DNASU Plasmid | TRIM39 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TRIM39

Gene Name: tripartite motif containing 39

Original Clone ID:

ccsbBroadEn_03734

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC034985.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512429

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1465nts

Open reading frame: 1 to 1465

Reference Sequence Alignment

ATGGCAGAGACAAGTCTGTTAGAGGCTGGGGCCTCTGCAGCCTCTACAGCTGCGGCTTTG
GAGAACTTACAGGTGGAGGCGAGCTGCTCTGTGTGCCTGGAGTATCTGAAGGAACCTGTC
ATCATTGAGTGTGGGCACAACTTCTGCAAAGCTTGCATCACCCGCTGGTGGGAGGACCTA
GAGAGGGACTTCCCTTGTCCTGTCTGTCGAAAGACATCCCGCTACCGCAGTCTCCGACCT
AATCGGCAACTAGGCAGTATGGTGGAAATTGCCAAGCAGCTCCAGGCCGTCAAGCGGAAG
ATCCGGGATGAGAGCCTCTGCCCCCAACACCATGAGGCCCTCAGCCTTTTCTGTTATGAG
GACCAGGAGGCTGTATGCTTGATATGTGCAATTTCCCACACCCACCGGGCCCACACCGTT
GTGCCACTGGACGATGCTACACAGGAGTACAAGGAAAAACTGCAGAAGTGTCTGGAGCCC
CTGGAACAGAAGCTGCAGGAGATCACTCGCTGCAAGTCCTCTGAGGAGAAGAAGCCTGGT
GAGCTCAAGAGACTAGTGGAAAGTCGCCGACAGCAGATCTTGAGGGAGTTTGAAGAGCTT
CATAGGCGGCTGGATGAAGAGCAGCAGGTGTTGCTTTCACGACTGGAAGAAGAGGAACAG
GACATTCTGCAGCGACTCCGAGAAAATGCTGCTCACCTTGGGGACAAGCGCCGGGACCTG
GCCCACTTGGCTGCCGAGGTGGAGGGCAAGTGCTTACAGTCAGGCTTCGAGATGCTTAAG
GATGTCAAAAGTACCCTGGAAAAATGTGAAAAGGTGAAGACCATGGAGGTGACTTCAGTA
TCCATAGAGCTGGAAAAGAACTTCAGCAATTTTCCCCGACAGTACTTTGCCCTAAGGAAA
ATCCTTAAACAGCTAATTGCGGATGTGACCCTGGACCCTGAGACAGCTCATCCTAACCTA
GTCCTGTCAGAGGATCGTAAGAGCGTCAAGTTCGTGGAGACAAGACTCCGGGATCTCCCT
GACACACCAAGGCGTTTCACCTTCTACCCTTGCGTCCTGGCTACTGAGGGTTTCACCTCA
GGTCGACACTACTGGGAGGTGGAGGTGGGCGACAAGACCCACTGGGCAGTGGGTGTATGC
CGGGACTCCGTGAGCCGAAAGGGCGAGTTGACTCCACTCCCTGAGACTGGCTACTGGCGG
GTGCGGCTATGGAATGGGGACAAATATGCAGCCACCACCACACCTTTTACCCCTTTGCAC
ATCAAGGTGAAACCCAAGCGGGTAGGCATATTCCTAGACTATGAGGCCGGCACACTGTCT
TTCTACAATGTCACAGACCGCTCTCATATCTACACCTTCACTGATACTTTTACTGAGAAA
CTTTGGCCCCTCTTCTACCCAGGCATCCGGGCTGGACGGAAGAATGCTGCACCACTTACC
ATCAGGCCCCCAACAGATTGGGAG

Protein sequence predicted based on nucleotide sequence

MAETSLLEAGASAASTAAALENLQVEASCSVCLEYLKEPVIIECGHNFCKACITRWWEDL
ERDFPCPVCRKTSRYRSLRPNRQLGSMVEIAKQLQAVKRKIRDESLCPQHHEALSLFCYE
DQEAVCLICAISHTHRAHTVVPLDDATQEYKEKLQKCLEPLEQKLQEITRCKSSEEKKPG
ELKRLVESRRQQILREFEELHRRLDEEQQVLLSRLEEEEQDILQRLRENAAHLGDKRRDL
AHLAAEVEGKCLQSGFEMLKDVKSTLEKCEKVKTMEVTSVSIELEKNFSNFPRQYFALRK
ILKQLIADVTLDPETAHPNLVLSEDRKSVKFVETRLRDLPDTPRRFTFYPCVLATEGFTS
GRHYWEVEVGDKTHWAVGVCRDSVSRKGELTPLPETGYWRVRLWNGDKYAATTTPFTPLH
IKVKPKRVGIFLDYEAGTLSFYNVTDRSHIYTFTDTFTEKLWPLFYPGIRAGRKNAAPLT
IRPPTDWE

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

TRIM39

Symbol Nomenclature :

TRIM39

SYNONYM :

RNF23; TFP; TRIM39B

Designation :

E3 ubiquitin-protein ligase TRIM39|ring finger protein 23|testis-abundant finger protein|tripartite motif-containing 39|tripartite motif-containing protein 39

Full Nomenclature :

tripartite motif containing 39

GENEID :

56658

Locus Tag :

DAAP-385L22.4

HGNC :

10065

MIM :

605700

Vega :

OTTHUMG00000031066

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00512429      ATGGCAGAGACAAGTCTGTTAGAGGCTGGGGCCTCTGCAGCCTCTACAGCTGCGGCTTTG
NM_021253.3       ATGGCAGAGACAAGTCTGTTAGAGGCTGGGGCCTCTGCAGCCTCTACAGCTGCGGCTTTG
NM_172016.2       ATGGCAGAGACAAGTCTGTTAGAGGCTGGGGCCTCTGCAGCCTCTACAGCTGCGGCTTTG
                  ************************************************************

HsCD00512429      GAGAACTTACAGGTGGAGGCGAGCTGCTCTGTGTGCCTGGAGTATCTGAAGGAACCTGTC
NM_021253.3       GAGAACTTACAGGTGGAGGCGAGCTGCTCTGTGTGCCTGGAGTATCTGAAGGAACCTGTC
NM_172016.2       GAGAACTTACAGGTGGAGGCGAGCTGCTCTGTGTGCCTGGAGTATCTGAAGGAACCTGTC
                  ************************************************************

HsCD00512429      ATCATTGAGTGTGGGCACAACTTCTGCAAAGCTTGCATCACCCGCTGGTGGGAGGACCTA
NM_021253.3       ATCATTGAGTGTGGGCACAACTTCTGCAAAGCTTGCATCACCCGCTGGTGGGAGGACCTA
NM_172016.2       ATCATTGAGTGTGGGCACAACTTCTGCAAAGCTTGCATCACCCGCTGGTGGGAGGACCTA
                  ************************************************************

HsCD00512429      GAGAGGGACTTCCCTTGTCCTGTCTGTCGAAAGACATCCCGCTACCGCAGTCTCCGACCT
NM_021253.3       GAGAGGGACTTCCCTTGTCCTGTCTGTCGAAAGACATCCCGCTACCGCAGTCTCCGACCT
NM_172016.2       GAGAGGGACTTCCCTTGTCCTGTCTGTCGAAAGACATCCCGCTACCGCAGTCTCCGACCT
                  ************************************************************

HsCD00512429      AATCGGCAACTAGGCAGTATGGTGGAAATTGCCAAGCAGCTCCAGGCCGTCAAGCGGAAG
NM_021253.3       AATCGGCAACTAGGCAGTATGGTGGAAATTGCCAAGCAGCTCCAGGCCGTCAAGCGGAAG
NM_172016.2       AATCGGCAACTAGGCAGTATGGTGGAAATTGCCAAGCAGCTCCAGGCCGTCAAGCGGAAG
                  ************************************************************

HsCD00512429      ATCCGGGATGAGAGCCTCTGCCCCCAACACCATGAGGCCCTCAGCCTTTTCTGTTATGAG
NM_021253.3       ATCCGGGATGAGAGCCTCTGCCCCCAACACCATGAGGCCCTCAGCCTTTTCTGTTATGAG
NM_172016.2       ATCCGGGATGAGAGCCTCTGCCCCCAACACCATGAGGCCCTCAGCCTTTTCTGTTATGAG
                  ************************************************************

HsCD00512429      GACCAGGAGGCTGTATGCTTGATATGTGCAATTTCCCACACCCACCGGGCCCACACCGTT
NM_021253.3       GACCAGGAGGCTGTATGCTTGATATGTGCAATTTCCCACACCCACCGGGCCCACACCGTT
NM_172016.2       GACCAGGAGGCTGTATGCTTGATATGTGCAATTTCCCACACCCACCGGGCCCACACCGTT
                  ************************************************************

HsCD00512429      GTGCCACTGGACGATGCTACACAGGAGTACAAGGAAAAACTGCAGAAGTGTCTGGAGCCC
NM_021253.3       GTGCCACTGGACGATGCTACACAGGAGTACAAGGAAAAACTGCAGAAGTGTCTGGAGCCC
NM_172016.2       GTGCCACTGGACGATGCTACACAGGAGTACAAGGAAAAACTGCAGAAGTGTCTGGAGCCC
                  ************************************************************

HsCD00512429      CTGGAACAGAAGCTGCAGGAGATCACTCGCTGCAAGTCCTCTGAGGAGAAGAAGCCTGGT
NM_021253.3       CTGGAACAGAAGCTGCAGGAGATCACTCGCTGCAAGTCCTCTGAGGAGAAGAAGCCTGGT
NM_172016.2       CTGGAACAGAAGCTGCAGGAGATCACTCGCTGCAAGTCCTCTGAGGAGAAGAAGCCTGGT
                  ************************************************************

HsCD00512429      GAGCTCAAGAGACTAGTGGAAAGTCGCCGACAGCAGATCTTGAGGGAGTTTGAAGAGCTT
NM_021253.3       GAGCTCAAGAGACTAGTGGAAAGTCGCCGACAGCAGATCTTGAGGGAGTTTGAAGAGCTT
NM_172016.2       GAGCTCAAGAGACTAGTGGAAAGTCGCCGACAGCAGATCTTGAGGGAGTTTGAAGAGCTT
                  ************************************************************

HsCD00512429      CATAGGCGGCTGGATGAAGAGCAGCAGGTGTTGCTTTCACGACTGGAAGAAGAGGAACAG
NM_021253.3       CATAGGCGGCTGGATGAAGAGCAGCAGGTGTTGCTTTCACGACTGGAAGAAGAGGAACAG
NM_172016.2       CATAGGCGGCTGGATGAAGAGCAGCAGGTGTTGCTTTCACGACTGGAAGAAGAGGAACAG
                  ************************************************************

HsCD00512429      GACATTCTGCAGCGACTCCGAGAAAATGCTGCTCACCTTGGGGACAAGCGCCGGGACCTG
NM_021253.3       GACATTCTGCAGCGACTCCGAGAAAATGCTGCTCACCTTGGGGACAAGCGCCGGGACCTG
NM_172016.2       GACATTCTGCAGCGACTCCGAGAAAATGCTGCTCACCTTGGGGACAAGCGCCGGGACCTG
                  ************************************************************

HsCD00512429      GCCCACTTGGCTGCCGAGGTGGAGGGCAAGTGCTTACAGTCAGGCTTCGAGATGCTTAAG
NM_021253.3       GCCCACTTGGCTGCCGAGGTGGAGGGCAAGTGCTTACAGTCAGGCTTCGAGATGCTTAAG
NM_172016.2       GCCCACTTGGCTGCCGAGGTGGAGGGCAAGTGCTTACAGTCAGGCTTCGAGATGCTTAAG
                  ************************************************************

HsCD00512429      GATGTCAAAAGTACCCTG------------------------------------------
NM_021253.3       GATGTCAAAAGTACCCTGGAAAAGAATATTCCTAGAAAGTTCGGAGGCTCACTCTCAACG
NM_172016.2       GATGTCAAAAGTACCCTG------------------------------------------
                  ******************                                          

HsCD00512429      ------------------------------------------------GAAAAATGTGAA
NM_021253.3       ATCTGTCCACGGGATCATAAGGCTCTCCTTGGATTAGTAAAAGAAATCAACAGATGTGAA
NM_172016.2       ------------------------------------------------GAAAAATGTGAA
                                                                  .*.*.*******

HsCD00512429      AAGGTGAAGACCATGGAGGTGACTTCAGTATCCATAGAGCTGGAAAAGAACTTCAGCAAT
NM_021253.3       AAGGTGAAGACCATGGAGGTGACTTCAGTATCCATAGAGCTGGAAAAGAACTTCAGCAAT
NM_172016.2       AAGGTGAAGACCATGGAGGTGACTTCAGTATCCATAGAGCTGGAAAAGAACTTCAGCAAT
                  ************************************************************

HsCD00512429      TTTCCCCGACAGTACTTTGCCCTAAGGAAAATCCTTAAACAGCTAATTGCGGATGTGACC
NM_021253.3       TTTCCCCGACAGTACTTTGCCCTAAGGAAAATCCTTAAACAGCTAATTGCGGATGTGACC
NM_172016.2       TTTCCCCGACAGTACTTTGCCCTAAGGAAAATCCTTAAACAGCTAATTGCGGATGTGACC
                  ************************************************************

HsCD00512429      CTGGACCCTGAGACAGCTCATCCTAACCTAGTCCTGTCAGAGGATCGTAAGAGCGTCAAG
NM_021253.3       CTGGACCCTGAGACAGCTCATCCTAACCTAGTCCTGTCAGAGGATCGTAAGAGCGTCAAG
NM_172016.2       CTGGACCCTGAGACAGCTCATCCTAACCTAGTCCTGTCAGAGGATCGTAAGAGCGTCAAG
                  ************************************************************

HsCD00512429      TTCGTGGAGACAAGACTCCGGGATCTCCCTGACACACCAAGGCGTTTCACCTTCTACCCT
NM_021253.3       TTCGTGGAGACAAGACTCCGGGATCTCCCTGACACACCAAGGCGTTTCACCTTCTACCCT
NM_172016.2       TTCGTGGAGACAAGACTCCGGGATCTCCCTGACACACCAAGGCGTTTCACCTTCTACCCT
                  ************************************************************

HsCD00512429      TGCGTCCTGGCTACTGAGGGTTTCACCTCAGGTCGACACTACTGGGAGGTGGAGGTGGGC
NM_021253.3       TGCGTCCTGGCTACTGAGGGTTTCACCTCAGGTCGACACTACTGGGAGGTGGAGGTGGGC
NM_172016.2       TGCGTCCTGGCTACTGAGGGTTTCACCTCAGGTCGACACTACTGGGAGGTGGAGGTGGGC
                  ************************************************************

HsCD00512429      GACAAGACCCACTGGGCAGTGGGTGTATGCCGGGACTCCGTGAGCCGAAAGGGCGAGTTG
NM_021253.3       GACAAGACCCACTGGGCAGTGGGTGTATGCCGGGACTCCGTGAGCCGAAAGGGCGAGTTG
NM_172016.2       GACAAGACCCACTGGGCAGTGGGTGTATGCCGGGACTCCGTGAGCCGAAAGGGCGAGTTG
                  ************************************************************

HsCD00512429      ACTCCACTCCCTGAGACTGGCTACTGGCGGGTGCGGCTATGGAATGGGGACAAATATGCA
NM_021253.3       ACTCCACTCCCTGAGACTGGCTACTGGCGGGTGCGGCTATGGAATGGGGACAAATATGCA
NM_172016.2       ACTCCACTCCCTGAGACTGGCTACTGGCGGGTGCGGCTATGGAATGGGGACAAATATGCA
                  ************************************************************

HsCD00512429      GCCACCACCACACCTTTTACCCCTTTGCACATCAAGGTGAAACCCAAGCGGGTAGGCATA
NM_021253.3       GCCACCACCACACCTTTTACCCCTTTGCACATCAAGGTGAAACCCAAGCGGGTAGGCATA
NM_172016.2       GCCACCACCACACCTTTTACCCCTTTGCACATCAAGGTGAAACCCAAGCGGGTAGGCATA
                  ************************************************************

HsCD00512429      TTCCTAGACTATGAGGCCGGCACACTGTCTTTCTACAATGTCACAGACCGCTCTCATATC
NM_021253.3       TTCCTAGACTATGAGGCCGGCACACTGTCTTTCTACAATGTCACAGACCGCTCTCATATC
NM_172016.2       TTCCTAGACTATGAGGCCGGCACACTGTCTTTCTACAATGTCACAGACCGCTCTCATATC
                  ************************************************************

HsCD00512429      TACACCTTCACTGATACTTTTACTGAGAAACTTTGGCCCCTCTTCTACCCAGGCATCCGG
NM_021253.3       TACACCTTCACTGATACTTTTACTGAGAAACTTTGGCCCCTCTTCTACCCAGGCATCCGG
NM_172016.2       TACACCTTCACTGATACTTTTACTGAGAAACTTTGGCCCCTCTTCTACCCAGGCATCCGG
                  ************************************************************

HsCD00512429      GCTGGACGGAAGAATGCTGCACCACTTACCATCAGGCCCCCAACAGATTGGGAG---
NM_021253.3       GCTGGACGGAAGAATGCTGCACCACTTACCATCAGGCCCCCAACAGATTGGGAGTGA
NM_172016.2       GCTGGACGGAAGAATGCTGCACCACTTACCATCAGGCCCCCAACAGATTGGGAGTGA
                  ******************************************************