DNASU Plasmid | TUBE1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TUBE1

Gene Name: tubulin, epsilon 1

Original Clone ID:

ccsbBroadEn_03236

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC025405.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512305

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1426nts

Open reading frame: 1 to 1426

Reference Sequence Alignment

ATGACCCAGTCGGTGGTCGTACAGGTCGGCCAGTGCGGAAACCAGATCGGCTGCTGCTTC
TGGGACCTGGCACTAAGGGAGCACGCCGCGGTCAACCAGAAAGGAATTTATGATGAGGCA
ATAAGCAGCTTCTTTAGAAATGTGGACACCAGAGTGGTTGGTGATGGTGGAAGTATTTCC
AAGGGAAAAATATGTTCTTTAAAAGCACGAGCAGTCTTGATTGATATGGAAGAAGGGGTA
GTGAATGAAATTCTGCAGGGACCACTGAGAGATGTATTTGATACGAAACAGCTCATCACT
GATATTTCTGGCTCAGGAAATAATTGGGCCGTGGGTCACAAAGTTTTCGGCAGTCTTTAC
CAAGACCAGATTTTAGAGAAATTCAGAAAGTCGGCAGAGCACTGTGATTGCTTGCAGTGT
TTCTTTATAATACATTCCATGGGAGGAGGAACAGGATCTGGACTTGGCACATTTCTTTTA
AAGGTGCTTGAAGACGAATTCCCAGAAGTATACAGATTTGTGACTTCCATTTATCCTTCT
GGTGAGGATGATGTCATAACCTCACCTTATAATAGCATCTTGGCAATGAAGGAACTTAAT
GAGCATGCAGACTGTGTATTGCCCATTGACAATCAATCTTTATTTGACATCATTAGCAAA
ATCGACCTCATGGTGAATTCTGGAAAGTTGGGTACAACTGTGAAGCCAAAGAGTCTGGTT
ACTTCAAGTTCTGGGGCTTTAAAAAAGCAGCATAAGAAGCCCTTTGATGCAATGAATAAC
ATTGTGGCAAATTTGCTCCTCAACCTAACGAGCTCTGCAAGATTTGAAGGGTCCCTTAAT
ATGGACCTTAATGAAATCAGCATGAATTTAGTTCCTTTTCCTCAACTTCATTATCTCGTG
TCAAGCCTAACACCTCTGTATACACTGACAGATGTTAACATTCCTCCTAGAAGATTGGAT
CAGATGTTTTCAGATGCCTTTAGTAAAGATCACCAGCTGCTTCGGGCAGACCCCAAACAC
AGTCTTTACCTCGCCTGTGCACTCATGGTTAGAGGAAATGTACAAATTTCAGATCTTCGT
AGAAATATTGAAAGATTAAAACCATCTCTACAATTTGTCTCCTGGAATCAAGAAGGCTGG
AAGACCAGCCTGTGTTCCGTACCTCCTGTGGGCCATTCTCATTCGTTATTAGCTTTAGCA
AATAACACATGTGTGAAGCCCACCTTCATGGAACTGAAAGAGAGATTCATGAGGCTCTAC
AAGAAAAAGGCTCACCTTCATCACTATCTACAAGTTGAAGGGATGGAAGAAAGCTGTTTC
ACAGAAGCTGTGTCATCTTTATCAGCACTCATACAGGAATATGACCAACTGGACGCCACA
AAAAACATGCCTGTGCAGGATTTACCCAGACTAAGCATAGCTATG

Protein sequence predicted based on nucleotide sequence

MTQSVVVQVGQCGNQIGCCFWDLALREHAAVNQKGIYDEAISSFFRNVDTRVVGDGGSIS
KGKICSLKARAVLIDMEEGVVNEILQGPLRDVFDTKQLITDISGSGNNWAVGHKVFGSLY
QDQILEKFRKSAEHCDCLQCFFIIHSMGGGTGSGLGTFLLKVLEDEFPEVYRFVTSIYPS
GEDDVITSPYNSILAMKELNEHADCVLPIDNQSLFDIISKIDLMVNSGKLGTTVKPKSLV
TSSSGALKKQHKKPFDAMNNIVANLLLNLTSSARFEGSLNMDLNEISMNLVPFPQLHYLV
SSLTPLYTLTDVNIPPRRLDQMFSDAFSKDHQLLRADPKHSLYLACALMVRGNVQISDLR
RNIERLKPSLQFVSWNQEGWKTSLCSVPPVGHSHSLLALANNTCVKPTFMELKERFMRLY
KKKAHLHHYLQVEGMEESCFTEAVSSLSALIQEYDQLDATKNMPVQDLPRLSIAM

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

TUBE1

Symbol Nomenclature :

TUBE1

SYNONYM :

TUBE; dJ142L7.2

Designation :

epsilon-tubulin|tubulin epsilon chain

Full Nomenclature :

tubulin, epsilon 1

GENEID :

51175

Locus Tag :

RP1-142L7.1

GenBank Accession :

DQ894695

HGNC :

20775

MIM :

607345

Vega :

OTTHUMG00000015382

Target GenBank:

DQ894695

Reference Sequence Alignment

  

HsCD00512305      ATGACCCAGTCGGTGGTCGTACAGGTCGGCCAGTGCGGAAACCAGATCGGCTGCTGCTTC
NM_016262.4       ATGACCCAGTCGGTGGTCGTACAGGTCGGCCAGTGCGGAAACCAGATCGGCTGCTGCTTC
                  ************************************************************

HsCD00512305      TGGGACCTGGCACTAAGGGAGCACGCCGCGGTCAACCAGAAAGGAATTTATGATGAGGCA
NM_016262.4       TGGGACCTGGCACTAAGGGAGCACGCCGCGGTCAACCAGAAAGGAATTTATGATGAGGCA
                  ************************************************************

HsCD00512305      ATAAGCAGCTTCTTTAGAAATGTGGACACCAGAGTGGTTGGTGATGGTGGAAGTATTTCC
NM_016262.4       ATAAGCAGCTTCTTTAGAAATGTGGACACCAGAGTGGTTGGTGATGGTGGAAGTATTTCC
                  ************************************************************

HsCD00512305      AAGGGAAAAATATGTTCTTTAAAAGCACGAGCAGTCTTGATTGATATGGAAGAAGGGGTA
NM_016262.4       AAGGGAAAAATATGTTCTTTAAAAGCACGAGCAGTCTTGATTGATATGGAAGAAGGGGTA
                  ************************************************************

HsCD00512305      GTGAATGAAATTCTGCAGGGACCACTGAGAGATGTATTTGATACGAAACAGCTCATCACT
NM_016262.4       GTGAATGAAATTCTGCAGGGACCACTGAGAGATGTATTTGATACGAAACAGCTCATCACT
                  ************************************************************

HsCD00512305      GATATTTCTGGCTCAGGAAATAATTGGGCCGTGGGTCACAAAGTTTTCGGCAGTCTTTAC
NM_016262.4       GATATTTCTGGCTCAGGAAATAATTGGGCCGTGGGTCACAAAGTTTTCGGCAGTCTTTAC
                  ************************************************************

HsCD00512305      CAAGACCAGATTTTAGAGAAATTCAGAAAGTCGGCAGAGCACTGTGATTGCTTGCAGTGT
NM_016262.4       CAAGACCAGATTTTAGAGAAATTCAGAAAGTCGGCAGAGCACTGTGATTGCTTGCAGTGT
                  ************************************************************

HsCD00512305      TTCTTTATAATACATTCCATGGGAGGAGGAACAGGATCTGGACTTGGCACATTTCTTTTA
NM_016262.4       TTCTTTATAATACATTCCATGGGAGGAGGAACAGGATCTGGACTTGGCACATTTCTTTTA
                  ************************************************************

HsCD00512305      AAGGTGCTTGAAGACGAATTCCCAGAAGTATACAGATTTGTGACTTCCATTTATCCTTCT
NM_016262.4       AAGGTGCTTGAAGACGAATTCCCAGAAGTATACAGATTTGTGACTTCCATTTATCCTTCT
                  ************************************************************

HsCD00512305      GGTGAGGATGATGTCATAACCTCACCTTATAATAGCATCTTGGCAATGAAGGAACTTAAT
NM_016262.4       GGTGAGGATGATGTCATAACCTCACCTTATAATAGCATCTTGGCAATGAAGGAACTTAAT
                  ************************************************************

HsCD00512305      GAGCATGCAGACTGTGTATTGCCCATTGACAATCAATCTTTATTTGACATCATTAGCAAA
NM_016262.4       GAGCATGCAGACTGTGTATTGCCCATTGACAATCAATCTTTATTTGACATCATTAGCAAA
                  ************************************************************

HsCD00512305      ATCGACCTCATGGTGAATTCTGGAAAGTTGGGTACAACTGTGAAGCCAAAGAGTCTGGTT
NM_016262.4       ATCGACCTCATGGTGAATTCTGGAAAGTTGGGTACAACTGTGAAGCCAAAGAGTCTGGTT
                  ************************************************************

HsCD00512305      ACTTCAAGTTCTGGGGCTTTAAAAAAGCAGCATAAGAAGCCCTTTGATGCAATGAATAAC
NM_016262.4       ACTTCAAGTTCTGGGGCTTTAAAAAAGCAGCATAAGAAGCCCTTTGATGCAATGAATAAC
                  ************************************************************

HsCD00512305      ATTGTGGCAAATTTGCTCCTCAACCTAACGAGCTCTGCAAGATTTGAAGGGTCCCTTAAT
NM_016262.4       ATTGTGGCAAATTTGCTCCTCAACCTAACGAGCTCTGCAAGATTTGAAGGGTCCCTTAAT
                  ************************************************************

HsCD00512305      ATGGACCTTAATGAAATCAGCATGAATTTAGTTCCTTTTCCTCAACTTCATTATCTCGTG
NM_016262.4       ATGGACCTTAATGAAATCAGCATGAATTTAGTTCCTTTTCCTCAACTTCATTATCTCGTG
                  ************************************************************

HsCD00512305      TCAAGCCTAACACCTCTGTATACACTGACAGATGTTAACATTCCTCCTAGAAGATTGGAT
NM_016262.4       TCAAGCCTAACACCTCTGTATACACTGACAGATGTTAACATTCCTCCTAGAAGATTGGAT
                  ************************************************************

HsCD00512305      CAGATGTTTTCAGATGCCTTTAGTAAAGATCACCAGCTGCTTCGGGCAGACCCCAAACAC
NM_016262.4       CAGATGTTTTCAGATGCCTTTAGTAAAGATCACCAGCTGCTTCGGGCAGACCCCAAACAC
                  ************************************************************

HsCD00512305      AGTCTTTACCTCGCCTGTGCACTCATGGTTAGAGGAAATGTACAAATTTCAGATCTTCGT
NM_016262.4       AGTCTTTACCTCGCCTGTGCACTCATGGTTAGAGGAAATGTACAAATTTCAGATCTTCGT
                  ************************************************************

HsCD00512305      AGAAATATTGAAAGATTAAAACCATCTCTACAATTTGTCTCCTGGAATCAAGAAGGCTGG
NM_016262.4       AGAAATATTGAAAGATTAAAACCATCTCTACAATTTGTCTCCTGGAATCAAGAAGGCTGG
                  ************************************************************

HsCD00512305      AAGACCAGCCTGTGTTCCGTACCTCCTGTGGGCCATTCTCATTCGTTATTAGCTTTAGCA
NM_016262.4       AAGACCAGCCTGTGTTCCGTACCTCCTGTGGGCCATTCTCATTCGTTATTAGCTTTAGCA
                  ************************************************************

HsCD00512305      AATAACACATGTGTGAAGCCCACCTTCATGGAACTGAAAGAGAGATTCATGAGGCTCTAC
NM_016262.4       AATAACACATGTGTGAAGCCCACCTTCATGGAACTGAAAGAGAGATTCATGAGGCTCTAC
                  ************************************************************

HsCD00512305      AAGAAAAAGGCTCACCTTCATCACTATCTACAAGTTGAAGGGATGGAAGAAAGCTGTTTC
NM_016262.4       AAGAAAAAGGCTCACCTTCATCACTATCTACAAGTTGAAGGGATGGAAGAAAGCTGTTTC
                  ************************************************************

HsCD00512305      ACAGAAGCTGTGTCATCTTTATCAGCACTCATACAGGAATATGACCAACTGGACGCCACA
NM_016262.4       ACAGAAGCTGTGTCATCTTTATCAGCACTCATACAGGAATATGACCAACTGGACGCCACA
                  ************************************************************

HsCD00512305      AAAAACATGCCTGTGCAGGATTTACCCAGACTAAGCATAGCTATG---
NM_016262.4       AAAAACATGCCTGTGCAGGATTTACCCAGACTAAGCATAGCTATGTGA
                  *********************************************