DNASU Plasmid | GPSM1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: GPSM1

Gene Name: G-protein signaling modulator 1

Original Clone ID:

ccsbBroadEn_11807

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC009979.1,BC017353.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511929

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1303nts

Open reading frame: 1 to 1303

Reference Sequence Alignment

ATGGAGGCGTCCTGCCTAGAGCTGGCGCTGGAGGGCGAGCGTCTGTGCAAGGCGGGCGAC
TTCAAGACAGGCGTGGCCTTCTTTGAGGCTGCTGTGCAGGTGGGCACCGAGGACCTGAAG
ACACTGAGTGCCATCTACAGCCAGCTGGGCAACGCCTACTTCTACCTGAAGGAGCACGGC
CGGGCGCTGGAATACCACAAGCATGACCTCCTGCTGGCGCGGACCATCGGTGACCGCATG
GGGGAGGCCAAGGCCAGTGGAAACCTGGGAAACACACTCAAGGTCCTGGGGCGCTTCGAC
GAGGCTGCCGTCTGCTGCCAGCGGCATCTGAGCATCGCCCAAGAGCAGGGAGACAAGGTT
GGGGAGGCGAGGGCCCTCTACAACATCGGGAACGTGTACCACGCCAAAGGCAAGCAACTG
TCCTGGAACGCCGCAAACGCCACGCAGGACCCCGGGCACCTGCCGCCCGATGTCCGAGAG
ACCCTGTGCAAGGCCTCCGAGTTCTACGAGAGGAACCTGTCCCTGGTGAAGGAGCTGGGC
GACCGTGCGGCGCAGGGCAGGGCCTACGGCAACCTGGGCAACACCCACTATTTGTTGGGG
AACTTCACAGAGGCCACGACCTTCCACAAGGAGCGCCTGGCCATTGCTAAGGAGTTTGGA
GACAAGGCAGCCGAGAGGAGGGCCTACAGCAACCTGGGGAACGCCCACGTCTTCCTGGGG
CGCTTTGACGTGGCCGCCGAGTACTACAAGAAGACGCTGCAACTGTCTCGGCAGCTCAGG
GACCAGGCAGTGGAGGCGCAGGCCTGCTACAGTCTGGGCAACACCTACACGCTGCTGCAG
GACTACGAGCGCGCGGCCGAGTACCACCTGCGGCACCTGCTCATTGCCCAGGAGCTGGCC
GACAGAGTGGGCGAGGGCCGGGCGTGCTGGAGCCTGGGAAATGCCTACGTGTCCATGGGG
CGCCCAGCGCAGGCCCTGACCTTCGCCAAGAAGCACCTGCAGATCTCCCAGGAGATCGGG
GACCGCCATGGGGAGCTCACGGCCCGCATGAACGTGGCGCAGCTGCAGCTGGTGCTCGGC
CGCCTGACCAGCCCGGCAGCCTCAGAGAAGCCTGACCTGGCCGGCTATGAGGCCCAGGGT
GAGTTCCAGGGTTGTGGGGGGGTCTTGCTCCCCACAGGCACGGACCGCATCAGGAGCTGC
GGAGGGGTGGGATCGAGGCCAGGCCAGCATGGCGGAGGTGGCAGCCGCCAGAAAATGGCG
CCTACAAGCCAGTTCTTCTTGGCCTCAGGGACAGCACAGGCC

Protein sequence predicted based on nucleotide sequence

MEASCLELALEGERLCKAGDFKTGVAFFEAAVQVGTEDLKTLSAIYSQLGNAYFYLKEHG
RALEYHKHDLLLARTIGDRMGEAKASGNLGNTLKVLGRFDEAAVCCQRHLSIAQEQGDKV
GEARALYNIGNVYHAKGKQLSWNAANATQDPGHLPPDVRETLCKASEFYERNLSLVKELG
DRAAQGRAYGNLGNTHYLLGNFTEATTFHKERLAIAKEFGDKAAERRAYSNLGNAHVFLG
RFDVAAEYYKKTLQLSRQLRDQAVEAQACYSLGNTYTLLQDYERAAEYHLRHLLIAQELA
DRVGEGRACWSLGNAYVSMGRPAQALTFAKKHLQISQEIGDRHGELTARMNVAQLQLVLG
RLTSPAASEKPDLAGYEAQGEFQGCGGVLLPTGTDRIRSCGGVGSRPGQHGGGGSRQKMA
PTSQFFLASGTAQA

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

GPSM1

Symbol Nomenclature :

GPSM1

SYNONYM :

AGS3

Designation :

G-protein signalling modulator 1 (AGS3-like, C. elegans)|G-protein-signaling modulator 1|activator of G-protein signaling 3

Full Nomenclature :

G-protein signaling modulator 1

GENEID :

26086

Locus Tag :

RP13-270L15.1

HGNC :

17858

MIM :

609491

Vega :

OTTHUMG00000020930

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00511929      ------------------------------------------------------------
NM_015597.5       ATGGCGGGCCCGGCCCCGCCCGCGGCCGACGAGCTCCCGGGCCCGGCCGCCAGGCGCCTC
                                                                              

HsCD00511929      ---------ATGGAGGCGTCCTGCCTAGAGCTGGCGCTGGAGGGCGAGCGTCTGTGCAAG
NM_015597.5       TACTCCAGGATGGAGGCGTCCTGCCTAGAGCTGGCGCTGGAGGGCGAGCGTCTGTGCAAG
                           ***************************************************

HsCD00511929      GCGGGCGACTTCAAGACAGGCGTGGCCTTCTTTGAGGCTGCTGTGCAGGTGGGCACCGAG
NM_015597.5       GCGGGCGACTTCAAGACAGGCGTGGCCTTCTTTGAGGCTGCTGTGCAGGTGGGCACCGAG
                  ************************************************************

HsCD00511929      GACCTGAAGACACTGAGTGCCATCTACAGCCAGCTGGGCAACGCCTACTTCTACCTGAAG
NM_015597.5       GACCTGAAGACACTGAGTGCCATCTACAGCCAGCTGGGCAACGCCTACTTCTACCTGAAG
                  ************************************************************

HsCD00511929      GAGCACGGCCGGGCGCTGGAATACCACAAGCATGACCTCCTGCTGGCGCGGACCATCGGT
NM_015597.5       GAGCACGGCCGGGCGCTGGAATACCACAAGCATGACCTCCTGCTGGCGCGGACCATCGGT
                  ************************************************************

HsCD00511929      GACCGCATGGGGGAGGCCAAGGCCAGTGGAAACCTGGGAAACACACTCAAGGTCCTGGGG
NM_015597.5       GACCGCATGGGGGAGGCCAAGGCCAGTGGAAACCTGGGAAACACACTCAAGGTCCTGGGG
                  ************************************************************

HsCD00511929      CGCTTCGACGAGGCTGCCGTCTGCTGCCAGCGGCATCTGAGCATCGCCCAAGAGCAGGGA
NM_015597.5       CGCTTCGACGAGGCTGCCGTCTGCTGCCAGCGGCATCTGAGCATCGCCCAAGAGCAGGGA
                  ************************************************************

HsCD00511929      GACAAGGTTGGGGAGGCGAGGGCCCTCTACAACATCGGGAACGTGTACCACGCCAAAGGC
NM_015597.5       GACAAGGTTGGGGAGGCGAGGGCCCTCTACAACATCGGGAACGTGTACCACGCCAAAGGC
                  ************************************************************

HsCD00511929      AAGCAACTGTCCTGGAACGCCGCAAACGCCACGCAGGACCCCGGGCACCTGCCGCCCGAT
NM_015597.5       AAGCAACTGTCCTGGAACGCCGCAAACGCCACGCAGGACCCCGGGCACCTGCCGCCCGAT
                  ************************************************************

HsCD00511929      GTCCGAGAGACCCTGTGCAAGGCCTCCGAGTTCTACGAGAGGAACCTGTCCCTGGTGAAG
NM_015597.5       GTCCGAGAGACCCTGTGCAAGGCCTCCGAGTTCTACGAGAGGAACCTGTCCCTGGTGAAG
                  ************************************************************

HsCD00511929      GAGCTGGGCGACCGTGCGGCGCAGGGCAGGGCCTACGGCAACCTGGGCAACACCCACTAT
NM_015597.5       GAGCTGGGCGACCGTGCGGCGCAGGGCAGGGCCTACGGCAACCTGGGCAACACCCACTAT
                  ************************************************************

HsCD00511929      TTGTTGGGGAACTTCACAGAGGCCACGACCTTCCACAAGGAGCGCCTGGCCATTGCTAAG
NM_015597.5       TTGTTGGGGAACTTCACAGAGGCCACGACCTTCCACAAGGAGCGCCTGGCCATTGCTAAG
                  ************************************************************

HsCD00511929      GAGTTTGGAGACAAGGCAGCCGAGAGGAGGGCCTACAGCAACCTGGGGAACGCCCACGTC
NM_015597.5       GAGTTTGGAGACAAGGCAGCCGAGAGGAGGGCCTACAGCAACCTGGGGAACGCCCACGTC
                  ************************************************************

HsCD00511929      TTCCTGGGGCGCTTTGACGTGGCCGCCGAGTACTACAAGAAGACGCTGCAACTGTCTCGG
NM_015597.5       TTCCTGGGGCGCTTTGACGTGGCCGCCGAGTACTACAAGAAGACGCTGCAACTGTCTCGG
                  ************************************************************

HsCD00511929      CAGCTCAGGGACCAGGCAGTGGAGGCGCAGGCCTGCTACAGTCTGGGCAACACCTACACG
NM_015597.5       CAGCTCAGGGACCAGGCAGTGGAGGCGCAGGCCTGCTACAGTCTGGGCAACACCTACACG
                  ************************************************************

HsCD00511929      CTGCTGCAGGACTACGAGCGCGCGGCCGAGTACCACCTGCGGCACCTGCTCATTGCCCAG
NM_015597.5       CTGCTGCAGGACTACGAGCGCGCGGCCGAGTACCACCTGCGGCACCTGCTCATTGCCCAG
                  ************************************************************

HsCD00511929      GAGCTGGCCGACAGAGTGGGCGAGGGCCGGGCGTGCTGGAGCCTGGGAAATGCCTACGTG
NM_015597.5       GAGCTGGCCGACAGAGTGGGCGAGGGCCGGGCGTGCTGGAGCCTGGGAAATGCCTACGTG
                  ************************************************************

HsCD00511929      TCCATGGGGCGCCCAGCGCAGGCCCTGACCTTCGCCAAGAAGCACCTGCAGATCTCCCAG
NM_015597.5       TCCATGGGGCGCCCAGCGCAGGCCCTGACCTTCGCCAAGAAGCACCTGCAGATCTCCCAG
                  ************************************************************

HsCD00511929      GAGATCGGGGACCGCCATGGGGAGCTCACGGCCCGCATGAACGTGGCGCAGCTGCAGCTG
NM_015597.5       GAGATCGGGGACCGCCATGGGGAGCTCACGGCCCGCATGAACGTGGCGCAGCTGCAGCTG
                  ************************************************************

HsCD00511929      GTGCTCGGCCGCCTGACCAGCCCGGCAGCCTCAGAGAAGCCTGACCTGGCCGGCTATGAG
NM_015597.5       GTGCTCGGCCGCCTGACCAGCCCGGCAGCCTCAGAGAAGCCTGACCTGGCCGGCTATGAG
                  ************************************************************

HsCD00511929      GCCCAGGGTGAGTTCCAGGGTTGTGGGGGGGTCTTGCTCCCCACAGGCACGGACCGCATC
NM_015597.5       GCCCAGGGTGAGTTCCAGGGTTGTGGGGGGGTCTTGCTCCCCACAGGCACGGACCGCATC
                  ************************************************************

HsCD00511929      AGGAGCTGCGGAGGGGTGGGATCGAGGCCAGGCCAGCATGGCGGAGGTGGCAGCCGCCAG
NM_015597.5       AGGAGCTGCGGAGGGGTGGGATCGAGGCCAGGCCAGCATGGCGGAGGTGGCAGCCGCCAG
                  ************************************************************

HsCD00511929      AAAATGGCGCCTACAAGCCAGTTCTTCTTGGCCTCAGGGACAGCACAGGCC---
NM_015597.5       AAAATGGCGCCTACAAGCCAGTTCTTCTTGGCCTCAGGGACAGCACAGGCCTGA
                  ***************************************************