DNASU Plasmid | DOK1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: DOK1

Gene Name: docking protein 1, 62kDa (downstream of tyrosine kinase 1)

Original Clone ID:

ccsbBroadEn_00455

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC114440.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

PMID: 26507506
Title: Integrin-beta3 clusters recruit clathrin-mediated endocytic machinery in the absence of traction force

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512351

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1444nts

Open reading frame: 1 to 1444

Reference Sequence Alignment

ATGGACGGAGCAGTGATGGAAGGGCCGCTTTTTTTGCAGAGTCAGCGCTTTGGGACCAAG
AGGTGGAGGAAGACCTGGGCCGTGCTCTACCCGGCCAGTCCCCACGGCGTAGCGCGGCTC
GAGTTCTTTGACCATAAGGGGTCGAGCTCTGGGGGTGGCCGAGGGAGCTCGCGCCGCCTG
GACTGCAAAGTGATCCGTCTGGCTGAGTGTGTGAGTGTGGCCCCCGTCACCGTGGAGACC
CCCCCTGAGCCCGGCGCCACTGCCTTCCGCCTGGACACTGCTCAGCGCTCGCACCTGCTG
GCGGCCGACGCGCCGTCCAGTGCAGCCTGGGTGCAGACGCTGTGCCGAAACGCCTTTCCG
AAAGGCAGCTGGACTCTGGCGCCTACCGATAACCCACCTAAGCTTTCTGCCCTGGAGATG
CTGGAGAACTCCTTGTACAGCCCTACCTGGGAAGGATCCCAATTCTGGGTAACGGTGCAG
AGGACTGAGGCCGCCGAGCGCTGTGGCCTGCATGGCTCCTACGTGCTGAGGGTGGAGGCT
GAAAGGCTGACTCTCCTGACCGTGGGGGCCCAGAGTCAGATACTGGAGCCACTCCTGTCC
TGGCCCTACACTCTGTTGCGTCGCTATGGCCGGGACAAGGTCATGTTCTCTTTCGAGGCC
GGCCGCCGCTGCCCCTCAGGCCCTGGAACCTTCACCTTCCAGACGGCACAGGGAAATGAC
ATCTTCCAGGCAGTTGAGACTGCCATCCACCGGCAGAAGGCCCAGGGAAAGGCCGGACAG
GGGCACGATGTTCTCAGAGCTGACTCCCATGAAGGGGAGGTGGCAGAGGGGAAGTTGCCT
TCCCCACCTGGCCCCCAAGAGCTCCTCGACAGTCCCCCAGCCCTGTATGCTGAGCCCTTA
GACTCCCTGCGCATTGCTCCATGCCCTTCCCAGGACTCCCTATACTCAGACCCCTTGGAC
AGCACGTCTGCTCAGGCAGGAGAGGGAGTACAACGGAAGAAACCTCTCTATTGGGACTTG
TATGAGCATGCGCAGCAGCAGTTGCTGAAGGCCAAGCTGACAGACCCCAAAGAGGATCCC
ATCTATGATGAACCTGAGGGCCTGGCCCCAGTCCCTCCCCAGGGCCTTTATGATCTGCCT
CGGGAGCCCAAGGATGCATGGTGGTGCCAAGCTCGGGTGAAGGAGGAGGGCTATGAGCTC
CCCTACAACCCTGCCACTGATGACTACGCTGTGCCACCCCCTCGGAGCACAAAGCCCCTC
CTTGCTCCCAAGCCCCAGGGCCCAGCCTTCCCTGAACCTGGTACTGCAACTGGCAGTGGC
ATCAAAAGCCACAACTCAGCCCTGTACAGCCAGGTCCAGAAGAGCGGGGCCTCAGGGAGC
TGGGACTGTGGGCTCTCTAGAGTAGGGACTGACAAGACTGGGGTCAAGTCAGAGGGCTCT
ACC

Protein sequence predicted based on nucleotide sequence

MDGAVMEGPLFLQSQRFGTKRWRKTWAVLYPASPHGVARLEFFDHKGSSSGGGRGSSRRL
DCKVIRLAECVSVAPVTVETPPEPGATAFRLDTAQRSHLLAADAPSSAAWVQTLCRNAFP
KGSWTLAPTDNPPKLSALEMLENSLYSPTWEGSQFWVTVQRTEAAERCGLHGSYVLRVEA
ERLTLLTVGAQSQILEPLLSWPYTLLRRYGRDKVMFSFEAGRRCPSGPGTFTFQTAQGND
IFQAVETAIHRQKAQGKAGQGHDVLRADSHEGEVAEGKLPSPPGPQELLDSPPALYAEPL
DSLRIAPCPSQDSLYSDPLDSTSAQAGEGVQRKKPLYWDLYEHAQQQLLKAKLTDPKEDP
IYDEPEGLAPVPPQGLYDLPREPKDAWWCQARVKEEGYELPYNPATDDYAVPPPRSTKPL
LAPKPQGPAFPEPGTATGSGIKSHNSALYSQVQKSGASGSWDCGLSRVGTDKTGVKSEGS
T

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

DOK1

Symbol Nomenclature :

DOK1

SYNONYM :

P62DOK

Designation :

Downstream of tyrosine kinase 1|docking protein 1|docking protein 1 (downstream of tyrosine kinase 1)|p62(dok)|pp62

Full Nomenclature :

docking protein 1, 62kDa (downstream of tyrosine kinase 1)

GENEID :

1796

HGNC :

2990

MIM :

602919

Vega :

OTTHUMG00000129956

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00512351      ATGGACGGAGCAGTGATGGAAGGGCCGCTTTTTTTGCAGAGTCAGCGCTTTGGGACCAAG
NM_001381.3       ATGGACGGAGCAGTGATGGAAGGGCCGCTTTTTTTGCAGAGTCAGCGCTTTGGGACCAAG
                  ************************************************************

HsCD00512351      AGGTGGAGGAAGACCTGGGCCGTGCTCTACCCGGCCAGTCCCCACGGCGTAGCGCGGCTC
NM_001381.3       AGGTGGAGGAAGACCTGGGCCGTGCTCTACCCGGCCAGTCCCCACGGCGTAGCGCGGCTC
                  ************************************************************

HsCD00512351      GAGTTCTTTGACCATAAGGGGTCGAGCTCTGGGGGTGGCCGAGGGAGCTCGCGCCGCCTG
NM_001381.3       GAGTTCTTTGACCATAAGGGGTCGAGCTCTGGGGGTGGCCGAGGGAGCTCGCGCCGCCTG
                  ************************************************************

HsCD00512351      GACTGCAAAGTGATCCGTCTGGCTGAGTGTGTGAGTGTGGCCCCCGTCACCGTGGAGACC
NM_001381.3       GACTGCAAAGTGATCCGTCTGGCTGAGTGTGTGAGTGTGGCCCCCGTCACCGTGGAGACC
                  ************************************************************

HsCD00512351      CCCCCTGAGCCCGGCGCCACTGCCTTCCGCCTGGACACTGCTCAGCGCTCGCACCTGCTG
NM_001381.3       CCCCCTGAGCCCGGCGCCACTGCCTTCCGCCTGGACACTGCTCAGCGCTCGCACCTGCTG
                  ************************************************************

HsCD00512351      GCGGCCGACGCGCCGTCCAGTGCAGCCTGGGTGCAGACGCTGTGCCGAAACGCCTTTCCG
NM_001381.3       GCGGCCGACGCGCCGTCCAGTGCAGCCTGGGTGCAGACGCTGTGCCGAAACGCCTTTCCG
                  ************************************************************

HsCD00512351      AAAGGCAGCTGGACTCTGGCGCCTACCGATAACCCACCTAAGCTTTCTGCCCTGGAGATG
NM_001381.3       AAAGGCAGCTGGACTCTGGCGCCTACCGATAACCCACCTAAGCTTTCTGCCCTGGAGATG
                  ************************************************************

HsCD00512351      CTGGAGAACTCCTTGTACAGCCCTACCTGGGAAGGATCCCAATTCTGGGTAACGGTGCAG
NM_001381.3       CTGGAGAACTCCTTGTACAGCCCTACCTGGGAAGGATCCCAATTCTGGGTAACGGTGCAG
                  ************************************************************

HsCD00512351      AGGACTGAGGCCGCCGAGCGCTGTGGCCTGCATGGCTCCTACGTGCTGAGGGTGGAGGCT
NM_001381.3       AGGACTGAGGCCGCCGAGCGCTGTGGCCTGCATGGCTCCTACGTGCTGAGGGTGGAGGCT
                  ************************************************************

HsCD00512351      GAAAGGCTGACTCTCCTGACCGTGGGGGCCCAGAGTCAGATACTGGAGCCACTCCTGTCC
NM_001381.3       GAAAGGCTGACTCTCCTGACCGTGGGGGCCCAGAGTCAGATACTGGAGCCACTCCTGTCC
                  ************************************************************

HsCD00512351      TGGCCCTACACTCTGTTGCGTCGCTATGGCCGGGACAAGGTCATGTTCTCTTTCGAGGCC
NM_001381.3       TGGCCCTACACTCTGTTGCGTCGCTATGGCCGGGACAAGGTCATGTTCTCTTTCGAGGCC
                  ************************************************************

HsCD00512351      GGCCGCCGCTGCCCCTCAGGCCCTGGAACCTTCACCTTCCAGACGGCACAGGGAAATGAC
NM_001381.3       GGCCGCCGCTGCCCCTCAGGCCCTGGAACCTTCACCTTCCAGACGGCACAGGGAAATGAC
                  ************************************************************

HsCD00512351      ATCTTCCAGGCAGTTGAGACTGCCATCCACCGGCAGAAGGCCCAGGGAAAGGCCGGACAG
NM_001381.3       ATCTTCCAGGCAGTTGAGACTGCCATCCACCGGCAGAAGGCCCAGGGAAAGGCCGGACAG
                  ************************************************************

HsCD00512351      GGGCACGATGTTCTCAGAGCTGACTCCCATGAAGGGGAGGTGGCAGAGGGGAAGTTGCCT
NM_001381.3       GGGCACGATGTTCTCAGAGCTGACTCCCATGAAGGGGAGGTGGCAGAGGGGAAGTTGCCT
                  ************************************************************

HsCD00512351      TCCCCACCTGGCCCCCAAGAGCTCCTCGACAGTCCCCCAGCCCTGTATGCTGAGCCCTTA
NM_001381.3       TCCCCACCTGGCCCCCAAGAGCTCCTCGACAGTCCCCCAGCCCTGTATGCTGAGCCCTTA
                  ************************************************************

HsCD00512351      GACTCCCTGCGCATTGCTCCATGCCCTTCCCAGGACTCCCTATACTCAGACCCCTTGGAC
NM_001381.3       GACTCCCTGCGCATTGCTCCATGCCCTTCCCAGGACTCCCTATACTCAGACCCCTTGGAC
                  ************************************************************

HsCD00512351      AGCACGTCTGCTCAGGCAGGAGAGGGAGTACAACGGAAGAAACCTCTCTATTGGGACTTG
NM_001381.3       AGCACGTCTGCTCAGGCAGGAGAGGGAGTACAACGGAAGAAACCTCTCTATTGGGACTTG
                  ************************************************************

HsCD00512351      TATGAGCATGCGCAGCAGCAGTTGCTGAAGGCCAAGCTGACAGACCCCAAAGAGGATCCC
NM_001381.3       TATGAGCATGCGCAGCAGCAGTTGCTGAAGGCCAAGCTGACAGACCCCAAAGAGGATCCC
                  ************************************************************

HsCD00512351      ATCTATGATGAACCTGAGGGCCTGGCCCCAGTCCCTCCCCAGGGCCTTTATGATCTGCCT
NM_001381.3       ATCTATGATGAACCTGAGGGCCTGGCCCCAGTCCCTCCCCAGGGCCTTTATGATCTGCCT
                  ************************************************************

HsCD00512351      CGGGAGCCCAAGGATGCATGGTGGTGCCAAGCTCGGGTGAAGGAGGAGGGCTATGAGCTC
NM_001381.3       CGGGAGCCCAAGGATGCATGGTGGTGCCAAGCTCGGGTGAAGGAGGAGGGCTATGAGCTC
                  ************************************************************

HsCD00512351      CCCTACAACCCTGCCACTGATGACTACGCTGTGCCACCCCCTCGGAGCACAAAGCCCCTC
NM_001381.3       CCCTACAACCCTGCCACTGATGACTACGCTGTGCCACCCCCTCGGAGCACAAAGCCCCTC
                  ************************************************************

HsCD00512351      CTTGCTCCCAAGCCCCAGGGCCCAGCCTTCCCTGAACCTGGTACTGCAACTGGCAGTGGC
NM_001381.3       CTTGCTCCCAAGCCCCAGGGCCCAGCCTTCCCTGAACCTGGTACTGCAACTGGCAGTGGC
                  ************************************************************

HsCD00512351      ATCAAAAGCCACAACTCAGCCCTGTACAGCCAGGTCCAGAAGAGCGGGGCCTCAGGGAGC
NM_001381.3       ATCAAAAGCCACAACTCAGCCCTGTACAGCCAGGTCCAGAAGAGCGGGGCCTCAGGGAGC
                  ************************************************************

HsCD00512351      TGGGACTGTGGGCTCTCTAGAGTAGGGACTGACAAGACTGGGGTCAAGTCAGAGGGCTCT
NM_001381.3       TGGGACTGTGGGCTCTCTAGAGTAGGGACTGACAAGACTGGGGTCAAGTCAGAGGGCTCT
                  ************************************************************

HsCD00512351      ACC---
NM_001381.3       ACCTGA
                  ***

Pathways

NCI :

BCR signaling pathway

NCI :

Fc-epsilon receptor I signaling in mast cells

NCI :

Insulin Pathway

NCI :

PDGFR-beta signaling pathway

NCI :

Signaling events mediated by PTP1B

NCI :

Signaling events mediated by Stem cell factor receptor (c-Kit)

NCI :

Signaling events regulated by Ret tyrosine kinase

Reactome :

IGF1R signaling cascade

Reactome :

Insulin receptor signalling cascade

Reactome :

IRS activation

Reactome :

IRS-mediated signalling

Reactome :

IRS-related events

Reactome :

IRS-related events triggered by IGF1R

Reactome :

PI3K Cascade

Reactome :

Signal attenuation

Reactome :

Signal Transduction

Reactome :

Signaling by Insulin receptor

Reactome :

Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)

WikiPathway :

Kit receptor signaling pathway

Protein

SMART domain :

PTBI : Phosphotyrosine-binding domain (IRS1-like)

SMART domain :

PH : Pleckstrin homology domain.

HPRD :

04228

Clone

Cloning Information :

669311

Original Clone ID :

ccsbBroadEn_00455

Organism

Species Specific ID:

1796

Genome

Chromosome :

Map Location :

2p13

Ensembl :

ENSG00000115325

Reagents

Labome :

DOK1-antibody