC
TTCACCTCCCAGATGATCAAAGACTGCATCCTCCTGGAGACCTTCTGGGAGCGCATGAAC
CTGAAGGTGCCCATCTACTTCTCCACGGGGCTGACCGAGAAGGCCAACCACTACTACAAG
CTGTTCATCCCCTGGACCAACCAGAAGATCCGCAAGACTTTTGTGCAGAGGAACATGTTT
GAGTTCAAGCACATCAAGGCCTTCGACCGGGCTTTTGCTGACAACCCAGGACCGATGGTT
GTGTTTGCCACGCCAGGAATGCTGCACGCTGGGCAGTCCCTGCAGATCTTCCGGAAATGG
GCCGGAAACGAAAAGAACATGGTCATCATGCCCGGCTACTGCGTGCAGGGCACCGTCGGC
CACAAGATCCTCAGCGGGCAGCGGAAGCTCGAGATGGAGGGGCGGCAGGTGCTGGAGGTC
AAGATGCAGGTGGAGTACATGTCATTCAGCGCACACGCGGACGCCAAGGGCATCATGCAG
CTGGTGTCCTCAGAGCAAGCCCTCAAAGAGCTGGGTCTGGCTGAGCACCAGCTGCGCTTC
ACCTGCCGCGTGCACCTGCATGACACACGCAAGGAGCAGGAGACGGCATTGCGCGTCTAC
AGCCACCTCAAGAGCGTCCTGAAGGACCACTGTGTGCAGCACCTCCCGGACGGCTCTGTG
ACTGTGGAGTCCGTCCTCCTCCAGGCCGCCGCCCCTTCTGAGGACCCAGGCACCAAGGTG
CTGCTGGTCTCCTGGACCTACCAGGACGAGGAGCTGGGGAGCTTCCTCACATCTCTGCTG
AAGAAGGGCCTCCCCCAGGCCCCCAGC

Protein sequence predicted based on nucleotide sequence

MPEIRVTPLGAGQDVGRSCILVSIAGKNVMLDCGMHMGFNDDRRFPDFSYITQNGRLTDF
LDCVIISHFHLDHCGALPYFSEMVGYDGPIYMTHPTQAICPILLEDYRKIAVDKKGEANF
FTSQMIKDCILLETFWERMNLKVPIYFSTGLTEKANHYYKLFIPWTNQKIRKTFVQRNMF
EFKHIKAFDRAFADNPGPMVVFATPGMLHAGQSLQIFRKWAGNEKNMVIMPGYCVQGTVG
HKILSGQRKLEMEGRQVLEVKMQVEYMSFSAHADAKGIMQLVSSEQALKELGLAEHQLRF
TCRVHLHDTRKEQETALRVYSHLKSVLKDHCVQHLPDGSVTVESVLLQAAAPSEDPGTKV
LLVSWTYQDEELGSFLTSLLKKGLPQAPS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

CPSF3L

Symbol Nomenclature :

CPSF3L

SYNONYM :

CPSF73L; INT11; INTS11; RC-68; RC68

Designation :

CPSF3-like protein|cleavage and polyadenylation-specific factor 3-like protein|integrator complex subunit 11|protein related to CPSF subunits of 68 kDa|related to CPSF subunits 68 kDa

Full Nomenclature :

cleavage and polyadenylation specific factor 3-like

GENEID :

54973

Locus Tag :

RP5-890O3.8

GenBank Accession :

AM393218

HGNC :

26052

MIM :

611354

Vega :

OTTHUMG00000003330

Target GenBank:

AM393218

Reference Sequence Alignment

  

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      ------------------------------------------------------------
NM_017871.5         ------------------------------------------------------------
NM_001256460.1      ATGCTGGACTGTGGAATGCACATGGGCTTCAATGACGACCGACGCTTCCCTGACTTCTCC
NM_001256456.1      ------------------------------------------------------------
NM_001256462.1      ------------------------------------------------------------
                                                                                

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      ------------------------------------------------------------
NM_017871.5         ------------------------------------------------------------
NM_001256460.1      TACATCACCCAGAACGGCCGCCTAACAGACTTCCTGGACTGTGTGATCATTAGCCACTTC
NM_001256456.1      ------------------------------------------------------------
NM_001256462.1      ------------------------------------------------------------
                                                                                

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      ------------------------------------------------------------
NM_017871.5         ------------------------------------------------------------
NM_001256460.1      CACCTGGACCACTGCGGGGCACTCCCCTACTTCAGCGAGATGGTGGGCTACGACGGGCCC
NM_001256456.1      ------------------------------------------------------------
NM_001256462.1      ------------------------------------------------------------
                                                                                

HsCD00511087        ---------------------------ATGCCTGAGATCAGAGTCACGCCCTTGGGGGCC
NM_001256463.1      ---------------------------ATGCCTGAGATCAGAGTCACGCCCTTGGGGGCC
NM_017871.5         ---------------------------ATGCCTGAGATCAGAGTCACGCCCTTGGGGGCC
NM_001256460.1      ATCTACATGACTCACCCCACCCAGGCCATCTGCCCCATCTTGCTGGAGGACTACCGCAAG
NM_001256456.1      ---------ATGTGTGGTGCTGGCTTTGGACACTTCGAGTGGCTGGCAGGGGGAGGGGCC
NM_001256462.1      ---------------------------ATGCCTGAGATCAGAGTCACGCCCTTGGGTGCC
                                               .        .: : . * ...       * .. 

HsCD00511087        GGCCAGGACGTGGGCCGAAGCTGCATCCTGGTCTCCATTGCGGGCAAGAATGTCATGCTG
NM_001256463.1      GGCCAGGACGTGGGCCGAAGCTGCATCCTGGTCTCCATTGCGGGCAAGAATGTCATGCTG
NM_017871.5         GGCCAGGACGTGGGCCGAAGCTGCATCCTGGTCTCCATTGCGGGCAAGAATGTCATGCTG
NM_001256460.1      ATCGCCGTAGACAAGAAGGGCGAGGCCAACTTCTTCACCTCCCAGATGATCAAAGACTGC
NM_001256456.1      GGCCAGGACGTGGGCCGAAGCTGCATCCTGGTCTCCATTGCGGGCAAGAATGTCATGCTG
NM_001256462.1      TGCCCTGTGCTGTCCTGGAACCTGGTCCTTCCACCGGGCAGGGGGTCGAGGGCACCTTCC
                      * . *:  :     ....*   . *.:   .   .      . : **  . .      

HsCD00511087        GACTGTGGAATGCACATGGGCTTCAATGACGACCGACG----------------------
NM_001256463.1      GACTGTGGAATGCACATGGGCTTCAATGACGACGTAG-----------------------
NM_017871.5         GACTGTGGAATGCACATGGGCTTCAATGACGACCGACG----------------------
NM_001256460.1      ATGAAGAAGGTGGTGGCTGTCCACCTCCACCAGACGG-----------------------
NM_001256456.1      GACTGTGGAATGCACATGGGCTTCAATGACGACCGACGCTTCCCTGACTTCTCCTACATC
NM_001256462.1      AGCACAGACACCAGGACGGGATTCCATGTCCTCCCCC-----------------------
                    .  :  .. .     .  * . :*.:  :* :                            

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      ------------------------------------------------------------
NM_017871.5         ------------------------------------------------------------
NM_001256460.1      ------------------------------------------------------------
NM_001256456.1      ACCCAGAACGGCCGCCTAACAGACTTCCTGGACTGTGTGATCATTAGCCACTTCCACCTG
NM_001256462.1      ------------------------------------------------------------
                                                                                

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      ------------------------------------------------------------
NM_017871.5         ------------------------------------------------------------
NM_001256460.1      ------------------------------------------------------------
NM_001256456.1      GACCACTGCGGGGCACTCCCCTACTTCAGCGAGATGGTGGGCTACGACGGGCCCATCTAC
NM_001256462.1      ------------------------------------------------------------
                                                                                

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      ------------------------------------------------------------
NM_017871.5         ------------------------------------------------------------
NM_001256460.1      ------------------------------------------------------------
NM_001256456.1      ATGACTCACCCCACCCAGGCCATCTGCCCCATCTTGCTGGAGGACTACCGCAAGATCGCC
NM_001256462.1      ------------------------------------------------------------
                                                                                

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      ------------------------------------------------------------
NM_017871.5         ------------------------------------------------------------
NM_001256460.1      ------------------------------------------------------------
NM_001256456.1      GTAGACAAGAAGGGCGAGGCCAACTTCTTCACCTCCCAGATGATCAAAGACTGCATGAAG
NM_001256462.1      ------------------------------------------------------------
                                                                                

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      -------------------------------ATG---------ATGAGCTGGAGATCAAG
NM_017871.5         ------------------------------------------------------------
NM_001256460.1      -------------------------------TCCAGGTAGATGATGAGCTGGAGATCAAG
NM_001256456.1      AAGGTGGTGGCTGTCCACCTCCACCAGACGGTCCAGGTAGATGATGAGCTGGAGATCAAG
NM_001256462.1      -------------------------------ATGGCGTGAATGATGAGCTGGAGATCAAG
                                                                                

HsCD00511087        -----------------------CTTCCCTGACTTCTCCTACATCACCCAGAACGGCCGC
NM_001256463.1      GCCTACTATGCAGGCCACGTGCTGGGGGCAGCCATGTTCCAGATTAAAGTGGGCTCAGAG
NM_017871.5         -----------------------CTTCCCTGACTTCTCCTACATCACCCAGAACGGCCGC
NM_001256460.1      GCCTACTATGCAGGCCACGTGCTGGGGGCAGCCATGTTCCAGATTAAAGTGGGCTCAGAG
NM_001256456.1      GCCTACTATGCAGGCCACGTGCTGGGGGCAGCCATGTTCCAGATTAAAGTGGGCTCAGAG
NM_001256462.1      GCCTACTATGCAGGCCACGTGCTGGGGGCAGCCATGTTCCAGATTAAAGTGGGCTCAGAG
                                                *:*.*:* * * * ** *.. :*..*  . . 

HsCD00511087        CTAACAGACTTCCTGGACTGTGTGATCATTAGCCACTTCCACCTGGACCACTGCGGGGCA
NM_001256463.1      TCTGTGGTCTACACGGGTGATTATAACATGACCCCAGACCGACACTTAGGAGCTGCCTGG
NM_017871.5         CTAACAGACTTCCTGGACTGTGTGATCATTAGCCACTTCCACCTGGACCACTGCGGGGCA
NM_001256460.1      TCTGTGGTCTACACGGGTGATTATAACATGACCCCAGACCGACACTTAGGAGCTGCCTGG
NM_001256456.1      TCTGTGGTCTACACGGGTGATTATAACATGACCCCAGACCGACACTTAGGAGCTGCCTGG
NM_001256462.1      TCTGTGGTCTACACGGGTGATTATAACATGACCCCAGACCGACACTTAGGAGCTGCCTGG
                      :. .*:**:*. **.  .* : *:*** * **.. :**..*:  :. ..   *    .

HsCD00511087        CTCCCCTACTTCAGCGAGATGGTGGGCTACGACGGCCCCATCTACATGACTCACCCCACC
NM_001256463.1      ATTGACAAGTGCCGCCCCAACCTGCTCATCACAGAGTCCACGTACGCCACGACCATCCGT
NM_017871.5         CTCCCCTACTTCAGCGAGATGGTGGGCTACGACGGGCCCATCTACATGACTCACCCCACC
NM_001256460.1      ATTGACAAGTGCCGCCCCAACCTGCTCATCACAGAGTCCACGTACGCCACGACCATCCGT
NM_001256456.1      ATTGACAAGTGCCGCCCCAACCTGCTCATCACAGAGTCCACGTACGCCACGACCATCCGT
NM_001256462.1      ATTGACAAGTGCCGCCCCAACCTGCTCATCACAGAGTCCACGTACGCCACGACCATCCGT
                    .*  .*:* * *.** . *:  **  *::*...*.  ***  ***.  ** ..*. *.  

HsCD00511087        CAGGCCATCTGCCCCATCTTGCTGGAGGACTACCGCAAGATCGCCGTAGACAAGAAGGGC
NM_001256463.1      GACTCCAAGCGCTGCCGGGAGCGAGACTTCCTGAAGAAAGTCCACGAGACCGTGGAGCGT
NM_017871.5         CAGGCCATCTGCCCCATCTTGCTGGAGGACTACCGCAAGATCGCCGTAGACAAGAAGGGC
NM_001256460.1      GACTCCAAGCGCTGCCGGGAGCGAGACTTCCTGAAGAAAGTCCACGAGACCGTGGAGCGT
NM_001256456.1      GACTCCAAGCGCTGCCGGGAGCGAGACTTCCTGAAGAAAGTCCACGAGACCGTGGAGCGT
NM_001256462.1      GACTCCAAGCGCTGCCGGGAGCGAGACTTCCTGAAGAAAGTCCACGAGACCGTGGAGCGT
                     *  ***:  **  *.   :** .**  :* : .. **..** .**:...*.:*.** * 

HsCD00511087        GAGGCCAACTTCTTCACCTCCCAGATGATCAAAGACTGCAT-------------------
NM_001256463.1      GGTGGGAAGGTGCTGATACCTGTGTTCGCGCTGGGCCGCGCCCAGGAGCTCTG-------
NM_017871.5         GAGGCCAACTTCTTCACCTCCCAGATGATCAAAGACTGCATGAAGAAGGTGGTGGCTGTC
NM_001256460.1      GGTGGGAAGGTGCTGATACCTGTGTTCGCGCTGGGCCGCGCCCAGGAGCTCTG-------
NM_001256456.1      GGTGGGAAGGTGCTGATACCTGTGTTCGCGCTGGGCCGCGCCCAGGAGCTCTG-------
NM_001256462.1      GGTGGGAAGGTGCTGATACCTGTGTTCGCGCTGGGCCGCGCCCAGGAGCTCTG-------
                    *. *  **  *  * * . *  :*:* .  .:.*.* **.                    

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      ------------------------------------------------------------
NM_017871.5         CACCTCCACCAGACGGTCCAGGTAGATGATGAGCTGGAGATCAAGGCCTACTATGCAGGC
NM_001256460.1      ------------------------------------------------------------
NM_001256456.1      ------------------------------------------------------------
NM_001256462.1      ------------------------------------------------------------
                                                                                

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      ------------------------------------------------------------
NM_017871.5         CACGTGCTGGGGGCAGCCATGTTCCAGATTAAAGTGGGCTCAGAGTCTGTGGTCTACACG
NM_001256460.1      ------------------------------------------------------------
NM_001256456.1      ------------------------------------------------------------
NM_001256462.1      ------------------------------------------------------------
                                                                                

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      ------------------------------------------------------------
NM_017871.5         GGTGATTATAACATGACCCCAGACCGACACTTAGGAGCTGCCTGGATTGACAAGTGCCGC
NM_001256460.1      ------------------------------------------------------------
NM_001256456.1      ------------------------------------------------------------
NM_001256462.1      ------------------------------------------------------------
                                                                                

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      ------------------------------------------------------------
NM_017871.5         CCCAACCTGCTCATCACAGAGTCCACGTACGCCACGACCATCCGTGACTCCAAGCGCTGC
NM_001256460.1      ------------------------------------------------------------
NM_001256456.1      ------------------------------------------------------------
NM_001256462.1      ------------------------------------------------------------
                                                                                

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      ------------------------------------------------------------
NM_017871.5         CGGGAGCGAGACTTCCTGAAGAAAGTCCACGAGACCGTGGAGCGTGGTGGGAAGGTGCTG
NM_001256460.1      ------------------------------------------------------------
NM_001256456.1      ------------------------------------------------------------
NM_001256462.1      ------------------------------------------------------------
                                                                                

HsCD00511087        -----------------------------------------CCTCCTGGAGACCTTCTGG
NM_001256463.1      --------------------------------------CATCCTCCTGGAGACCTTCTGG
NM_017871.5         ATACCTGTGTTCGCGCTGGGCCGCGCCCAGGAGCTCTGCATCCTCCTGGAGACCTTCTGG
NM_001256460.1      --------------------------------------CATCCTCCTGGAGACCTTCTGG
NM_001256456.1      --------------------------------------CATCCTCCTGGAGACCTTCTGG
NM_001256462.1      --------------------------------------CATCCTCCTGGAGACCTTCTGG
                                                             *******************

HsCD00511087        GAGCGCATGAACCTGAAGGTGCCCATCTACTTCTCCACGGGGCTGACCGAGAAGGCCAAC
NM_001256463.1      GAGCGCATGAACCTGAAGGTGCCCATCTACTTCTCCACGGGGCTGACCGAGAAGGCCAAC
NM_017871.5         GAGCGCATGAACCTGAAGGTGCCCATCTACTTCTCCACGGGGCTGACCGAGAAGGCCAAC
NM_001256460.1      GAGCGCATGAACCTGAAGGTGCCCATCTACTTCTCCACGGGGCTGACCGAGAAGGCCAAC
NM_001256456.1      GAGCGCATGAACCTGAAGGTGCCCATCTACTTCTCCACGGGGCTGACCGAGAAGGCCAAC
NM_001256462.1      GAGCGCATGAACCTGAAGGTGCCCATCTACTTCTCCACGGGGCTGACCGAGAAGGCCAAC
                    ************************************************************

HsCD00511087        CACTACTACAAGCTGTTCATCCCCTGGACCAACCAGAAGATCCGCAAGACTTTTGTGCAG
NM_001256463.1      CACTACTACAAGCTGTTCATCCCCTGGACCAACCAGAAGATCCGCAAGACTTTCGTGCAG
NM_017871.5         CACTACTACAAGCTGTTCATCCCCTGGACCAACCAGAAGATCCGCAAGACTTTCGTGCAG
NM_001256460.1      CACTACTACAAGCTGTTCATCCCCTGGACCAACCAGAAGATCCGCAAGACTTTCGTGCAG
NM_001256456.1      CACTACTACAAGCTGTTCATCCCCTGGACCAACCAGAAGATCCGCAAGACTTTCGTGCAG
NM_001256462.1      CACTACTACAAGCTGTTCATCCCCTGGACCAACCAGAAGATCCGCAAGACTTTCGTGCAG
                    ***************************************************** ******

HsCD00511087        AGGAACATGTTTGAGTTCAAGCACATCAAGGCCTTCGACCGGGCTTTTGCTGACAACCCA
NM_001256463.1      AGGAACATGTTTGAGTTCAAGCACATCAAGGCCTTCGACCGGGCTTTTGCTGACAACCCA
NM_017871.5         AGGAACATGTTTGAGTTCAAGCACATCAAGGCCTTCGACCGGGCTTTTGCTGACAACCCA
NM_001256460.1      AGGAACATGTTTGAGTTCAAGCACATCAAGGCCTTCGACCGGGCTTTTGCTGACAACCCA
NM_001256456.1      AGGAACATGTTTGAGTTCAAGCACATCAAGGCCTTCGACCGGGCTTTTGCTGACAACCCA
NM_001256462.1      AGGAACATGTTTGAGTTCAAGCACATCAAGGCCTTCGACCGGGCTTTTGCTGACAACCCA
                    ************************************************************

HsCD00511087        GGACCGATGGTTGTGTTTGCCACGCCAGGAATGCTGCACGCTGGGCAGTCCCTGCAGATC
NM_001256463.1      GGACCGATGGTTGTGTTTGCCACGCCAGGAATGCTGCACGCTGGGCAGTCCCTGCAGATC
NM_017871.5         GGACCGATGGTTGTGTTTGCCACGCCAGGAATGCTGCACGCTGGGCAGTCCCTGCAGATC
NM_001256460.1      GGACCGATGGTTGTGTTTGCCACGCCAGGAATGCTGCACGCTGGGCAGTCCCTGCAGATC
NM_001256456.1      GGACCGATGGTTGTGTTTGCCACGCCAGGAATGCTGCACGCTGGGCAGTCCCTGCAGATC
NM_001256462.1      GGACCGATGGTTGTGTTTGCCACGCCAGGAATGCTGCACGCTGGGCAGTCCCTGCAGATC
                    ************************************************************

HsCD00511087        TTCCGGAAATGGGCCGGAAACGAAAAGAACATGGTCATCATGCCCGGCTACTGCGTGCAG
NM_001256463.1      TTCCGGAAATGGGCCGGAAACGAAAAGAACATGGTCATCATGCCCGGCTACTGCGTGCAG
NM_017871.5         TTCCGGAAATGGGCCGGAAACGAAAAGAACATGGTCATCATGCCCGGCTACTGCGTGCAG
NM_001256460.1      TTCCGGAAATGGGCCGGAAACGAAAAGAACATGGTCATCATGCCCGGCTACTGCGTGCAG
NM_001256456.1      TTCCGGAAATGGGCCGGAAACGAAAAGAACATGGTCATCATGCCCGGCTACTGCGTGCAG
NM_001256462.1      TTCCGGAAATGGGCCGGAAACGAAAAGAACATGGTCATCATGCCCGGCTACTGCGTGCAG
                    ************************************************************

HsCD00511087        GGCACCGTCGGCCACAAGATCCTCAGCGGGCAGCGGAAGCTCGAGATGGAGGGGCGGCAG
NM_001256463.1      GGCACCGTCGGCCACAAGATCCTCAGCGGGCAGCGGAAGCTCGAGATGGAGGGGCGGCAG
NM_017871.5         GGCACCGTCGGCCACAAGATCCTCAGCGGGCAGCGGAAGCTCGAGATGGAGGGGCGGCAG
NM_001256460.1      GGCACCGTCGGCCACAAGATCCTCAGCGGGCAGCGGAAGCTCGAGATGGAGGGGCGGCAG
NM_001256456.1      GGCACCGTCGGCCACAAGATCCTCAGCGGGCAGCGGAAGCTCGAGATGGAGGGGCGGCAG
NM_001256462.1      GGCACCGTCGGCCACAAGATCCTCAGCGGGCAGCGGAAGCTCGAGATGGAGGGGCGGCAG
                    ************************************************************

HsCD00511087        GTGCTGGAGGTCAAGATGCAGGTGGAGTACATGTCATTCAGCGCACACGCGGACGCCAAG
NM_001256463.1      GTGCTGGAGGTCAAGATGCAGGTGGAGTACATGTCATTCAGCGCACACGCGGACGCCAAG
NM_017871.5         GTGCTGGAGGTCAAGATGCAGGTGGAGTACATGTCATTCAGCGCACACGCGGACGCCAAG
NM_001256460.1      GTGCTGGAGGTCAAGATGCAGGTGGAGTACATGTCATTCAGCGCACACGCGGACGCCAAG
NM_001256456.1      GTGCTGGAGGTCAAGATGCAGGTGGAGTACATGTCATTCAGCGCACACGCGGACGCCAAG
NM_001256462.1      GTGCTGGAGGTCAAGATGCAGGTGGAGTACATGTCATTCAGCGCACACGCGGACGCCAAG
                    ************************************************************

HsCD00511087        GGCATCATGCAGCTGGTG------------------------------------------
NM_001256463.1      GGCATCATGCAGCTGGTGGGCCAGGCAGAGCCGGAGAGCGTGCTGCTGGTGCATGGCGAG
NM_017871.5         GGCATCATGCAGCTGGTGGGCCAGGCAGAGCCGGAGAGCGTGCTGCTGGTGCATGGCGAG
NM_001256460.1      GGCATCATGCAGCTGGTGGGCCAGGCAGAGCCGGAGAGCGTGCTGCTGGTGCATGGCGAG
NM_001256456.1      GGCATCATGCAGCTGGTGGGCCAGGCAGAGCCGGAGAGCGTGCTGCTGGTGCATGGCGAG
NM_001256462.1      GGCATCATGCAGCTGGTGGGCCAGGCAGAGCCGGAGAGCGTGCTGCTGGTGCATGGCGAG
                    ******************                                          

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      GCCAAGAAGATGGAGTTCCTGAAGCAGAAGATCGAGCAGGAGCTCCGGGTCAACTGCTAC
NM_017871.5         GCCAAGAAGATGGAGTTCCTGAAGCAGAAGATCGAGCAGGAGCTCCGGGTCAACTGCTAC
NM_001256460.1      GCCAAGAAGATGGAGTTCCTGAAGCAGAAGATCGAGCAGGAGCTCCGGGTCAACTGCTAC
NM_001256456.1      GCCAAGAAGATGGAGTTCCTGAAGCAGAAGATCGAGCAGGAGCTCCGGGTCAACTGCTAC
NM_001256462.1      GCCAAGAAGATGGAGTTCCTGAAGCAGAAGATCGAGCAGGAGCTCCGGGTCAACTGCTAC
                                                                                

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      ATGCCGGCCAATGGCGAGACGGTGACGCTGCCCACAAGCCCCAGCATCCCCGTAGGCATC
NM_017871.5         ATGCCGGCCAATGGCGAGACGGTGACGCTGCCCACAAGCCCCAGCATCCCCGTAGGCATC
NM_001256460.1      ATGCCGGCCAATGGCGAGACGGTGACGCTGCCCACAAGCCCCAGCATCCCCGTAGGCATC
NM_001256456.1      ATGCCGGCCAATGGCGAGACGGTGACGCTGCCCACAAGCCCCAGCATCCCCGTAGGCATC
NM_001256462.1      ATGCCGGCCAATGGCGAGACGGTGACGCTGCCCACAAGCCCCAGCATCCCCGTAGGCATC
                                                                                

HsCD00511087        ------------------------------------------------------------
NM_001256463.1      TCGCTGGGGCTGCTGAAGCGGGAGATGGCGCAGGGGCTGCTCCCTGAGGCCAAGAAGCCT
NM_017871.5         TCGCTGGGGCTGCTGAAGCGGGAGATGGCGCAGGGGCTGCTCCCTGAGGCCAAGAAGCCT
NM_001256460.1      TCGCTGGGGCTGCTGAAGCGGGAGATGGCGCAGGGGCTGCTCCCTGAGGCCAAGAAGCCT
NM_001256456.1      TCGCTGGGGCTGCTGAAGCGGGAGATGGCGCAGGGGCTGCTCCCTGAGGCCAAGAAGCCT
NM_001256462.1      TCGCTGGGGCTGCTGAAGCGGGAGATGGCGCAGGGGCTGCTCCCTGAGGCCAAGAAGCCT
                                                                                

HsCD00511087        ---------------------------------------------------TCCTCAGAG
NM_001256463.1      CGGCTCCTGCACGGCACCCTGATCATGAAGGACAGCAACTTCCGGCTGGTGTCCTCAGAG
NM_017871.5         CGGCTCCTGCACGGCACCCTGATCATGAAGGACAGCAACTTCCGGCTGGTGTCCTCAGAG
NM_001256460.1      CGGCTCCTGCACGGCACCCTGATCATGAAGGACAGCAACTTCCGGCTGGTGTCCTCAGAG
NM_001256456.1      CGGCTCCTGCACGGCACCCTGATCATGAAGGACAGCAACTTCCGGCTGGTGTCCTCAGAG
NM_001256462.1      CGGCTCCTGCACGGCACCCTGATCATGAAGGACAGCAACTTCCGGCTGGTGTCCTCAGAG
                                                                       *********

HsCD00511087        CAAGCCCTCAAAGAGCTGGGTCTGGCTGAGCACCAGCTGCGCTTCACCTGCCGCGTGCAC
NM_001256463.1      CAAGCCCTCAAAGAGCTGGGTCTGGCTGAGCACCAGCTGCGCTTCACCTGCCGCGTGCAC
NM_017871.5         CAAGCCCTCAAAGAGCTGGGTCTGGCTGAGCACCAGCTGCGCTTCACCTGCCGCGTGCAC
NM_001256460.1      CAAGCCCTCAAAGAGCTGGGTCTGGCTGAGCACCAGCTGCGCTTCACCTGCCGCGTGCAC
NM_001256456.1      CAAGCCCTCAAAGAGCTGGGTCTGGCTGAGCACCAGCTGCGCTTCACCTGCCGCGTGCAC
NM_001256462.1      CAAGCCCTCAAAGAGCTGGGTCTGGCTGAGCACCAGCTGCGCTTCACCTGCCGCGTGCAC
                    ************************************************************

HsCD00511087        CTGCATGACACACGCAAGGAGCAGGAGACGGCATTGCGCGTCTACAGCCACCTCAAGAGC
NM_001256463.1      CTGCATGACACACGCAAGGAGCAGGAGACGGCATTGCGCGTCTACAGCCACCTCAAGAGC
NM_017871.5         CTGCATGACACACGCAAGGAGCAGGAGACGGCATTGCGCGTCTACAGCCACCTCAAGAGC
NM_001256460.1      CTGCATGACACACGCAAGGAGCAGGAGACGGCATTGCGCGTCTACAGCCACCTCAAGAGC
NM_001256456.1      CTGCATGACACACGCAAGGAGCAGGAGACGGCATTGCGCGTCTACAGCCACCTCAAGAGC
NM_001256462.1      CTGCATGACACACGCAAGGAGCAGGAGACGGCATTGCGCGTCTACAGCCACCTCAAGAGC
                    ************************************************************

HsCD00511087        GTCCTGAAGGACCACTGTGTGCAGCACCTCCCGGACGGCTCTGTGACTGTGGAGTCCGTC
NM_001256463.1      GTCCTGAAGGACCACTGTGTGCAGCACCTCCCAGACGGCTCTGTGACTGTGGAGTCCGTC
NM_017871.5         GTCCTGAAGGACCACTGTGTGCAGCACCTCCCAGACGGCTCTGTGACTGTGGAGTCCGTC
NM_001256460.1      GTCCTGAAGGACCACTGTGTGCAGCACCTCCCAGACGGCTCTGTGACTGTGGAGTCCGTC
NM_001256456.1      GTCCTGAAGGACCACTGTGTGCAGCACCTCCCAGACGGCTCTGTGACTGTGGAGTCCGTC
NM_001256462.1      GTCCTGAAGGACCACTGTGTGCAGCACCTCCCAGACGGCTCTGTGACTGTGGAGTCCGTC
                    ********************************.***************************

HsCD00511087        CTCCTCCAGGCCGCCGCCCCTTCTGAGGACCCAGGCACCAAGGTGCTGCTGGTCTCCTGG
NM_001256463.1      CTCCTCCAGGCCGCCGCCCCTTCTGAGGACCCAGGCACCAAGGTGCTGCTGGTCTCCTGG
NM_017871.5         CTCCTCCAGGCCGCCGCCCCTTCTGAGGACCCAGGCACCAAGGTGCTGCTGGTCTCCTGG
NM_001256460.1      CTCCTCCAGGCCGCCGCCCCTTCTGAGGACCCAGGCACCAAGGTGCTGCTGGTCTCCTGG
NM_001256456.1      CTCCTCCAGGCCGCCGCCCCTTCTGAGGACCCAGGCACCAAGGTGCTGCTGGTCTCCTGG
NM_001256462.1      CTCCTCCAGGCCGCCGCCCCTTCTGAGGACCCAGGCACCAAGGTGCTGCTGGTCTCCTGG
                    ************************************************************

HsCD00511087        ACCTACCAGGACGAGGAGCTGGGGAGCTTCCTCACATCTCTGCTGAAGAAGGGCCTCCCC
NM_001256463.1      ACCTACCAGGACGAGGAGCTGGGGAGCTTCCTCACATCTCTGCTGAAGAAGGGCCTCCCC
NM_017871.5         ACCTACCAGGACGAGGAGCTGGGGAGCTTCCTCACATCTCTGCTGAAGAAGGGCCTCCCC
NM_001256460.1      ACCTACCAGGACGAGGAGCTGGGGAGCTTCCTCACATCTCTGCTGAAGAAGGGCCTCCCC
NM_001256456.1      ACCTACCAGGACGAGGAGCTGGGGAGCTTCCTCACATCTCTGCTGAAGAAGGGCCTCCCC
NM_001256462.1      ACCTACCAGGACGAGGAGCTGGGGAGCTTCCTCACATCTCTGCTGAAGAAGGGCCTCCCC
                    ************************************************************

HsCD00511087        CAGGCCCCCAGC---
NM_001256463.1      CAGGCCCCCAGCTGA
NM_017871.5         CAGGCCCCCAGCTGA
NM_001256460.1      CAGGCCCCCAGCTGA
NM_001256456.1      CAGGCCCCCAGCTGA
NM_001256462.1      CAGGCCCCCAGCTGA
                    ************