DNASU Plasmid | VPS4A (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: VPS4A

Gene Name: vacuolar protein sorting 4 homolog A (S. cerevisiae)

Original Clone ID:

ccsbBroadEn_03003

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC047932.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511497

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1312nts

Open reading frame: 1 to 1312

Reference Sequence Alignment

ATGACAACGTCAACCCTCCAGAAAGCCATTGATCTGGTGACGAAAGCCACAGAGGAGGAC
AAAGCCAAGAACTACGAGGAGGCGCTGCGGCTGTACCAGCATGCGGTGGAGTACTTCCTC
CACGCTATCAAGTATGAGGCCCACAGCGACAAGGCCAAGGAGAGCATTCGAGCCAAGTGC
GTGCAGTACCTAGACCGGGCCGAGAAGCTGAAGGATTATTTACGAAGCAAAGAGAAACAC
GGCAAGAAGCCAGTCAAAGAGAACCAGAGTGAGGGCAAGGGCAGTGACAGTGACAGTGAA
GGGGATAATCCGGAGAAAAAGAAACTGCAAGAACAGCTGATGGGTGCCGTCGTGATGGAG
AAGCCCAACATACGGTGGAACGACGTGGCCGGGCTGGAGGGGGCCAAGGAGGCCCTCAAA
GAAGCTGTCATTTTGCCAATCAAATTCCCACACTTGTTCACAGGCAAGCGCACCCCCTGG
CGGGGGATTCTGCTGTTCGGACCCCCTGGCACAGGGAAATCCTACCTGGCCAAAGCCGTG
GCAACAGAGGCCAACAACTCCACCTTCTTCTCTGTGTCCTCCTCAGATCTGATGTCCAAG
TGGCTGGGGGAGAGTGAAAAGCTGGTCAAGAACCTGTTTGAGCTGGCCAGGCAGCACAAG
CCCTCCATCATCTTCATCGATGAGGTGGATTCCCTCTGCGGGTCCCGAAATGAAAATGAG
AGTGAGGCCGCCCGGAGGATCAAAACGGAGTTCTTGGTCCAGATGCAGGGGGTGGGGAAT
AACAATGATGGGACTCTGGTTCTTGGAGCCACAAACATCCCATGGGTGTTGGATTCGGCC
ATCAGGAGGAGGTTTGAAAAACGAATTTATATCCCCTTGCCGGAGGAAGCTGCCCGCGCC
CAGATGTTCCGGTTGCATCTCGGGAGCACTCCCCACAACCTCACGGATGCAAACATCCAC
GAGCTGGCCCGGAAGACGGAAGGCTACTCGGGCGCGGACATCAGCATCATCGTGCGGGAC
TCTCTCATGCAGCCCGTGAGGAAGGTGCAGTCGGCCACACACTTCAAAAAGGTCTGTGGC
CCCTCTCGCACCAACCCCAGCATGATGATTGATGACCTCCTGACTCCATGCTCACCAGGG
GACCCAGGAGCCATGGAGATGACTTGGATGGATGTCCCTGGGGACAAACTCTTAGAGCCT
GTGGTTTGCATGTCGGACATGCTGCGGTCTCTGGCCACCACCCGGCCCACGGTGAATGCA
GACGACCTCCTGAAAGTGAAGAAATTCTCAGAGGACTTTGGGCAAGAGAGT

Protein sequence predicted based on nucleotide sequence

MTTSTLQKAIDLVTKATEEDKAKNYEEALRLYQHAVEYFLHAIKYEAHSDKAKESIRAKC
VQYLDRAEKLKDYLRSKEKHGKKPVKENQSEGKGSDSDSEGDNPEKKKLQEQLMGAVVME
KPNIRWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAV
ATEANNSTFFSVSSSDLMSKWLGESEKLVKNLFELARQHKPSIIFIDEVDSLCGSRNENE
SEAARRIKTEFLVQMQGVGNNNDGTLVLGATNIPWVLDSAIRRRFEKRIYIPLPEEAARA
QMFRLHLGSTPHNLTDANIHELARKTEGYSGADISIIVRDSLMQPVRKVQSATHFKKVCG
PSRTNPSMMIDDLLTPCSPGDPGAMEMTWMDVPGDKLLEPVVCMSDMLRSLATTRPTVNA
DDLLKVKKFSEDFGQES

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

VPS4A

Symbol Nomenclature :

VPS4A

SYNONYM :

SKD1; SKD1A; SKD2; VPS4; VPS4-1

Designation :

SKD1-homolog|hVPS4|vacuolar protein sorting factor 4A|vacuolar protein sorting-associated protein 4A|vacuolar sorting protein 4

Full Nomenclature :

vacuolar protein sorting 4 homolog A (S. cerevisiae)

GENEID :

27183

GenBank Accession :

EU831747

HGNC :

13488

MIM :

609982

Vega :

OTTHUMG00000175589

Target GenBank:

EU831747

Reference Sequence Alignment

  

HsCD00511497      ATGACAACGTCAACCCTCCAGAAAGCCATTGATCTGGTGACGAAAGCCACAGAGGAGGAC
NM_013245.2       ATGACAACGTCAACCCTCCAGAAAGCCATTGATCTGGTGACGAAAGCCACAGAGGAGGAC
                  ************************************************************

HsCD00511497      AAAGCCAAGAACTACGAGGAGGCGCTGCGGCTGTACCAGCATGCGGTGGAGTACTTCCTC
NM_013245.2       AAAGCCAAGAACTACGAGGAGGCGCTGCGGCTGTACCAGCATGCGGTGGAGTACTTCCTC
                  ************************************************************

HsCD00511497      CACGCTATCAAGTATGAGGCCCACAGCGACAAGGCCAAGGAGAGCATTCGAGCCAAGTGC
NM_013245.2       CACGCTATCAAGTATGAGGCCCACAGCGACAAGGCCAAGGAGAGCATTCGAGCCAAGTGC
                  ************************************************************

HsCD00511497      GTGCAGTACCTAGACCGGGCCGAGAAGCTGAAGGATTATTTACGAAGCAAAGAGAAACAC
NM_013245.2       GTGCAGTACCTAGACCGGGCCGAGAAGCTGAAGGATTATTTACGAAGCAAAGAGAAACAC
                  ************************************************************

HsCD00511497      GGCAAGAAGCCAGTCAAAGAGAACCAGAGTGAGGGCAAGGGCAGTGACAGTGACAGTGAA
NM_013245.2       GGCAAGAAGCCAGTCAAAGAGAACCAGAGTGAGGGCAAGGGCAGTGACAGTGACAGTGAA
                  ************************************************************

HsCD00511497      GGGGATAATCCGGAGAAAAAGAAACTGCAAGAACAGCTGATGGGTGCCGTCGTGATGGAG
NM_013245.2       GGGGATAATCCGGAGAAAAAGAAACTGCAAGAACAGCTGATGGGTGCCGTCGTGATGGAG
                  ************************************************************

HsCD00511497      AAGCCCAACATACGGTGGAACGACGTGGCCGGGCTGGAGGGGGCCAAGGAGGCCCTCAAA
NM_013245.2       AAGCCCAACATACGGTGGAACGACGTGGCCGGGCTGGAGGGGGCCAAGGAGGCCCTCAAA
                  ************************************************************

HsCD00511497      GAAGCTGTCATTTTGCCAATCAAATTCCCACACTTGTTCACAGGCAAGCGCACCCCCTGG
NM_013245.2       GAAGCTGTCATTTTGCCAATCAAATTCCCACACTTGTTCACAGGCAAGCGCACCCCCTGG
                  ************************************************************

HsCD00511497      CGGGGGATTCTGCTGTTCGGACCCCCTGGCACAGGGAAATCCTACCTGGCCAAAGCCGTG
NM_013245.2       CGGGGGATTCTGCTGTTCGGACCCCCTGGCACAGGGAAATCCTACCTGGCCAAAGCCGTG
                  ************************************************************

HsCD00511497      GCAACAGAGGCCAACAACTCCACCTTCTTCTCTGTGTCCTCCTCAGATCTGATGTCCAAG
NM_013245.2       GCAACAGAGGCCAACAACTCCACCTTCTTCTCTGTGTCCTCCTCAGATCTGATGTCCAAG
                  ************************************************************

HsCD00511497      TGGCTGGGGGAGAGTGAAAAGCTGGTCAAGAACCTGTTTGAGCTGGCCAGGCAGCACAAG
NM_013245.2       TGGCTGGGGGAGAGTGAAAAGCTGGTCAAGAACCTGTTTGAGCTGGCCAGGCAGCACAAG
                  ************************************************************

HsCD00511497      CCCTCCATCATCTTCATCGATGAGGTGGATTCCCTCTGCGGGTCCCGAAATGAAAATGAG
NM_013245.2       CCCTCCATCATCTTCATCGATGAGGTGGATTCCCTCTGCGGGTCCCGAAATGAAAATGAG
                  ************************************************************

HsCD00511497      AGTGAGGCCGCCCGGAGGATCAAAACGGAGTTCTTGGTCCAGATGCAGGGGGTGGGGAAT
NM_013245.2       AGTGAGGCCGCCCGGAGGATCAAAACGGAGTTCTTGGTCCAGATGCAGGGGGTGGGGAAT
                  ************************************************************

HsCD00511497      AACAATGATGGGACTCTGGTTCTTGGAGCCACAAACATCCCATGGGTGTTGGATTCGGCC
NM_013245.2       AACAATGATGGGACTCTGGTTCTTGGAGCCACAAACATCCCATGGGTGTTGGATTCGGCC
                  ************************************************************

HsCD00511497      ATCAGGAGGAGGTTTGAAAAACGAATTTATATCCCCTTGCCGGAGGAAGCTGCCCGCGCC
NM_013245.2       ATCAGGAGGAGGTTTGAAAAACGAATTTATATCCCCTTGCCGGAGGAAGCTGCCCGCGCC
                  ************************************************************

HsCD00511497      CAGATGTTCCGGTTGCATCTCGGGAGCACTCCCCACAACCTCACGGATGCAAACATCCAC
NM_013245.2       CAGATGTTCCGGTTGCATCTCGGGAGCACTCCCCACAACCTCACGGATGCAAACATCCAC
                  ************************************************************

HsCD00511497      GAGCTGGCCCGGAAGACGGAAGGCTACTCGGGCGCGGACATCAGCATCATCGTGCGGGAC
NM_013245.2       GAGCTGGCCCGGAAGACGGAAGGCTACTCGGGCGCGGACATCAGCATCATCGTGCGGGAC
                  ************************************************************

HsCD00511497      TCTCTCATGCAGCCCGTGAGGAAGGTGCAGTCGGCCACACACTTCAAAAAGGTCTGTGGC
NM_013245.2       TCTCTCATGCAGCCCGTGAGGAAGGTGCAGTCGGCCACACACTTCAAAAAGGTCTGTGGC
                  ************************************************************

HsCD00511497      CCCTCTCGCACCAACCCCAGCATGATGATTGATGACCTCCTGACTCCATGCTCACCAGGG
NM_013245.2       CCCTCTCGCACCAACCCCAGCATGATGATTGATGACCTCCTGACTCCATGCTCACCAGGG
                  ************************************************************

HsCD00511497      GACCCAGGAGCCATGGAGATGACTTGGATGGATGTCCCTGGGGACAAACTCTTAGAGCCT
NM_013245.2       GACCCAGGAGCCATGGAGATGACTTGGATGGATGTCCCTGGGGACAAACTCTTAGAGCCT
                  ************************************************************

HsCD00511497      GTGGTTTGCATGTCGGACATGCTGCGGTCTCTGGCCACCACCCGGCCCACGGTGAATGCA
NM_013245.2       GTGGTTTGCATGTCGGACATGCTGCGGTCTCTGGCCACCACCCGGCCCACGGTGAATGCA
                  ************************************************************

HsCD00511497      GACGACCTCCTGAAAGTGAAGAAATTCTCAGAGGACTTTGGGCAAGAGAGT---
NM_013245.2       GACGACCTCCTGAAAGTGAAGAAATTCTCAGAGGACTTTGGGCAAGAGAGTTAA
                  ***************************************************

Pathways

NCI :

CXCR4-mediated signaling events

Reactome :

Budding and maturation of HIV virion

Reactome :

Disease

Reactome :

Endosomal Sorting Complex Required For Transport (ESCRT)

Reactome :

HIV Infection

Reactome :

HIV Life Cycle

Reactome :

Late Phase of HIV Life Cycle

Reactome :

Membrane Trafficking

Protein

SMART domain :

AAA : ATPases associated with a variety of cellular activities

SMART domain :

MIT : Microtubule Interacting and Trafficking molecule domain

HPRD :

11676

Clone

Cloning Information :

669445

Original Clone ID :

ccsbBroadEn_03003

Organism

Species Specific ID:

27183

Genome

Chromosome :

Map Location :

16q22.1

Ensembl :

ENSG00000132612

Reagents

Labome :

VPS4A-antibody