DNASU Plasmid | TRIM31 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TRIM31

Gene Name: tripartite motif containing 31

Original Clone ID:

ccsbBroadEn_07742

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC017017.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511845

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1276nts

Open reading frame: 1 to 1276

Reference Sequence Alignment

ATGGCCAGTGGGCAGTTTGTGAACAAACTGCAAGAGGAAGTGATCTGCCCCATCTGCCTG
GACATTCTGCAGAAACCTGTCACCATCGACTGTGGGCACAATTTCTGCCTCAAATGCATC
ACTCAGATTGGGGAAACATCATGTGGATTTTTCAAATGTCCCCTCTGCAAAACTTCCGTA
AGGAAGAACGCAATCAGGTTCAACTCGCTGTTGCGGAATCTGGTGGAGAAAATCCAAGCT
CTACAAGCCTCTGAGGTGCAGTCCAAAAGGAAAGAGGCTACATGCCCGAGGCACCAGGAG
ATGTTCCACTATTTCTGCGAGGATGATGGGAAGTTCCTCTGTTTTGTGTGTCGTGAATCC
AAGGACCACAAATCCCATAATGTCAGCTTGATCGAAGAAGCTGCCCAGAATTATCAGGGG
CAGATTCAAGAGCAGATCCAAGTCTTGCAGCAAAAGGAGAAGGAGACAGTACAAGTGAAG
GCACAAGGTGTACACAGGGTCGATGTCTTCACGGACCAGGTAGAACATGAGAAGCAAAGG
ATCCTCACAGAATTTGAACTCCTGCATCAAGTCCTAGAGGAGGAGAAGAATTTCCTGCTA
TCACGGATTTACTGGCTGGGTCATGAGGGAACGGAAGCGGGGAAACACTATGTTGCCTCC
ACTGAGCCACAGTTGAACGATCTCAAGAAGCTCGTTGATTCCCTGAAGACCAAGCAGAAC
ATGCCACCCAGGCAGCTGCTGGAGGATATCAAAGTCGTCTTGTGCAGAAGTGAAGAGTTT
CAGTTTCTCAACCCAACCCCTGTTCCTCTGGAACTGGAGAAAAAACTCAGTGAAGCAAAA
TCAAGACACGACTCCATCACAGGGAGCCTGAAAAAATTCAAAGACCAACTCCAGGCTGAT
AGGAAAAAAGATGAAAACAGATTCTTCAAAAGCATGAATAAAAATGACATGAAGAGCTGG
GGCTTGTTACAGAAAAATAATCATAAAATGAACAAAACCTCAGAGCCCGGGTCATCTTCT
GCAGGCGGCAGAACTACATCGGGGCCACCAAATCACCACTCTTCAGCCCCATCCCACTCC
CTGTTTCGGGCCTCGTCTGCTGGGAAAGTCACTTTTCCAGTATGTCTCCTGGCCTCTTAT
GATGAGATTTCTGGTCAAGGAGCGAGCTCTCAGGATACGAAGACATTTGACGTTGCGCTG
TCCGAGGAGCTCCATGCGGCACTGAGTGAGTGGCTGACAGCGATCCGGGCTTGGTTTTGT
AAGGTTCCTTCAAGC

Protein sequence predicted based on nucleotide sequence

MASGQFVNKLQEEVICPICLDILQKPVTIDCGHNFCLKCITQIGETSCGFFKCPLCKTSV
RKNAIRFNSLLRNLVEKIQALQASEVQSKRKEATCPRHQEMFHYFCEDDGKFLCFVCRES
KDHKSHNVSLIEEAAQNYQGQIQEQIQVLQQKEKETVQVKAQGVHRVDVFTDQVEHEKQR
ILTEFELLHQVLEEEKNFLLSRIYWLGHEGTEAGKHYVASTEPQLNDLKKLVDSLKTKQN
MPPRQLLEDIKVVLCRSEEFQFLNPTPVPLELEKKLSEAKSRHDSITGSLKKFKDQLQAD
RKKDENRFFKSMNKNDMKSWGLLQKNNHKMNKTSEPGSSSAGGRTTSGPPNHHSSAPSHS
LFRASSAGKVTFPVCLLASYDEISGQGASSQDTKTFDVALSEELHAALSEWLTAIRAWFC
KVPSS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

TRIM31

Symbol Nomenclature :

TRIM31

SYNONYM :

C6orf13; HCG1; HCGI; RNF

Designation :

E3 ubiquitin-protein ligase TRIM31|ring finger protein|tripartite motif-containing 31 gamma|tripartite motif-containing protein 31

Full Nomenclature :

tripartite motif containing 31

GENEID :

11074

Locus Tag :

DAAP-112O13.2

GenBank Accession :

DQ894616

HGNC :

16289

MIM :

609316

Vega :

OTTHUMG00000031064

Target GenBank:

DQ894616

Reference Sequence Alignment

  

HsCD00511845      ATGGCCAGTGGGCAGTTTGTGAACAAACTGCAAGAGGAAGTGATCTGCCCCATCTGCCTG
NM_007028.3       ATGGCCAGTGGGCAGTTTGTGAACAAACTGCAAGAGGAAGTGATCTGCCCCATCTGCCTG
                  ************************************************************

HsCD00511845      GACATTCTGCAGAAACCTGTCACCATCGACTGTGGGCACAATTTCTGCCTCAAATGCATC
NM_007028.3       GACATTCTGCAGAAACCTGTCACCATCGACTGTGGGCACAATTTCTGCCTCAAATGCATC
                  ************************************************************

HsCD00511845      ACTCAGATTGGGGAAACATCATGTGGATTTTTCAAATGTCCCCTCTGCAAAACTTCCGTA
NM_007028.3       ACTCAGATTGGGGAAACATCATGTGGATTTTTCAAATGTCCCCTCTGCAAAACTTCCGTA
                  ************************************************************

HsCD00511845      AGGAAGAACGCAATCAGGTTCAACTCGCTGTTGCGGAATCTGGTGGAGAAAATCCAAGCT
NM_007028.3       AGGAAGAACGCAATCAGGTTCAACTCGCTGTTGCGGAATCTGGTGGAGAAAATCCAAGCT
                  ************************************************************

HsCD00511845      CTACAAGCCTCTGAGGTGCAGTCCAAAAGGAAAGAGGCTACATGCCCGAGGCACCAGGAG
NM_007028.3       CTACAAGCCTCTGAGGTGCAGTCCAAAAGGAAAGAGGCTACATGCCCGAGGCACCAGGAG
                  ************************************************************

HsCD00511845      ATGTTCCACTATTTCTGCGAGGATGATGGGAAGTTCCTCTGTTTTGTGTGTCGTGAATCC
NM_007028.3       ATGTTCCACTATTTCTGCGAGGATGATGGGAAGTTCCTCTGTTTTGTGTGTCGTGAATCC
                  ************************************************************

HsCD00511845      AAGGACCACAAATCCCATAATGTCAGCTTGATCGAAGAAGCTGCCCAGAATTATCAGGGG
NM_007028.3       AAGGACCACAAATCCCATAATGTCAGCTTGATCGAAGAAGCTGCCCAGAATTATCAGGGG
                  ************************************************************

HsCD00511845      CAGATTCAAGAGCAGATCCAAGTCTTGCAGCAAAAGGAGAAGGAGACAGTACAAGTGAAG
NM_007028.3       CAGATTCAAGAGCAGATCCAAGTCTTGCAGCAAAAGGAGAAGGAGACAGTACAAGTGAAG
                  ************************************************************

HsCD00511845      GCACAAGGTGTACACAGGGTCGATGTCTTCACGGACCAGGTAGAACATGAGAAGCAAAGG
NM_007028.3       GCACAAGGTGTACACAGGGTCGATGTCTTCACGGACCAGGTAGAACATGAGAAGCAAAGG
                  ************************************************************

HsCD00511845      ATCCTCACAGAATTTGAACTCCTGCATCAAGTCCTAGAGGAGGAGAAGAATTTCCTGCTA
NM_007028.3       ATCCTCACAGAATTTGAACTCCTGCATCAAGTCCTAGAGGAGGAGAAGAATTTCCTGCTA
                  ************************************************************

HsCD00511845      TCACGGATTTACTGGCTGGGTCATGAGGGAACGGAAGCGGGGAAACACTATGTTGCCTCC
NM_007028.3       TCACGGATTTACTGGCTGGGTCATGAGGGAACGGAAGCGGGGAAACACTATGTTGCCTCC
                  ************************************************************

HsCD00511845      ACTGAGCCACAGTTGAACGATCTCAAGAAGCTCGTTGATTCCCTGAAGACCAAGCAGAAC
NM_007028.3       ACTGAGCCACAGTTGAACGATCTCAAGAAGCTCGTTGATTCCCTGAAGACCAAGCAGAAC
                  ************************************************************

HsCD00511845      ATGCCACCCAGGCAGCTGCTGGAGGATATCAAAGTCGTCTTGTGCAGAAGTGAAGAGTTT
NM_007028.3       ATGCCACCCAGGCAGCTGCTGGAGGATATCAAAGTCGTCTTGTGCAGAAGTGAAGAGTTT
                  ************************************************************

HsCD00511845      CAGTTTCTCAACCCAACCCCTGTTCCTCTGGAACTGGAGAAAAAACTCAGTGAAGCAAAA
NM_007028.3       CAGTTTCTCAACCCAACCCCTGTTCCTCTGGAACTGGAGAAAAAACTCAGTGAAGCAAAA
                  ************************************************************

HsCD00511845      TCAAGACACGACTCCATCACAGGGAGCCTGAAAAAATTCAAAGACCAACTCCAGGCTGAT
NM_007028.3       TCAAGACATGACTCCATCACAGGGAGCCTAAAAAAATTCAAAGACCAACTCCAGGCTGAT
                  ******** ********************.******************************

HsCD00511845      AGGAAAAAAGATGAAAACAGATTCTTCAAAAGCATGAATAAAAATGACATGAAGAGCTGG
NM_007028.3       AGGAAAAAAGATGAAAACAGATTCTTCAAAAGCATGAATAAAAATGACATGAAGAGCTGG
                  ************************************************************

HsCD00511845      GGCTTGTTACAGAAAAATAATCATAAAATGAACAAAACCTCAGAGCCCGGGTCATCTTCT
NM_007028.3       GGCTTGTTACAGAAAAATAATCATAAAATGAACAAAACCTCAGAGCCCGGGTCATCTTCT
                  ************************************************************

HsCD00511845      GCAGGCGGCAGAACTACATCGGGGCCACCAAATCACCACTCTTCAGCCCCATCCCACTCC
NM_007028.3       GCAGGCGGCAGAACTACATCGGGGCCACCAAATCACCACTCTTCAGCCCCATCCCACTCC
                  ************************************************************

HsCD00511845      CTGTTTCGGGCCTCGTCTGCTGGGAAAGTCACTTTTCCAGTATGTCTCCTGGCCTCTTAT
NM_007028.3       CTGTTTCGGGCCTCGTCTGCTGGGAAAGTCACTTTTCCAGTATGTCTCCTGGCCTCTTAT
                  ************************************************************

HsCD00511845      GATGAGATTTCTGGTCAAGGAGCGAGCTCTCAGGATACGAAGACATTTGACGTTGCGCTG
NM_007028.3       GATGAGATTTCTGGTCAAGGAGCGAGCTCTCAGGATACGAAGACATTTGACGTTGCGCTG
                  ************************************************************

HsCD00511845      TCCGAGGAGCTCCATGCGGCACTGAGTGAGTGGCTGACAGCGATCCGGGCTTGGTTTTGT
NM_007028.3       TCCGAGGAGCTCCATGCGGCACTGAGTGAGTGGCTGACAGCGATCCGGGCTTGGTTTTGT
                  ************************************************************

HsCD00511845      AAGGTTCCTTCAAGC---
NM_007028.3       GAGGTTCCTTCAAGCTAA
                  .**************