DNASU Plasmid | TUBG1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TUBG2

Gene Name: tubulin, gamma 2

Original Clone ID:

ccsbBroadEn_01725

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC000619.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512063

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1354nts

Open reading frame: 1 to 1354

Reference Sequence Alignment

ATGCCGAGGGAAATCATCACCCTACAGTTGGGCCAGTGCGGCAATCAGATTGGGTTCGAG
TTCTGGAAACAGCTGTGCGCCGAGCATGGTATCAGCCCCGAGGGCATCGTGGAGGAGTTC
GCCACCGAGGGCACTGACCGCAAGGACGTCTTTTTCTACCAGGCAGACGATGAGCACTAC
ATCCCCCGGGCCGTGCTGCTGGACTTGGAACCCCGGGTGATCCACTCCATCCTCAACTCC
CCCTATGCCAAGCTCTACAACCCAGAGAACATCTACCTGTCGGAACATGGAGGAGGAGCT
GGCAACAACTGGGCCAGCGGATTCTCCCAGGGAGAAAAGATCCATGAGGACATTTTTGAC
ATCATAGACCGGGAGGCAGATGGTAGTGACAGTCTAGAGGGCTTTGTGCTGTGTCACTCC
ATTGCTGGGGGGACAGGCTCTGGACTGGGTTCCTACCTCTTAGAACGGCTGAATGACAGG
TATCCTAAGAAGCTGGTGCAGACATACTCAGTGTTTCCCAACCAGGACGAGATGAGCGAT
GTGGTGGTCCAGCCTTACAATTCACTCCTCACACTCAAGAGGCTGACGCAGAATGCAGAC
TGTGTGGTGGTGCTGGACAACACAGCCCTGAACCGGATTGCCACAGACCGCCTGCACATC
CAGAACCCATCCTTCTCCCAGATCAACCAGCTGGTGTCTACCATCATGTCAGCCAGCACC
ACCACCCTGCGCTACCCTGGCTACATGAACAATGACCTCATCGGCCTCATCGCCTCGCTC
ATTCCCACCCCACGGCTCCACTTCCTCATGACCGGCTACACCCCTCTCACTACGGACCAG
TCAGTGGCCAGCGTGAGGAAGACCACGGTCCTGGATGTCATGAGGCGGCTGCTGCAGCCC
AAGAACGTGATGGTGTCCACAGGCCGAGACCGCCAGACCAACCACTGCTACATCGCCATC
CTCAACATCATCCAGGGAGAGGTGGACCCCACCCAGGTCCACAAGAGCTTGCAGAGGATC
CGGGAACGCAAGTTGGCCAACTTCATCCCGTGGGGCCCCGCCAGCATCCAGGTGGCCCTG
TCGAGGAAGTCTCCCTACCTGCCCTCGGCCCACCGGGTCAGCGGGCTCATGATGGCCAAC
CACACCAGCATCTCCTCGCTCTTCGAGAGAACCTGTCGCCAGTATGACAAGCTGCGTAAG
CGGGAGGCCTTCCTGGAGCAGTTCCGCAAGGAGGACATGTTCAAGGACAACTTTGATGAG
ATGGACACATCCAGGGAGATTGTGCAGCAGCTCATCGATGAGTACCATGCGGCCACACGG
CCAGACTACATCTCCTGGGGCACCCAGGAGCAG

Protein sequence predicted based on nucleotide sequence

MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHY
IPRAVLLDLEPRVIHSILNSPYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFD
IIDREADGSDSLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSD
VVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSAST
TTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQP
KNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVAL
SRKSPYLPSAHRVSGLMMANHTSISSLFERTCRQYDKLRKREAFLEQFRKEDMFKDNFDE
MDTSREIVQQLIDEYHAATRPDYISWGTQEQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

TUBG2

Symbol Nomenclature :

TUBG2

Designation :

gamma-2-tubulin|tubulin gamma-2 chain

Full Nomenclature :

tubulin, gamma 2

GENEID :

27175

GenBank Accession :

DQ895110

HGNC :

12419

MIM :

605785

Vega :

OTTHUMG00000180640

Target GenBank:

DQ895110

Reference Sequence Alignment

  

HsCD00512063        ATGCCGAGGGAAATCATCACCCTACAGTTGGGCCAGTGCGGCAATCAGATTGGGTTCGAG
XM_005257222.1      ATGCCCCGGGAGATCATCACCCTGCAGCTGGGCCAGTGCGGCAACCAGATTGGGTTCGAG
NM_016437.2         ATGCCCCGGGAGATCATCACCCTGCAGCTGGGCCAGTGCGGCAACCAGATTGGGTTCGAG
NM_001070.4         ATGCCGAGGGAAATCATCACCCTACAGTTGGGCCAGTGCGGCAATCAGATTGGGTTCGAG
                    ***** .****.***********.*** **************** ***************

HsCD00512063        TTCTGGAAACAGCTGTGCGCCGAGCATGGTATCAGCCCCGAGGGCATCGTGGAGGAGTTC
XM_005257222.1      TTCTGGAAACAGCTGTGCGCCGAGCATGGTATCAGCCCCGAGGGCATCGTGGAGGAATTC
NM_016437.2         TTCTGGAAACAGCTGTGCGCCGAGCATGGTATCAGCCCCGAGGGCATCGTGGAGGAATTC
NM_001070.4         TTCTGGAAACAGCTGTGCGCCGAGCATGGTATCAGCCCCGAGGGCATCGTGGAGGAGTTC
                    ********************************************************.***

HsCD00512063        GCCACCGAGGGCACTGACCGCAAGGACGTCTTTTTCTACCAGGCAGACGATGAGCACTAC
XM_005257222.1      GCCACCGAGGGCACTGACCGCAAGGACGTCTTTTTCTACCAGGCAGACGATGAGCACTAC
NM_016437.2         GCCACCGAGGGCACTGACCGCAAGGACGTCTTTTTCTACCAGGCAGACGATGAGCACTAC
NM_001070.4         GCCACCGAGGGCACTGACCGCAAGGACGTCTTTTTCTACCAGGCAGACGATGAGCACTAC
                    ************************************************************

HsCD00512063        ATCCCCCGGGCCGTGCTGCTGGACTTGGAACCCCGGGTGATCCACTCCATCCTCAACTCC
XM_005257222.1      ATCCCCCGGGCCGTGCTGCTGGACTTGGAACCCCGGGTGATCCACTCCATCCTCAACTCC
NM_016437.2         ATCCCCCGGGCCGTGCTGCTGGACTTGGAACCCCGGGTGATCCACTCCATCCTCAACTCC
NM_001070.4         ATCCCCCGGGCCGTGCTGCTGGACTTGGAACCCCGGGTGATCCACTCCATCCTCAACTCC
                    ************************************************************

HsCD00512063        CCCTATGCCAAGCTCTACAACCCAGAGAACATCTACCTGTCGGAACATGGAGGAGGAGCT
XM_005257222.1      CCCTATGCCAAGCTCTACAACCCAGAGAACATCTACCTGTCGGAACATGGAGGAGGAGCT
NM_016437.2         CCCTATGCCAAGCTCTACAACCCAGAGAACATCTACCTGTCGGAACATGGAGGAGGAGCT
NM_001070.4         CCCTATGCCAAGCTCTACAACCCAGAGAACATCTACCTGTCGGAACATGGAGGAGGAGCT
                    ************************************************************

HsCD00512063        GGCAACAACTGGGCCAGCGGATTCTCCCAGGGAGAAAAGATCCATGAGGACATTTTTGAC
XM_005257222.1      GGCAACAACTGGGCCAGCGGATTCTCCCAGGGTGAGAAAATTCATGAAGACATCTTTGAC
NM_016437.2         GGCAACAACTGGGCCAGCGGATTCTCCCAGGGTGAGAAAATTCATGAAGACATCTTTGAC
NM_001070.4         GGCAACAACTGGGCCAGCGGATTCTCCCAGGGAGAAAAGATCCATGAGGACATTTTTGAC
                    ********************************:**.**.** *****.***** ******

HsCD00512063        ATCATAGACCGGGAGGCAGATGGTAGTGACAGTCTAG-----------------------
XM_005257222.1      ATCATAGACCGAGAAGCAGATGGAAGTGACAGTTTGGAGCTCCAGAACAGCTCCAGAGTG
NM_016437.2         ATCATAGACCGAGAAGCAGATGGAAGTGACAGTTTG------------------------
NM_001070.4         ATCATAGACCGGGAGGCAGATGGTAGTGACAGTCTAG-----------------------
                    ***********.**.********:********* *.                        

HsCD00512063        ----AGGGCTTTGTGCTGTGTCACTCCATTGCTGGGGGGACAGGCTCTGGACTGGGTTCC
XM_005257222.1      ATAAAGGGCTTCGTGCTGTGTCACTCCATCGCTGGGGGTACGGGTTCTGGCCTGGGCTCC
NM_016437.2         ---GAGGGCTTCGTGCTGTGTCACTCCATCGCTGGGGGTACGGGTTCTGGCCTGGGCTCC
NM_001070.4         ----AGGGCTTTGTGCTGTGTCACTCCATTGCTGGGGGGACAGGCTCTGGACTGGGTTCC
                        ******* ***************** ******** **.** *****.***** ***

HsCD00512063        TACCTCTTAGAACGGCTGAATGACAGGTATCCTAAGAAGCTGGTGCAGACATACTCAGTG
XM_005257222.1      TACCTCCTGGAGCGACTGAATGACAGGTACCCCAAGAAGCTAGTGCAGACTTATTCAGTG
NM_016437.2         TACCTCCTGGAGCGACTGAATGACAGGTACCCCAAGAAGCTAGTGCAGACTTATTCAGTG
NM_001070.4         TACCTCTTAGAACGGCTGAATGACAGGTATCCTAAGAAGCTGGTGCAGACATACTCAGTG
                    ****** *.**.**.************** ** ********.********:** ******

HsCD00512063        TTTCCCAACCAGGACGAGATGAGCGATGTGGTGGTCCAGCCTTACAATTCACTCCTCACA
XM_005257222.1      TTTCCCTACCAGGACGAGATGAGCGACGTAGTGGTTCAGCCCTACAATTCACTCCTGACA
NM_016437.2         TTTCCCTACCAGGACGAGATGAGCGACGTAGTGGTTCAGCCCTACAATTCACTCCTGACA
NM_001070.4         TTTCCCAACCAGGACGAGATGAGCGATGTGGTGGTCCAGCCTTACAATTCACTCCTCACA
                    ******:******************* **.***** ***** ************** ***

HsCD00512063        CTCAAGAGGCTGACGCAGAATGCAGACTGTGTGGTGGTGCTGGACAACACAGCCCTGAAC
XM_005257222.1      CTCAAGAGGCTGACGCAGAACGCAGATTGTGTGGTGGTGCTGGACAACACAGCCCTGAAC
NM_016437.2         CTCAAGAGGCTGACGCAGAACGCAGATTGTGTGGTGGTGCTGGACAACACAGCCCTGAAC
NM_001070.4         CTCAAGAGGCTGACGCAGAATGCAGACTGTGTGGTGGTGCTGGACAACACAGCCCTGAAC
                    ******************** ***** *********************************

HsCD00512063        CGGATTGCCACAGACCGCCTGCACATCCAGAACCCATCCTTCTCCCAGATCAACCAGCTG
XM_005257222.1      CGGATTGCCACAGACCGCCTGCACATCCAGAACCCGTCCTTCTCCCAGATCAACCAGCTG
NM_016437.2         CGGATTGCCACAGACCGCCTGCACATCCAGAACCCGTCCTTCTCCCAGATCAACCAGCTG
NM_001070.4         CGGATTGCCACAGACCGCCTGCACATCCAGAACCCATCCTTCTCCCAGATCAACCAGCTG
                    ***********************************.************************

HsCD00512063        GTGTCTACCATCATGTCAGCCAGCACCACCACCCTGCGCTACCCTGGCTACATGAACAAT
XM_005257222.1      GTGTCCACCATCATGTCGGCCAGCACCACCACCCTGCGCTACCCCGGCTACATGAACAAT
NM_016437.2         GTGTCCACCATCATGTCGGCCAGCACCACCACCCTGCGCTACCCCGGCTACATGAACAAT
NM_001070.4         GTGTCTACCATCATGTCAGCCAGCACCACCACCCTGCGCTACCCTGGCTACATGAACAAT
                    ***** ***********.************************** ***************

HsCD00512063        GACCTCATCGGCCTCATCGCCTCGCTCATTCCCACCCCACGGCTCCACTTCCTCATGACC
XM_005257222.1      GACCTCATCGGCCTCATCGCCTCGCTCATTCCCACCCCACGGCTCCACTTCCTCATGACC
NM_016437.2         GACCTCATCGGCCTCATCGCCTCGCTCATTCCCACCCCACGGCTCCACTTCCTCATGACC
NM_001070.4         GACCTCATCGGCCTCATCGCCTCGCTCATTCCCACCCCACGGCTCCACTTCCTCATGACC
                    ************************************************************

HsCD00512063        GGCTACACCCCTCTCACTACGGACCAGTCAGTGGCCAGCGTGAGGAAGACCACGGTCCTG
XM_005257222.1      GGCTACACCCCGCTCACTACAGACCAGTCAGTGGCCAGCGTGAGGAAGACCACGGTCCTG
NM_016437.2         GGCTACACCCCGCTCACTACAGACCAGTCAGTGGCCAGCGTGAGGAAGACCACGGTCCTG
NM_001070.4         GGCTACACCCCTCTCACTACGGACCAGTCAGTGGCCAGCGTGAGGAAGACCACGGTCCTG
                    *********** ********.***************************************

HsCD00512063        GATGTCATGAGGCGGCTGCTGCAGCCCAAGAACGTGATGGTGTCCACAGGCCGAGACCGC
XM_005257222.1      GATGTCATGAGGCGGCTGCTGCAGCCCAAGAACGTGATGGTGTCCACAGGCCGAGACCGC
NM_016437.2         GATGTCATGAGGCGGCTGCTGCAGCCCAAGAACGTGATGGTGTCCACAGGCCGAGACCGC
NM_001070.4         GATGTCATGAGGCGGCTGCTGCAGCCCAAGAACGTGATGGTGTCCACAGGCCGAGACCGC
                    ************************************************************

HsCD00512063        CAGACCAACCACTGCTACATCGCCATCCTCAACATCATCCAGGGAGAGGTGGACCCCACC
XM_005257222.1      CAGACCAACCACTGCTACATCGCCATCCTCAACATCATCCAGGGAGAGGTGGACCCCACC
NM_016437.2         CAGACCAACCACTGCTACATCGCCATCCTCAACATCATCCAGGGAGAGGTGGACCCCACC
NM_001070.4         CAGACCAACCACTGCTACATCGCCATCCTCAACATCATCCAGGGAGAGGTGGACCCCACC
                    ************************************************************

HsCD00512063        CAGGTCCACAAGAGCTTGCAGAGGATCCGGGAACGCAAGTTGGCCAACTTCATCCCGTGG
XM_005257222.1      CAGGTCCACAAGAGCCTGCAGAGGATCCGGGAACGGAAGTTGGCCAACTTCATCCCGTGG
NM_016437.2         CAGGTCCACAAGAGCCTGCAGAGGATCCGGGAACGGAAGTTGGCCAACTTCATCCCGTGG
NM_001070.4         CAGGTCCACAAGAGCTTGCAGAGGATCCGGGAACGCAAGTTGGCCAACTTCATCCCGTGG
                    *************** ******************* ************************

HsCD00512063        GGCCCCGCCAGCATCCAGGTGGCCCTGTCGAGGAAGTCTCCCTACCTGCCCTCGGCCCAC
XM_005257222.1      GGCCCCGCCAGCATCCAGGTGGCCCTGTCGAGGAAGTCTCCCTACCTGCCCTCGGCCCAC
NM_016437.2         GGCCCCGCCAGCATCCAGGTGGCCCTGTCGAGGAAGTCTCCCTACCTGCCCTCGGCCCAC
NM_001070.4         GGCCCCGCCAGCATCCAGGTGGCCCTGTCGAGGAAGTCTCCCTACCTGCCCTCGGCCCAC
                    ************************************************************

HsCD00512063        CGGGTCAGCGGGCTCATGATGGCCAACCACACCAGCATCTCCTCGCTCTTCGAGAGAACC
XM_005257222.1      CGGGTCAGCGGGCTCATGATGGCCAACCACACCAGCATCTCCTCGCTCTTTGAAAGTTCC
NM_016437.2         CGGGTCAGCGGGCTCATGATGGCCAACCACACCAGCATCTCCTCGCTCTTTGAAAGTTCC
NM_001070.4         CGGGTCAGCGGGCTCATGATGGCCAACCACACCAGCATCTCCTCGCTCTTCGAGAGAACC
                    ************************************************** **.**::**

HsCD00512063        TGTCGCCAGTATGACAAGCTGCGTAAGCGGGAGGCCTTCCTGGAGCAGTTCCGCAAGGAG
XM_005257222.1      TGCCAGCAGTTTGACAAGCTGCGGAAGCGGGATGCCTTCCTCGAGCAGTTCCGTAAGGAG
NM_016437.2         TGCCAGCAGTTTGACAAGCTGCGGAAGCGGGATGCCTTCCTCGAGCAGTTCCGTAAGGAG
NM_001070.4         TGTCGCCAGTATGACAAGCTGCGTAAGCGGGAGGCCTTCCTGGAGCAGTTCCGCAAGGAG
                    ** *. ****:************ ******** ******** *********** ******

HsCD00512063        GACATGTTCAAGGACAACTTTGATGAGATGGACACATCCAGGGAGATTGTGCAGCAGCTC
XM_005257222.1      GACATGTTCAAGGACAACTTTGATGAGATGGACAGGTCTAGGGAGGTTGTTCAGGAGCTC
NM_016437.2         GACATGTTCAAGGACAACTTTGATGAGATGGACAGGTCTAGGGAGGTTGTTCAGGAGCTC
NM_001070.4         GACATGTTCAAGGACAACTTTGATGAGATGGACACATCCAGGGAGATTGTGCAGCAGCTC
                    ********************************** .** ******.**** *** *****

HsCD00512063        ATCGATGAGTACCATGCGGCCACACGGCCAGACTACATCTCCTGGGGCACCCAGGAGCAG
XM_005257222.1      ATTGATGAGTACCATGCGGCCACCCAGCCAGACTACATTTCCTGGGGCACCCAGGAGCAG
NM_016437.2         ATTGATGAGTACCATGCGGCCACCCAGCCAGACTACATTTCCTGGGGCACCCAGGAGCAG
NM_001070.4         ATCGATGAGTACCATGCGGCCACACGGCCAGACTACATCTCCTGGGGCACCCAGGAGCAG
                    ** ********************.*.************ *********************

HsCD00512063        ---
XM_005257222.1      TGA
NM_016437.2         TGA
NM_001070.4         TGA

Pathways

NCI :

PLK1 signaling events

Reactome :

Cell Cycle

Reactome :

Cell Cycle, Mitotic

Reactome :

Centrosome maturation

Reactome :

G2/M Transition

Reactome :

Loss of Nlp from mitotic centrosomes

Reactome :

Loss of proteins required for interphase microtubule organizationÿ from the centrosome

Reactome :

Mitotic G2-G2/M phases

Reactome :

Recruitment of mitotic centrosome proteins and complexes

Reactome :

Recruitment of NuMA to mitotic centrosomes

Reactome :

Regulation of PLK1 Activity at G2/M Transition

Protein

SMART domain :

Tubulin : Tubulin/FtsZ family, GTPase domain

SMART domain :

Tubulin_C : Tubulin/FtsZ family, C-terminal domain

HPRD :

05778

Clone

Cloning Information :

604992

Original Clone ID :

ccsbBroadEn_01725

Organism

Species Specific ID:

7283

Genome

Chromosome :

Map Location :

17q21

Ensembl :

ENSG00000037042

Reagents

Labome :

gamma-tubulin-antibody