DNASU Plasmid | TBC1D20 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TBC1D20

Gene Name: TBC1 domain family, member 20

Original Clone ID:

ccsbBroadEn_04845

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC014983.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511303

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1210nts

Open reading frame: 1 to 1210

Reference Sequence Alignment

ATGGCCCTCCGGAGTGCGCAGGGCGACGGCCCCACCTCCGGCCACTGGGACGGCGGCGCG
GAGAAGGCAGACTTTAACGCCAAAAGGAAAAAGAAAGTGGCAGAGATACACCAGGCTCTG
AACAGTGATCCCACTGATGTGGCTGCCCTTAGACGCATGGCTATCAGTGAAGGAGGGCTC
CTGACTGATGAGATCAGACGAAAAGTGTGGCCCAAGCTCCTCAATGTCAATGCCAATGAC
CCACCTCCTATATCAGGGAAGAACCTACGGCAGATGAGCAAGGACTACCAACAAGTGTTG
CTGGACGTCCGGCGGTCATTGCGGCGGTTCCCTCCTGGCATGCCAGAGGAACAGAGAGAA
GGGCTCCAGGAAGAACTGATTGACATCATCCTCCTCATCTTGGAGCGCAACCCTCAGCTG
CACTACTACCAGGGCTACCATGACATTGTGGTCACATTTCTGCTGGTGGTAGGCGAGAGG
CTGGCAACATCCCTGGTAGAAAAATTATCTACCCACCACCTCAGGGATTTTATGGATCCA
ACAATGGACAACACCAAGCATATATTAAACTATCTGATGCCCATCATTGACCAGGTGAAT
CCAGAGCTCCATGACTTCATGCAGAGTGCTGAGGTAGGGACCATCTTTGCCCTCAGCTGG
CTCATCACCTGGTTTGGGCATGTCCTGTCTGACTTCAGGCACGTCGTGCGGTTATATGAC
TTCTTCCTGGCCTGCCACCCACTGATGCCGATTTACTTTGCAGCCGTGATTGTGTTGTAT
CGCGAGCAGGAAGTCCTGGACTGTGACTGTGACATGGCCTCGGTCCACCACCTGTTGTCC
CAGATCCCTCAGGACTTGCCCTATGAGACACTGATCAGCAGAGCAGGAGACCTTTTTGTT
CAGTTTCCCCCATCCGAACTTGCTCGGGAGGCCGCTGCCCAACAGCAAGCTGAGAGGACG
GCAGCCTCTACTTTCAAAGACTTTGAGCTGGCATCAGCCCAGCAGAGGCCTGATATGGTG
CTGCGGCAGCGGTTTCGGGGACTTCTGCGGCCTGAAGATCGAACAAAAGATGTCCTGACC
AAGCCAAGGACCAACCGCTTTGTGAAATTGGCAGTGATGGGGCTGACAGTGGCACTTGGA
GCGGCTGCACTGGCTGTGGTGAAAAGTGCCCTGGAATGGGCCCCTAAGTTTCAGCTGCAG
CTGTTTCCC

Protein sequence predicted based on nucleotide sequence

MALRSAQGDGPTSGHWDGGAEKADFNAKRKKKVAEIHQALNSDPTDVAALRRMAISEGGL
LTDEIRRKVWPKLLNVNANDPPPISGKNLRQMSKDYQQVLLDVRRSLRRFPPGMPEEQRE
GLQEELIDIILLILERNPQLHYYQGYHDIVVTFLLVVGERLATSLVEKLSTHHLRDFMDP
TMDNTKHILNYLMPIIDQVNPELHDFMQSAEVGTIFALSWLITWFGHVLSDFRHVVRLYD
FFLACHPLMPIYFAAVIVLYREQEVLDCDCDMASVHHLLSQIPQDLPYETLISRAGDLFV
QFPPSELAREAAAQQQAERTAASTFKDFELASAQQRPDMVLRQRFRGLLRPEDRTKDVLT
KPRTNRFVKLAVMGLTVALGAAALAVVKSALEWAPKFQLQLFP

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

TBC1D20

Symbol Nomenclature :

TBC1D20

SYNONYM :

C20orf140

Designation :

TBC1 domain family member 20

Full Nomenclature :

TBC1 domain family, member 20

GENEID :

128637

Locus Tag :

RP5-852M4.5

HGNC :

16133

MIM :

611663

Vega :

OTTHUMG00000031637

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00511303        ATGGCCCTCCGGAGTGCGCAGGGCGACGGCCCCACCTCCGGCCACTGGGACGGCGGCGCG
NM_144628.2         ATGGCCCTCCGGAGTGCGCAGGGCGACGGCCCCACCTCCGGCCACTGGGACGGCGGCGCG
XM_005260661.1      ATGGCCCTCCGGAGTGCGCAGGGCGACGGCCCCACCTCCGGCCACTGGGACGGCGGCGCG
                    ************************************************************

HsCD00511303        GAGAAGGCAGACTTTAACGCCAAAAGGAAAAAGAAAGTGGCAGAGATACACCAGGCTCTG
NM_144628.2         GAGAAGGCAGACTTTAACGCCAAAAGGAAAAAGAAAGTGGCAGAGATACACCAGGCTCTG
XM_005260661.1      GAGAAGGCAGACTTTAACGCCAAAAGGAAAAAGAAAGTGGCAGAGATACACCAGGCTCTG
                    ************************************************************

HsCD00511303        AACAGTGATCCCACTGATGTGGCTGCCCTTAGACGCATGGCTATCAGTGAAGGAGGGCTC
NM_144628.2         AACAGTGATCCCACTGATGTGGCTGCCCTTAGACGCATGGCTATCAGTGAAGGAGGGCTC
XM_005260661.1      AACAGTGATCCCACTGATGTGGCTGCCCTTAGACGCATGGCTATCAGTGAAGGAGGGCTC
                    ************************************************************

HsCD00511303        CTGACTGATGAGATCAGACGAAAAGTGTGGCCCAAGCTCCTCAATGTCAATGCCAATGAC
NM_144628.2         CTGACTGATGAGATCAGACGAAAAGTGTGGCCCAAGCTCCTCAATGTCAATGCCAATGAC
XM_005260661.1      CTGACTGATGAGATCAGACGAAAAGTGTGGCCCAAGCTCCTCAATGTCAATGCCAATGAC
                    ************************************************************

HsCD00511303        CCACCTCCTATATCAGGGAAGAACCTACGGCAGATGAGCAAGGACTACCAACAAGTGTTG
NM_144628.2         CCACCTCCTATATCAGGGAAGAACCTACGGCAGATGAGCAAGGACTACCAACAAGTGTTG
XM_005260661.1      CCACCTCCTATATCAGGGAAGAACCTACGGCAGATGAGCAAGGACTACCAACAAGTGTTG
                    ************************************************************

HsCD00511303        CTGGACGTCCGGCGGTCATTGCGGCGGTTCCCTCCTGGCATGCCAGAGGAACAGAGAGAA
NM_144628.2         CTGGACGTCCGGCGGTCATTGCGGCGGTTCCCTCCTGGCATGCCAGAGGAACAGAGAGAA
XM_005260661.1      CTGGACGTCCGGCGGTCATTGCGGCGGTTCCCTCCTGGCATGCCAGAGGAACAGAGAGAA
                    ************************************************************

HsCD00511303        GGGCTCCAGGAAGAACTGATTGACATCATCCTCCTCATCTTGGAGCGCAACCCTCAGCTG
NM_144628.2         GGGCTCCAGGAAGAACTGATTGACATCATCCTCCTCATCTTGGAGCGCAACCCTCAGCTG
XM_005260661.1      GGGCTCCAGGAAGAACTGATTGACATCATCCTCCTCATCTTGGAGCGCAACCCTCAGCTG
                    ************************************************************

HsCD00511303        CACTACTACCAGGGCTACCATGACATTGTGGTCACATTTCTGCTGGTGGTAGGCGAGAGG
NM_144628.2         CACTACTACCAGGGCTACCATGACATTGTGGTCACATTTCTGCTGGTGGTAGGCGAGAGG
XM_005260661.1      CACTACTACCAGGGCTACCATGACATTGTGGTCACATTTCTGCTGGTGGTAGGCGAGAGG
                    ************************************************************

HsCD00511303        CTGGCAACATCCCTGGTAGAAAAATTATCTACCCACCACCTCAGGGATTTTATGGATCCA
NM_144628.2         CTGGCAACATCCCTGGTAGAAAAATTATCTACCCACCACCTCAGGGATTTTATGGATCCA
XM_005260661.1      CTGGCAACATCCCTGGTAGAAAAATTATCTACCCACCACCTCAGGGATTTTATGGATCCA
                    ************************************************************

HsCD00511303        ACAATGGACAACACCAAGCATATATTAAACTATCTGATGCCCATCATTGACCAGGTGAAT
NM_144628.2         ACAATGGACAACACCAAGCATATATTAAACTATCTGATGCCCATCATTGACCAGGTGAAT
XM_005260661.1      ACAATGGACAACACCAAGCATATATTAAACTATCTGATGCCCATCATTGACCAGGTGAAT
                    ************************************************************

HsCD00511303        CCAGAGCTCCATGACTTCATGCAGAGTGCTGAGGTAGGGACCATCTTTGCCCTCAGCTGG
NM_144628.2         CCAGAGCTCCATGACTTCATGCAGAGTGCTGAGGTAGGGACCATCTTTGCCCTCAGCTGG
XM_005260661.1      CCAGAGCTCCATGACTTCATGCAGAGTGCTGAGGTAGGGACCATCTTTGCCCTCAGCTGG
                    ************************************************************

HsCD00511303        CTCATCACCTGGTTTGGGCATGTCCTGTCTGACTTCAGGCACGTCGTGCGGTTATATGAC
NM_144628.2         CTCATCACCTGGTTTGGGCATGTCCTGTCTGACTTCAGGCACGTCGTGCGGTTATATGAC
XM_005260661.1      CTCATCACCTGGTTTGGGCATGTCCTGTCTGACTTCAGGCACGTCGTGCGGTTATATGAC
                    ************************************************************

HsCD00511303        TTCTTCCTGGCCTGCCACCCACTGATGCCGATTTACTTTGCAGCCGTGATTGTGTTGTAT
NM_144628.2         TTCTTCCTGGCCTGCCACCCACTGATGCCGATTTACTTTGCAGCCGTGATTGTGTTGTAT
XM_005260661.1      TTCTTCCTGGCCTGCCACCCACTGATGCCGATTTACTTTGCAGCCGTGATTGTGTTGTAT
                    ************************************************************

HsCD00511303        CGCGAGCAGGAAGTCCTGGACTGTGACTGTGACATGGCCTCGGTCCACCACCTGTTGTCC
NM_144628.2         CGCGAGCAGGAAGTCCTGGACTGTGACTGTGACATGGCCTCGGTCCACCACCTGTTGTCC
XM_005260661.1      CGCGAGCAGGAAGTCCTGGACTGTGACTGTGACATGGCCTCGGTCCACCACCTGTTGTCC
                    ************************************************************

HsCD00511303        CAGATCCCTCAGGACTTGCCCTATGAGACACTGATCAGCAGAGCAGGAGACCTTTTTGTT
NM_144628.2         CAGATCCCTCAGGACTTGCCCTATGAGACACTGATCAGCAGAGCAGGAGACCTTTTTGTT
XM_005260661.1      CAGATCCCTCAGGACTTGCCCTATGAGACACTGATCAGCAGAGCAGGAGACCTTTTTGTT
                    ************************************************************

HsCD00511303        CAGTTTCCCCCATCCGAACTTGCTCGGGAGGCCGCTGCCCAACAGCAAGCTGAGAGGACG
NM_144628.2         CAGTTTCCCCCATCCGAACTTGCTCGGGAGGCCGCTGCCCAACAGCAAGCTGAGAGGACG
XM_005260661.1      CAGTTTCCCCCATCCGAACTTGCTCGGGAGGCCGCTGCCCAACAGCAAGCTGAGAGGACG
                    ************************************************************

HsCD00511303        GCAGCCTCTACTTTCAAAGACTTTGAGCTGGCATCAGCCCAGCAGAGGCCTGATATGGTG
NM_144628.2         GCAGCCTCTACTTTCAAAGACTTTGAGCTGGCATCAGCCCAGCAGAGGCCTGATATGGTG
XM_005260661.1      GCAGCCTCTACTTTCAAAGACTTTGAGCTGGCATCAGCCCAGCAGAGGCCTGATATGGTG
                    ************************************************************

HsCD00511303        CTGCGGCAGCGGTTTCGGGGACTTCTGCGGCCTGAAGATCGAACAAAAGATGTCCTGACC
NM_144628.2         CTGCGGCAGCGGTTTCGGGGACTTCTGCGGCCTGAAGATCGAACAAAAGATGTCCTGACC
XM_005260661.1      CTGCGGCAGCGGTTTCGGGGACTTCTGCGGCCTGAAGATCGAACAAAAGATGTCCTGACC
                    ************************************************************

HsCD00511303        AAGCCAAGGACCAACCGCTTTGTGAAATTGGCAGTGATGGGGCTGACAGTGGCACTTGGA
NM_144628.2         AAGCCAAGGACCAACCGCTTTGTGAAATTGGCAGTGATGGGGCTGACAGTGGCACTTGGA
XM_005260661.1      AAGCCAAGGACCAACCGCTTTGTGAAATTGGCAGTGATGGGGCTGACAGTGGCACTTGGA
                    ************************************************************

HsCD00511303        GCGGCTGCACTGGCTGTGGTGAAAAGTGCCCTGGAATGGGCCCCTAAGTTTCAGCTGCAG
NM_144628.2         GCGGCTGCACTGGCTGTGGTGAAAAGTGCCCTGGAATGGGCCCCTAAGTTTCAGCTGCAG
XM_005260661.1      GCGGCTGCACTGGCTGTGGTGAAAAGTGCCCTGGAATGGGCCCCTAAGTTTCAGCTGCAG
                    ************************************************************

HsCD00511303        CTGTTTCCC---
NM_144628.2         CTGTTTCCCTGA
XM_005260661.1      CTGTTTCCCTGA
                    *********