DNASU Plasmid | TNFRSF10A (H. sapiens ) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TNFRSF10A

Gene Name: tumor necrosis factor receptor superfamily, member 10a

Original Clone ID:

ccsbBroadEn_07306

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC012866.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512212

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1405nts

Open reading frame: 1 to 1405

Reference Sequence Alignment

ATGGCGCCACCACCAGCTAGAGTACATCTAGGTGCGTTCCTGGCAGTGACTCCGAATCCC
GGGAGCGCAGCGAGTGGGACAGAGGCAGCCGCGGCCACACCCAGCAAAGTGTGGGGCTCT
TCCGCGGGGAGGATTGAACCACGAGGCGGGGGCCGAGGAGCGCTCCCTACCTCCATGGGA
CAGCACGGACCCAGTGCCCGGGCCCGGGCAGGGCGCGCCCCAGGACCCAGGCCGGCGCGG
GAAGCCAGCCCTCGGCTCCGGGTCCACAAGACCTTCAAGTTTGTCGTCGTCGGGGTCCTG
CTGCAGGTCGTACCTAGCTCAGCTGCAACCATCAAACTTCATGATCAATCAATTGGCACA
CAGCAATGGGAACATAGCCCTTTGGGAGAGTTGTGTCCACCAGGATCTCATAGATCAGAA
CATCCTGGAGCCTGTAACCGGTGCACAGAGGGTGTGGGTTACACCAATGCTTCCAACAAT
TTGTTTGCTTGCCTCCCATGTACAGCTTGTAAATCAGATGAAGAAGAGAGAAGTCCCTGC
ACCACGACCAGGAACACAGCATGTCAGTGCAAACCAGGAACTTTCCGGAATGACAATTCT
GCTGAGATGTGCCGGAAGTGCAGCAGAGGGTGCCCCAGAGGGATGGTCAAGGTCAAGGAT
TGTACGCCCTGGAGTGACATCGAGTGTGTCCACAAAGAATCAGGCAATGGACATAATATA
TGGGTGATTTTGGTTGTGACTTTGGTTGTTCCGTTGCTGTTGGTGGCTGTGCTGATTGTC
TGTTGTTGCATCGGCTCAGGTTGTGGAGGGGACCCCAAGTGCATGGACAGGGTGTGTTTC
TGGCGCTTGGGTCTCCTACGAGGGCCTGGGGCTGAGGACAATGCTCACAACGAGATTCTG
AGCAACGCAGACTCGCTGTCCACTTTCGTCTCTGAGCAGCAAATGGAAAGCCAGGAGCCG
GCAGATTTGACAGGTGTCACTGTACAGTCCCCAGGGGAGGCACAGTGTCTGCTGGGACCG
GCAGAAGCTGAAGGGTCTCAGAGGAGGAGGCTGCTGGTTCCAGCAAATGGTGCTGACCCC
ACTGAGACTCTGATGCTGTTCTTTGACAAGTTTGCAAACATCGTGCCCTTTGACTCCTGG
GACCAGCTCATGAGGCAGCTGGACCTCACGAAAAATGAGATCGATGTGGTCAGAGCTGGT
ACAGCAGGCCCAGGGGATGCCTTGTATGCAATGCTGATGAAATGGGTCAACAAAACTGGA
CGGAACGCCTCGATCCACACCCTGCTGGATGCCTTGGAGAGGATGGAAGAGAGACATGCA
AAAGAGAAGATTCAGGACCTCTTGGTGGACTCTGGAAAGTTCATCTACTTAGAAGATGGC
ACAGGCTCTGCCGTGTCCTTGGAG

Protein sequence predicted based on nucleotide sequence

MAPPPARVHLGAFLAVTPNPGSAASGTEAAAATPSKVWGSSAGRIEPRGGGRGALPTSMG
QHGPSARARAGRAPGPRPAREASPRLRVHKTFKFVVVGVLLQVVPSSAATIKLHDQSIGT
QQWEHSPLGELCPPGSHRSEHPGACNRCTEGVGYTNASNNLFACLPCTACKSDEEERSPC
TTTRNTACQCKPGTFRNDNSAEMCRKCSRGCPRGMVKVKDCTPWSDIECVHKESGNGHNI
WVILVVTLVVPLLLVAVLIVCCCIGSGCGGDPKCMDRVCFWRLGLLRGPGAEDNAHNEIL
SNADSLSTFVSEQQMESQEPADLTGVTVQSPGEAQCLLGPAEAEGSQRRRLLVPANGADP
TETLMLFFDKFANIVPFDSWDQLMRQLDLTKNEIDVVRAGTAGPGDALYAMLMKWVNKTG
RNASIHTLLDALERMEERHAKEKIQDLLVDSGKFIYLEDGTGSAVSLE

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

TNFRSF10A

Symbol Nomenclature :

TNFRSF10A

SYNONYM :

APO2; CD261; DR4; TRAILR-1; TRAILR1

Designation :

Full Nomenclature :

tumor necrosis factor receptor superfamily, member 10a

GENEID :

8797

GenBank Accession :

DQ894973

HGNC :

11904

MIM :

603611

Target GenBank:

DQ894973

Reference Sequence Alignment

  

HsCD00512212      ATGGCGCCACCACCAGCTAGAGTACATCTAGGTGCGTTCCTGGCAGTGACTCCGAATCCC
NM_003844.3       ATGGCGCCACCACCAGCTAGAGTACATCTAGGTGCGTTCCTGGCAGTGACTCCGAATCCC
                  ************************************************************

HsCD00512212      GGGAGCGCAGCGAGTGGGACAGAGGCAGCCGCGGCCACACCCAGCAAAGTGTGGGGCTCT
NM_003844.3       GGGAGCGCAGCGAGTGGGACAGAGGCAGCCGCGGCCACACCCAGCAAAGTGTGGGGCTCT
                  ************************************************************

HsCD00512212      TCCGCGGGGAGGATTGAACCACGAGGCGGGGGCCGAGGAGCGCTCCCTACCTCCATGGGA
NM_003844.3       TCCGCGGGGAGGATTGAACCACGAGGCGGGGGCCGAGGAGCGCTCCCTACCTCCATGGGA
                  ************************************************************

HsCD00512212      CAGCACGGACCCAGTGCCCGGGCCCGGGCAGGGCGCGCCCCAGGACCCAGGCCGGCGCGG
NM_003844.3       CAGCACGGACCCAGTGCCCGGGCCCGGGCAGGGCGCGCCCCAGGACCCAGGCCGGCGCGG
                  ************************************************************

HsCD00512212      GAAGCCAGCCCTCGGCTCCGGGTCCACAAGACCTTCAAGTTTGTCGTCGTCGGGGTCCTG
NM_003844.3       GAAGCCAGCCCTCGGCTCCGGGTCCACAAGACCTTCAAGTTTGTCGTCGTCGGGGTCCTG
                  ************************************************************

HsCD00512212      CTGCAGGTCGTACCTAGCTCAGCTGCAACCATCAAACTTCATGATCAATCAATTGGCACA
NM_003844.3       CTGCAGGTCGTACCTAGCTCAGCTGCAACCATCAAACTTCATGATCAATCAATTGGCACA
                  ************************************************************

HsCD00512212      CAGCAATGGGAACATAGCCCTTTGGGAGAGTTGTGTCCACCAGGATCTCATAGATCAGAA
NM_003844.3       CAGCAATGGGAACATAGCCCTTTGGGAGAGTTGTGTCCACCAGGATCTCATAGATCAGAA
                  ************************************************************

HsCD00512212      CATCCTGGAGCCTGTAACCGGTGCACAGAGGGTGTGGGTTACACCAATGCTTCCAACAAT
NM_003844.3       CATCCTGGAGCCTGTAACCGGTGCACAGAGGGTGTGGGTTACACCAATGCTTCCAACAAT
                  ************************************************************

HsCD00512212      TTGTTTGCTTGCCTCCCATGTACAGCTTGTAAATCAGATGAAGAAGAGAGAAGTCCCTGC
NM_003844.3       TTGTTTGCTTGCCTCCCATGTACAGCTTGTAAATCAGATGAAGAAGAGAGAAGTCCCTGC
                  ************************************************************

HsCD00512212      ACCACGACCAGGAACACAGCATGTCAGTGCAAACCAGGAACTTTCCGGAATGACAATTCT
NM_003844.3       ACCACGACCAGGAACACAGCATGTCAGTGCAAACCAGGAACTTTCCGGAATGACAATTCT
                  ************************************************************

HsCD00512212      GCTGAGATGTGCCGGAAGTGCAGCAGAGGGTGCCCCAGAGGGATGGTCAAGGTCAAGGAT
NM_003844.3       GCTGAGATGTGCCGGAAGTGCAGCAGAGGGTGCCCCAGAGGGATGGTCAAGGTCAAGGAT
                  ************************************************************

HsCD00512212      TGTACGCCCTGGAGTGACATCGAGTGTGTCCACAAAGAATCAGGCAATGGACATAATATA
NM_003844.3       TGTACGCCCTGGAGTGACATCGAGTGTGTCCACAAAGAATCAGGCAATGGACATAATATA
                  ************************************************************

HsCD00512212      TGGGTGATTTTGGTTGTGACTTTGGTTGTTCCGTTGCTGTTGGTGGCTGTGCTGATTGTC
NM_003844.3       TGGGTGATTTTGGTTGTGACTTTGGTTGTTCCGTTGCTGTTGGTGGCTGTGCTGATTGTC
                  ************************************************************

HsCD00512212      TGTTGTTGCATCGGCTCAGGTTGTGGAGGGGACCCCAAGTGCATGGACAGGGTGTGTTTC
NM_003844.3       TGTTGTTGCATCGGCTCAGGTTGTGGAGGGGACCCCAAGTGCATGGACAGGGTGTGTTTC
                  ************************************************************

HsCD00512212      TGGCGCTTGGGTCTCCTACGAGGGCCTGGGGCTGAGGACAATGCTCACAACGAGATTCTG
NM_003844.3       TGGCGCTTGGGTCTCCTACGAGGGCCTGGGGCTGAGGACAATGCTCACAACGAGATTCTG
                  ************************************************************

HsCD00512212      AGCAACGCAGACTCGCTGTCCACTTTCGTCTCTGAGCAGCAAATGGAAAGCCAGGAGCCG
NM_003844.3       AGCAACGCAGACTCGCTGTCCACTTTCGTCTCTGAGCAGCAAATGGAAAGCCAGGAGCCG
                  ************************************************************

HsCD00512212      GCAGATTTGACAGGTGTCACTGTACAGTCCCCAGGGGAGGCACAGTGTCTGCTGGGACCG
NM_003844.3       GCAGATTTGACAGGTGTCACTGTACAGTCCCCAGGGGAGGCACAGTGTCTGCTGGGACCG
                  ************************************************************

HsCD00512212      GCAGAAGCTGAAGGGTCTCAGAGGAGGAGGCTGCTGGTTCCAGCAAATGGTGCTGACCCC
NM_003844.3       GCAGAAGCTGAAGGGTCTCAGAGGAGGAGGCTGCTGGTTCCAGCAAATGGTGCTGACCCC
                  ************************************************************

HsCD00512212      ACTGAGACTCTGATGCTGTTCTTTGACAAGTTTGCAAACATCGTGCCCTTTGACTCCTGG
NM_003844.3       ACTGAGACTCTGATGCTGTTCTTTGACAAGTTTGCAAACATCGTGCCCTTTGACTCCTGG
                  ************************************************************

HsCD00512212      GACCAGCTCATGAGGCAGCTGGACCTCACGAAAAATGAGATCGATGTGGTCAGAGCTGGT
NM_003844.3       GACCAGCTCATGAGGCAGCTGGACCTCACGAAAAATGAGATCGATGTGGTCAGAGCTGGT
                  ************************************************************

HsCD00512212      ACAGCAGGCCCAGGGGATGCCTTGTATGCAATGCTGATGAAATGGGTCAACAAAACTGGA
NM_003844.3       ACAGCAGGCCCAGGGGATGCCTTGTATGCAATGCTGATGAAATGGGTCAACAAAACTGGA
                  ************************************************************

HsCD00512212      CGGAACGCCTCGATCCACACCCTGCTGGATGCCTTGGAGAGGATGGAAGAGAGACATGCA
NM_003844.3       CGGAACGCCTCGATCCACACCCTGCTGGATGCCTTGGAGAGGATGGAAGAGAGACATGCA
                  ************************************************************

HsCD00512212      AAAGAGAAGATTCAGGACCTCTTGGTGGACTCTGGAAAGTTCATCTACTTAGAAGATGGC
NM_003844.3       AGAGAGAAGATTCAGGACCTCTTGGTGGACTCTGGAAAGTTCATCTACTTAGAAGATGGC
                  *.**********************************************************

HsCD00512212      ACAGGCTCTGCCGTGTCCTTGGAG---
NM_003844.3       ACAGGCTCTGCCGTGTCCTTGGAGTGA
                  ************************

Pathways

NCI :

Caspase Cascade in Apoptosis

NCI :

Direct p53 effectors

NCI :

TRAIL signaling pathway

Panther :

Apoptosis signaling pathway

Panther :

p53 pathway

WikiPathway :

Apoptosis Modulation and Signaling

WikiPathway :

Lymphocyte TarBase

Protein

SMART domain :

TNFR : Tumor necrosis factor receptor / nerve growth factor receptor repeats.

SMART domain :

DEATH : DEATH domain, found in proteins involved in cell death (apoptosis).

HPRD :

04679

Clone

Cloning Information :

604766

Original Clone ID :

ccsbBroadEn_07306

Organism

Species Specific ID:

8797

Genome

Chromosome :

Map Location :

8p21

Reagents

Labome :

DR4-antibody