DNASU Plasmid | OLFM3 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: OLFM3

Gene Name: olfactomedin 3

Original Clone ID:

ccsbBroadEn_09451

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC022531.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512132

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1375nts

Open reading frame: 1 to 1375

Reference Sequence Alignment

ATGCAGGCGACGTCCAACCTTCTCAACCTCCTGCTGCTGTCTTTGTTTGCCGGATTAGAT
CCTTCCAAGACTCAGATTAGTCCTAAAGAAGGGTGGCAGGTGTACAGCTCAGCTCAGGAT
CCTGATGGGCGGTGCATTTGTACAGTTGTTGCTCCAGAACAAAACCTGTGTTCCCGGGAT
GCCAAAAGCAGGCAACTTCGCAAACTACTGGAAAAGGTTCAGAACATGTCCCAGTCTATT
GAAGTCTTAAACTTGAGAACTCAGAGAGATTTCCAATATGTTTTAAAAATGGAAACCCAA
ATGAAAGGGCTGAAGGCAAAATTTCGGCAGATTGAAGATGATCGAAAGACACTTATGACC
AAGCATTTTCAGGAGTTGAAAGAGAAAATGGACGAGCTCCTGCCTTTGATCCCCGTGCTG
GAACAGTACAAAACAGATGCTAAGTTAATCACCCAGTTCAAGGAGGAAATAAGGAATCTG
TCTGCTGTCCTCACTGGTATTCAGGAGGAAATTGGTGCCTATGACTACGAGGAACTACAC
CAAAGAGTGCTGAGCTTGGAAACAAGACTTCGTGACTGCATGAAAAAGCTAACATGTGGC
AAACTGATGAAAATCACAGGCCCAGTTACAGTCAAGACATCTGGAACCCGATTTGGTGCT
TGGATGACAGACCCTTTAGCATCTGAGAAAAACAACAGAGTCTGGTACATGGACAGTTAT
ACTAACAATAAAATTGTTCGTGAATACAAATCAATTGCAGACTTTGTCAGTGGGGCTGAA
TCAAGGACATACAACCTTCCTTTCAAGTGGGCAGGAACTAACCATGTTGTCTACAATGGC
TCACTCTATTTTAACAAGTATCAGAGTAATATCATCATCAAATACAGCTTTGATATGGGG
AGAGTGCTTGCCCAACGAAGCCTGGAGTATGCTGGTTTTCATAATGTTTACCCCTACACA
TGGGGTGGATTCTCTGACATCGACCTAATGGCTGATGAAATCGGGCTGTGGGCTGTGTAT
GCAACTAACCAGAATGCAGGCAATATTGTCATCAGCCAACTTAACCAAGATACCTTGGAG
GTGATGAAGAGCTGGAGCACTGGCTACCCCAAGAGAAGTGCAGGGGAATCTTTCATGATC
TGTGGGACACTGTATGTCACCAACTCCCACTTAACTGGAGCCAAGGTGTATTATTCCTAT
TCCACCAAAACCTCCACATATGAGTACACAGACATTCCCTTCCATAACCAATACTTTCAC
ATATCCATGCTTGACTACAATGCAAGAGATCGAGCTCTCTGTGCCTGGAACAATGGCCAC
CAGGTGCTGTTCAATGTCACCCTTTTCCATATCATCAAGACAGAGGATGACACA

Protein sequence predicted based on nucleotide sequence

MQATSNLLNLLLLSLFAGLDPSKTQISPKEGWQVYSSAQDPDGRCICTVVAPEQNLCSRD
AKSRQLRKLLEKVQNMSQSIEVLNLRTQRDFQYVLKMETQMKGLKAKFRQIEDDRKTLMT
KHFQELKEKMDELLPLIPVLEQYKTDAKLITQFKEEIRNLSAVLTGIQEEIGAYDYEELH
QRVLSLETRLRDCMKKLTCGKLMKITGPVTVKTSGTRFGAWMTDPLASEKNNRVWYMDSY
TNNKIVREYKSIADFVSGAESRTYNLPFKWAGTNHVVYNGSLYFNKYQSNIIIKYSFDMG
RVLAQRSLEYAGFHNVYPYTWGGFSDIDLMADEIGLWAVYATNQNAGNIVISQLNQDTLE
VMKSWSTGYPKRSAGESFMICGTLYVTNSHLTGAKVYYSYSTKTSTYEYTDIPFHNQYFH
ISMLDYNARDRALCAWNNGHQVLFNVTLFHIIKTEDDT

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

OLFM3

Symbol Nomenclature :

OLFM3

SYNONYM :

NOE3; NOELIN3; OPTIMEDIN

Designation :

noelin-3|olfactomedin related ER localized protein 3

Full Nomenclature :

olfactomedin 3

GENEID :

118427

Locus Tag :

RP11-556K13.2

GenBank Accession :

EU176509

HGNC :

17990

MIM :

607567

Vega :

OTTHUMG00000010941

Target GenBank:

EU176509

Reference Sequence Alignment

  

HsCD00512132        ------------------------------------------------------------
NM_058170.3         ------------------------------------------------------------
NM_001288821.1      ATGAGTCCTCCACTGCTGAAGCTTGGCGCTGTGCTTAGTACCATGGCAATGATCTCAAAC
                                                                                

HsCD00512132        ATGCAGGCGACGTCCAACCTTCTCAACCTCCTGCTGCTGTCTTTGTTTGCCGGATTAGAT
NM_058170.3         ATGCAGGCGACGTCCAACCTTCTCAACCTCCTGCTGCTGTCTTTGTTTGCCGGATTAGAT
NM_001288821.1      TGGATGTCCCAAACTCTCCCATCCTTGGTGGGACTGAACACCACGAGGCTGTCGACTCCG
                    : *.:* * ...:* .:** :  *::  *   .***.: :* : *:       .: : . 

HsCD00512132        CCTTCCAAGACTCAGATTAGTCCTAAAGAAGGGTGGCAGGTGTACAGCTCAGCTCAGGAT
NM_058170.3         CCTTCCAAGACTCAGATTAGTCCTAAAGAAGGGTGGCAGGTGTACAGCTCAGCTCAGGAT
NM_001288821.1      GATACCTTAACTCAGATTAGTCCTAAAGAAGGGTGGCAGGTGTACAGCTCAGCTCAGGAT
                     .*:**::.***************************************************

HsCD00512132        CCTGATGGGCGGTGCATTTGTACAGTTGTTGCTCCAGAACAAAACCTGTGTTCCCGGGAT
NM_058170.3         CCTGATGGGCGGTGCATTTGCACAGTTGTTGCTCCAGAACAAAACCTGTGTTCCCGGGAT
NM_001288821.1      CCTGATGGGCGGTGCATTTGCACAGTTGTTGCTCCAGAACAAAACCTGTGTTCCCGGGAT
                    ******************** ***************************************

HsCD00512132        GCCAAAAGCAGGCAACTTCGCAAACTACTGGAAAAGGTTCAGAACATGTCCCAGTCTATT
NM_058170.3         GCCAAAAGCAGGCAACTTCGCCAACTACTGGAAAAGGTTCAGAACATGTCCCAGTCTATT
NM_001288821.1      GCCAAAAGCAGGCAACTTCGCCAACTACTGGAAAAGGTTCAGAACATGTCCCAGTCTATT
                    *********************.**************************************

HsCD00512132        GAAGTCTTAAACTTGAGAACTCAGAGAGATTTCCAATATGTTTTAAAAATGGAAACCCAA
NM_058170.3         GAAGTCTTAAACTTGAGAACTCAGAGAGATTTCCAATATGTTTTAAAAATGGAAACCCAA
NM_001288821.1      GAAGTCTTAAACTTGAGAACTCAGAGAGATTTCCAATATGTTTTAAAAATGGAAACCCAA
                    ************************************************************

HsCD00512132        ATGAAAGGGCTGAAGGCAAAATTTCGGCAGATTGAAGATGATCGAAAGACACTTATGACC
NM_058170.3         ATGAAAGGGCTGAAGGCAAAATTTCGGCAGATTGAAGATGATCGAAAGACACTTATGACC
NM_001288821.1      ATGAAAGGGCTGAAGGCAAAATTTCGGCAGATTGAAGATGATCGAAAGACACTTATGACC
                    ************************************************************

HsCD00512132        AAGCATTTTCAGGAGTTGAAAGAGAAAATGGACGAGCTCCTGCCTTTGATCCCCGTGCTG
NM_058170.3         AAGCATTTTCAGGAGTTGAAAGAGAAAATGGACGAGCTCCTGCCTTTGATCCCCGTGCTG
NM_001288821.1      AAGCATTTTCAGGAGTTGAAAGAGAAAATGGACGAGCTCCTGCCTTTGATCCCCGTGCTG
                    ************************************************************

HsCD00512132        GAACAGTACAAAACAGATGCTAAGTTAATCACCCAGTTCAAGGAGGAAATAAGGAATCTG
NM_058170.3         GAACAGTACAAAACAGATGCTAAGTTAATCACCCAGTTCAAGGAGGAAATAAGGAATCTG
NM_001288821.1      GAACAGTACAAAACAGATGCTAAGTTAATCACCCAGTTCAAGGAGGAAATAAGGAATCTG
                    ************************************************************

HsCD00512132        TCTGCTGTCCTCACTGGTATTCAGGAGGAAATTGGTGCCTATGACTACGAGGAACTACAC
NM_058170.3         TCTGCTGTCCTCACTGGTATTCAGGAGGAAATTGGTGCCTATGACTACGAGGAACTACAC
NM_001288821.1      TCTGCTGTCCTCACTGGTATTCAGGAGGAAATTGGTGCCTATGACTACGAGGAACTACAC
                    ************************************************************

HsCD00512132        CAAAGAGTGCTGAGCTTGGAAACAAGACTTCGTGACTGCATGAAAAAGCTAACATGTGGC
NM_058170.3         CAAAGAGTGCTGAGCTTGGAAACAAGACTTCGTGACTGCATGAAAAAGCTAACATGTGGC
NM_001288821.1      CAAAGAGTGCTGAGCTTGGAAACAAGACTTCGTGACTGCATGAAAAAGCTAACATGTGGC
                    ************************************************************

HsCD00512132        AAACTGATGAAAATCACAGGCCCAGTTACAGTCAAGACATCTGGAACCCGATTTGGTGCT
NM_058170.3         AAACTGATGAAAATCACAGGCCCAGTTACAGTCAAGACATCTGGAACCCGATTTGGTGCT
NM_001288821.1      AAACTGATGAAAATCACAGGCCCAGTTACAGTCAAGACATCTGGAACCCGATTTGGTGCT
                    ************************************************************

HsCD00512132        TGGATGACAGACCCTTTAGCATCTGAGAAAAACAACAGAGTCTGGTACATGGACAGTTAT
NM_058170.3         TGGATGACAGACCCTTTAGCATCTGAGAAAAACAACAGAGTCTGGTACATGGACAGTTAT
NM_001288821.1      TGGATGACAGACCCTTTAGCATCTGAGAAAAACAACAGAGTCTGGTACATGGACAGTTAT
                    ************************************************************

HsCD00512132        ACTAACAATAAAATTGTTCGTGAATACAAATCAATTGCAGACTTTGTCAGTGGGGCTGAA
NM_058170.3         ACTAACAATAAAATTGTTCGTGAATACAAATCAATTGCAGACTTTGTCAGTGGGGCTGAA
NM_001288821.1      ACTAACAATAAAATTGTTCGTGAATACAAATCAATTGCAGACTTTGTCAGTGGGGCTGAA
                    ************************************************************

HsCD00512132        TCAAGGACATACAACCTTCCTTTCAAGTGGGCAGGAACTAACCATGTTGTCTACAATGGC
NM_058170.3         TCAAGGACATACAACCTTCCTTTCAAGTGGGCAGGAACTAACCATGTTGTCTACAATGGC
NM_001288821.1      TCAAGGACATACAACCTTCCTTTCAAGTGGGCAGGAACTAACCATGTTGTCTACAATGGC
                    ************************************************************

HsCD00512132        TCACTCTATTTTAACAAGTATCAGAGTAATATCATCATCAAATACAGCTTTGATATGGGG
NM_058170.3         TCACTCTATTTTAACAAGTATCAGAGTAATATCATCATCAAATACAGCTTTGATATGGGG
NM_001288821.1      TCACTCTATTTTAACAAGTATCAGAGTAATATCATCATCAAATACAGCTTTGATATGGGG
                    ************************************************************

HsCD00512132        AGAGTGCTTGCCCAACGAAGCCTGGAGTATGCTGGTTTTCATAATGTTTACCCCTACACA
NM_058170.3         AGAGTGCTTGCCCAACGAAGCCTGGAGTATGCTGGTTTTCATAATGTTTACCCCTACACA
NM_001288821.1      AGAGTGCTTGCCCAACGAAGCCTGGAGTATGCTGGTTTTCATAATGTTTACCCCTACACA
                    ************************************************************

HsCD00512132        TGGGGTGGATTCTCTGACATCGACCTAATGGCTGATGAAATCGGGCTGTGGGCTGTGTAT
NM_058170.3         TGGGGTGGATTCTCTGACATCGACCTAATGGCTGATGAAATCGGGCTGTGGGCTGTGTAT
NM_001288821.1      TGGGGTGGATTCTCTGACATCGACCTAATGGCTGATGAAATCGGGCTGTGGGCTGTGTAT
                    ************************************************************

HsCD00512132        GCAACTAACCAGAATGCAGGCAATATTGTCATCAGCCAACTTAACCAAGATACCTTGGAG
NM_058170.3         GCAACTAACCAGAATGCAGGCAATATTGTCATCAGCCAACTTAACCAAGATACCTTGGAG
NM_001288821.1      GCAACTAACCAGAATGCAGGCAATATTGTCATCAGCCAACTTAACCAAGATACCTTGGAG
                    ************************************************************

HsCD00512132        GTGATGAAGAGCTGGAGCACTGGCTACCCCAAGAGAAGTGCAGGGGAATCTTTCATGATC
NM_058170.3         GTGATGAAGAGCTGGAGCACTGGCTACCCCAAGAGAAGTGCAGGGGAATCTTTCATGATC
NM_001288821.1      GTGATGAAGAGCTGGAGCACTGGCTACCCCAAGAGAAGTGCAGGGGAATCTTTCATGATC
                    ************************************************************

HsCD00512132        TGTGGGACACTGTATGTCACCAACTCCCACTTAACTGGAGCCAAGGTGTATTATTCCTAT
NM_058170.3         TGTGGGACACTGTATGTCACCAACTCCCACTTAACTGGAGCCAAGGTGTATTATTCCTAT
NM_001288821.1      TGTGGGACACTGTATGTCACCAACTCCCACTTAACTGGAGCCAAGGTGTATTATTCCTAT
                    ************************************************************

HsCD00512132        TCCACCAAAACCTCCACATATGAGTACACAGACATTCCCTTCCATAACCAATACTTTCAC
NM_058170.3         TCCACCAAAACCTCCACATATGAGTACACAGACATTCCCTTCCATAACCAATACTTTCAC
NM_001288821.1      TCCACCAAAACCTCCACATATGAGTACACAGACATTCCCTTCCATAACCAATACTTTCAC
                    ************************************************************

HsCD00512132        ATATCCATGCTTGACTACAATGCAAGAGATCGAGCTCTCTGTGCCTGGAACAATGGCCAC
NM_058170.3         ATATCCATGCTTGACTACAATGCAAGAGATCGAGCTCTCTATGCCTGGAACAATGGCCAC
NM_001288821.1      ATATCCATGCTTGACTACAATGCAAGAGATCGAGCTCTCTATGCCTGGAACAATGGCCAC
                    ****************************************.*******************

HsCD00512132        CAGGTGCTGTTCAATGTCACCCTTTTCCATATCATCAAGACAGAGGATGACACA---
NM_058170.3         CAGGTGCTGTTCAATGTCACCCTTTTCCATATCATCAAGACAGAGGATGACACATAG
NM_001288821.1      CAGGTGCTGTTCAATGTCACCCTTTTCCATATCATCAAGACAGAGGATGACACATAG
                    ******************************************************