DNASU Plasmid | TRIP6 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TRIP6

Gene Name: thyroid hormone receptor interactor 6

Original Clone ID:

ccsbBroadEn_01712

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC004999.1,BC028985.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

PMID: 26322679
Title: Proteomic mapping of ER_PM junctions identifies STIMATE as a regulator of Ca2+ influx

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512262

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1429nts

Open reading frame: 1 to 1429

Reference Sequence Alignment

ATGTCGGGGCCCACCTGGCTGCCCCCGAAGCAGCCGGAGCCCGCCAGAGCCCCTCAGGGG
AGGGCGATCCCCCGCGGCACCCCGGGGCCACCACCGGCCCACGGAGCAGCACTCCAGCCC
CACCCCAGGGTCAATTTTTGCCCCCTTCCATCTGAGCAGTGTTACCAGGCCCCAGGGGGA
CCGGAGGATCGGGGGCCGGCGTGGGTGGGGTCCCATGGAGTACTCCAGCACACGCAGGGG
CTCCCTGCAGACAGGGGGGGCCTTCGCCCTGGAAGCCTGGACGCCGAGATAGACTTGCTG
AGCAGCACGCTGGCCGAGCTGAATGGGGGTCGGGGTCATGCGTCACGGCGACCAGACCGA
CAGGCATATGAGCCCCCGCCACCTCCTGCCTACCGCACGGGCTCCCTGAAGCCAAATCCA
GCCTCGCCGCTCCCAGCGTCTCCCTATGGGGGCCCCACTCCAGCCTCTTACACTACCGCC
AGCACCCCGGCTGGCCCAGCCTTCCCCGTGCAAGTGAAGGTGGCACAGCCAGTGAGGGGC
TGCGGCCCACCCAGGCGGGGAGCCTCTCAGGCCTCTGGGCCCCTCCCGGGCCCCCACTTT
CCTCTCCCAGGCCGAGGTGAAGTCTGGGGGCCTGGCTATAGGAGCCAGAGAGAGCCAGGG
CCAGGGGCCAAAGAGGAAGCTGCTGGGGTCTCTGGCCCTGCAGGAAGAGGAAGAGGAGGC
GAGCACGGGCCCCAGGTGCCCCTGAGCCAGCCTCCAGAGGATGAGCTGGATAGGCTGACG
AAGAAGCTGGTTCACGACATGAACCACCCGCCCAGCGGGGAGTACTTTGGCCAGTGTGGT
GGCTGCGGAGAAGATGTGGTTGGGGATGGGGCTGGGGTTGTGGCCCTTGATCGCGTCTTT
CACGTGGGCTGCTTTGTATGTTCTACATGCCGGGCCCAGCTTCGCGGCCAGCATTTCTAC
GCCGTGGAGAGGAGGGCATATTGCGAGGGCTGCTACGTGGCCACCCTGGAGAAATGTGCC
ACGTGCTCCCAGCCCATCCTGGACCGGATCCTGCGGGCTATGGGGAAGGCCTACCACCCT
GGCTGCTTCACCTGCGTGGTGTGTCACCGCGGCCTCGACGGCATCCCCTTCACAGTGGAT
GCTACGAGCCAGATCCACTGCATTGAGGACTTTCACAGGAAGTTTGCCCCAAGATGCTCA
GTGTGCGGTGGGGCCATAATGCCTGAGCCAGGTCAGGAGGAGACTGTGAGAATTGTTGCT
CTGGATCGAAGTTTTCACATTGGCTGTTACAAGTGCGAGGAGTGTGGGCTGCTGCTCTCC
TCTGAGGGCGAGTGTCAGGGCTGCTACCCGCTGGATGGGCACATCTTGTGCAAGGCCTGC
AGCGCCTGGCGCATCCAGGAGCTCTCAGCCACCGTCACCACTGACTGC

Protein sequence predicted based on nucleotide sequence

MSGPTWLPPKQPEPARAPQGRAIPRGTPGPPPAHGAALQPHPRVNFCPLPSEQCYQAPGG
PEDRGPAWVGSHGVLQHTQGLPADRGGLRPGSLDAEIDLLSSTLAELNGGRGHASRRPDR
QAYEPPPPPAYRTGSLKPNPASPLPASPYGGPTPASYTTASTPAGPAFPVQVKVAQPVRG
CGPPRRGASQASGPLPGPHFPLPGRGEVWGPGYRSQREPGPGAKEEAAGVSGPAGRGRGG
EHGPQVPLSQPPEDELDRLTKKLVHDMNHPPSGEYFGQCGGCGEDVVGDGAGVVALDRVF
HVGCFVCSTCRAQLRGQHFYAVERRAYCEGCYVATLEKCATCSQPILDRILRAMGKAYHP
GCFTCVVCHRGLDGIPFTVDATSQIHCIEDFHRKFAPRCSVCGGAIMPEPGQEETVRIVA
LDRSFHIGCYKCEECGLLLSSEGECQGCYPLDGHILCKACSAWRIQELSATVTTDC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

TRIP6

Symbol Nomenclature :

TRIP6

SYNONYM :

OIP-1; OIP1; TRIP-6; TRIP6i2; ZRP-1

Designation :

OPA-interacting protein 1|TR-interacting protein 6|thyroid hormone receptor interacting protein 6|thyroid receptor-interacting protein 6|zyxin related protein 1

Full Nomenclature :

thyroid hormone receptor interactor 6

GENEID :

7205

Locus Tag :

tcag7.1300

GenBank Accession :

DQ896140

HGNC :

12311

MIM :

602933

Vega :

OTTHUMG00000157029

Target GenBank:

DQ896140

Reference Sequence Alignment

  

HsCD00512262      ATGTCGGGGCCCACCTGGCTGCCCCCGAAGCAGCCGGAGCCCGCCAGAGCCCCTCAGGGG
NM_003302.2       ATGTCGGGGCCCACCTGGCTGCCCCCGAAGCAGCCGGAGCCCGCCAGAGCCCCTCAGGGG
                  ************************************************************

HsCD00512262      AGGGCGATCCCCCGCGGCACCCCGGGGCCACCACCGGCCCACGGAGCAGCACTCCAGCCC
NM_003302.2       AGGGCGATCCCCCGCGGCACCCCGGGGCCACCACCGGCCCACGGAGCAGCACTCCAGCCC
                  ************************************************************

HsCD00512262      CACCCCAGGGTCAATTTTTGCCCCCTTCCATCTGAGCAGTGTTACCAGGCCCCAGGGGGA
NM_003302.2       CACCCCAGGGTCAATTTTTGCCCCCTTCCATCTGAGCAGTGTTACCAGGCCCCAGGGGGA
                  ************************************************************

HsCD00512262      CCGGAGGATCGGGGGCCGGCGTGGGTGGGGTCCCATGGAGTACTCCAGCACACGCAGGGG
NM_003302.2       CCGGAGGATCGGGGGCCGGCGTGGGTGGGGTCCCATGGAGTACTCCAGCACACGCAGGGG
                  ************************************************************

HsCD00512262      CTCCCTGCAGACAGGGGGGGCCTTCGCCCTGGAAGCCTGGACGCCGAGATAGACTTGCTG
NM_003302.2       CTCCCTGCAGACAGGGGGGGCCTTCGCCCTGGAAGCCTGGACGCCGAGATAGACTTGCTG
                  ************************************************************

HsCD00512262      AGCAGCACGCTGGCCGAGCTGAATGGGGGTCGGGGTCATGCGTCACGGCGACCAGACCGA
NM_003302.2       AGCAGCACGCTGGCCGAGCTGAATGGGGGTCGGGGTCATGCGTCACGGCGACCAGACCGA
                  ************************************************************

HsCD00512262      CAGGCATATGAGCCCCCGCCACCTCCTGCCTACCGCACGGGCTCCCTGAAGCCAAATCCA
NM_003302.2       CAGGCATATGAGCCCCCGCCACCTCCTGCCTACCGCACGGGCTCCCTGAAGCCAAATCCA
                  ************************************************************

HsCD00512262      GCCTCGCCGCTCCCAGCGTCTCCCTATGGGGGCCCCACTCCAGCCTCTTACACTACCGCC
NM_003302.2       GCCTCGCCGCTCCCAGCGTCTCCCTATGGGGGCCCCACTCCAGCCTCTTACACTACCGCC
                  ************************************************************

HsCD00512262      AGCACCCCGGCTGGCCCAGCCTTCCCCGTGCAAGTGAAGGTGGCACAGCCAGTGAGGGGC
NM_003302.2       AGCACCCCGGCTGGCCCAGCCTTCCCCGTGCAAGTGAAGGTGGCACAGCCAGTGAGGGGC
                  ************************************************************

HsCD00512262      TGCGGCCCACCCAGGCGGGGAGCCTCTCAGGCCTCTGGGCCCCTCCCGGGCCCCCACTTT
NM_003302.2       TGCGGCCCACCCAGGCGGGGAGCCTCTCAGGCCTCTGGGCCCCTCCCGGGCCCCCACTTT
                  ************************************************************

HsCD00512262      CCTCTCCCAGGCCGAGGTGAAGTCTGGGGGCCTGGCTATAGGAGCCAGAGAGAGCCAGGG
NM_003302.2       CCTCTCCCAGGCCGAGGTGAAGTCTGGGGGCCTGGCTATAGGAGCCAGAGAGAGCCAGGG
                  ************************************************************

HsCD00512262      CCAGGGGCCAAAGAGGAAGCTGCTGGGGTCTCTGGCCCTGCAGGAAGAGGAAGAGGAGGC
NM_003302.2       CCAGGGGCCAAAGAGGAAGCTGCTGGGGTCTCTGGCCCTGCAGGAAGAGGAAGAGGAGGC
                  ************************************************************

HsCD00512262      GAGCACGGGCCCCAGGTGCCCCTGAGCCAGCCTCCAGAGGATGAGCTGGATAGGCTGACG
NM_003302.2       GAGCACGGGCCCCAGGTGCCCCTGAGCCAGCCTCCAGAGGATGAGCTGGATAGGCTGACG
                  ************************************************************

HsCD00512262      AAGAAGCTGGTTCACGACATGAACCACCCGCCCAGCGGGGAGTACTTTGGCCAGTGTGGT
NM_003302.2       AAGAAGCTGGTTCACGACATGAACCACCCGCCCAGCGGGGAGTACTTTGGCCAGTGTGGT
                  ************************************************************

HsCD00512262      GGCTGCGGAGAAGATGTGGTTGGGGATGGGGCTGGGGTTGTGGCCCTTGATCGCGTCTTT
NM_003302.2       GGCTGCGGAGAAGATGTGGTTGGGGATGGGGCTGGGGTTGTGGCCCTTGATCGCGTCTTT
                  ************************************************************

HsCD00512262      CACGTGGGCTGCTTTGTATGTTCTACATGCCGGGCCCAGCTTCGCGGCCAGCATTTCTAC
NM_003302.2       CACGTGGGCTGCTTTGTATGTTCTACATGCCGGGCCCAGCTTCGCGGCCAGCATTTCTAC
                  ************************************************************

HsCD00512262      GCCGTGGAGAGGAGGGCATATTGCGAGGGCTGCTACGTGGCCACCCTGGAGAAATGTGCC
NM_003302.2       GCCGTGGAGAGGAGGGCATATTGCGAGGGCTGCTACGTGGCCACCCTGGAGAAATGTGCC
                  ************************************************************

HsCD00512262      ACGTGCTCCCAGCCCATCCTGGACCGGATCCTGCGGGCTATGGGGAAGGCCTACCACCCT
NM_003302.2       ACGTGCTCCCAGCCCATCCTGGACCGGATCCTGCGGGCTATGGGGAAGGCCTACCACCCT
                  ************************************************************

HsCD00512262      GGCTGCTTCACCTGCGTGGTGTGTCACCGCGGCCTCGACGGCATCCCCTTCACAGTGGAT
NM_003302.2       GGCTGCTTCACCTGCGTGGTGTGTCACCGCGGCCTCGACGGCATCCCCTTCACAGTGGAT
                  ************************************************************

HsCD00512262      GCTACGAGCCAGATCCACTGCATTGAGGACTTTCACAGGAAGTTTGCCCCAAGATGCTCA
NM_003302.2       GCTACGAGCCAGATCCACTGCATTGAGGACTTTCACAGGAAGTTTGCCCCAAGATGCTCA
                  ************************************************************

HsCD00512262      GTGTGCGGTGGGGCCATAATGCCTGAGCCAGGTCAGGAGGAGACTGTGAGAATTGTTGCT
NM_003302.2       GTGTGCGGTGGGGCCATAATGCCTGAGCCAGGTCAGGAGGAGACTGTGAGAATTGTTGCT
                  ************************************************************

HsCD00512262      CTGGATCGAAGTTTTCACATTGGCTGTTACAAGTGCGAGGAGTGTGGGCTGCTGCTCTCC
NM_003302.2       CTGGATCGAAGTTTTCACATTGGCTGTTACAAGTGCGAGGAGTGTGGGCTGCTGCTCTCC
                  ************************************************************

HsCD00512262      TCTGAGGGCGAGTGTCAGGGCTGCTACCCGCTGGATGGGCACATCTTGTGCAAGGCCTGC
NM_003302.2       TCTGAGGGCGAGTGTCAGGGCTGCTACCCGCTGGATGGGCACATCTTGTGCAAGGCCTGC
                  ************************************************************

HsCD00512262      AGCGCCTGGCGCATCCAGGAGCTCTCAGCCACCGTCACCACTGACTGC---
NM_003302.2       AGCGCCTGGCGCATCCAGGAGCTCTCAGCCACCGTCACCACTGACTGCTGA
                  ************************************************