DNASU Plasmid | FBXO5 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: FBXO5

Gene Name: F-box protein 5

Original Clone ID:

ccsbBroadEn_08010

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC018905.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511981

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1342nts

Open reading frame: 1 to 1342

Reference Sequence Alignment

ATGAGCCGGCGCCCCTGCAGCTGCGCCCTACGGCCACCCCGCTGCTCCTGCAGCGCCAGC
CCCAGCGCAGTGACAGCCGCCGGGCGCCCTCGACCCTCGGATAGTTGTAAAGAAGAAAGT
TCTACCCTTTCTGTCAAAATGAAGTGTGATTTTAATTGTAACCATGTTCATTCCGGACTT
AAACTGGTAAAACCTGATGACATTGGAAGACTAGTTTCCTACACCCCTGCATATTTGGAA
GGTTCCTGTAAAGACTGCATTAAAGACTATGAAAGGCTGTCATGTATTGGGTCACCGATT
GTGAGCCCTAGGATTGTAGAACTTGAAACTGAAAGCAAGCGCTTGCATAACAAGGAAAAT
CAACATGTGCAACAGACACTTAATAGTACAAATGAAATAGAAGCACTAGAGACCAGTAGA
CTTTATGAAGACAGTGGCTATTCCTCATTTTCTCTACAAAGTGGCCTCAGTGAACATGAA
GAAGGTAGCCTCCTGGAGGAGAATTTCGGTGACAGTCTACAATCCTGCCTGCTACAAATA
CAAAGCCCAGACCAATATCCCAACAAAAACTTGCTGCCAGTTCTTCATTTTGAAAAAGTG
GTTTGTTCAACATTAAAAAAGAATGCAAAACGAAATCCTAAAGTAGATCGGGAGATGCTG
AAGGAAATTATAGCCAGAGGAAATTTTAGACTGCAGAATATAATTGGCAGAAAAATGGGC
CTAGAATGTGTAGATATTCTCAGCGAACTCTTTCGAAGGGGACTCAGACATGTCTTAGCA
ACTATTTTAGCACAACTCAGTGACATGGACTTAATCAATGTGTCTAAAGTGAGCACAACT
TGGAAGAAGATCCTAGAAGATGATAAGGGGGCATTCCAGTTGTACAGTAAAGCAATACAA
AGAGTTACCGAAAACAACAATAAATTTTCACCTCATGCTTCAACCAGAGAATATGTTATG
TTCAGAACCCCACTGGCTTCTGTTCAGAAATCAGCAGCCCAGACTTCTCTCAAAAAAGAT
GCTCAAACCAAGTTATCCAATCAAGGTGATCAGAAAGGTTCTACTTATAGTCGACACAAT
GAATTCTCTGAGGTTGCCAAGACATTGAAAAAGAACGAAAGCCTCAAAGCCTGTATTCGC
TGTAATTCACCTGCAAAATATGATTGCTATTTACAACGGGCAACCTGCAAACGAGAAGGC
TGTGGATTTGATTATTGTACGAAGTGTCTCTGTAATTATCATACTACTAAAGACTGTTCA
GATGGCAAGCTCCTCAAAGCCAGTTGTAAAATAGGTCCCCTGCCTGGTACAAAGAAAAGC
AAAAAGAATTTACGAAGATTG

Protein sequence predicted based on nucleotide sequence

MSRRPCSCALRPPRCSCSASPSAVTAAGRPRPSDSCKEESSTLSVKMKCDFNCNHVHSGL
KLVKPDDIGRLVSYTPAYLEGSCKDCIKDYERLSCIGSPIVSPRIVELETESKRLHNKEN
QHVQQTLNSTNEIEALETSRLYEDSGYSSFSLQSGLSEHEEGSLLEENFGDSLQSCLLQI
QSPDQYPNKNLLPVLHFEKVVCSTLKKNAKRNPKVDREMLKEIIARGNFRLQNIIGRKMG
LECVDILSELFRRGLRHVLATILAQLSDMDLINVSKVSTTWKKILEDDKGAFQLYSKAIQ
RVTENNNKFSPHASTREYVMFRTPLASVQKSAAQTSLKKDAQTKLSNQGDQKGSTYSRHN
EFSEVAKTLKKNESLKACIRCNSPAKYDCYLQRATCKREGCGFDYCTKCLCNYHTTKDCS
DGKLLKASCKIGPLPGTKKSKKNLRRL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

FBXO5

Symbol Nomenclature :

FBXO5

SYNONYM :

EMI1; FBX5; Fbxo31

Designation :

F-box only protein 5|F-box protein Fbx5|early mitotic inhibitor 1

Full Nomenclature :

F-box protein 5

GENEID :

26271

Locus Tag :

RP1-101K10.5

GenBank Accession :

DQ895106

HGNC :

13584

MIM :

606013

Vega :

OTTHUMG00000015854

Target GenBank:

DQ895106

Reference Sequence Alignment

  

HsCD00511981      ATGAGCCGGCGCCCCTGCAGCTGCGCCCTACGGCCACCCCGCTGCTCCTGCAGCGCCAGC
NM_012177.3       ATGAGCCGGCGCCCCTGCAGCTGCGCCCTACGGCCACCCCGCTGCTCCTGCAGCGCCAGC
                  ************************************************************

HsCD00511981      CCCAGCGCAGTGACAGCCGCCGGGCGCCCTCGACCCTCGGATAGTTGTAAAGAAGAAAGT
NM_012177.3       CCCAGCGCAGTGACAGCCGCCGGGCGCCCTCGACCCTCGGATAGTTGTAAAGAAGAAAGT
                  ************************************************************

HsCD00511981      TCTACCCTTTCTGTCAAAATGAAGTGTGATTTTAATTGTAACCATGTTCATTCCGGACTT
NM_012177.3       TCTACCCTTTCTGTCAAAATGAAGTGTGATTTTAATTGTAACCATGTTCATTCCGGACTT
                  ************************************************************

HsCD00511981      AAACTGGTAAAACCTGATGACATTGGAAGACTAGTTTCCTACACCCCTGCATATTTGGAA
NM_012177.3       AAACTGGTAAAACCTGATGACATTGGAAGACTAGTTTCCTACACCCCTGCATATTTGGAA
                  ************************************************************

HsCD00511981      GGTTCCTGTAAAGACTGCATTAAAGACTATGAAAGGCTGTCATGTATTGGGTCACCGATT
NM_012177.3       GGTTCCTGTAAAGACTGCATTAAAGACTATGAAAGGCTGTCATGTATTGGGTCACCGATT
                  ************************************************************

HsCD00511981      GTGAGCCCTAGGATTGTAGAACTTGAAACTGAAAGCAAGCGCTTGCATAACAAGGAAAAT
NM_012177.3       GTGAGCCCTAGGATTGTACAACTTGAAACTGAAAGCAAGCGCTTGCATAACAAGGAAAAT
                  ****************** *****************************************

HsCD00511981      CAACATGTGCAACAGACACTTAATAGTACAAATGAAATAGAAGCACTAGAGACCAGTAGA
NM_012177.3       CAACATGTGCAACAGACACTTAATAGTACAAATGAAATAGAAGCACTAGAGACCAGTAGA
                  ************************************************************

HsCD00511981      CTTTATGAAGACAGTGGCTATTCCTCATTTTCTCTACAAAGTGGCCTCAGTGAACATGAA
NM_012177.3       CTTTATGAAGACAGTGGCTATTCCTCATTTTCTCTACAAAGTGGCCTCAGTGAACATGAA
                  ************************************************************

HsCD00511981      GAAGGTAGCCTCCTGGAGGAGAATTTCGGTGACAGTCTACAATCCTGCCTGCTACAAATA
NM_012177.3       GAAGGTAGCCTCCTGGAGGAGAATTTCGGTGACAGTCTACAATCCTGCCTGCTACAAATA
                  ************************************************************

HsCD00511981      CAAAGCCCAGACCAATATCCCAACAAAAACTTGCTGCCAGTTCTTCATTTTGAAAAAGTG
NM_012177.3       CAAAGCCCAGACCAATATCCCAACAAAAACTTGCTGCCAGTTCTTCATTTTGAAAAAGTG
                  ************************************************************

HsCD00511981      GTTTGTTCAACATTAAAAAAGAATGCAAAACGAAATCCTAAAGTAGATCGGGAGATGCTG
NM_012177.3       GTTTGTTCAACATTAAAAAAGAATGCAAAACGAAATCCTAAAGTAGATCGGGAGATGCTG
                  ************************************************************

HsCD00511981      AAGGAAATTATAGCCAGAGGAAATTTTAGACTGCAGAATATAATTGGCAGAAAAATGGGC
NM_012177.3       AAGGAAATTATAGCCAGAGGAAATTTTAGACTGCAGAATATAATTGGCAGAAAAATGGGC
                  ************************************************************

HsCD00511981      CTAGAATGTGTAGATATTCTCAGCGAACTCTTTCGAAGGGGACTCAGACATGTCTTAGCA
NM_012177.3       CTAGAATGTGTAGATATTCTCAGCGAACTCTTTCGAAGGGGACTCAGACATGTCTTAGCA
                  ************************************************************

HsCD00511981      ACTATTTTAGCACAACTCAGTGACATGGACTTAATCAATGTGTCTAAAGTGAGCACAACT
NM_012177.3       ACTATTTTAGCACAACTCAGTGACATGGACTTAATCAATGTGTCTAAAGTGAGCACAACT
                  ************************************************************

HsCD00511981      TGGAAGAAGATCCTAGAAGATGATAAGGGGGCATTCCAGTTGTACAGTAAAGCAATACAA
NM_012177.3       TGGAAGAAGATCCTAGAAGATGATAAGGGGGCATTCCAGTTGTACAGTAAAGCAATACAA
                  ************************************************************

HsCD00511981      AGAGTTACCGAAAACAACAATAAATTTTCACCTCATGCTTCAACCAGAGAATATGTTATG
NM_012177.3       AGAGTTACCGAAAACAACAATAAATTTTCACCTCATGCTTCAACCAGAGAATATGTTATG
                  ************************************************************

HsCD00511981      TTCAGAACCCCACTGGCTTCTGTTCAGAAATCAGCAGCCCAGACTTCTCTCAAAAAAGAT
NM_012177.3       TTCAGAACCCCACTGGCTTCTGTTCAGAAATCAGCAGCCCAGACTTCTCTCAAAAAAGAT
                  ************************************************************

HsCD00511981      GCTCAAACCAAGTTATCCAATCAAGGTGATCAGAAAGGTTCTACTTATAGTCGACACAAT
NM_012177.3       GCTCAAACCAAGTTATCCAATCAAGGTGATCAGAAAGGTTCTACTTATAGTCGACACAAT
                  ************************************************************

HsCD00511981      GAATTCTCTGAGGTTGCCAAGACATTGAAAAAGAACGAAAGCCTCAAAGCCTGTATTCGC
NM_012177.3       GAATTCTCTGAGGTTGCCAAGACATTGAAAAAGAACGAAAGCCTCAAAGCCTGTATTCGC
                  ************************************************************

HsCD00511981      TGTAATTCACCTGCAAAATATGATTGCTATTTACAACGGGCAACCTGCAAACGAGAAGGC
NM_012177.3       TGTAATTCACCTGCAAAATATGATTGCTATTTACAACGGGCAACCTGCAAACGAGAAGGC
                  ************************************************************

HsCD00511981      TGTGGATTTGATTATTGTACGAAGTGTCTCTGTAATTATCATACTACTAAAGACTGTTCA
NM_012177.3       TGTGGATTTGATTATTGTACGAAGTGTCTCTGTAATTATCATACTACTAAAGACTGTTCA
                  ************************************************************

HsCD00511981      GATGGCAAGCTCCTCAAAGCCAGTTGTAAAATAGGTCCCCTGCCTGGTACAAAGAAAAGC
NM_012177.3       GATGGCAAGCTCCTCAAAGCCAGTTGTAAAATAGGTCCCCTGCCTGGTACAAAGAAAAGC
                  ************************************************************

HsCD00511981      AAAAAGAATTTACGAAGATTG---
NM_012177.3       AAAAAGAATTTACGAAGATTGTGA
                  *********************

Pathways

NCI :

PLK1 signaling events

Reactome :

APC/C-mediated degradation of cell cycle proteins

Reactome :

Cell Cycle

Reactome :

Cell Cycle, Mitotic

Reactome :

E2F mediated regulation of DNA replication

Reactome :

G1/S Transition

Reactome :

G1/S-Specific Transcription

Reactome :

M Phase

Reactome :

Mitotic G1-G1/S phases

Reactome :

Mitotic M-M/G1 phases

Reactome :

Mitotic Metaphase and Anaphase

Reactome :

Mitotic Metaphase/Anaphase Transition

Reactome :

Phosphorylation of Emi1

Reactome :

Regulation of APC/C activators between G1/S and early anaphase

Reactome :

Regulation of mitotic cell cycle

Reactome :

SCF-beta-TrCP mediated degradation of Emi1

Protein

HPRD :

09348

Clone

Cloning Information :

604910

Original Clone ID :

ccsbBroadEn_08010

Organism

Species Specific ID:

26271

Genome

Chromosome :

Map Location :

6q25.2

Ensembl :

ENSG00000112029

Reagents

Labome :

FBXO5-antibody