DNASU Plasmid | KLHDC7B (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: KLHDC7B

Gene Name: kelch domain containing 7B

Original Clone ID:

ccsbBroadEn_13026

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC009980.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512521

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1486nts

Open reading frame: 1 to 1486

Reference Sequence Alignment

ATGCCCAGGGGCCCCGCACAGCCCCCCGCGCAGAGGCCGCCTGGCCCCGCGGCCTCCTCC
TCTGCGAGGCGCTCACAGCCGGTACCCCAGCTACGGAAACGCAGCAGGTGCGAAATCGCC
CCGAGCTCGGAGCAGGAGGTCAGGCCGGCCGCCTCGGGGGACCCTCAAGGGGAGGCGCCG
GGGGAGGGGGGCAGCCCTGCCGGCCGCAGCGGGGCGCTCACGGAAAAGCAGGAGGAGGCC
CGGAAGCTCATGGTGTTTCTGCAGAGGCCCGGGGGTTGGGGGGTGGTGGAGGGGCCCCGG
AAGCCCAGCTCCCGGGCCCTGGAGCCCGCCACGGCGGCAGCCCTGCGGCGGCGGCTGGAC
CTGGGCAGTTGCCTGGACGTGCTGGCCTTTGCCCAGCAGCACGGAGAGCCCGGCCTGGCG
CAGGAGACCTACGCGCTGATGAGCGACAACCTGCTGCGAGTGCTGGGAGACCCGTGCCTC
TACCGCCGGCTGAGCGCGGCCGACCGCGAGCGCATCCTCAGCCTGCGGACCGGCCGGGGC
CGGGCGGTGCTGGGCGTCCTCGTACTGCCCAGCCTCTACCAGGGGGGCCGCTCAGGGCTC
CCCAGGGGCCCTCGTGGCGAGGAGCCTCCTGCGGCGGCCCCTGTGTCCCTGCCTCTACCT
GCGCACCTGCATGTGTTCAACCCCCGGGAGAACACCTGGCGGCCCCTGACCCAGGTGCCC
GAGGAGGCCCCGCTTCGGGGCTGCGGTCTCTGCACCATGCACAACTACCTGTTTCTGGCG
GGGGGCATCCGTGGCTCCGGTGCCAAGGCCGTCTGCTCCAACGAGGTCTTCTGCTACAAC
CCTCTGACCAACATCTGGAGCCAGGTTCGGCCCATGCAGCAGGCCCGAGCCCAGCTCAAG
CTGGTGGCCCTGGACGGGCTGCTCTATGCCATCGGTGGCGAATGCCTGTACAGCATGGAG
TGCTACGACCCGCGAACAGACGCCTGGACCCCACGCGCGCCACTCCCCGCAGGCACCTTC
CCTGTGGCCCACGAGGCTGTGGCCTGCCGTGGGGACATCTACGTCACCGGGGGTCACCTC
TTCTACCGCCTGCTCAGGTACAGCCCCGTGAAGGATGCTTGGGACGAGTGCCCATACAGT
GCCAGCCACCGGCGTTCCAGCGACATCGTGGCACTGGGGGGCTTCCTGTACCGCTTCGAC
CTGCTGCGGGGCGTGGGCGCCGCCGTGATGCGCTACAACACAGTGACCGGCTCCTGGAGC
AGGGCTGCCTCCCTGCCCCTGCCCGCCCCCGCCCCACTGCACTGCACCACCCTGGGCAAC
ACCATTTACTGCCTCAACCCCCAGGTCACTGCCACCTTCACGGTCTCTGGGGGGACTGCC
CAGTTCCAGGCCAAGGAGCTGCAGCCCTTCCCCTTGGGGAGCACCGGGGTCCTCAGTCCA
TTCATCCTGACTCTGCCCCCTGAGGACCGGCTGCAGACCTCACTC

Protein sequence predicted based on nucleotide sequence

MPRGPAQPPAQRPPGPAASSSARRSQPVPQLRKRSRCEIAPSSEQEVRPAASGDPQGEAP
GEGGSPAGRSGALTEKQEEARKLMVFLQRPGGWGVVEGPRKPSSRALEPATAAALRRRLD
LGSCLDVLAFAQQHGEPGLAQETYALMSDNLLRVLGDPCLYRRLSAADRERILSLRTGRG
RAVLGVLVLPSLYQGGRSGLPRGPRGEEPPAAAPVSLPLPAHLHVFNPRENTWRPLTQVP
EEAPLRGCGLCTMHNYLFLAGGIRGSGAKAVCSNEVFCYNPLTNIWSQVRPMQQARAQLK
LVALDGLLYAIGGECLYSMECYDPRTDAWTPRAPLPAGTFPVAHEAVACRGDIYVTGGHL
FYRLLRYSPVKDAWDECPYSASHRRSSDIVALGGFLYRFDLLRGVGAAVMRYNTVTGSWS
RAASLPLPAPAPLHCTTLGNTIYCLNPQVTATFTVSGGTAQFQAKELQPFPLGSTGVLSP
FILTLPPEDRLQTSL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

KLHDC7B

Symbol Nomenclature :

KLHDC7B

Designation :

kelch domain-containing protein 7B

Full Nomenclature :

kelch domain containing 7B

GENEID :

113730

HGNC :

25145

Vega :

OTTHUMG00000150250

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00512521      ------------------------------------------------------------
NM_138433.3       ATGGTTCTTAGAAGCCACCCCTTCCCCAGGCAAGACAGGCCCCAAGGGAGTGTCCCGAGG
                                                                              

HsCD00512521      ------------------------------------------------------------
NM_138433.3       GCGGTTCCCGGGAGCCCCGTGGGTCCCAGCACTTCCACACACTCTGAGGACAGACACGGC
                                                                              

HsCD00512521      ------------------------------------------------------------
NM_138433.3       CCCTCTTCTTCAGTGGGGACAGTCATAGGGACAGGTACAGGGGGCCTGGTTGAGGCTGGA
                                                                              

HsCD00512521      ------------------------------------------------------------
NM_138433.3       GGTCAGCCACAGCCAAGAAGCTCCGAGACCAACGGATCGCCCAGCCCAGACCCTCCCCCA
                                                                              

HsCD00512521      ---------------------------------------------------------ATG
NM_138433.3       GGCCTAAGAGGAGAGGGAACCAGGGAGAAAAGTCTAGACCCGCTGCCCCAAGCCGCGATG
                                                                           ***

HsCD00512521      CCCAGGGGCCCCGCACAGCCCCCCGCGCAGAGGCCGCCTGGCCCCGCGGCCTCCTCCTCT
NM_138433.3       CCCAGGGGCCCCGCACAGCCCCCCGCGCAGAGGCCGCCTGGCCCCGCGGCCTCCTCCTCT
                  ************************************************************

HsCD00512521      GCGAGGCGCTCACAGCCGGTACCCCAGCTACGGAAACGCAGCAGGTGCGAAATCGCCCCG
NM_138433.3       GCGAGGCGCTCACAGCCGGTACCCCAGCTACGGAAACGCAGCAGGTGCGAAATCGCCCCG
                  ************************************************************

HsCD00512521      AGCTCGGAGCAGGAGGTCAGGCCGGCCGCCTCGGGGGACCCTCAAGGGGAGGCGCCGGGG
NM_138433.3       AGCTCGGAGCAGGAGGTCAGGCCGGCCGCCTCGGGGGACCCTCAAGGGGAGGCGCCGGGG
                  ************************************************************

HsCD00512521      GAGGGGGGCAGCCCTGCCGGCCGCAGCGGGGCGCTCACGGAAAAGCAGGAGGAGGCCCGG
NM_138433.3       GAGGGGGGCAGCCCTGCCGGCCGCAGCGGGGCGCTCACGGAAAAGCAGGAGGAGGCCCGG
                  ************************************************************

HsCD00512521      AAGCTCATGGTGTTTCTGCAGAGGCCCGGGGGTTGGGGGGTGGTGGAGGGGCCCCGGAAG
NM_138433.3       AAGCTCATGGTGTTTCTGCAGAGGCCCGGGGGTTGGGGGGTGGTGGAGGGGCCCCGGAAG
                  ************************************************************

HsCD00512521      CCCAGCTCCCGGGCCCTGGAGCCCGCCACGGCGGCAGCCCTGCGGCGGCGGCTGGACCTG
NM_138433.3       CCCAGCTCCCGGGCCCTGGAGCCCGCCACGGCGGCAGCCCTGCGGCGGCGGCTGGACCTG
                  ************************************************************

HsCD00512521      GGCAGTTGCCTGGACGTGCTGGCCTTTGCCCAGCAGCACGGAGAGCCCGGCCTGGCGCAG
NM_138433.3       GGCAGTTGCCTGGACGTGCTGGCCTTTGCCCAGCAGCACGGAGAGCCCGGCCTGGCGCAG
                  ************************************************************

HsCD00512521      GAGACCTACGCGCTGATGAGCGACAACCTGCTGCGAGTGCTGGGAGACCCGTGCCTCTAC
NM_138433.3       GAGACCTACGCGCTGATGAGCGACAACCTGCTGCGAGTGCTGGGAGACCCGTGCCTCTAC
                  ************************************************************

HsCD00512521      CGCCGGCTGAGCGCGGCCGACCGCGAGCGCATCCTCAGCCTGCGGACCGGCCGGGGCCGG
NM_138433.3       CGCCGGCTGAGCGCGGCCGACCGCGAGCGCATCCTCAGCCTGCGGACCGGCCGGGGCCGG
                  ************************************************************

HsCD00512521      GCGGTGCTGGGCGTCCTCGTACTGCCCAGCCTCTACCAGGGGGGCCGCTCAGGGCTCCCC
NM_138433.3       GCGGTGCTGGGCGTCCTCGTACTGCCCAGCCTCTACCAGGGGGGCCGCTCAGGGCTCCCC
                  ************************************************************

HsCD00512521      AGGGGCCCTCGTGGCGAGGAGCCTCCTGCGGCGGCCCCTGTGTCCCTGCCTCTACCTGCG
NM_138433.3       AGGGGCCCTCGTGGCGAGGAGCCTCCTGCGGCGGCCCCTGTGTCCCTGCCTCTACCTGCG
                  ************************************************************

HsCD00512521      CACCTGCATGTGTTCAACCCCCGGGAGAACACCTGGCGGCCCCTGACCCAGGTGCCCGAG
NM_138433.3       CACCTGCATGTGTTCAACCCCCGGGAGAACACCTGGCGGCCCCTGACCCAGGTGCCCGAG
                  ************************************************************

HsCD00512521      GAGGCCCCGCTTCGGGGCTGCGGTCTCTGCACCATGCACAACTACCTGTTTCTGGCGGGG
NM_138433.3       GAGGCCCCGCTTCGGGGCTGCGGTCTCTGCACCATGCACAACTACCTGTTTCTGGCGGGG
                  ************************************************************

HsCD00512521      GGCATCCGTGGCTCCGGTGCCAAGGCCGTCTGCTCCAACGAGGTCTTCTGCTACAACCCT
NM_138433.3       GGCATCCGTGGCTCCGGTGCCAAGGCCGTCTGCTCCAACGAGGTCTTCTGCTACAACCCT
                  ************************************************************

HsCD00512521      CTGACCAACATCTGGAGCCAGGTTCGGCCCATGCAGCAGGCCCGAGCCCAGCTCAAGCTG
NM_138433.3       CTGACCAACATCTGGAGCCAGGTTCGGCCCATGCAGCAGGCCCGAGCCCAGCTCAAGCTG
                  ************************************************************

HsCD00512521      GTGGCCCTGGACGGGCTGCTCTATGCCATCGGTGGCGAATGCCTGTACAGCATGGAGTGC
NM_138433.3       GTGGCCCTGGACGGGCTGCTCTATGCCATCGGTGGCGAATGCCTGTACAGCATGGAGTGC
                  ************************************************************

HsCD00512521      TACGACCCGCGAACAGACGCCTGGACCCCACGCGCGCCACTCCCCGCAGGCACCTTCCCT
NM_138433.3       TACGACCCGCGAACAGACGCCTGGACCCCACGCGCGCCACTCCCCGCAGGCACCTTCCCT
                  ************************************************************

HsCD00512521      GTGGCCCACGAGGCTGTGGCCTGCCGTGGGGACATCTACGTCACCGGGGGTCACCTCTTC
NM_138433.3       GTGGCCCACGAGGCTGTGGCCTGCCGTGGGGACATCTACGTCACCGGGGGTCACCTCTTC
                  ************************************************************

HsCD00512521      TACCGCCTGCTCAGGTACAGCCCCGTGAAGGATGCTTGGGACGAGTGCCCATACAGTGCC
NM_138433.3       TACCGCCTGCTCAGGTACAGCCCCGTGAAGGATGCTTGGGACGAGTGCCCATACAGTGCC
                  ************************************************************

HsCD00512521      AGCCACCGGCGTTCCAGCGACATCGTGGCACTGGGGGGCTTCCTGTACCGCTTCGACCTG
NM_138433.3       AGCCACCGGCGTTCCAGCGACATCGTGGCACTGGGGGGCTTCCTGTACCGCTTCGACCTG
                  ************************************************************

HsCD00512521      CTGCGGGGCGTGGGCGCCGCCGTGATGCGCTACAACACAGTGACCGGCTCCTGGAGCAGG
NM_138433.3       CTGCGGGGCGTGGGCGCCGCCGTGATGCGCTACAACACAGTGACCGGCTCCTGGAGCAGG
                  ************************************************************

HsCD00512521      GCTGCCTCCCTGCCCCTGCCCGCCCCCGCCCCACTGCACTGCACCACCCTGGGCAACACC
NM_138433.3       GCTGCCTCCCTGCCCCTGCCCGCCCCCGCCCCACTGCACTGCACCACCCTGGGCAACACC
                  ************************************************************

HsCD00512521      ATTTACTGCCTCAACCCCCAGGTCACTGCCACCTTCACGGTCTCTGGGGGGACTGCCCAG
NM_138433.3       ATTTACTGCCTCAACCCCCAGGTCACTGCCACCTTCACGGTCTCTGGGGGGACTGCCCAG
                  ************************************************************

HsCD00512521      TTCCAGGCCAAGGAGCTGCAGCCCTTCCCCTTGGGGAGCACCGGGGTCCTCAGTCCATTC
NM_138433.3       TTCCAGGCCAAGGAGCTGCAGCCCTTCCCCTTGGGGAGCACCGGGGTCCTCAGTCCATTC
                  ************************************************************

HsCD00512521      ATCCTGACTCTGCCCCCTGAGGACCGGCTGCAGACCTCACTC---
NM_138433.3       ATCCTGACTCTGCCCCCTGAGGACCGGCTGCAGACCTCACTCTGA
                  ******************************************