DNASU Plasmid | CHMP7 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: CHMP7

Gene Name: charged multivesicular body protein 7

Original Clone ID:

ccsbBroadEn_04562

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC019110.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512112

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1360nts

Open reading frame: 1 to 1360

Reference Sequence Alignment

ATGTGGTCCCCGGAGCGGGAGGCCGAGGCCCCAGCCGGGGGAGACCCGGCGGGCCTTCTG
CCCCCCGAGTGGGAGGAGGACGAGGAGCGCATGTCCTTCCTGTTCTCCGCTTTCAAGAGG
AGTCGCGAGGTGAACAGCACCGACTGGGACAGCAAGATGGGCTTCTGGGCGCCGTTGGTG
CTGAGCCACAGCCGCCGCCAGGGGGTGGTGCGCCTGCGTCTGCGGGACTTGCAGGAGGCC
TTTCAGCGCAAGGGGAGCGTCCCGCTGGGGCTGGCCACGGTGCTGCAGGACCTGCTGCGT
CGAGGGGAGCTGCAGCGGGAGTCAGACTTCATGGCCAGTGTAGACAGCAGCTGGATCTCC
TGGGGGGTTGGGGTCTTCCTGCTGAAGCCTCTCAAGTGGACTCTTTCTAACATGCTGGGA
GATAATAAGGTTCCAGCTGAGGAGGTCCTTGTCGCTGTGGAGCTGTTGAAGGAAAAGGCT
GAGGAGGTGTATCGTCTGTATCAGAACTCGCCCCTCTCCTCCCACCCCGTGGTGGCCCTG
TCAGAGCTCAGCACCCTCTGTGCTAACTCCTGCCCAGATGAGAGGACCTTCTACTTGGTG
TTGCTGCAGCTGCAGAAGGAGAAGAGGGTCACAGTCCTCGAGCAGAACGGGGAGAAGATT
GTGAAGTTTGCCCGAGGGCCACGTGCCAAGGTCTCTCCAGTCAATGACGTAGATGTTGGG
GTGTACCAGCTGATGCAGAGTGAACAGCTTCTCTCACGCAAAGTGGAGTCCTTATCCCAG
GAAGCAGAGAGGTGTAAAGAAGAAGCCCGCCGGGCATGCCGAGCAGGAAAGAAGCAGCTG
GCACTGAGGTCTCTCAAGGCCAAGCAACGGACAGAGAAGCGCATCGAGGCCTTGCATGCC
AAGCTGGACACTGTTCAAGGCATCCTGGACCGGATCTATGCCTCCCAGACAGATCAGATG
GTTTTTAACGCCTACCAGGCTGGGGTAGGAGCACTCAAACTCTCCATGAAGGATGTCACA
GTGGAGAAGGCAGAGAGCCTCGTGGATCAGATCCAAGAGCTCTGTGACACCCAGGATGAA
GTTTCTCAGACTCTGGCTGGTGGGGTAACAAATGGCTTAGATTTTGACAGTGAAGAACTG
GAGAAGGAATTGGACATCCTCCTTCAGGATACCACCAAAGAACCTTTGGATCTGCCTGAC
AACCCCCGCAATAGGCATTTTACCAACAGCGTGCCTAACCCTAGGATCTCAGATGCTGAA
CTTGAAGCTGAACTTGAGAAACTGTCCTTATCAGAGGGAGGTTTGGTCCCAAGCAGTAAA
TCTCCAAAAAGGCAATTGGAACCGACTCTAAAGCCATTG

Protein sequence predicted based on nucleotide sequence

MWSPEREAEAPAGGDPAGLLPPEWEEDEERMSFLFSAFKRSREVNSTDWDSKMGFWAPLV
LSHSRRQGVVRLRLRDLQEAFQRKGSVPLGLATVLQDLLRRGELQRESDFMASVDSSWIS
WGVGVFLLKPLKWTLSNMLGDNKVPAEEVLVAVELLKEKAEEVYRLYQNSPLSSHPVVAL
SELSTLCANSCPDERTFYLVLLQLQKEKRVTVLEQNGEKIVKFARGPRAKVSPVNDVDVG
VYQLMQSEQLLSRKVESLSQEAERCKEEARRACRAGKKQLALRSLKAKQRTEKRIEALHA
KLDTVQGILDRIYASQTDQMVFNAYQAGVGALKLSMKDVTVEKAESLVDQIQELCDTQDE
VSQTLAGGVTNGLDFDSEELEKELDILLQDTTKEPLDLPDNPRNRHFTNSVPNPRISDAE
LEAELEKLSLSEGGLVPSSKSPKRQLEPTLKPL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

CHMP7

Symbol Nomenclature :

CHMP7

Designation :

CHMP family, member 7|chromatin-modifying protein 7

Full Nomenclature :

charged multivesicular body protein 7

GENEID :

91782

HGNC :

28439

MIM :

611130

Vega :

OTTHUMG00000131785

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00512112      ATGTGGTCCCCGGAGCGGGAGGCCGAGGCCCCAGCCGGGGGAGACCCGGCGGGCCTTCTG
NM_152272.3       ATGTGGTCCCCGGAGCGGGAGGCCGAGGCCCCAGCCGGGGGAGACCCGGCGGGCCTTCTG
                  ************************************************************

HsCD00512112      CCCCCCGAGTGGGAGGAGGACGAGGAGCGCATGTCCTTCCTGTTCTCCGCTTTCAAGAGG
NM_152272.3       CCCCCCGAGTGGGAGGAGGACGAGGAGCGCATGTCCTTCCTGTTCTCCGCTTTCAAGAGG
                  ************************************************************

HsCD00512112      AGTCGCGAGGTGAACAGCACCGACTGGGACAGCAAGATGGGCTTCTGGGCGCCGTTGGTG
NM_152272.3       AGTCGCGAGGTGAACAGCACCGACTGGGACAGCAAGATGGGCTTCTGGGCGCCGTTGGTG
                  ************************************************************

HsCD00512112      CTGAGCCACAGCCGCCGCCAGGGGGTGGTGCGCCTGCGTCTGCGGGACTTGCAGGAGGCC
NM_152272.3       CTGAGCCACAGCCGCCGCCAGGGGGTGGTGCGCCTGCGTCTGCGGGACTTGCAGGAGGCC
                  ************************************************************

HsCD00512112      TTTCAGCGCAAGGGGAGCGTCCCGCTGGGGCTGGCCACGGTGCTGCAGGACCTGCTGCGT
NM_152272.3       TTTCAGCGCAAGGGGAGCGTCCCGCTGGGGCTGGCCACGGTGCTGCAGGACCTGCTGCGT
                  ************************************************************

HsCD00512112      CGAGGGGAGCTGCAGCGGGAGTCAGACTTCATGGCCAGTGTAGACAGCAGCTGGATCTCC
NM_152272.3       CGAGGGGAGCTGCAGCGGGAGTCAGACTTCATGGCCAGTGTAGACAGCAGCTGGATCTCC
                  ************************************************************

HsCD00512112      TGGGGGGTTGGGGTCTTCCTGCTGAAGCCTCTCAAGTGGACTCTTTCTAACATGCTGGGA
NM_152272.3       TGGGGGGTTGGGGTCTTCCTGCTGAAGCCTCTCAAGTGGACTCTTTCTAACATGCTGGGA
                  ************************************************************

HsCD00512112      GATAATAAGGTTCCAGCTGAGGAGGTCCTTGTCGCTGTGGAGCTGTTGAAGGAAAAGGCT
NM_152272.3       GATAATAAGGTTCCAGCTGAGGAGGTCCTTGTCGCTGTGGAGCTGTTGAAGGAAAAGGCT
                  ************************************************************

HsCD00512112      GAGGAGGTGTATCGTCTGTATCAGAACTCGCCCCTCTCCTCCCACCCCGTGGTGGCCCTG
NM_152272.3       GAGGAGGTGTATCGTCTGTATCAGAACTCGCCCCTCTCCTCCCACCCCGTGGTGGCCCTG
                  ************************************************************

HsCD00512112      TCAGAGCTCAGCACCCTCTGTGCTAACTCCTGCCCAGATGAGAGGACCTTCTACTTGGTG
NM_152272.3       TCAGAGCTCAGCACCCTCTGTGCTAACTCCTGCCCAGATGAGAGGACCTTCTACTTGGTG
                  ************************************************************

HsCD00512112      TTGCTGCAGCTGCAGAAGGAGAAGAGGGTCACAGTCCTCGAGCAGAACGGGGAGAAGATT
NM_152272.3       TTGCTGCAGCTGCAGAAGGAGAAGAGGGTCACAGTCCTCGAGCAGAACGGGGAGAAGATT
                  ************************************************************

HsCD00512112      GTGAAGTTTGCCCGAGGGCCACGTGCCAAGGTCTCTCCAGTCAATGACGTAGATGTTGGG
NM_152272.3       GTGAAGTTTGCCCGAGGGCCACGTGCCAAGGTCTCTCCAGTCAATGACGTAGATGTTGGG
                  ************************************************************

HsCD00512112      GTGTACCAGCTGATGCAGAGTGAACAGCTTCTCTCACGCAAAGTGGAGTCCTTATCCCAG
NM_152272.3       GTGTACCAGCTGATGCAGAGTGAACAGCTTCTCTCACGCAAAGTGGAGTCCTTATCCCAG
                  ************************************************************

HsCD00512112      GAAGCAGAGAGGTGTAAAGAAGAAGCCCGCCGGGCATGCCGAGCAGGAAAGAAGCAGCTG
NM_152272.3       GAAGCAGAGAGGTGTAAAGAAGAAGCCCGCCGGGCATGCCGAGCAGGAAAGAAGCAGCTG
                  ************************************************************

HsCD00512112      GCACTGAGGTCTCTCAAGGCCAAGCAACGGACAGAGAAGCGCATCGAGGCCTTGCATGCC
NM_152272.3       GCACTGAGGTCTCTCAAGGCCAAGCAACGGACAGAGAAGCGCATCGAGGCCTTGCATGCC
                  ************************************************************

HsCD00512112      AAGCTGGACACTGTTCAAGGCATCCTGGACCGGATCTATGCCTCCCAGACAGATCAGATG
NM_152272.3       AAGCTGGACACTGTTCAAGGCATCCTGGACCGGATCTATGCCTCCCAGACAGATCAGATG
                  ************************************************************

HsCD00512112      GTTTTTAACGCCTACCAGGCTGGGGTAGGAGCACTCAAACTCTCCATGAAGGATGTCACA
NM_152272.3       GTTTTTAACGCCTACCAGGCTGGGGTAGGAGCACTCAAACTCTCCATGAAGGATGTCACA
                  ************************************************************

HsCD00512112      GTGGAGAAGGCAGAGAGCCTCGTGGATCAGATCCAAGAGCTCTGTGACACCCAGGATGAA
NM_152272.3       GTGGAGAAGGCAGAGAGCCTCGTGGATCAGATCCAAGAGCTCTGTGACACCCAGGATGAA
                  ************************************************************

HsCD00512112      GTTTCTCAGACTCTGGCTGGTGGGGTAACAAATGGCTTAGATTTTGACAGTGAAGAACTG
NM_152272.3       GTTTCTCAGACTCTGGCTGGTGGGGTAACAAATGGCTTAGATTTTGACAGTGAAGAACTG
                  ************************************************************

HsCD00512112      GAGAAGGAATTGGACATCCTCCTTCAGGATACCACCAAAGAACCTTTGGATCTGCCTGAC
NM_152272.3       GAGAAGGAATTGGACATCCTCCTTCAGGATACCACCAAAGAACCTTTGGATCTGCCTGAC
                  ************************************************************

HsCD00512112      AACCCCCGCAATAGGCATTTTACCAACAGCGTGCCTAACCCTAGGATCTCAGATGCTGAA
NM_152272.3       AACCCCCGCAATAGGCATTTTACCAACAGCGTGCCTAACCCTAGGATCTCAGATGCTGAA
                  ************************************************************

HsCD00512112      CTTGAAGCTGAACTTGAGAAACTGTCCTTATCAGAGGGAGGTTTGGTCCCAAGCAGTAAA
NM_152272.3       CTTGAAGCTGAACTTGAGAAACTGTCCTTATCAGAGGGAGGTTTGGTCCCAAGCAGTAAA
                  ************************************************************

HsCD00512112      TCTCCAAAAAGGCAATTGGAACCGACTCTAAAGCCATTG---
NM_152272.3       TCTCCAAAAAGGCAATTGGAACCGACTCTAAAGCCATTGTAG
                  ***************************************

Pathways

Reactome :

Budding and maturation of HIV virion

Reactome :

Disease

Reactome :

Endosomal Sorting Complex Required For Transport (ESCRT)

Reactome :

HIV Infection

Reactome :

HIV Life Cycle

Reactome :

Late Phase of HIV Life Cycle

Reactome :

Membrane Trafficking

Protein

HPRD :

14534

Clone

Cloning Information :

669136

Original Clone ID :

ccsbBroadEn_04562

Organism

Species Specific ID:

91782

Genome

Chromosome :

Map Location :

8p21.3

Ensembl :

ENSG00000147457

Reagents

Labome :

CHMP7-antibody