DNASU Plasmid | AP1M2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: AP1M2

Gene Name: adaptor-related protein complex 1, mu 2 subunit

Original Clone ID:

ccsbBroadEn_11451

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC005021.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511850

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1276nts

Open reading frame: 1 to 1276

Reference Sequence Alignment

ATGTCCGCCTCGGCTGTCTTCATTCTGGACGTTAAGGGCAAGCCATTGATCAGCCGCAAC
TACAAGGGCGATGTGGCCATGAGCAAGATTGAGCACTTCATGCCTTTGCTGGTACAGCGG
GAGGAGGAAGGCGCCCTGGCCCCGCTGCTGAGCCACGGCCAGGTCCACTTCCTATGGATC
AAACACAGCAACCTCTACTTGGTGGCCACCACATCGAAGAATGCCAATGCCTCCCTGGTG
TACTCCTTCCTGTATAAGACAATAGAGGTATTCTGCGAATACTTCAAGGAGCTGGAGGAG
GAGAGCATCCGGGACAACTTTGTCATCGTCTACGAGTTGCTGGACGAGCTCATGGACTTT
GGCTTCCCGCAGACCACCGACAGCAAGATCCTGCAGGAGTACATCACTCAGCAGAGCAAC
AAGCTGGAGACGGGCAAGTCACGGGTGCCACCCACTGTCACCAACGCTGTGTCCTGGCGC
TCCGAGGGTATCAAGTATAAGAAGAACGAGGTCTTCATTGATGTCATAGAGTCTGTCAAC
CTGCTGGTCAATGCCAACGGCAGCGTCCTTCTGAGCGAAATCGTCGGTACCATCAAGCTC
AAGGTGTTTCTGTCGGGAATGCCAGAGCTGCGGCTGGGCCTCAATGACCGCGTGCTCTTC
GAGCTCACTGGCCTTTCAGGCAGCAAGAACAAATCAGTAGAGCTGGAGGATGTAAAATTC
CACCAGTGCGTGCGGCTCTCTCGCTTTGACAACGACCGCACCATCTCCTTCATCCCGCCT
GATGGTGACTTTGAGCTCATGTCATACCGCCTCAGCACCCAGGTCAAGCCACTGATCTGG
ATTGAGTCTGTCATTGAGAAGTTCTCCCACAGCCGCGTGGAGATCATGGTCAAGGCCAAG
GGGCAGTTTAAGAAACAGTCAGTGGCCAACGGTGTGGAGATATCTGTGCCTGTACCCAGC
GATGCCGACTCCCCCAGATTCAAGACCAGTGTGGGCAGCGCCAAGTATGTGCCGGAGAGA
AACGTCGTGATTTGGAGTATTAAGTCTTTCCCGGGGGGCAAGGAGTACTTGATGCGAGCC
CACTTTGGCCTCCCCCGTGTGGAAAAGGAAGAGGTGGAGGGCCGGCCCCCCATCGGGGTC
AAGTTTGAGATCCCCTACTTCACCGTCTCTGGGATCCAGGTCCGATACATGAAGATCATT
GAGAAAAGTGGTTACCAGGCCCTGCCCTGGGTTCGCTACATCACCCAGAGTGGCGATTAC
CAACTTCGTACCAGC

Protein sequence predicted based on nucleotide sequence

MSASAVFILDVKGKPLISRNYKGDVAMSKIEHFMPLLVQREEEGALAPLLSHGQVHFLWI
KHSNLYLVATTSKNANASLVYSFLYKTIEVFCEYFKELEEESIRDNFVIVYELLDELMDF
GFPQTTDSKILQEYITQQSNKLETGKSRVPPTVTNAVSWRSEGIKYKKNEVFIDVIESVN
LLVNANGSVLLSEIVGTIKLKVFLSGMPELRLGLNDRVLFELTGLSGSKNKSVELEDVKF
HQCVRLSRFDNDRTISFIPPDGDFELMSYRLSTQVKPLIWIESVIEKFSHSRVEIMVKAK
GQFKKQSVANGVEISVPVPSDADSPRFKTSVGSAKYVPERNVVIWSIKSFPGGKEYLMRA
HFGLPRVEKEEVEGRPPIGVKFEIPYFTVSGIQVRYMKIIEKSGYQALPWVRYITQSGDY
QLRTS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

AP1M2

Symbol Nomenclature :

AP1M2

SYNONYM :

AP1-mu2; HSMU1B; MU-1B; MU1B; mu2

Designation :

Full Nomenclature :

adaptor-related protein complex 1, mu 2 subunit

GENEID :

10053

GenBank Accession :

HQ447268

HGNC :

558

MIM :

607309

Vega :

OTTHUMG00000180584

Target GenBank:

HQ447268

Reference Sequence Alignment

  

HsCD00511850        ATGTCCGCCTCGGCTGTCTTCATTCTGGACGTTAAGGGCAAGCCATTGATCAGCCGCAAC
NM_005498.4         ATGTCCGCCTCGGCTGTCTTCATTCTGGACGTTAAGGGCAAGCCATTGATCAGCCGCAAC
XM_005259701.1      ATGTCCGCCTCGGCTGTCTTCATTCTGGACGTTAAGGGCAAGCCATTGATCAGCCGCAAC
                    ************************************************************

HsCD00511850        TACAAGGGCGATGTGGCCATGAGCAAGATTGAGCACTTCATGCCTTTGCTGGTACAGCGG
NM_005498.4         TACAAGGGCGATGTGGCCATGAGCAAGATTGAGCACTTCATGCCTTTGCTGGTACAGCGG
XM_005259701.1      TACAAGGGCGATGTGGCCATGAGCAAGATTGAGCACTTCATGCCTTTGCTGGTACAGCGG
                    ************************************************************

HsCD00511850        GAGGAGGAAGGCGCCCTGGCCCCGCTGCTGAGCCACGGCCAGGTCCACTTCCTATGGATC
NM_005498.4         GAGGAGGAAGGCGCCCTGGCCCCGCTGCTGAGCCACGGCCAGGTCCACTTCCTATGGATC
XM_005259701.1      GAGGAGGAAGGCGCCCTGGCCCCGCTGCTGAGCCACGGCCAGGTCCACTTCCTATGGATC
                    ************************************************************

HsCD00511850        AAACACAGCAACCTCTACTTGGTGGCCACCACATCGAAGAATGCCAATGCCTCCCTGGTG
NM_005498.4         AAACACAGCAACCTCTACTTGGTGGCCACCACATCGAAGAATGCCAATGCCTCCCTGGTG
XM_005259701.1      AAACACAGCAACCTCTACTTGGTGGCCACCACATCGAAGAATGCCAATGCCTCCCTGGTG
                    ************************************************************

HsCD00511850        TACTCCTTCCTGTATAAGACAATAGAGGTATTCTGCGAATACTTCAAGGAGCTGGAGGAG
NM_005498.4         TACTCCTTCCTGTATAAGACAATAGAGGTATTCTGCGAATACTTCAAGGAGCTGGAGGAG
XM_005259701.1      TACTCCTTCCTGTATAAGACAATAGAGGTATTCTGCGAATACTTCAAGGAGCTGGAGGAG
                    ************************************************************

HsCD00511850        GAGAGCATCCGGGACAACTTTGTCATCGTCTACGAGTTGCTGGACGAGCTCATGGACTTT
NM_005498.4         GAGAGCATCCGGGACAACTTTGTCATCGTCTACGAGTTGCTGGACGAGCTCATGGACTTT
XM_005259701.1      GAGAGCATCCGGGACAACTTTGTCATCGTCTACGAGTTGCTGGACGAGCTCATGGACTTT
                    ************************************************************

HsCD00511850        GGCTTCCCGCAGACCACCGACAGCAAGATCCTGCAGGAGTACATCACTCAGCAGAGCAAC
NM_005498.4         GGCTTCCCGCAGACCACCGACAGCAAGATCCTGCAGGAGTACATCACTCAGCAGAGCAAC
XM_005259701.1      GGCTTCCCGCAGACCACCGACAGCAAGATCCTGCAGGAGTACATCACTCAGCAGAGCAAC
                    ************************************************************

HsCD00511850        AAGCTGGAGACGGGCAAGTCACGGGTGCCACCCACTGTCACCAACGCTGTGTCCTGGCGC
NM_005498.4         AAGCTGGAGACGGGCAAGTCACGGGTGCCACCCACTGTCACCAACGCTGTGTCCTGGCGC
XM_005259701.1      AAGCTGGAGACGGGCAAGTCACGGGTGCCACCCACTGTCACCAACGCTGTGTCCTGGCGC
                    ************************************************************

HsCD00511850        TCCGAGGGTATCAAGTATAAGAAGAACGAGGTCTTCATTGATGTCATAGAGTCTGTCAAC
NM_005498.4         TCCGAGGGTATCAAGTATAAGAAGAACGAGGTCTTCATTGATGTCATAGAGTCTGTCAAC
XM_005259701.1      TCCGAGGGTATCAAGTATAAGAAGAACGAGGTCTTCATTGATGTCATAGAGTCTGTCAAC
                    ************************************************************

HsCD00511850        CTGCTGGTCAATGCCAACGGCAGCGTCCTTCTGAGCGAAATCGTCGGTACCATCAAGCTC
NM_005498.4         CTGCTGGTCAATGCCAACGGCAGCGTCCTTCTGAGCGAAATCGTCGGTACCATCAAGCTC
XM_005259701.1      CTGCTGGTCAATGCCAACGGCAGCGTCCTTCTGAGCGAAATCGTCGGTACCATCAAGCTC
                    ************************************************************

HsCD00511850        AAGGTGTTTCTGTCGGGAATGCCAGAGCTGCGGCTGGGCCTCAATGACCGCGTGCTCTTC
NM_005498.4         AAGGTGTTTCTGTCAGGAATGCCAGAGCTGCGGCTGGGCCTCAATGACCGCGTGCTCTTC
XM_005259701.1      AAGGTGTTTCTGTCAGGAATGCCAGAGCTGCGGCTGGGCCTCAATGACCGCGTGCTCTTC
                    **************.*********************************************

HsCD00511850        GAGCTCACTGGCCTTTCAGGCAGCAAGAACAAATCAGTAGAGCTGGAGGATGTAAAATTC
NM_005498.4         GAGCTCACTG------GCCGCAGCAAGAACAAATCAGTAGAGCTGGAGGATGTAAAATTC
XM_005259701.1      GAGCTCACTGGCCTTTCAGGCAGCAAGAACAAATCAGTAGAGCTGGAGGATGTAAAATTC
                    **********       . *****************************************

HsCD00511850        CACCAGTGCGTGCGGCTCTCTCGCTTTGACAACGACCGCACCATCTCCTTCATCCCGCCT
NM_005498.4         CACCAGTGCGTGCGGCTCTCTCGCTTTGACAACGACCGCACCATCTCCTTCATCCCGCCT
XM_005259701.1      CACCAGTGCGTGCGGCTCTCTCGCTTTGACAACGACCGCACCATCTCCTTCATCCCGCCT
                    ************************************************************

HsCD00511850        GATGGTGACTTTGAGCTCATGTCATACCGCCTCAGCACCCAGGTCAAGCCACTGATCTGG
NM_005498.4         GATGGTGACTTTGAGCTCATGTCATACCGCCTCAGCACCCAGGTCAAGCCACTGATCTGG
XM_005259701.1      GATGGTGACTTTGAGCTCATGTCATACCGCCTCAGCACCCAGGTCAAGCCACTGATCTGG
                    ************************************************************

HsCD00511850        ATTGAGTCTGTCATTGAGAAGTTCTCCCACAGCCGCGTGGAGATCATGGTCAAGGCCAAG
NM_005498.4         ATTGAGTCTGTCATTGAGAAGTTCTCCCACAGCCGCGTGGAGATCATGGTCAAGGCCAAG
XM_005259701.1      ATTGAGTCTGTCATTGAGAAGTTCTCCCACAGCCGCGTGGAGATCATGGTCAAGGCCAAG
                    ************************************************************

HsCD00511850        GGGCAGTTTAAGAAACAGTCAGTGGCCAACGGTGTGGAGATATCTGTGCCTGTACCCAGC
NM_005498.4         GGGCAGTTTAAGAAACAGTCAGTGGCCAACGGTGTGGAGATATCTGTGCCTGTACCCAGC
XM_005259701.1      GGGCAGTTTAAGAAACAGTCAGTGGCCAACGGTGTGGAGATATCTGTGCCTGTACCCAGC
                    ************************************************************

HsCD00511850        GATGCCGACTCCCCCAGATTCAAGACCAGTGTGGGCAGCGCCAAGTATGTGCCGGAGAGA
NM_005498.4         GATGCCGACTCCCCCAGATTCAAGACCAGTGTGGGCAGCGCCAAGTATGTGCCGGAGAGA
XM_005259701.1      GATGCCGACTCCCCCAGATTCAAGACCAGTGTGGGCAGCGCCAAGTATGTGCCGGAGAGA
                    ************************************************************

HsCD00511850        AACGTCGTGATTTGGAGTATTAAGTCTTTCCCGGGGGGCAAGGAGTACTTGATGCGAGCC
NM_005498.4         AACGTCGTGATTTGGAGTATTAAGTCTTTCCCGGGGGGCAAGGAGTACTTGATGCGAGCC
XM_005259701.1      AACGTCGTGATTTGGAGTATTAAGTCTTTCCCGGGGGGCAAGGAGTACTTGATGCGAGCC
                    ************************************************************

HsCD00511850        CACTTTGGCCTCCCCCGTGTGGAAAAGGAAGAGGTGGAGGGCCGGCCCCCCATCGGGGTC
NM_005498.4         CACTTTGGCCTCCCCAGTGTGGAAAAGGAAGAGGTGGAGGGCCGGCCCCCCATCGGGGTC
XM_005259701.1      CACTTTGGCCTCCCCAGTGTGGAAAAGGAAGAGGTGGAGGGCCGGCCCCCCATCGGGGTC
                    ***************.********************************************

HsCD00511850        AAGTTTGAGATCCCCTACTTCACCGTCTCTGGGATCCAGGTCCGATACATGAAGATCATT
NM_005498.4         AAGTTTGAGATCCCCTACTTCACCGTCTCTGGGATCCAGGTCCGATACATGAAGATCATT
XM_005259701.1      AAGTTTGAGATCCCCTACTTCACCGTCTCTGGGATCCAGGTCCGATACATGAAGATCATT
                    ************************************************************

HsCD00511850        GAGAAAAGTGGTTACCAGGCCCTGCCCTGGGTTCGCTACATCACCCAGAGTGGCGATTAC
NM_005498.4         GAGAAAAGTGGTTACCAGGCCCTGCCCTGGGTTCGCTACATCACCCAGAGTGGCGATTAC
XM_005259701.1      GAGAAAAGTGGTTACCAGGCCCTGCCCTGGGTTCGCTACATCACCCAGAGTGGCGATTAC
                    ************************************************************

HsCD00511850        CAACTTCGTACCAGC---
NM_005498.4         CAACTTCGTACCAGCTAG
XM_005259701.1      CAACTTCGTACCAGCTAG
                    ***************

Pathways

Reactome :

Adaptive Immune System

Reactome :

Clathrin derived vesicle budding

Reactome :

Disease

Reactome :

Golgi Associated Vesicle Biogenesis

Reactome :

HIV Infection

Reactome :

Host Interactions of HIV factors

Reactome :

Immune System

Reactome :

Lysosome Vesicle Biogenesis

Reactome :

Membrane Trafficking

Reactome :

MHC class II antigen presentation

Reactome :

Nef mediated downregulation of MHC class I complex cell surface expression

Reactome :

Nef-mediates down modulation of cell surface receptors by recruiting them to clathrin adapters

Reactome :

The role of Nef in HIV-1 replication and disease pathogenesis

Reactome :

trans-Golgi Network Vesicle Budding

Protein

HPRD :

06296

Clone

Cloning Information :

605155

Original Clone ID :

ccsbBroadEn_11451

Organism

Species Specific ID:

10053

Genome

Chromosome :

Map Location :

19p13.2

Ensembl :

ENSG00000129354

Reagents

Labome :

AP1M2-antibody