DNASU Plasmid | FMO2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: FMO2

Gene Name: flavin containing monooxygenase 2 (non-functional)

Original Clone ID:

ccsbBroadEn_00582

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC005894.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512242

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1414nts

Open reading frame: 1 to 1414

Reference Sequence Alignment

ATGGCAAAGAAGGTAGCTGTGATTGGAGCTGGGGTCAGTGGCCTAATTTCTCTGAAGTGC
TGTGTGGATGAGGGACTTGAGCCCACTTGCTTTGAGAGAACTGAAGATATTGGAGGAGTG
TGGAGGTTCAAAGAGAATGTGGAAGATGGCCGAGCAAGTATCTATCAATCTGTCGTTACC
AACACCAGCAAAGAAATGTCCTGTTTCAGTGACTTTCCAATGCCTGAAGATTTTCCAAAC
TTCCTGCATAATTCTAAACTTCTGGAATATTTCAGGATTTTTGCTAAAAAATTTGATCTG
CTAAAATATATTCAGTTCCAGACAACTGTCCTTAGTGTGAGAAAATGTCCAGATTTCTCA
TCCTCTGGCCAATGGAAGGTTGTCACTCAGAGCAACGGCAAGGAGCAGAGTGCTGTCTTT
GACGCAGTTATGGTTTGCAGTGGCCACCACATTCTACCTCATATCCCACTGAAGTCATTT
CCAGGTATGGAGAGGTTCAAAGGCCAATATTTCCATAGCCGCCAATACAAGCATCCAGAT
GGATTTGAGGGAAAACGCATCCTGGTGATTGGAATGGGAAACTCAGGCTCAGATATTGCT
GTTGAGCTGAGTAAGAATGCTGCTCAGGTTTTTATCAGCACCAGGCATGGCACCTGGGTC
ATGAGCCGTATCTCTGAAGATGGCTATCCTTGGGACTCAGTGTTCCACACCCGGTTTCGT
TCTATGCTCCGCAATGTACTGCCACGAACAGCTGTAAAATGGATGATAGAACAACAGATG
AATCGGTGGTTCAACCATGAAAATTATGGCCTTGAGCCTCAAAACAAATACATTATGAAG
GAACCTGTACTAAATGATGATGTCCCAAGTCGTCTACTCTGTGGAGCCATCAAGGTGAAA
TCTACAGTGAAAGAGCTCACAGAAACTTCTGCCATCTTTGAGGATGGAACAGTGGAGGAG
AACATTGATGTCATCATTTTTGCAACAGGATATAGTTTCTCTTTTCCCTTCCTTGAAGAT
TCACTCGTTAAAGTAGAGAATAATATGGTCTCACTGTATAAATACATATTCCCCGCTCAC
CTGGACAAGTCAACCCTCGCGTGCATTGGTCTCATCCAGCCCCTAGGTTCCATTTTCCCA
ACTGCTGAACTTCAAGCTCGTTGGGTGACAAGAGTTTTCAAAGGCTTGTGTAGCCTGCCC
TCAGAGAGAACTATGATGATGGACATTATCAAAAGGAATGAAAAAAGAATTGACCTGTTT
GGAGAAAGCCAGAGCCAGACGTTGCAGACCAATTATGTTGACTACTTGGACGAGCTCGCC
TTAGAGATAGGTGCGAAGCCAGATTTCTGCTCTCTCTTGTTCAAAGATCCTAAACTGGCT
GTGAGACTCTATTTCGGACCCTGCAACTCCTAT

Protein sequence predicted based on nucleotide sequence

MAKKVAVIGAGVSGLISLKCCVDEGLEPTCFERTEDIGGVWRFKENVEDGRASIYQSVVT
NTSKEMSCFSDFPMPEDFPNFLHNSKLLEYFRIFAKKFDLLKYIQFQTTVLSVRKCPDFS
SSGQWKVVTQSNGKEQSAVFDAVMVCSGHHILPHIPLKSFPGMERFKGQYFHSRQYKHPD
GFEGKRILVIGMGNSGSDIAVELSKNAAQVFISTRHGTWVMSRISEDGYPWDSVFHTRFR
SMLRNVLPRTAVKWMIEQQMNRWFNHENYGLEPQNKYIMKEPVLNDDVPSRLLCGAIKVK
STVKELTETSAIFEDGTVEENIDVIIFATGYSFSFPFLEDSLVKVENNMVSLYKYIFPAH
LDKSTLACIGLIQPLGSIFPTAELQARWVTRVFKGLCSLPSERTMMMDIIKRNEKRIDLF
GESQSQTLQTNYVDYLDELALEIGAKPDFCSLLFKDPKLAVRLYFGPCNSY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

FMO2

Symbol Nomenclature :

FMO2

SYNONYM :

FMO 1B1; FMO 2; FMO1B1

Designation :

FMO, pulmonary|dimethylaniline monooxygenase [N-oxide-forming] 2|dimethylaniline oxidase 2|pulmonary flavin-containing monooxygenase 2

Full Nomenclature :

flavin containing monooxygenase 2 (non-functional)

GENEID :

2327

GenBank Accession :

DQ893748

HGNC :

3770

MIM :

603955

Target GenBank:

DQ893748

Reference Sequence Alignment

  

HsCD00512242      ATGGCAAAGAAGGTAGCTGTGATTGGAGCTGGGGTCAGTGGCCTAATTTCTCTGAAGTGC
NM_001460.3       ATGGCAAAGAAGGTAGCTGTGATTGGAGCTGGGGTCAGTGGCCTAATTTCTCTGAAGTGC
                  ************************************************************

HsCD00512242      TGTGTGGATGAGGGACTTGAGCCCACTTGCTTTGAGAGAACTGAAGATATTGGAGGAGTG
NM_001460.3       TGTGTGGATGAGGGACTTGAGCCCACTTGCTTTGAGAGAACTGAAGATATTGGAGGAGTG
                  ************************************************************

HsCD00512242      TGGAGGTTCAAAGAGAATGTGGAAGATGGCCGAGCAAGTATCTATCAATCTGTCGTTACC
NM_001460.3       TGGAGGTTCAAAGAGAATGTGGAAGATGGCCGAGCAAGTATCTATCAATCTGTCGTTACC
                  ************************************************************

HsCD00512242      AACACCAGCAAAGAAATGTCCTGTTTCAGTGACTTTCCAATGCCTGAAGATTTTCCAAAC
NM_001460.3       AACACCAGCAAAGAAATGTCCTGTTTCAGTGACTTTCCAATGCCTGAAGATTTTCCAAAC
                  ************************************************************

HsCD00512242      TTCCTGCATAATTCTAAACTTCTGGAATATTTCAGGATTTTTGCTAAAAAATTTGATCTG
NM_001460.3       TTCCTGCATAATTCTAAACTTCTGGAATATTTCAGGATTTTTGCTAAAAAATTTGATCTG
                  ************************************************************

HsCD00512242      CTAAAATATATTCAGTTCCAGACAACTGTCCTTAGTGTGAGAAAATGTCCAGATTTCTCA
NM_001460.3       CTAAAATATATTCAGTTCCAGACAACTGTCCTTAGTGTGAGAAAATGTCCAGATTTCTCA
                  ************************************************************

HsCD00512242      TCCTCTGGCCAATGGAAGGTTGTCACTCAGAGCAACGGCAAGGAGCAGAGTGCTGTCTTT
NM_001460.3       TCCTCTGGCCAATGGAAGGTTGTCACTCAGAGCAACGGCAAGGAGCAGAGTGCTGTCTTT
                  ************************************************************

HsCD00512242      GACGCAGTTATGGTTTGCAGTGGCCACCACATTCTACCTCATATCCCACTGAAGTCATTT
NM_001460.3       GACGCAGTTATGGTTTGCAGTGGCCACCACATTCTACCTCATATCCCACTGAAGTCATTT
                  ************************************************************

HsCD00512242      CCAGGTATGGAGAGGTTCAAAGGCCAATATTTCCATAGCCGCCAATACAAGCATCCAGAT
NM_001460.3       CCAGGTATGGAGAGGTTCAAAGGCCAATATTTCCATAGCCGCCAATACAAGCATCCAGAT
                  ************************************************************

HsCD00512242      GGATTTGAGGGAAAACGCATCCTGGTGATTGGAATGGGAAACTCAGGCTCAGATATTGCT
NM_001460.3       GGATTTGAGGGAAAACGCATCCTGGTGATTGGAATGGGAAACTCAGGCTCAGATATTGCT
                  ************************************************************

HsCD00512242      GTTGAGCTGAGTAAGAATGCTGCTCAGGTTTTTATCAGCACCAGGCATGGCACCTGGGTC
NM_001460.3       GTTGAGCTGAGTAAGAATGCTGCTCAGGTTTTTATCAGCACCAGGCATGGCACCTGGGTC
                  ************************************************************

HsCD00512242      ATGAGCCGTATCTCTGAAGATGGCTATCCTTGGGACTCAGTGTTCCACACCCGGTTTCGT
NM_001460.3       ATGAGCCGTATCTCTGAAGATGGCTATCCTTGGGACTCAGTGTTCCACACCCGGTTTCGT
                  ************************************************************

HsCD00512242      TCTATGCTCCGCAATGTACTGCCACGAACAGCTGTAAAATGGATGATAGAACAACAGATG
NM_001460.3       TCTATGCTCCGCAATGTACTGCCACGAACAGCTGTAAAATGGATGATAGAACAACAGATG
                  ************************************************************

HsCD00512242      AATCGGTGGTTCAACCATGAAAATTATGGCCTTGAGCCTCAAAACAAATACATTATGAAG
NM_001460.3       AATCGGTGGTTCAACCATGAAAATTATGGCCTTGAGCCTCAAAACAAATACATTATGAAG
                  ************************************************************

HsCD00512242      GAACCTGTACTAAATGATGATGTCCCAAGTCGTCTACTCTGTGGAGCCATCAAGGTGAAA
NM_001460.3       GAACCTGTACTAAATGATGATGTCCCAAGTCGTCTACTCTGTGGAGCCATCAAGGTGAAA
                  ************************************************************

HsCD00512242      TCTACAGTGAAAGAGCTCACAGAAACTTCTGCCATCTTTGAGGATGGAACAGTGGAGGAG
NM_001460.3       TCTACAGTGAAAGAGCTCACAGAAACTTCTGCCATCTTTGAGGATGGAACAGTGGAGGAG
                  ************************************************************

HsCD00512242      AACATTGATGTCATCATTTTTGCAACAGGATATAGTTTCTCTTTTCCCTTCCTTGAAGAT
NM_001460.3       AACATTGATGTCATCATTTTTGCAACAGGATATAGTTTCTCTTTTCCCTTCCTTGAAGAT
                  ************************************************************

HsCD00512242      TCACTCGTTAAAGTAGAGAATAATATGGTCTCACTGTATAAATACATATTCCCCGCTCAC
NM_001460.3       TCACTCGTTAAAGTAGAGAATAATATGGTCTCACTGTATAAATACATATTCCCCGCTCAC
                  ************************************************************

HsCD00512242      CTGGACAAGTCAACCCTCGCGTGCATTGGTCTCATCCAGCCCCTAGGTTCCATTTTCCCA
NM_001460.3       CTGGACAAGTCAACCCTCGCGTGCATTGGTCTCATCCAGCCCCTAGGTTCCATTTTCCCA
                  ************************************************************

HsCD00512242      ACTGCTGAACTTCAAGCTCGTTGGGTGACAAGAGTTTTCAAAGGCTTGTGTAGCCTGCCC
NM_001460.3       ACTGCTGAACTTCAAGCTCGTTGGGTGACAAGAGTTTTCAAAGGCTTGTGTAGCCTGCCC
                  ************************************************************

HsCD00512242      TCAGAGAGAACTATGATGATGGACATTATCAAAAGGAATGAAAAAAGAATTGACCTGTTT
NM_001460.3       TCAGAGAGAACTATGATGATGGACATTATCAAAAGGAATGAAAAAAGAATTGACCTGTTT
                  ************************************************************

HsCD00512242      GGAGAAAGCCAGAGCCAGACGTTGCAGACCAATTATGTTGACTACTTGGACGAGCTCGCC
NM_001460.3       GGAGAAAGCCAGAGCCAGACGTTGCAGACCAATTATGTTGACTACTTGGACGAGCTCGCC
                  ************************************************************

HsCD00512242      TTAGAGATAGGTGCGAAGCCAGATTTCTGCTCTCTCTTGTTCAAAGATCCTAAACTGGCT
NM_001460.3       TTAGAGATAGGTGCGAAGCCAGATTTCTGCTCTCTCTTGTTCAAAGATCCTAAACTGGCT
                  ************************************************************

HsCD00512242      GTGAGACTCTATTTCGGACCCTGCAACTCCTAT---
NM_001460.3       GTGAGACTCTATTTCGGACCCTGCAACTCCTATTAG
                  *********************************

Pathways

Reactome :

Biological oxidations

Reactome :

FMO oxidizes nucleophiles

Reactome :

Metabolism

Reactome :

Phase 1 - Functionalization of compounds

WikiPathway :

Catalytic cycle of mammalian FMOs

WikiPathway :

metapathway biotransformation

Protein

HPRD :

04903

Clone

Cloning Information :

604796

Original Clone ID :

ccsbBroadEn_00582

Organism

Species Specific ID:

2327

Genome

Chromosome :

Map Location :

1q24.3

Ensembl :

ENSG00000094963

Reagents

Labome :

FMO2-antibody