DNASU Plasmid | CRTAC1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: CRTAC1

Gene Name: cartilage acidic protein 1

Original Clone ID:

ccsbBroadEn_12161

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC034245.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512021

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1354nts

Open reading frame: 1 to 1354

Reference Sequence Alignment

ATGGACCCTGAGGCCAGTGACCTCTCCCGGGGCATTCTGGCGCTCAGAGATGTGGCTGCT
GAGGCTGGGGTCAGCAAATATACAGGGGGCCGAGGCGTCAGCGTGGGCCCCATCCTCAGC
AGCAGTGCCTCGGATATCTTCTGCGACAATGAGAATGGGCCTAACTTCCTTTTCCACAAC
CGGGGCGATGGCACCTTTGTGGACGCTGCGGCCAGTGCTGGTGTGGACGACCCCCACCAG
CATGGGCGAGGTGTCGCCCTGGCTGACTTCAACCGTGATGGCAAAGTGGACATCGTCTAT
GGCAACTGGAATGGCCCCCACCGCCTCTATCTGCAAATGAGCACCCATGGGAAGGTCCGC
TTCCGGGACATCGCCTCACCCAAGTTCTCCATGCCCTCCCCTGTCCGCACGGTCATCACC
GCCGACTTTGACAATGACCAGGAGCTGGAGATCTTCTTCAACAACATTGCCTACCGCAGC
TCCTCAGCCAACCGCCTCTTCCGCGTCATCCGTAGAGAGCACGGAGACCCCCTCATCGAG
GAGCTCAATCCCGGCGACGCCTTGGAGCCTGAGGGCCGGGGCACAGGGGGTGTGGTGACC
GACTTCGACGGAGACGGGATGCTGGACCTCATCTTGTCCCATGGAGAGTCCATGGCTCAG
CCGCTGTCCGTCTTCCGGGGCAATCAGGGCTTCAACAACAACTGGCTGCGAGTGGTGCCA
CGCACCCGGTTTGGGGCCTTTGCCAGGGGAGCTAAGGTCGTGCTCTACACCAAGAAGAGT
GGGGCCCACCTGAGGATCATCGACGGGGGCTCAGGCTACCTGTGTGAGATGGAGCCCGTG
GCACACTTTGGCCTGGGGAAGGATGAAGCCAGCAGTGTGGAGGTGACGTGGCCAGATGGC
AAGATGGTGAGCCGGAACGTGGCCAGCGGGGAGATGAACTCAGTGCTGGAGATCCTCTAC
CCCCGGGATGAGGACACACTTCAGGACCCAGCCCCACTGGAGTGTGGCCAAGGATTCTCC
CAGCAGGAAAATGGCCATTGCATGGACACCAATGAATGCATCCAGTTCCCATTCGTGTGC
CCTCGAGACAAGCCCGTATGTGTCAACACCTATGGAAGCTACAGGTGCCGGACCAACAAG
AAGTGCAGTCGGGGCTACGAGCCCAACGAGGATGGCACAGCCTGCGTGGGGACTCTCGGC
CAGTCACCGGGCCCCCGCCCCACCACCCCCACCGCTGCTGCTGCCACTGCCGCTGCTGCT
GCCGCTGCTGGAGCTGCCACTGCTGCACCGGTCCTCGTAGATGGAGATCTCAATCTGGGG
TCGGTGGTTAAGGAGAGCTGCGAGCCCAGCTGC

Protein sequence predicted based on nucleotide sequence

MDPEASDLSRGILALRDVAAEAGVSKYTGGRGVSVGPILSSSASDIFCDNENGPNFLFHN
RGDGTFVDAAASAGVDDPHQHGRGVALADFNRDGKVDIVYGNWNGPHRLYLQMSTHGKVR
FRDIASPKFSMPSPVRTVITADFDNDQELEIFFNNIAYRSSSANRLFRVIRREHGDPLIE
ELNPGDALEPEGRGTGGVVTDFDGDGMLDLILSHGESMAQPLSVFRGNQGFNNNWLRVVP
RTRFGAFARGAKVVLYTKKSGAHLRIIDGGSGYLCEMEPVAHFGLGKDEASSVEVTWPDG
KMVSRNVASGEMNSVLEILYPRDEDTLQDPAPLECGQGFSQQENGHCMDTNECIQFPFVC
PRDKPVCVNTYGSYRCRTNKKCSRGYEPNEDGTACVGTLGQSPGPRPTTPTAAAATAAAA
AAAGAATAAPVLVDGDLNLGSVVKESCEPSC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

CRTAC1

Symbol Nomenclature :

CRTAC1

SYNONYM :

ASPIC; ASPIC1; CEP-68

Designation :

acidic secreted protein in cartilage|chondrocyte expressed protein 68 kDa CEP-68

Full Nomenclature :

cartilage acidic protein 1

GENEID :

55118

Locus Tag :

RP11-459F3.1

HGNC :

14882

MIM :

606276

Vega :

OTTHUMG00000018871

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00512021      ------------------------------------------------------------
NM_018058.6       ATGGCTCCGAGCGCTGACCCCGGCATGTCCAGGATGTTACCGTTCCTGCTGCTGCTCTGG
                                                                              

HsCD00512021      ------------------------------------------------------------
NM_018058.6       TTTCTGCCCATCACTGAGGGGTCCCAGCGGGCTGAACCCATGTTCACTGCAGTCACCAAC
                                                                              

HsCD00512021      ------------------------------------------------------------
NM_018058.6       TCAGTTCTGCCTCCTGACTATGACAGTAATCCCACCCAGCTCAACTATGGTGTGGCAGTT
                                                                              

HsCD00512021      ------------------------------------------------------------
NM_018058.6       ACTGATGTGGACCATGATGGGGACTTTGAGATCGTCGTGGCGGGGTACAATGGACCCAAC
                                                                              

HsCD00512021      ------------------------------------------------------------
NM_018058.6       CTGGTTCTGAAGTATGACCGGGCCCAGAAGCGGCTGGTGAACATCGCGGTCGATGAGCGC
                                                                              

HsCD00512021      ------------------------------------------------------------
NM_018058.6       AGCTCACCCTACTACGCGCTGCGGGACCGGCAGGGGAACGCCATCGGGGTCACAGCCTGC
                                                                              

HsCD00512021      ------------------------------------------------------------
NM_018058.6       GACATCGACGGGGACGGCCGGGAGGAGATCTACTTCCTCAACACCAATAATGCCTTCTCG
                                                                              

HsCD00512021      ------------------------------------------------------------
NM_018058.6       GGGGTGGCCACGTACACCGACAAGTTGTTCAAGTTCCGCAATAACCGGTGGGAAGACATC
                                                                              

HsCD00512021      ------------------------------------------------------------
NM_018058.6       CTGAGCGATGAGGTCAACGTGGCCCGTGGTGTGGCCAGCCTCTTTGCCGGACGCTCTGTG
                                                                              

HsCD00512021      ------------------------------------------------------------
NM_018058.6       GCCTGTGTGGACAGAAAGGGCTCTGGACGCTACTCTATCTACATTGCCAATTACGCCTAC
                                                                              

HsCD00512021      ------------------------------ATGGACCCTGAGGCCAGTGACCTCTCCCGG
NM_018058.6       GGTAATGTGGGCCCTGATGCCCTCATTGAAATGGACCCTGAGGCCAGTGACCTCTCCCGG
                                                ******************************

HsCD00512021      GGCATTCTGGCGCTCAGAGATGTGGCTGCTGAGGCTGGGGTCAGCAAATATACAGGGGGC
NM_018058.6       GGCATTCTGGCGCTCAGAGATGTGGCTGCTGAGGCTGGGGTCAGCAAATATACAGGGGGC
                  ************************************************************

HsCD00512021      CGAGGCGTCAGCGTGGGCCCCATCCTCAGCAGCAGTGCCTCGGATATCTTCTGCGACAAT
NM_018058.6       CGAGGCGTCAGCGTGGGCCCCATCCTCAGCAGCAGTGCCTCGGATATCTTCTGCGACAAT
                  ************************************************************

HsCD00512021      GAGAATGGGCCTAACTTCCTTTTCCACAACCGGGGCGATGGCACCTTTGTGGACGCTGCG
NM_018058.6       GAGAATGGGCCTAACTTCCTTTTCCACAACCGGGGCGATGGCACCTTTGTGGACGCTGCG
                  ************************************************************

HsCD00512021      GCCAGTGCTGGTGTGGACGACCCCCACCAGCATGGGCGAGGTGTCGCCCTGGCTGACTTC
NM_018058.6       GCCAGTGCTGGTGTGGACGACCCCCACCAGCATGGGCGAGGTGTCGCCCTGGCTGACTTC
                  ************************************************************

HsCD00512021      AACCGTGATGGCAAAGTGGACATCGTCTATGGCAACTGGAATGGCCCCCACCGCCTCTAT
NM_018058.6       AACCGTGATGGCAAAGTGGACATCGTCTATGGCAACTGGAATGGCCCCCACCGCCTCTAT
                  ************************************************************

HsCD00512021      CTGCAAATGAGCACCCATGGGAAGGTCCGCTTCCGGGACATCGCCTCACCCAAGTTCTCC
NM_018058.6       CTGCAAATGAGCACCCATGGGAAGGTCCGCTTCCGGGACATCGCCTCACCCAAGTTCTCC
                  ************************************************************

HsCD00512021      ATGCCCTCCCCTGTCCGCACGGTCATCACCGCCGACTTTGACAATGACCAGGAGCTGGAG
NM_018058.6       ATGCCCTCCCCTGTCCGCACGGTCATCACCGCCGACTTTGACAATGACCAGGAGCTGGAG
                  ************************************************************

HsCD00512021      ATCTTCTTCAACAACATTGCCTACCGCAGCTCCTCAGCCAACCGCCTCTTCCGCGTCATC
NM_018058.6       ATCTTCTTCAACAACATTGCCTACCGCAGCTCCTCAGCCAACCGCCTCTTCCGCGTCATC
                  ************************************************************

HsCD00512021      CGTAGAGAGCACGGAGACCCCCTCATCGAGGAGCTCAATCCCGGCGACGCCTTGGAGCCT
NM_018058.6       CGTAGAGAGCACGGAGACCCCCTCATCGAGGAGCTCAATCCCGGCGACGCCTTGGAGCCT
                  ************************************************************

HsCD00512021      GAGGGCCGGGGCACAGGGGGTGTGGTGACCGACTTCGACGGAGACGGGATGCTGGACCTC
NM_018058.6       GAGGGCCGGGGCACAGGGGGTGTGGTGACCGACTTCGACGGAGACGGGATGCTGGACCTC
                  ************************************************************

HsCD00512021      ATCTTGTCCCATGGAGAGTCCATGGCTCAGCCGCTGTCCGTCTTCCGGGGCAATCAGGGC
NM_018058.6       ATCTTGTCCCATGGAGAGTCCATGGCTCAGCCGCTGTCCGTCTTCCGGGGCAATCAGGGC
                  ************************************************************

HsCD00512021      TTCAACAACAACTGGCTGCGAGTGGTGCCACGCACCCGGTTTGGGGCCTTTGCCAGGGGA
NM_018058.6       TTCAACAACAACTGGCTGCGAGTGGTGCCACGCACCCGGTTTGGGGCCTTTGCCAGGGGA
                  ************************************************************

HsCD00512021      GCTAAGGTCGTGCTCTACACCAAGAAGAGTGGGGCCCACCTGAGGATCATCGACGGGGGC
NM_018058.6       GCTAAGGTCGTGCTCTACACCAAGAAGAGTGGGGCCCACCTGAGGATCATCGACGGGGGC
                  ************************************************************

HsCD00512021      TCAGGCTACCTGTGTGAGATGGAGCCCGTGGCACACTTTGGCCTGGGGAAGGATGAAGCC
NM_018058.6       TCAGGCTACCTGTGTGAGATGGAGCCCGTGGCACACTTTGGCCTGGGGAAGGATGAAGCC
                  ************************************************************

HsCD00512021      AGCAGTGTGGAGGTGACGTGGCCAGATGGCAAGATGGTGAGCCGGAACGTGGCCAGCGGG
NM_018058.6       AGCAGTGTGGAGGTGACGTGGCCAGATGGCAAGATGGTGAGCCGGAACGTGGCCAGCGGG
                  ************************************************************

HsCD00512021      GAGATGAACTCAGTGCTGGAGATCCTCTACCCCCGGGATGAGGACACACTTCAGGACCCA
NM_018058.6       GAGATGAACTCAGTGCTGGAGATCCTCTACCCCCGGGATGAGGACACACTTCAGGACCCA
                  ************************************************************

HsCD00512021      GCCCCACTGGAGTGTGGCCAAGGATTCTCCCAGCAGGAAAATGGCCATTGCATGGACACC
NM_018058.6       GCCCCACTGGAGTGTGGCCAAGGATTCTCCCAGCAGGAAAATGGCCATTGCATGGACACC
                  ************************************************************

HsCD00512021      AATGAATGCATCCAGTTCCCATTCGTGTGCCCTCGAGACAAGCCCGTATGTGTCAACACC
NM_018058.6       AATGAATGCATCCAGTTCCCATTCGTGTGCCCTCGAGACAAGCCCGTATGTGTCAACACC
                  ************************************************************

HsCD00512021      TATGGAAGCTACAGGTGCCGGACCAACAAGAAGTGCAGTCGGGGCTACGAGCCCAACGAG
NM_018058.6       TATGGAAGCTACAGGTGCCGGACCAACAAGAAGTGCAGTCGGGGCTACGAGCCCAACGAG
                  ************************************************************

HsCD00512021      GATGGCACAGCCTGCGTGGGGACTCTCGGCCAGTCACCGGGCCCCCGCCCCACCACCCCC
NM_018058.6       GATGGCACAGCCTGCGTGGGGACTCTCGGCCAGTCACCGGGCCCCCGCCCCACCACCCCC
                  ************************************************************

HsCD00512021      ACCGCTGCTGCTGCCACTGCCGCTGCTGCTGCCGCTGCTGGAGCTGCCACTGCTGCACCG
NM_018058.6       ACCGCTGCTGCTGCCACTGCCGCTGCTGCTGCCGCTGCTGGAGCTGCCACTGCTGCACCG
                  ************************************************************

HsCD00512021      GTCCTCGTAGATGGAGATCTCAATCTGGGGTCGGTGGTTAAGGAGAGCTGCGAGCCCAGC
NM_018058.6       GTCCTCGTAGATGGAGATCTCAATCTGGGGTCGGTGGTTAAGGAGAGCTGCGAGCCCAGC
                  ************************************************************

HsCD00512021      TGC---
NM_018058.6       TGCTGA
                  ***