DNASU Plasmid | CPA6 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: CPA6

Gene Name: carboxypeptidase A6

Original Clone ID:

ccsbBroadEn_08696

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC033684.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511951

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1312nts

Open reading frame: 1 to 1312

Reference Sequence Alignment

ATGAAGTGTCTCGGGAAGCGCAGGGGCCAGGCAGCTGCTTTCCTGCCTCTTTGCTGGCTC
TTTTTGAAGATTCTGCAACCGGGGCACAGCCACCTTTATAACAACCGCTATGCTGGTGAT
AAAGTGATAAGATTTATTCCCAAAACAGAAGAGGAAGCATATGCACTGAAGAAAATATCC
TATCAACTTAAGGTGGACCTGTGGCAGCCCAGCAGTATCTCCTATGTATCAGAGGGAACA
GTTACTGATGTCCATATCCCCCAAAATGGTTCCCGAGCCCTGTTAGCCTTCTTACAGGAA
GCCAACATCCAGTACAAGGTCCTCATAGAAGATCTTCAGAAAACACTGGAGAAGGGAAGC
AGCTTGCACACCCAGAGAAACCGAAGATCCCTCTCTGGATATAATTATGAAGTTTATCAC
TCCTTAGAAGAAATTCAAAATTGGATGCATCATCTGAATAAAACTCACTCAGGCCTCATT
CACATGTTCTCTATTGGAAGATCATATGAGGGAAGATGTCTTTTTATTTTAAAGCTGGGC
AGACGATCACGACTCAAAAGAGCTGTTTGGATAGACTGTGGTATTCATGCAAGAGAATGG
ATTGGTCCTGCCTTTTGTCAGTGGTTTGTAAAAGAAGCTCTTCTAACATATAAGAGTGAC
CCAGCCATGAGAAAAATGTTGAATCATCTATATTTCTATATCATGCCTGTGTTTAACGTC
GATGGATACCATTTTAGTTGGACCAATGATCGATTTTGGAGAAAAACAAGGTCAAGGAAC
TCAAGGTTTCGCTGCCGTGGAGTGGATGCCAATAGAAACTGGAAAGTGAAGTGGTGTGAT
GAAGGAGCTTCTATGCACCCTTGTGATGACACATACTGTGGCCCTTTTCCAGAATCTGAG
CCGGAAGTGAAGGCTGTAGCTAACTTCCTTCGAAAACACAGAAAGCACATTAGGGCTTAT
CTCTCCTTTCATGCATATGCTCAGATGTTACTGTATCCCTATTCTTACAAATATGCAACA
ATTCCCAATTTTAGATGTGTGGAATCTGCAGCTTATAAAGCTGTGAATGCACTTCAGTCA
GTATACGGGGTACGATACAGATATGGACCAGCCTCCACAACGTTGTATGTGAGCTCTGGT
AGCTCAATGGATTGGGCCTACAAAAATGGAATACCTTATGCATTTGCTTTCGAACTACGT
GACACTGGATATTTTGGATTTTTACTCCCAGAGATGCTCATCAAACCCACCTGTACAGAA
ACTATGCTGGCTGTGAAAAATATCACAATGCACCTGCTAAAGAAATGTCCC

Protein sequence predicted based on nucleotide sequence

MKCLGKRRGQAAAFLPLCWLFLKILQPGHSHLYNNRYAGDKVIRFIPKTEEEAYALKKIS
YQLKVDLWQPSSISYVSEGTVTDVHIPQNGSRALLAFLQEANIQYKVLIEDLQKTLEKGS
SLHTQRNRRSLSGYNYEVYHSLEEIQNWMHHLNKTHSGLIHMFSIGRSYEGRCLFILKLG
RRSRLKRAVWIDCGIHAREWIGPAFCQWFVKEALLTYKSDPAMRKMLNHLYFYIMPVFNV
DGYHFSWTNDRFWRKTRSRNSRFRCRGVDANRNWKVKWCDEGASMHPCDDTYCGPFPESE
PEVKAVANFLRKHRKHIRAYLSFHAYAQMLLYPYSYKYATIPNFRCVESAAYKAVNALQS
VYGVRYRYGPASTTLYVSSGSSMDWAYKNGIPYAFAFELRDTGYFGFLLPEMLIKPTCTE
TMLAVKNITMHLLKKCP

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

CPA6

Symbol Nomenclature :

CPA6

SYNONYM :

CPAH; ETL5; FEB11

Designation :

carboxypeptidase B

Full Nomenclature :

carboxypeptidase A6

GENEID :

57094

GenBank Accession :

DQ895237

HGNC :

17245

MIM :

609562

Vega :

OTTHUMG00000164575

Target GenBank:

DQ895237

Reference Sequence Alignment

  

HsCD00511951      ATGAAGTGTCTCGGGAAGCGCAGGGGCCAGGCAGCTGCTTTCCTGCCTCTTTGCTGGCTC
NM_020361.4       ATGAAGTGTCTCGGGAAGCGCAGGGGCCAGGCAGCTGCTTTCCTGCCTCTTTGCTGGCTC
                  ************************************************************

HsCD00511951      TTTTTGAAGATTCTGCAACCGGGGCACAGCCACCTTTATAACAACCGCTATGCTGGTGAT
NM_020361.4       TTTTTGAAGATTCTGCAACCGGGGCACAGCCACCTTTATAACAACCGCTATGCTGGTGAT
                  ************************************************************

HsCD00511951      AAAGTGATAAGATTTATTCCCAAAACAGAAGAGGAAGCATATGCACTGAAGAAAATATCC
NM_020361.4       AAAGTGATAAGATTTATTCCCAAAACAGAAGAGGAAGCATATGCACTGAAGAAAATATCC
                  ************************************************************

HsCD00511951      TATCAACTTAAGGTGGACCTGTGGCAGCCCAGCAGTATCTCCTATGTATCAGAGGGAACA
NM_020361.4       TATCAACTTAAGGTGGACCTGTGGCAGCCCAGCAGTATCTCCTATGTATCAGAGGGAACA
                  ************************************************************

HsCD00511951      GTTACTGATGTCCATATCCCCCAAAATGGTTCCCGAGCCCTGTTAGCCTTCTTACAGGAA
NM_020361.4       GTTACTGATGTCCATATCCCCCAAAATGGTTCCCGAGCCCTGTTAGCCTTCTTACAGGAA
                  ************************************************************

HsCD00511951      GCCAACATCCAGTACAAGGTCCTCATAGAAGATCTTCAGAAAACACTGGAGAAGGGAAGC
NM_020361.4       GCCAACATCCAGTACAAGGTCCTCATAGAAGATCTTCAGAAAACACTGGAGAAGGGAAGC
                  ************************************************************

HsCD00511951      AGCTTGCACACCCAGAGAAACCGAAGATCCCTCTCTGGATATAATTATGAAGTTTATCAC
NM_020361.4       AGCTTGCACACCCAGAGAAACCGAAGATCCCTCTCTGGATATAATTATGAAGTTTATCAC
                  ************************************************************

HsCD00511951      TCCTTAGAAGAAATTCAAAATTGGATGCATCATCTGAATAAAACTCACTCAGGCCTCATT
NM_020361.4       TCCTTAGAAGAAATTCAAAATTGGATGCATCATCTGAATAAAACTCACTCAGGCCTCATT
                  ************************************************************

HsCD00511951      CACATGTTCTCTATTGGAAGATCATATGAGGGAAGATGTCTTTTTATTTTAAAGCTGGGC
NM_020361.4       CACATGTTCTCTATTGGAAGATCATATGAGGGAAGATCTCTTTTTATTTTAAAGCTGGGC
                  ************************************* **********************

HsCD00511951      AGACGATCACGACTCAAAAGAGCTGTTTGGATAGACTGTGGTATTCATGCAAGAGAATGG
NM_020361.4       AGACGATCACGACTCAAAAGAGCTGTTTGGATAGACTGTGGTATTCATGCAAGAGAATGG
                  ************************************************************

HsCD00511951      ATTGGTCCTGCCTTTTGTCAGTGGTTTGTAAAAGAAGCTCTTCTAACATATAAGAGTGAC
NM_020361.4       ATTGGTCCTGCCTTTTGTCAGTGGTTTGTAAAAGAAGCTCTTCTAACATATAAGAGTGAC
                  ************************************************************

HsCD00511951      CCAGCCATGAGAAAAATGTTGAATCATCTATATTTCTATATCATGCCTGTGTTTAACGTC
NM_020361.4       CCAGCCATGAGAAAAATGTTGAATCATCTATATTTCTATATCATGCCTGTGTTTAACGTC
                  ************************************************************

HsCD00511951      GATGGATACCATTTTAGTTGGACCAATGATCGATTTTGGAGAAAAACAAGGTCAAGGAAC
NM_020361.4       GATGGATACCATTTTAGTTGGACCAATGATCGATTTTGGAGAAAAACAAGGTCAAGGAAC
                  ************************************************************

HsCD00511951      TCAAGGTTTCGCTGCCGTGGAGTGGATGCCAATAGAAACTGGAAAGTGAAGTGGTGTGAT
NM_020361.4       TCAAGGTTTCGCTGCCGTGGAGTGGATGCCAATAGAAACTGGAAAGTGAAGTGGTGTGAT
                  ************************************************************

HsCD00511951      GAAGGAGCTTCTATGCACCCTTGTGATGACACATACTGTGGCCCTTTTCCAGAATCTGAG
NM_020361.4       GAAGGAGCTTCTATGCACCCTTGTGATGACACATACTGTGGCCCTTTTCCAGAATCTGAG
                  ************************************************************

HsCD00511951      CCGGAAGTGAAGGCTGTAGCTAACTTCCTTCGAAAACACAGAAAGCACATTAGGGCTTAT
NM_020361.4       CCGGAAGTGAAGGCTGTAGCTAACTTCCTTCGAAAACACAGAAAGCACATTAGGGCTTAT
                  ************************************************************

HsCD00511951      CTCTCCTTTCATGCATATGCTCAGATGTTACTGTATCCCTATTCTTACAAATATGCAACA
NM_020361.4       CTCTCCTTTCATGCATATGCTCAGATGTTACTGTATCCCTATTCTTACAAATATGCAACA
                  ************************************************************

HsCD00511951      ATTCCCAATTTTAGATGTGTGGAATCTGCAGCTTATAAAGCTGTGAATGCACTTCAGTCA
NM_020361.4       ATTCCCAATTTTAGATGTGTGGAATCTGCAGCTTATAAAGCTGTGAATGCACTTCAGTCA
                  ************************************************************

HsCD00511951      GTATACGGGGTACGATACAGATATGGACCAGCCTCCACAACGTTGTATGTGAGCTCTGGT
NM_020361.4       GTATACGGGGTACGATACAGATATGGACCAGCCTCCACAACGTTGTATGTGAGCTCTGGT
                  ************************************************************

HsCD00511951      AGCTCAATGGATTGGGCCTACAAAAATGGAATACCTTATGCATTTGCTTTCGAACTACGT
NM_020361.4       AGCTCAATGGATTGGGCCTACAAAAATGGAATACCTTATGCATTTGCTTTCGAACTACGT
                  ************************************************************

HsCD00511951      GACACTGGATATTTTGGATTTTTACTCCCAGAGATGCTCATCAAACCCACCTGTACAGAA
NM_020361.4       GACACTGGATATTTTGGATTTTTACTCCCAGAGATGCTCATCAAACCCACCTGTACAGAA
                  ************************************************************

HsCD00511951      ACTATGCTGGCTGTGAAAAATATCACAATGCACCTGCTAAAGAAATGTCCC---
NM_020361.4       ACTATGCTGGCTGTGAAAAATATCACAATGCACCTGCTAAAGAAATGTCCCTGA
                  ***************************************************