DNASU Plasmid | SLC2A4 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: SLC2A4

Gene Name: solute carrier family 2 (facilitated glucose transporter), member 4

Original Clone ID:

ccsbBroadEn_01542

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC069615.1,BC069621.1

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511521

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1528nts

Open reading frame: 1 to 1528

Reference Sequence Alignment

ATGCCGTCGGGCTTCCAACAGATAGGCTCCGAAGATGGGGAACCCCCTCAGCAGCGAGTG
ACTGGGACCCTGGTCCTTGCTGTGTTCTCTGCGGTGCTTGGCTCCCTGCAGTTTGGGTAC
AACATTGGGGTCATCAATGCCCCTCAGAAGGTGATTGAACAGAGCTACAATGAGACGTGG
CTGGGGAGGCAGGGGCCTGAGGGACCCAGCTCCATCCCTCCAGGCACCCTCACCACCCTC
TGGGCCCTCTCCGTGGCCATCTTTTCCGTGGGCGGCATGATTTCCTCCTTCCTCATTGGT
ATCATCTCTCAGTGGCTTGGAAGGAAAAGGGCCATGCTGGTCAACAATGTCCTGGCGGTG
CTGGGGGGCAGCCTCATGGGCCTGGCCAATGCTGCTGCCTCCTATGAAATGCTCATCCTT
GGACGATTCCTCATTGGCGCCTACTCAGGGCTGACATCAGGGCTGGTGCCCATGTACGTG
GGGGAGATTGCTCCCACTCACCTGCGGGGCGCCCTGGGGACGCTCAACCAACTGGCCATT
GTTATCGGCATTCTGATCGCCCAGGTGCTGGGCTTGGAGTCCCTCCTGGGCACTGCCAGC
CTGTGGCCACTGCTCCTGGGCCTCACAGTGCTACCTGCCCTCCTGCAGCTGGTCCTGCTG
CCCTTCTGTCCCGAGAGCCCCCGCTACCTCTACATCATCCAGAATCTCGAGGGGCCTGCC
AGAAAGAGTCTGAAGCGCCTGACAGGCTGGGCCGATGTTTCTGGAGTGCTGGCTGAGCTG
AAGGATGAGAAGCGGAAGCTGGAGCGTGAGCGGCCACTGTCCCTGCTCCAGCTCCTGGGC
AGCCGTACCCACCGGCAGCCCCTGATCATTGCGGTCGTGCTGCAGCTGAGCCAGCAGCTC
TCTGGCATCAATGCTGTTTTCTATTATTCGACCAGCATCTTCGAGACAGCAGGGGTAGGC
CAGCCTGCCTATGCCACCATAGGAGCTGGTGTGGTCAACACAGTCTTCACCTTGGTCTCG
GTGTTGTTGGTGGAGCGGGCGGGGCGCCGGACGCTCCATCTCCTGGGCCTGGCGGGCATG
TGTGGCTGTGCCATCCTGATGACTGTGGCTCTGCTCCTGCTGGAGCGAGTTCCAGCCATG
AGCTACGTCTCCATTGTGGCCATCTTTGGCTTCGTGGCATTTTTTGAGATTGGCCCTGGC
CCCATTCCTTGGTTCATCGTGGCCGAGCTCTTCAGCCAGGGACCCCGCCCGGCAGCCATG
GCTGTGGCTGGTTTCTCCAACTGGACGAGCAACTTCATCATTGGCATGGGTTTCCAGTAT
GTTGCGGAGGCTATGGGGCCCTACGTCTTCCTTCTATTTGCGGTCCTCCTGCTGGGCTTC
TTCATCTTCACCTTCTTAAGAGTACCTGAAACTCGAGGCCGGACGTTTGACCAGATCTCA
GCTGCCTTCCACCGGACACCCTCTCTTTTAGAGCAGGAGGTGAAACCCAGCACAGAACTT
GAGTATTTAGGGCCAGATGAGAACGAC

Protein sequence predicted based on nucleotide sequence

MPSGFQQIGSEDGEPPQQRVTGTLVLAVFSAVLGSLQFGYNIGVINAPQKVIEQSYNETW
LGRQGPEGPSSIPPGTLTTLWALSVAIFSVGGMISSFLIGIISQWLGRKRAMLVNNVLAV
LGGSLMGLANAAASYEMLILGRFLIGAYSGLTSGLVPMYVGEIAPTHLRGALGTLNQLAI
VIGILIAQVLGLESLLGTASLWPLLLGLTVLPALLQLVLLPFCPESPRYLYIIQNLEGPA
RKSLKRLTGWADVSGVLAELKDEKRKLERERPLSLLQLLGSRTHRQPLIIAVVLQLSQQL
SGINAVFYYSTSIFETAGVGQPAYATIGAGVVNTVFTLVSVLLVERAGRRTLHLLGLAGM
CGCAILMTVALLLLERVPAMSYVSIVAIFGFVAFFEIGPGPIPWFIVAELFSQGPRPAAM
AVAGFSNWTSNFIIGMGFQYVAEAMGPYVFLLFAVLLLGFFIFTFLRVPETRGRTFDQIS
AAFHRTPSLLEQEVKPSTELEYLGPDEND

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

1 error

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

SLC2A4

Symbol Nomenclature :

SLC2A4

SYNONYM :

GLUT4

Designation :

GLUT-4|glucose transporter type 4, insulin-responsive|insulin-responsive glucose transporter type 4|solute carrier family 2, facilitated glucose transporter member 4

Full Nomenclature :

solute carrier family 2 (facilitated glucose transporter), member 4

GENEID :

6517

GenBank Accession :

HQ258267

HGNC :

11009

MIM :

138190

Vega :

OTTHUMG00000102181

Target GenBank:

HQ258267

Reference Sequence Alignment

  

HsCD00511521      ATGCCGTCGGGCTTCCAACAGATAGGCTCCGAAGATGGGGAACCCCCTCAGCAGCGAGTG
NM_001042.2       ATGCCGTCGGGCTTCCAACAGATAGGCTCCGAAGATGGGGAACCCCCTCAGCAGCGAGTG
                  ************************************************************

HsCD00511521      ACTGGGACCCTGGTCCTTGCTGTGTTCTCTGCGGTGCTTGGCTCCCTGCAGTTTGGGTAC
NM_001042.2       ACTGGGACCCTGGTCCTTGCTGTGTTCTCTGCGGTGCTTGGCTCCCTGCAGTTTGGGTAC
                  ************************************************************

HsCD00511521      AACATTGGGGTCATCAATGCCCCTCAGAAGGTGATTGAACAGAGCTACAATGAGACGTGG
NM_001042.2       AACATTGGGGTCATCAATGCCCCTCAGAAGGTGATTGAACAGAGCTACAATGAGACGTGG
                  ************************************************************

HsCD00511521      CTGGGGAGGCAGGGGCCTGAGGGACCCAGCTCCATCCCTCCAGGCACCCTCACCACCCTC
NM_001042.2       CTGGGGAGGCAGGGGCCTGAGGGACCCAGCTCCATCCCTCCAGGCACCCTCACCACCCTC
                  ************************************************************

HsCD00511521      TGGGCCCTCTCCGTGGCCATCTTTTCCGTGGGCGGCATGATTTCCTCCTTCCTCATTGGT
NM_001042.2       TGGGCCCTCTCCGTGGCCATCTTTTCCGTGGGCGGCATGATTTCCTCCTTCCTCATTGGT
                  ************************************************************

HsCD00511521      ATCATCTCTCAGTGGCTTGGAAGGAAAAGGGCCATGCTGGTCAACAATGTCCTGGCGGTG
NM_001042.2       ATCATCTCTCAGTGGCTTGGAAGGAAAAGGGCCATGCTGGTCAACAATGTCCTGGCGGTG
                  ************************************************************

HsCD00511521      CTGGGGGGCAGCCTCATGGGCCTGGCCAATGCTGCTGCCTCCTATGAAATGCTCATCCTT
NM_001042.2       CTGGGGGGCAGCCTCATGGGCCTGGCCAATGCTGCTGCCTCCTATGAAATGCTCATCCTT
                  ************************************************************

HsCD00511521      GGACGATTCCTCATTGGCGCCTACTCAGGGCTGACATCAGGGCTGGTGCCCATGTACGTG
NM_001042.2       GGACGATTCCTCATTGGCGCCTACTCAGGGCTGACATCAGGGCTGGTGCCCATGTACGTG
                  ************************************************************

HsCD00511521      GGGGAGATTGCTCCCACTCACCTGCGGGGCGCCCTGGGGACGCTCAACCAACTGGCCATT
NM_001042.2       GGGGAGATTGCTCCCACTCACCTGCGGGGCGCCCTGGGGACGCTCAACCAACTGGCCATT
                  ************************************************************

HsCD00511521      GTTATCGGCATTCTGATCGCCCAGGTGCTGGGCTTGGAGTCCCTCCTGGGCACTGCCAGC
NM_001042.2       GTTATCGGCATTCTGATCGCCCAGGTGCTGGGCTTGGAGTCCCTCCTGGGCACTGCCAGC
                  ************************************************************

HsCD00511521      CTGTGGCCACTGCTCCTGGGCCTCACAGTGCTACCTGCCCTCCTGCAGCTGGTCCTGCTG
NM_001042.2       CTGTGGCCACTGCTCCTGGGCCTCACAGTGCTACCTGCCCTCCTGCAGCTGGTCCTGCTG
                  ************************************************************

HsCD00511521      CCCTTCTGTCCCGAGAGCCCCCGCTACCTCTACATCATCCAGAATCTCGAGGGGCCTGCC
NM_001042.2       CCCTTCTGTCCCGAGAGCCCCCGCTACCTCTACATCATCCAGAATCTCGAGGGGCCTGCC
                  ************************************************************

HsCD00511521      AGAAAGAGTCTGAAGCGCCTGACAGGCTGGGCCGATGTTTCTGGAGTGCTGGCTGAGCTG
NM_001042.2       AGAAAGAGTCTGAAGCGCCTGACAGGCTGGGCCGATGTTTCTGGAGTGCTGGCTGAGCTG
                  ************************************************************

HsCD00511521      AAGGATGAGAAGCGGAAGCTGGAGCGTGAGCGGCCACTGTCCCTGCTCCAGCTCCTGGGC
NM_001042.2       AAGGATGAGAAGCGGAAGCTGGAGCGTGAGCGGCCACTGTCCCTGCTCCAGCTCCTGGGC
                  ************************************************************

HsCD00511521      AGCCGTACCCACCGGCAGCCCCTGATCATTGCGGTCGTGCTGCAGCTGAGCCAGCAGCTC
NM_001042.2       AGCCGTACCCACCGGCAGCCCCTGATCATTGCGGTCGTGCTGCAGCTGAGCCAGCAGCTC
                  ************************************************************

HsCD00511521      TCTGGCATCAATGCTGTTTTCTATTATTCGACCAGCATCTTCGAGACAGCAGGGGTAGGC
NM_001042.2       TCTGGCATCAATGCTGTTTTCTATTATTCGACCAGCATCTTCGAGACAGCAGGGGTAGGC
                  ************************************************************

HsCD00511521      CAGCCTGCCTATGCCACCATAGGAGCTGGTGTGGTCAACACAGTCTTCACCTTGGTCTCG
NM_001042.2       CAGCCTGCCTATGCCACCATAGGAGCTGGTGTGGTCAACACAGTCTTCACCTTGGTCTCG
                  ************************************************************

HsCD00511521      GTGTTGTTGGTGGAGCGGGCGGGGCGCCGGACGCTCCATCTCCTGGGCCTGGCGGGCATG
NM_001042.2       GTGTTGTTGGTGGAGCGGGCGGGGCGCCGGACGCTCCATCTCCTGGGCCTGGCGGGCATG
                  ************************************************************

HsCD00511521      TGTGGCTGTGCCATCCTGATGACTGTGGCTCTGCTCCTGCTGGAGCGAGTTCCAGCCATG
NM_001042.2       TGTGGCTGTGCCATCCTGATGACTGTGGCTCTGCTCCTGCTGGAGCGAGTTCCAGCCATG
                  ************************************************************

HsCD00511521      AGCTACGTCTCCATTGTGGCCATCTTTGGCTTCGTGGCATTTTTTGAGATTGGCCCTGGC
NM_001042.2       AGCTACGTCTCCATTGTGGCCATCTTTGGCTTCGTGGCATTTTTTGAGATTGGCCCTGGC
                  ************************************************************

HsCD00511521      CCCATTCCTTGGTTCATCGTGGCCGAGCTCTTCAGCCAGGGACCCCGCCCGGCAGCCATG
NM_001042.2       CCCATTCCTTGGTTCATCGTGGCCGAGCTCTTCAGCCAGGGACCCCGCCCGGCAGCCATG
                  ************************************************************

HsCD00511521      GCTGTGGCTGGTTTCTCCAACTGGACGAGCAACTTCATCATTGGCATGGGTTTCCAGTAT
NM_001042.2       GCTGTGGCTGGTTTCTCCAACTGGACGAGCAACTTCATCATTGGCATGGGTTTCCAGTAT
                  ************************************************************

HsCD00511521      GTTGCGGAGGCTATGGGGCCCTACGTCTTCCTTCTATTTGCGGTCCTCCTGCTGGGCTTC
NM_001042.2       GTTGCGGAGGCTATGGGGCCCTACGTCTTCCTTCTATTTGCGGTCCTCCTGCTGGGCTTC
                  ************************************************************

HsCD00511521      TTCATCTTCACCTTCTTAAGAGTACCTGAAACTCGAGGCCGGACGTTTGACCAGATCTCA
NM_001042.2       TTCATCTTCACCTTCTTAAGAGTACCTGAAACTCGAGGCCGGACGTTTGACCAGATCTCA
                  ************************************************************

HsCD00511521      GCTGCCTTCCACCGGACACCCTCTCTTTTAGAGCAGGAGGTGAAACCCAGCACAGAACTT
NM_001042.2       GCTGCCTTCCACCGGACACCCTCTCTTTTAGAGCAGGAGGTGAAACCCAGCACAGAACTT
                  ************************************************************

HsCD00511521      GAGTATTTAGGGCCAGATGAGAACGAC---
NM_001042.2       GAGTATTTAGGGCCAGATGAGAACGACTGA
                  ***************************

Pathways

NCI :

Arf6 trafficking events

NCI :

Class I PI3K signaling events mediated by Akt

NCI :

Insulin-mediated glucose transport

Reactome :

Developmental Biology

Reactome :

Disease

Reactome :

Facilitative Na+-independent glucose transporters

Reactome :

Glucose transport

Reactome :

Glycogen storage diseases

Reactome :

Hexose transport

Reactome :

Membrane Trafficking

Reactome :

Metabolism

Reactome :

Metabolism of carbohydrates

Reactome :

Myoclonic epilepsy of Lafora

Reactome :

SLC-mediated transmembrane transport

Reactome :

Transcriptional regulation of white adipocyte differentiation

Reactome :

Translocation of GLUT4 to the plasma membrane

Reactome :

Transmembrane transport of small molecules

Reactome :

Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds

WikiPathway :

Adipogenesis

WikiPathway :

AMPK signaling

WikiPathway :

Glycolysis and Gluconeogenesis

WikiPathway :

Insulin Signaling

WikiPathway :

Type II diabetes mellitus

Protein

HPRD :

00688

Clone

Cloning Information :

611853

Original Clone ID :

ccsbBroadEn_01542

Organism

Species Specific ID:

6517

Genome

Chromosome :

Map Location :

17p13

Ensembl :

ENSG00000181856

Reagents

Labome :

GLUT4-antibody