DNASU Plasmid | VSIG8 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: VSIG8

Gene Name: V-set and immunoglobulin domain containing 8

Original Clone ID:

ccsbBroadEn_05619

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC132893.1,BC132895.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511376

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1243nts

Open reading frame: 1 to 1243

Reference Sequence Alignment

ATGAGAGTTGGAGGAGCATTCCACCTTCTACTCGTGTGCCTGAGCCCAGCACTGCTGTCT
GCTGTGCGGATCAACGGGGATGGACAGGAGGTCCTGTACCTGGCAGAAGGTGATAATGTG
AGGCTGGGCTGCCCCTACGTCCTGGACCCTGAGGACTATGGTCCCAATGGGCTGGACATC
GAGTGGATGCAGGTCAACTCAGACCCCGCCCACCACCGAGAGAACGTGTTCCTTAGTTAC
CAGGACAAGAGGATCAACCATGGCAGCCTTCCCCATCTGCAGCAGAGGGTCCGCTTTGCA
GCCTCAGACCCAAGCCAGTACGATGCCTCCATCAACCTCATGAACCTGCAGGTATCTGAT
ACAGCCACTTATGAGTGCCGGGTGAAGAAGACCACCATGGCCACCCGGAAGGTCATTGTC
ACTGTCCAAGCACGACCTGCAGTGCCCATGTGCTGGACAGAGGGCCACATGACATATGGC
AACGATGTGGTGCTGAAGTGCTATGCCAGTGGGGGCTCCCAGCCCCTCTCCTACAAGTGG
GCCAAGATCAGTGGGCACCATTACCCCTATCGAGCTGGGTCTTACACCTCCCAGCACAGC
TACCACTCAGAGCTGTCCTACCAGGAGTCCTTCCACAGCTCCATAAACCAAGGCCTGAAC
AATGGGGACCTGGTGTTGAAGGATATCTCCAGAGCAGATGATGGGCTGTATCAGTGCACA
GTGGCCAACAACGTGGGCTACAGTGTTTGTGTGGTGGAGGTGAAGGTCTCAGACTCCCGG
CGTATAGGCGTGATCATCGGCATCGTCCTGGGCTCTCTGCTCGCGCTGGGCTGCCTGGCC
GTAGGCATCTGGGGGCTCGTCTGCTGCTGCTGCGGGGGCTCCGGGGCTGGCGGCGCCCGC
GGTGCCTTCGGCTACGGCAACGGCGGCGGGGTCGGCGGAGGGGCCTGCGGCGACTTGGCT
AGTGAGATCAGAGAGGACGCCGTGGCGCCCGGGTGCAAGGCCAGCGGGCGCGGCAGCCGC
GTCACCCACCTCCTGGGGTACCCGACGCAGAACGTCAGCCGCTCCCTGCGCCGCAAGTAC
GCGCCTCCCCCCTGCGGCGGCCCCGAGGACGTGGCCCTGGCGCCCTGCACCGCCGCCGCC
GCCTGCGAAGCGGGCCCCTCCCCGGTCTACGTCAAGGTCAAGAGCGCGGAGCCGGCTGAC
TGCGCCGAGGGGCCGGTGCAGTGCAAGAACGGCCTCTTGGTG

Protein sequence predicted based on nucleotide sequence

MRVGGAFHLLLVCLSPALLSAVRINGDGQEVLYLAEGDNVRLGCPYVLDPEDYGPNGLDI
EWMQVNSDPAHHRENVFLSYQDKRINHGSLPHLQQRVRFAASDPSQYDASINLMNLQVSD
TATYECRVKKTTMATRKVIVTVQARPAVPMCWTEGHMTYGNDVVLKCYASGGSQPLSYKW
AKISGHHYPYRAGSYTSQHSYHSELSYQESFHSSINQGLNNGDLVLKDISRADDGLYQCT
VANNVGYSVCVVEVKVSDSRRIGVIIGIVLGSLLALGCLAVGIWGLVCCCCGGSGAGGAR
GAFGYGNGGGVGGGACGDLASEIREDAVAPGCKASGRGSRVTHLLGYPTQNVSRSLRRKY
APPPCGGPEDVALAPCTAAAACEAGPSPVYVKVKSAEPADCAEGPVQCKNGLLV

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

VSIG8

Symbol Nomenclature :

VSIG8

Designation :

V-set and immunoglobulin domain-containing protein 8

Full Nomenclature :

V-set and immunoglobulin domain containing 8

GENEID :

391123

Locus Tag :

RP11-190A12.5

GenBank Accession :

HQ258612

HGNC :

32063

Vega :

OTTHUMG00000168834

Target GenBank:

HQ258612

Reference Sequence Alignment

  

HsCD00511376        ATGAGAGTTGGAGGAGCATTCCACCTTCTACTCGTGTGCCTGAGCCCAGCACTGCTGTCT
NM_001013661.1      ATGAGAGTTGGAGGAGCATTCCACCTTCTACTCGTGTGCCTGAGCCCAGCACTGCTGTCT
                    ************************************************************

HsCD00511376        GCTGTGCGGATCAACGGGGATGGACAGGAGGTCCTGTACCTGGCAGAAGGTGATAATGTG
NM_001013661.1      GCTGTGCGGATCAACGGGGATGGACAGGAGGTCCTGTACCTGGCAGAAGGTGATAATGTG
                    ************************************************************

HsCD00511376        AGGCTGGGCTGCCCCTACGTCCTGGACCCTGAGGACTATGGTCCCAATGGGCTGGACATC
NM_001013661.1      AGGCTGGGCTGCCCCTACGTCCTGGACCCTGAGGACTATGGTCCCAATGGGCTGGACATC
                    ************************************************************

HsCD00511376        GAGTGGATGCAGGTCAACTCAGACCCCGCCCACCACCGAGAGAACGTGTTCCTTAGTTAC
NM_001013661.1      GAGTGGATGCAGGTCAACTCAGACCCCGCCCACCACCGAGAGAACGTGTTCCTTAGTTAC
                    ************************************************************

HsCD00511376        CAGGACAAGAGGATCAACCATGGCAGCCTTCCCCATCTGCAGCAGAGGGTCCGCTTTGCA
NM_001013661.1      CAGGACAAGAGGATCAACCATGGCAGCCTTCCCCATCTGCAGCAGAGGGTCCGCTTTGCA
                    ************************************************************

HsCD00511376        GCCTCAGACCCAAGCCAGTACGATGCCTCCATCAACCTCATGAACCTGCAGGTATCTGAT
NM_001013661.1      GCCTCAGACCCAAGCCAGTACGATGCCTCCATCAACCTCATGAACCTGCAGGTATCTGAT
                    ************************************************************

HsCD00511376        ACAGCCACTTATGAGTGCCGGGTGAAGAAGACCACCATGGCCACCCGGAAGGTCATTGTC
NM_001013661.1      ACAGCCACTTATGAGTGCCGGGTGAAGAAGACCACCATGGCCACCCGGAAGGTCATTGTC
                    ************************************************************

HsCD00511376        ACTGTCCAAGCACGACCTGCAGTGCCCATGTGCTGGACAGAGGGCCACATGACATATGGC
NM_001013661.1      ACTGTCCAAGCACGACCTGCAGTGCCCATGTGCTGGACAGAGGGCCACATGACATATGGC
                    ************************************************************

HsCD00511376        AACGATGTGGTGCTGAAGTGCTATGCCAGTGGGGGCTCCCAGCCCCTCTCCTACAAGTGG
NM_001013661.1      AACGATGTGGTGCTGAAGTGCTATGCCAGTGGGGGCTCCCAGCCCCTCTCCTACAAGTGG
                    ************************************************************

HsCD00511376        GCCAAGATCAGTGGGCACCATTACCCCTATCGAGCTGGGTCTTACACCTCCCAGCACAGC
NM_001013661.1      GCCAAGATCAGTGGGCACCATTACCCCTATCGAGCTGGGTCTTACACCTCCCAGCACAGC
                    ************************************************************

HsCD00511376        TACCACTCAGAGCTGTCCTACCAGGAGTCCTTCCACAGCTCCATAAACCAAGGCCTGAAC
NM_001013661.1      TACCACTCAGAGCTGTCCTACCAGGAGTCCTTCCACAGCTCCATAAACCAAGGCCTGAAC
                    ************************************************************

HsCD00511376        AATGGGGACCTGGTGTTGAAGGATATCTCCAGAGCAGATGATGGGCTGTATCAGTGCACA
NM_001013661.1      AATGGGGACCTGGTGTTGAAGGATATCTCCAGAGCAGATGATGGGCTGTATCAGTGCACA
                    ************************************************************

HsCD00511376        GTGGCCAACAACGTGGGCTACAGTGTTTGTGTGGTGGAGGTGAAGGTCTCAGACTCCCGG
NM_001013661.1      GTGGCCAACAACGTGGGCTACAGTGTTTGTGTGGTGGAGGTGAAGGTCTCAGACTCCCGG
                    ************************************************************

HsCD00511376        CGTATAGGCGTGATCATCGGCATCGTCCTGGGCTCTCTGCTCGCGCTGGGCTGCCTGGCC
NM_001013661.1      CGTATAGGCGTGATCATCGGCATCGTCCTGGGCTCTCTGCTCGCGCTGGGCTGCCTGGCC
                    ************************************************************

HsCD00511376        GTAGGCATCTGGGGGCTCGTCTGCTGCTGCTGCGGGGGCTCCGGGGCTGGCGGCGCCCGC
NM_001013661.1      GTAGGCATCTGGGGGCTCGTCTGCTGCTGCTGCGGGGGCTCCGGGGCTGGCGGCGCCCGC
                    ************************************************************

HsCD00511376        GGTGCCTTCGGCTACGGCAACGGCGGCGGGGTCGGCGGAGGGGCCTGCGGCGACTTGGCT
NM_001013661.1      GGTGCCTTCGGCTACGGCAACGGCGGCGGGGTCGGCGGAGGGGCCTGCGGCGACTTGGCT
                    ************************************************************

HsCD00511376        AGTGAGATCAGAGAGGACGCCGTGGCGCCCGGGTGCAAGGCCAGCGGGCGCGGCAGCCGC
NM_001013661.1      AGTGAGATCAGAGAGGACGCCGTGGCGCCCGGGTGCAAGGCCAGCGGGCGCGGCAGCCGC
                    ************************************************************

HsCD00511376        GTCACCCACCTCCTGGGGTACCCGACGCAGAACGTCAGCCGCTCCCTGCGCCGCAAGTAC
NM_001013661.1      GTCACCCACCTCCTGGGGTACCCGACGCAGAACGTCAGCCGCTCCCTGCGCCGCAAGTAC
                    ************************************************************

HsCD00511376        GCGCCTCCCCCCTGCGGCGGCCCCGAGGACGTGGCCCTGGCGCCCTGCACCGCCGCCGCC
NM_001013661.1      GCGCCTCCCCCCTGCGGCGGCCCCGAGGACGTGGCCCTGGCGCCCTGCACCGCCGCCGCC
                    ************************************************************

HsCD00511376        GCCTGCGAAGCGGGCCCCTCCCCGGTCTACGTCAAGGTCAAGAGCGCGGAGCCGGCTGAC
NM_001013661.1      GCCTGCGAAGCGGGCCCCTCCCCGGTCTACGTCAAGGTCAAGAGCGCGGAGCCGGCTGAC
                    ************************************************************

HsCD00511376        TGCGCCGAGGGGCCGGTGCAGTGCAAGAACGGCCTCTTGGTG---
NM_001013661.1      TGCGCCGAGGGGCCGGTGCAGTGCAAGAACGGCCTCTTGGTGTGA
                    ******************************************