DNASU Plasmid | ALDH3A1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ALDH3A1

Gene Name: aldehyde dehydrogenase 3 family, member A1

Original Clone ID:

ccsbBroadEn_05801

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC004370.1,BC008892.2,BC021194.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512103

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1359nts

Open reading frame: 1 to 1359

Reference Sequence Alignment

ATGAGCAAGATCAGCGAGGCCGTGAAGCGCGCCCGCGCCGCCTTCAGCTCGGGCAGGACC
CGTCCGCTGCAGTTCCGGATCCAGCAGCTGGAGGCGCTGCAGCGCCTGATCCAGGAGCAG
GAGCAGGAGCTGGTGGGCGCGCTGGCCGCAGACCTGCACAAGAATGAATGGAACGCCTAC
TATGAGGAGGTGGTGTACGTCCTAGAGGAGATCGAGTACATGATCCAGAAGCTCCCTGAG
TGGGCCGCGGATGAGCCCGTGGAGAAGACGCCCCAGACTCAGCAGGACGAGCTCTACATC
CACTCGGAGCCACTGGGCGTGGTCCTCGTCATTGGCACCTGGAACTACCCCTTCAACCTC
ACCATCCAGCCCATGGTGGGCGCCATCGCTGCAGGGAACGCAGTGGTCCTCAAGCCCTCG
GAGCTGAGTGAGAACATGGCGAGCCTGCTGGCTACCATCATCCCCCAGTACCTGGACAAG
GATCTGTACCCAGTAATCAATGGGGGTGTCCCTGAGACCACGGAGCTGCTCAAGGAGAGG
TTCGACCATATCCTGTACACGGGCAGCACGGGGGTGGGGAAGATCATCATGACGGCTGCT
GCCAAGCACCTGACCCCTGTCACGCTGGAGCTGGGAGGGAAGAGTCCCTGCTACGTGGAC
AAGAACTGTGACCTGGACGTGGCCTGCCGACGCATCGCCTGGGGGAAATTCATGAACAGT
GGCCAGACCTGCGTGGCCCCAGACTACATCCTCTGTGACCCCTCGATCCAGAACCAAATT
GTGGAGAAGCTCAAGAAGTCACTGAAAGAGTTCTACGGGGAAGATGCTAAGAAATCCCGG
GACTATGGAAGAATCATTAGTGCCCGGCACTTCCAGAGGGTGATGGGCCTGATTGAGGGC
CAGAAGGTGGCTTATGGGGGCACCGGGGATGCCGCCACTCGCTACATAGCCCCCACCATC
CTCACGGACGTGGACCCCCAGTCCCCGGTGATGCAAGAGGAGATCTTCGGGCCTGTGCTG
CCCATCGTGTGCGTGCGCAGCCTGGAGGAGGCCATCCAGTTCATCAACCAGCGTGAGAAG
CCCCTGGCCCTCTACATGTTCTCCAGCAACGACAAGGTGATTAAGAAGATGATTGCAGAG
ACATCCAGTGGTGGGGTGGCGGCCAACGATGTCATCGTCCACATCACCTTGCACTCTCTG
CCCTTCGGGGGCGTGGGGAACAGCGGCATGGGATCCTACCATGGCAAGAAGAGCTTCGAG
ACTTTCTCTCACCGCCGCTCTTGCCTGGTGAGGCCTCTGATGAATGATGAAGGCCTGAAG
GTCAGATACCCCCCGAGCCCGGCCAAGATGACCCAGCAC

Protein sequence predicted based on nucleotide sequence

MSKISEAVKRARAAFSSGRTRPLQFRIQQLEALQRLIQEQEQELVGALAADLHKNEWNAY
YEEVVYVLEEIEYMIQKLPEWAADEPVEKTPQTQQDELYIHSEPLGVVLVIGTWNYPFNL
TIQPMVGAIAAGNAVVLKPSELSENMASLLATIIPQYLDKDLYPVINGGVPETTELLKER
FDHILYTGSTGVGKIIMTAAAKHLTPVTLELGGKSPCYVDKNCDLDVACRRIAWGKFMNS
GQTCVAPDYILCDPSIQNQIVEKLKKSLKEFYGEDAKKSRDYGRIISARHFQRVMGLIEG
QKVAYGGTGDAATRYIAPTILTDVDPQSPVMQEEIFGPVLPIVCVRSLEEAIQFINQREK
PLALYMFSSNDKVIKKMIAETSSGGVAANDVIVHITLHSLPFGGVGNSGMGSYHGKKSFE
TFSHRRSCLVRPLMNDEGLKVRYPPSPAKMTQH

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ALDH3A1

Symbol Nomenclature :

ALDH3A1

SYNONYM :

ALDH3; ALDHIII

Designation :

aldehyde dehydrogenase isozyme 3|aldehyde dehydrogenase type III|aldehyde dehydrogenase, dimeric NADP-preferring|stomach aldehyde dehydrogenase

Full Nomenclature :

aldehyde dehydrogenase 3 family, member A1

GENEID :

218

GenBank Accession :

DQ892988

HGNC :

405

MIM :

100660

Vega :

OTTHUMG00000059469

Target GenBank:

DQ892988

Reference Sequence Alignment

  

HsCD00512103        ------------------------------------------------------------
XM_005256525.1      ATGAGCCCGTGGAGAAGACGCCCCAGACTCAGCAGGACGAGCTCTACATCCACTCGGAGC
NM_001135167.1      ------------------------------------------------------------
XM_005256522.1      ATGAGCCCGTGGAGAAGACGCCCCAGACTCAGCAGGACGAGCTCTACATCCACTCGGAGC
XM_005256523.1      ATGAGCCCGTGGAGAAGACGCCCCAGACTCAGCAGGACGAGCTCTACATCCACTCGGAGC
NM_001135168.1      ------------------------------------------------------------
NM_000691.4         ------------------------------------------------------------
                                                                                

HsCD00512103        ------------------------------------------------------------
XM_005256525.1      CACTGGGCGTGGTCCTCGTCATTGGCACCTGGAACTACCCCTTCAACCTCACCATCCAGC
NM_001135167.1      ------------------------------------------------------------
XM_005256522.1      CACTGGGCGTGGTCCTCGTCATTGGCACCTGGAACTACCCCTTCAACCTCACCATCCAGC
XM_005256523.1      CACTGGGCGTGGTCCTCGTCATTGGCACCTGGAACTACCCCTTCAACCTCACCATCCAGC
NM_001135168.1      ------------------------------------------------------------
NM_000691.4         ------------------------------------------------------------
                                                                                

HsCD00512103        ------------------------------------ATGAGCAAGATCAGCGAGGCCGTG
XM_005256525.1      CCATGGTGGGCGCCATCGCTGCAGGTGCATGGCCCTGTGGGAAGGGAAGCTGGGGGAGCA
NM_001135167.1      ------------------------------------ATGAGCAAGATCAGCGAGGCCGTG
XM_005256522.1      CCATGGTGGGCGCCATCGCTGCAGGTGCATGGCCCTGTGGGAAGGGAAGCTGGGGGAGCA
XM_005256523.1      CCATGGTGGGCGCCATCGCTGCAGGTGCATGGCCCTGTGGGAAGGGAAGCTGGGGGAGCA
NM_001135168.1      ------------------------------------ATGAGCAAGATCAGCGAGGCCGTG
NM_000691.4         ------------------------------------ATGAGCAAGATCAGCGAGGCCGTG
                                                        .**.*.*.*.:..  *.** .* .

HsCD00512103        AAGCGCGCCCGCGCCGCCTTCAGCTCGGGCAGGACCCGTCCGCTGCAGTTCCGGATCCAG
XM_005256525.1      CCGTCCCCCAGCGTGGAGCCAAGCCAGGATGGGCTCAGGCCTGTGTTAAGAGTCCTGGGG
NM_001135167.1      AAGCGCGCCCGCGCCGCCTTCAGCTCGGGCAGGACCCGTCCGCTGCAGTTCCGGATCCAG
XM_005256522.1      CCGTCCCCCAGCGTGGAGCCAAGCCAGGATGGGCTCAGGCCTGTGTTAAGAGTCCTGGGG
XM_005256523.1      CCGTCCCCCAGCGTGGAGCCAAGCCAGGATGGGCTCAGGCCTGTGTTAAGAGTCCTGGGG
NM_001135168.1      AAGCGCGCCCGCGCCGCCTTCAGCTCGGGCAGGACCCGTCCGCTGCAGTTCCGGATCCAG
NM_000691.4         AAGCGCGCCCGCGCCGCCTTCAGCTCGGGCAGGACCCGTCCGCTGCAGTTCCGGATCCAG
                    ..*  * **.***  *.   .*** .**. .**. *.* **  ** :.: .   .*  .*

HsCD00512103        CAGCTGGAGGCGCTGCAGCGCCTGATCCAGGAGCAGGAGCAGGAGCTGGTGGGCGCGCTG
XM_005256525.1      TCAGATAAGGGTTTGCTGGGTCTGTCACAGGAGGCTGTGGGGACCAAGCTGTTTAGCCAG
NM_001135167.1      CAGCTGGAGGCGCTGCAGCGCCTGATCCAGGAGCAGGAGCAGGAGCTGGTGGGCGCGCTG
XM_005256522.1      TCAGATAAGGGTTTGCTGGGTCTGTCACAGGAGGCTGTGGGGACCAAGCTGTTTAGCCAG
XM_005256523.1      TCAGATAAGGGTTTGCTGGGTCTGTCACAGGAGGCTGTGGGGACCAAGCTGTTTAGCCAG
NM_001135168.1      CAGCTGGAGGCGCTGCAGCGCCTGATCCAGGAGCAGGAGCAGGAGCTGGTGGGCGCGCTG
NM_000691.4         CAGCTGGAGGCGCTGCAGCGCCTGATCCAGGAGCAGGAGCAGGAGCTGGTGGGCGCGCTG
                     .. : .***   ***:* * ***: .****** . *:* .*.. .:* **   .  *:*

HsCD00512103        GCCGCAG-----------------------------------------------------
XM_005256525.1      GCCTCTTGGTGCCTGAGGTCCAGTCAGGCTCAGGTGTCCACACCTGGTGATACAGAGAGG
NM_001135167.1      GCCGCAG-----------------------------------------------------
XM_005256522.1      GCCTCTTGGTGCCTGAGGTCCAGTCAGGCTCAGGTGTCCACACCTGGTGATACAGAGAGG
XM_005256523.1      GCCTCTTGGTGCCTGAGGTCCAGTCAGGCTCAGGTGTCCACACCTGGTGATACAGAGAGG
NM_001135168.1      GCCGCAG-----------------------------------------------------
NM_000691.4         GCCGCAG-----------------------------------------------------
                    *** *:                                                      

HsCD00512103        ------------------------------------------------------------
XM_005256525.1      GCCTGCCTTCCCCCACCCCTGTTGGGGGGCTGGAGCTTCTGTCACCTAATATGCATCAGG
NM_001135167.1      ------------------------------------------------------------
XM_005256522.1      GCCTGCCTTCCCCCACCCCTGTTGGGGGGCTGGAGCTTCTGTCACCTAATATGCATCAGG
XM_005256523.1      GCCTGCCTTCCCCCACCCCTGTTGGGGGGCTGGAGCTTCTGTCACCTAATATGCATCAGG
NM_001135168.1      ------------------------------------------------------------
NM_000691.4         ------------------------------------------------------------
                                                                                

HsCD00512103        ------------------ACCTGCACAAGAATGAATGGAACGCCTACTATGAGGAGGTGG
XM_005256525.1      GCTTCCTTCCTAGAATGGGCCAGCGCTGTTCTACCAACCGGGCCCCCAGAGCCTCTCTCC
NM_001135167.1      ------------------ACCTGCACAAGAATGAATGGAACGCCTACTATGAGGAGGTGG
XM_005256522.1      GCTTCCTTCCTAGAATGGGCCAGCGCTGTTCTACCAACCGGGCCCCCAGAGCCTCTCTCC
XM_005256523.1      GCTTCCTTCCTAGAATGGGCCAGCGCTGTTCTACCAACCGGGCCCCCAGAGCCTCTCTCC
NM_001135168.1      ------------------ACCTGCACAAGAATGAATGGAACGCCTACTATGAGGAGGTGG
NM_000691.4         ------------------ACCTGCACAAGAATGAATGGAACGCCTACTATGAGGAGGTGG
                                      .**:**.*:. :.*...:. .. *** .*:.:*.  .  *  

HsCD00512103        TGTACGTCCTAGAGGAGATCGAGTACATGATCCAGAAGCTCCCTGAGTGGGCCGCGGATG
XM_005256525.1      CCTTTCTGCTTTTCCTCCAACATCTCAGCAACTCAGAGGGCCAGGTGGTGCAGCTCTGGC
NM_001135167.1      TGTACGTCCTAGAGGAGATCGAGTACATGATCCAGAAGCTCCCTGAGTGGGCCGCGGATG
XM_005256522.1      CCTTTCTGCTTTTCCTCCAACATCTCAGCAACTCAGAGGGCCAGGTGGTGCAGCTCTGGC
XM_005256523.1      CCTTTCTGCTTTTCCTCCAACATCTCAGCAACTCAGAGGGCCAGGTGGTGCAGCTCTGGC
NM_001135168.1      TGTACGTCCTAGAGGAGATCGAGTACATGATCCAGAAGCTCCCTGAGTGGGCCGCGGATG
NM_000691.4         TGTACGTCCTAGAGGAGATCGAGTACATGATCCAGAAGCTCCCTGAGTGGGCCGCGGATG
                      *:  * **: :  : .:. *  :**  *:* ...**  **. *:*  * .     .  

HsCD00512103        AGCCCGTGGAGAAGACGCCCCAGACTCAGCAGGACGAGCTCTACATCCACTCGGAGCCAC
XM_005256525.1      CAGCGTGTGCCCGTGTGTGTCCCTCTGTCATGTGCCCGTGCGTGCTCCTGGGTGTGCCCC
NM_001135167.1      AGCCCGTGGAGAAGACGCCCCAGACTCAGCAGGACGAGCTCTACATCCACTCGGAGCCAC
XM_005256522.1      CAGCGTGTGCCCGTGTGTGTCCCTCTGTCATGTGCCCGTGCGTGCTCCTGGGTGTGCCCC
XM_005256523.1      CAGCGTGTGCCCGTGTGTGTCCCTCTGTCATGTGCCCGTGCGTGCTCCTGGGTGTGCCCC
NM_001135168.1      AGCCCGTGGAGAAGACGCCCCAGACTCAGCAGGACGAGCTCTACATCCACTCGGAGCCAC
NM_000691.4         AGCCCGTGGAGAAGACGCCCCAGACTCAGCAGGACGAGCTCTACATCCACTCGGAGCCAC
                    .. *    *. .. . *   *. :** : .:* .* .*  * : .***:    *:***.*

HsCD00512103        TGGGCGTGGTCCTCGTCATTGGCACCTGGAACTACCCCTTCAACCTCACCATCCAGCCCA
XM_005256525.1      GTGACATGTGCTTGTTTGCTAACAACTCTTCCATCCACTGTCCCGAGGACACACAATGGA
NM_001135167.1      TGGGCGTGGTCCTCGTCATTGGCACCTGGAACTACCCCTTCAACCTCACCATCCAGCCCA
XM_005256522.1      GTGACATGTGCTTGTTTGCTAACAACTCTTCCATCCACTGTCCCGAGGACACACAATGGA
XM_005256523.1      GTGACATGTGCTTGTTTGCTAACAACTCTTCCATCCACTGTCCCGAGGACACACAATGGA
NM_001135168.1      TGGGCGTGGTCCTCGTCATTGGCACCTGGAACTACCCCTTCAACCTCACCATCCAGCCCA
NM_000691.4         TGGGCGTGGTCCTCGTCATTGGCACCTGGAACTACCCCTTCAACCTCACCATCCAGCCCA
                      *.*.**  * *  * . *..**.**  :.*::**.**  ..* : ..** .**.   *

HsCD00512103        TGGTGGGCGCCATCG---------------------------------------------
XM_005256525.1      GGGGAGGCTTCAGGTGTGGTCCTTGGGAGGGCAGAGCCTCTGGTGAAGTACCCTGGGCAT
NM_001135167.1      TGGTGGGCGCCATCG---------------------------------------------
XM_005256522.1      GGGGAGGCTTCAGGTGTGGTCCTTGGGAGGGCAGAGCCTCTGGTGAAGTACCCTGGGCAT
XM_005256523.1      GGGGAGGCTTCAGGTGTGGTCCTTGGGAGGGCAGAGCCTCTGGTGAAGTACCCTGGGCAT
NM_001135168.1      TGGTGGGCGCCATCG---------------------------------------------
NM_000691.4         TGGTGGGCGCCATCG---------------------------------------------
                     ** .***  **                                                

HsCD00512103        -------------------CTGCAGGGAACGCAGTGGTCCTCAAGCCCTCGGAGCTGAGT
XM_005256525.1      AGGCCTGGCTCGGTGTGCTCTGCAGGGAACTCAGTGGTCCTCAAGCCCTCGGAGCTGAGT
NM_001135167.1      -------------------CTGCAGGGAACTCAGTGGTCCTCAAGCCCTCGGAGCTGAGT
XM_005256522.1      AGGCCTGGCTCGGTGTGCTCTGCAGGGAACTCAGTGGTCCTCAAGCCCTCGGAGCTGAGT
XM_005256523.1      AGGCCTGGCTCGGTGTGCTCTGCAGGGAACTCAGTGGTCCTCAAGCCCTCGGAGCTGAGT
NM_001135168.1      -------------------CTGCAGGGAACTCAGTGGTCCTCAAGCCCTCGGAGCTGAGT
NM_000691.4         -------------------CTGCAGGGAACTCAGTGGTCCTCAAGCCCTCGGAGCTGAGT
                                       *********** *****************************

HsCD00512103        GAGAACATGGCGAGCCTGCTGGCTACCATCATCCCCCAGTACCTGGACAAGGATCTGTAC
XM_005256525.1      GAGAACATGGCGAGCCTGCTGGCTACCATCATCCCCCAGTACCTGGACAAGGATCTGTAC
NM_001135167.1      GAGAACATGGCGAGCCTGCTGGCTACCATCATCCCCCAGTACCTGGACAAGGATCTGTAC
XM_005256522.1      GAGAACATGGCGAGCCTGCTGGCTACCATCATCCCCCAGTACCTGGACAAGGATCTGTAC
XM_005256523.1      GAGAACATGGCGAGCCTGCTGGCTACCATCATCCCCCAGTACCTGGACAAGGATCTGTAC
NM_001135168.1      GAGAACATGGCGAGCCTGCTGGCTACCATCATCCCCCAGTACCTGGACAAGGATCTGTAC
NM_000691.4         GAGAACATGGCGAGCCTGCTGGCTACCATCATCCCCCAGTACCTGGACAAGGATCTGTAC
                    ************************************************************

HsCD00512103        CCAGTAATCAATGGGGGTGTCCCTGAGACCACGGAGCTGCTCAAGGAGAGGTTCGACCAT
XM_005256525.1      CCAGTAATCAATGGGGGTGTCCCTGAGACCACGGAGCTGCTCAAGGAGAGGTTCGACCAT
NM_001135167.1      CCAGTAATCAATGGGGGTGTCCCTGAGACCACGGAGCTGCTCAAGGAGAGGTTCGACCAT
XM_005256522.1      CCAGTAATCAATGGGGGTGTCCCTGAGACCACGGAGCTGCTCAAGGAGAGGTTCGACCAT
XM_005256523.1      CCAGTAATCAATGGGGGTGTCCCTGAGACCACGGAGCTGCTCAAGGAGAGGTTCGACCAT
NM_001135168.1      CCAGTAATCAATGGGGGTGTCCCTGAGACCACGGAGCTGCTCAAGGAGAGGTTCGACCAT
NM_000691.4         CCAGTAATCAATGGGGGTGTCCCTGAGACCACGGAGCTGCTCAAGGAGAGGTTCGACCAT
                    ************************************************************

HsCD00512103        ATCCTGTACACGGGCAGCACGGGGGTGGGGAAGATCATCATGACGGCTGCTGCCAAGCAC
XM_005256525.1      ATCCTGTACACGGGCAGCACGGGGGTGGGGAAGATCATCATGACGGCTGCTGCCAAGCAC
NM_001135167.1      ATCCTGTACACGGGCAGCACGGGGGTGGGGAAGATCATCATGACGGCTGCTGCCAAGCAC
XM_005256522.1      ATCCTGTACACGGGCAGCACGGGGGTGGGGAAGATCATCATGACGGCTGCTGCCAAGCAC
XM_005256523.1      ATCCTGTACACGGGCAGCACGGGGGTGGGGAAGATCATCATGACGGCTGCTGCCAAGCAC
NM_001135168.1      ATCCTGTACACGGGCAGCACGGGGGTGGGGAAGATCATCATGACGGCTGCTGCCAAGCAC
NM_000691.4         ATCCTGTACACGGGCAGCACGGGGGTGGGGAAGATCATCATGACGGCTGCTGCCAAGCAC
                    ************************************************************

HsCD00512103        CTGACCCCTGTCACGCTGGAGCTGGGAGGGAAGAGTCCCTGCTACGTGGACAAGAACTGT
XM_005256525.1      CTGACCCCTGTCACGCTGGAGCTGGGAGGGAAGAGTCCCTGCTACGTGGACAAGAACTGT
NM_001135167.1      CTGACCCCTGTCACGCTGGAGCTGGGAGGGAAGAGTCCCTGCTACGTGGACAAGAACTGT
XM_005256522.1      CTGACCCCTGTCACGCTGGAGCTGGGAGGGAAGAGTCCCTGCTACGTGGACAAGAACTGT
XM_005256523.1      CTGACCCCTGTCACGCTGGAGCTGGGAGGGAAGAGTCCCTGCTACGTGGACAAGAACTGT
NM_001135168.1      CTGACCCCTGTCACGCTGGAGCTGGGAGGGAAGAGTCCCTGCTACGTGGACAAGAACTGT
NM_000691.4         CTGACCCCTGTCACGCTGGAGCTGGGAGGGAAGAGTCCCTGCTACGTGGACAAGAACTGT
                    ************************************************************

HsCD00512103        GACCTGGACGTGGCCTGCCGACGCATCGCCTGGGGGAAATTCATGAACAGTGGCCAGACC
XM_005256525.1      GACCTGGACGTGGCCTGCCGACGCATCGCCTGGGGGAAATTCATGAACAGTGGCCAGACC
NM_001135167.1      GACCTGGACGTGGCCTGCCGACGCATCGCCTGGGGGAAATTCATGAACAGTGGCCAGACC
XM_005256522.1      GACCTGGACGTGGCCTGCCGACGCATCGCCTGGGGGAAATTCATGAACAGTGGCCAGACC
XM_005256523.1      GACCTGGACGTGGCCTGCCGACGCATCGCCTGGGGGAAATTCATGAACAGTGGCCAGACC
NM_001135168.1      GACCTGGACGTGGCCTGCCGACGCATCGCCTGGGGGAAATTCATGAACAGTGGCCAGACC
NM_000691.4         GACCTGGACGTGGCCTGCCGACGCATCGCCTGGGGGAAATTCATGAACAGTGGCCAGACC
                    ************************************************************

HsCD00512103        TGCGTGGCCCCAGACTACATCCTCTGTGACCCCTCGATCCAGAACCAAATTGTGGAGAAG
XM_005256525.1      TGCGTGGCCCCTGACTACATCCTCTGTGACCCCTCGATCCAGAACCAAATTGTGGAGAAG
NM_001135167.1      TGCGTGGCCCCTGACTACATCCTCTGTGACCCCTCGATCCAGAACCAAATTGTGGAGAAG
XM_005256522.1      TGCGTGGCCCCTGACTACATCCTCTGTGACCCCTCGATCCAGAACCAAATTGTGGAGAAG
XM_005256523.1      TGCGTGGCCCCTGACTACATCCTCTGTGACCCCTCGATCCAGAACCAAATTGTGGAGAAG
NM_001135168.1      TGCGTGGCCCCTGACTACATCCTCTGTGACCCCTCGATCCAGAACCAAATTGTGGAGAAG
NM_000691.4         TGCGTGGCCCCTGACTACATCCTCTGTGACCCCTCGATCCAGAACCAAATTGTGGAGAAG
                    ***********:************************************************

HsCD00512103        CTCAAGAAGTCACTGAAAGAGTTCTACGGGGAAGATGCTAAGAAATCCCGGGACTATGGA
XM_005256525.1      CTCAAGAAGTCACTGAAAGAGTTCTACGGGGAAGATGCTAAGAAATCCCGGGACTATGGA
NM_001135167.1      CTCAAGAAGTCACTGAAAGAGTTCTACGGGGAAGATGCTAAGAAATCCCGGGACTATGGA
XM_005256522.1      CTCAAGAAGTCACTGAAAGAGTTCTACGGGGAAGATGCTAAGAAATCCCGGGACTATGGA
XM_005256523.1      CTCAAGAAGTCACTGAAAGAGTTCTACGGGGAAGATGCTAAGAAATCCCGGGACTATGGA
NM_001135168.1      CTCAAGAAGTCACTGAAAGAGTTCTACGGGGAAGATGCTAAGAAATCCCGGGACTATGGA
NM_000691.4         CTCAAGAAGTCACTGAAAGAGTTCTACGGGGAAGATGCTAAGAAATCCCGGGACTATGGA
                    ************************************************************

HsCD00512103        AGAATCATTAGTGCCCGGCACTTCCAGAGGGTGATGGGCCTGATTGAGGGCCAGAAGGTG
XM_005256525.1      AGAATCATTAGTGCCCGGCACTTCCAGAGGGTGATGGGCCTGATTGAGGGCCAGAAGGTG
NM_001135167.1      AGAATCATTAGTGCCCGGCACTTCCAGAGGGTGATGGGCCTGATTGAGGGCCAGAAGGTG
XM_005256522.1      AGAATCATTAGTGCCCGGCACTTCCAGAGGGTGATGGGCCTGATTGAGGGCCAGAAGGTG
XM_005256523.1      AGAATCATTAGTGCCCGGCACTTCCAGAGGGTGATGGGCCTGATTGAGGGCCAGAAGGTG
NM_001135168.1      AGAATCATTAGTGCCCGGCACTTCCAGAGGGTGATGGGCCTGATTGAGGGCCAGAAGGTG
NM_000691.4         AGAATCATTAGTGCCCGGCACTTCCAGAGGGTGATGGGCCTGATTGAGGGCCAGAAGGTG
                    ************************************************************

HsCD00512103        GCTTATGGGGGCACCGGGGATGCCGCCACTCGCTACATAGCCCCCACCATCCTCACGGAC
XM_005256525.1      GCTTATGGGGGCACCGGGGATGCCGCCACTCGCTACATAGCCCCCACCATCCTCACGGAC
NM_001135167.1      GCTTATGGGGGCACCGGGGATGCCGCCACTCGCTACATAGCCCCCACCATCCTCACGGAC
XM_005256522.1      GCTTATGGGGGCACCGGGGATGCCGCCACTCGCTACATAGCCCCCACCATCCTCACGGAC
XM_005256523.1      GCTTATGGGGGCACCGGGGATGCCGCCACTCGCTACATAGCCCCCACCATCCTCACGGAC
NM_001135168.1      GCTTATGGGGGCACCGGGGATGCCGCCACTCGCTACATAGCCCCCACCATCCTCACGGAC
NM_000691.4         GCTTATGGGGGCACCGGGGATGCCGCCACTCGCTACATAGCCCCCACCATCCTCACGGAC
                    ************************************************************

HsCD00512103        GTGGACCCCCAGTCCCCGGTGATGCAAGAGGAGATCTTCGGGCCTGTGCTGCCCATCGTG
XM_005256525.1      GTGGACCCCCAGTCCCCGGTGATGCAAGAGGAGATCTTCGGGCCTGTGCTGCCCATCGTG
NM_001135167.1      GTGGACCCCCAGTCCCCGGTGATGCAAGAGGAGATCTTCGGGCCTGTGCTGCCCATCGTG
XM_005256522.1      GTGGACCCCCAGTCCCCGGTGATGCAAGAGGAGATCTTCGGGCCTGTGCTGCCCATCGTG
XM_005256523.1      GTGGACCCCCAGTCCCCGGTGATGCAAGAGGAGATCTTCGGGCCTGTGCTGCCCATCGTG
NM_001135168.1      GTGGACCCCCAGTCCCCGGTGATGCAAGAGGAGATCTTCGGGCCTGTGCTGCCCATCGTG
NM_000691.4         GTGGACCCCCAGTCCCCGGTGATGCAAGAGGAGATCTTCGGGCCTGTGCTGCCCATCGTG
                    ************************************************************

HsCD00512103        TGCGTGCGCAGCCTGGAGGAGGCCATCCAGTTCATCAACCAGCGTGAGAAGCCCCTGGCC
XM_005256525.1      TGCGTGCGCAGCCTGGAGGAGGCCATCCAGTTCATCAACCAGCGTGAGAAGCCCCTGGCC
NM_001135167.1      TGCGTGCGCAGCCTGGAGGAGGCCATCCAGTTCATCAACCAGCGTGAGAAGCCCCTGGCC
XM_005256522.1      TGCGTGCGCAGCCTGGAGGAGGCCATCCAGTTCATCAACCAGCGTGAGAAGCCCCTGGCC
XM_005256523.1      TGCGTGCGCAGCCTGGAGGAGGCCATCCAGTTCATCAACCAGCGTGAGAAGCCCCTGGCC
NM_001135168.1      TGCGTGCGCAGCCTGGAGGAGGCCATCCAGTTCATCAACCAGCGTGAGAAGCCCCTGGCC
NM_000691.4         TGCGTGCGCAGCCTGGAGGAGGCCATCCAGTTCATCAACCAGCGTGAGAAGCCCCTGGCC
                    ************************************************************

HsCD00512103        CTCTACATGTTCTCCAGCAACGACAAGGTGATTAAGAAGATGATTGCAGAGACATCCAGT
XM_005256525.1      CTCTACATGTTCTCCAGCAACGACAAGGTGATTAAGAAGATGATTGCAGAGACATCCAGT
NM_001135167.1      CTCTACATGTTCTCCAGCAACGACAAGGTGATTAAGAAGATGATTGCAGAGACATCCAGT
XM_005256522.1      CTCTACATGTTCTCCAGCAACGACAAGGTGATTAAGAAGATGATTGCAGAGACATCCAGT
XM_005256523.1      CTCTACATGTTCTCCAGCAACGACAAGGTGATTAAGAAGATGATTGCAGAGACATCCAGT
NM_001135168.1      CTCTACATGTTCTCCAGCAACGACAAGGTGATTAAGAAGATGATTGCAGAGACATCCAGT
NM_000691.4         CTCTACATGTTCTCCAGCAACGACAAGGTGATTAAGAAGATGATTGCAGAGACATCCAGT
                    ************************************************************

HsCD00512103        GGTGGGGTGGCGGCCAACGATGTCATCGTCCACATCACCTTGCACTCTCTGCCCTTCGGG
XM_005256525.1      GGTGGGGTGGCGGCCAACGATGTCATCGTCCACATCACCTTGCACTCTCTGCCCTTCGGG
NM_001135167.1      GGTGGGGTGGCGGCCAACGATGTCATCGTCCACATCACCTTGCACTCTCTGCCCTTCGGG
XM_005256522.1      GGTGGGGTGGCGGCCAACGATGTCATCGTCCACATCACCTTGCACTCTCTGCCCTTCGGG
XM_005256523.1      GGTGGGGTGGCGGCCAACGATGTCATCGTCCACATCACCTTGCACTCTCTGCCCTTCGGG
NM_001135168.1      GGTGGGGTGGCGGCCAACGATGTCATCGTCCACATCACCTTGCACTCTCTGCCCTTCGGG
NM_000691.4         GGTGGGGTGGCGGCCAACGATGTCATCGTCCACATCACCTTGCACTCTCTGCCCTTCGGG
                    ************************************************************

HsCD00512103        GGCGTGGGGAACAGCGGCATGGGATCCTACCATGGCAAGAAGAGCTTCGAGACTTTCTCT
XM_005256525.1      GGCGTGGGGAACAGCGGCATGGGATCCTACCATGGCAAGAAGAGCTTCGAGACTTTCTCT
NM_001135167.1      GGCGTGGGGAACAGCGGCATGGGATCCTACCATGGCAAGAAGAGCTTCGAGACTTTCTCT
XM_005256522.1      GGCGTGGGGAACAGCGGCATGGGATCCTACCATGGCAAGAAGAGCTTCGAGACTTTCTCT
XM_005256523.1      GGCGTGGGGAACAGCGGCATGGGATCCTACCATGGCAAGAAGAGCTTCGAGACTTTCTCT
NM_001135168.1      GGCGTGGGGAACAGCGGCATGGGATCCTACCATGGCAAGAAGAGCTTCGAGACTTTCTCT
NM_000691.4         GGCGTGGGGAACAGCGGCATGGGATCCTACCATGGCAAGAAGAGCTTCGAGACTTTCTCT
                    ************************************************************

HsCD00512103        CACCGCCGCTCTTGCCTGGTGAGGCCTCTGATGAATGATGAAGGCCTGAAGGTCAGATAC
XM_005256525.1      CACCGCCGCTCTTGCCTGGTGAGGCCTCTGATGAATGATGAAGGCCTGAAGGTCAGATAC
NM_001135167.1      CACCGCCGCTCTTGCCTGGTGAGGCCTCTGATGAATGATGAAGGCCTGAAGGTCAGATAC
XM_005256522.1      CACCGCCGCTCTTGCCTGGTGAGGCCTCTGATGAATGATGAAGGCCTGAAGGTCAGATAC
XM_005256523.1      CACCGCCGCTCTTGCCTGGTGAGGCCTCTGATGAATGATGAAGGCCTGAAGGTCAGATAC
NM_001135168.1      CACCGCCGCTCTTGCCTGGTGAGGCCTCTGATGAATGATGAAGGCCTGAAGGTCAGATAC
NM_000691.4         CACCGCCGCTCTTGCCTGGTGAGGCCTCTGATGAATGATGAAGGCCTGAAGGTCAGATAC
                    ************************************************************

HsCD00512103        CCCCCGAGCCCGGCCAAGATGACCCAGCAC---
XM_005256525.1      CCCCCGAGCCCGGCCAAGATGACCCAGCACTGA
NM_001135167.1      CCCCCGAGCCCGGCCAAGATGACCCAGCACTGA
XM_005256522.1      CCCCCGAGCCCGGCCAAGATGACCCAGCACTGA
XM_005256523.1      CCCCCGAGCCCGGCCAAGATGACCCAGCACTGA
NM_001135168.1      CCCCCGAGCCCGGCCAAGATGACCCAGCACTGA
NM_000691.4         CCCCCGAGCCCGGCCAAGATGACCCAGCACTGA
                    ******************************