Gene:
Gene Symbol:
RASGRF1
Gene Name:
Ras protein-specific guanine nucleotide-releasing factor 1
Original Clone ID:
ccsbBroadEn_11089
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC040275.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00512550
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCAGAAGGGGATCCGGCTGAATGATGGCCACGTCGCGTCCCTGGGACTGCTGGCGCGC
AAGGACGGCACGCGCAAAGGCTACCTGAGCAAGCGGAGTTCGGACAACACAAAATGGCAA
ACCAAGTGGTTCGCGCTGCTGCAGAACCTGCTCTTCTACTTCGAGAGCGACTCGAGCTCG
CGGCCCTCGGGGCTTTACCTGCTGGAGGGCTGCGTCTGCGACCGCGCGCCCTCCCCCAAG
CCGGCGCTGTCGGCCAAGGAGCCGCTGGAGAAACAGCATTACTTCACGGTGAACTTCAGC
CATGAGAACCAGAAAGCCTTGGAGCTGAGGACAGAGGACGCAAAAGATTGTGACGAATGG
GTGGCAGCCATTGCACATGCCAGCTACAGGACCCTCGCCACAGAGCATGAGGCATTAATG
CAGAAATACCTGCACCTGCTGCAGATCGTGGAGACAGAGAAGACCGTGGCCAAGCAGCTT
CGGCAGCAGATCGAGGATGGGGAGATCGAGATCGAGCGGCTGAAGGCAGAGATCACATCC
CTGCTCAAGGACAATGAGCGCATCCAGTCCACCCAGACTGTCGCCCCCAACGATGAAGAC
AGCGACATCAAGAAAATTAAGAAGGTGCAGAGCTTCCTGCGGGGCTGGCTGTGCCGGCGG
AAGTGGAAGACCATCATCCAGGACTACATCCGGTCACCCCATGCTGACAGCATGCGCAAG
AGGAACCAGGTGGTGTTCAGCATGCTGGAGGCTGAGGCTGAGTACGTGCAGCAGCTGCAC
ATCCTTGTCAACAATTTCCTGCGCCCGCTGCGGATGGCCGCCAGCTCCAAGAAGCCTCCC
ATCACACACGACGACGTCAGCAGCATCTTCCTGAACAGCGAAACCATCATGTTTTTACAT
CAGATCTTTTACCAAGGCCTGAAGGCCCGCATCTCCAGCTGGCCCACGCTGGTCCTGGCT
GACCTATTTGACATCCTGCTGCCCATGCTCAACATCTACCAAGAGTTCGTCCGCAACCAC
CAGTACAGCCTGCAGATCCTGGCCCACTGCAAGCAGAACCGTGACTTCGACAAGCTGCTG
AAGCACTACGAGGCCAAGCCTGACTGCGAGGAGAGGACGCTGGAGACCTTCCTCACCTAC
CCCATGTTCCAGATCCCCAGGTACATCCTGACCCTCCATGAGCTCCTGGCCCACACGCCT
CATGAGCACGTTGAGCGCAACAGCCTGGACTACGCCAAGTCCAAACTGGAGGAGCTGTCC
AGAATAATGCACGATGAAGTAAGTGAGACGGAGAACATCCGGAAAAACCTGGCCATCGAG
CGCATGATCATCGAAGGCTGTGAGATCCTCCTGGACACCAGCCAGACCTTTGTGAGACAA
GGTTCCCTCATTCAGGTGCCCATGTCTGAAAAGGGCAAGATCACCAGGGGGCGCCTGGGG
TCTCTCTCCCTAAAGAAAGAGGGCGAGCGACAGTGCTTCCTGTTTTCTAAGCATCTGATT
ATCTGTACCAGAGGCTCTGGAGGGAAGCTTCACTTGACCAAGAATGGAGTCATATCCCTC
ATTGACTGCACTTTATTGGAGGAGCCAGAAAGCACGGAGGAGGAAGCCAAAGGATCCGGC
CAAGACATAGATCACTTGGATTTTAAAATCGGGGTGGAGCCAAAGGATTCCCCGCCCTTT
ACAGTCATCCTAGTGGCCTCGTCCAGACAGGAGAAGGCAGCGTGGACCAGTGACATCAGC
CAGTGTGTGGATAACATCCGATGCAATGGGCTCATGATGAACGCATTTGAAGAAAATTCC
AAGGTCACTGTGCCGCAGATGATCAAGTCCGACGCCTCCTTATATTGTGATGATGTTGAC
ATTCGCTTCAGCAAAACCATGAACTCCTGCAAAGTGCTGCAGATCCGCTACGCCAGTGTG
GAGCGGCTGCTGGAGAGGCTGACGGACCTGCGCTTCCTGAGCATCGACTTCCTCAACACC
TTCCTGCACTCCTACCGCGTCTTCACCACCGCCATCGTGGTCCTGGACAAGCTCATTACC
ATCTACAAGAAGCCTATCAGTGCCATTCCTGCCAGGTCGCTGGAGCTCCTGTTTGCCAGT
GGCCAGAACAATAAGCTCCTGTACGGTGAACCCCCCAAGTCCCCGCGCGCCACCCGCAAG
TTCTCCTCGCCGCCACCTCTGTCCATCACCAAGACATCGTCACCGAGCCGCCGGCGGAAG
CTCTCCCTGAACATCCCCATCATCACTGGCGGCAAGGCCCTGGACCTGGCCGCCCTCAGC
TGCAACTCCAATGGCTACACCAGCATGTACTCGGCCATGTCACCCTTCAGCAAGGCCACG
CTGGACACCAGCAAGCTCTATGTGTCCAGCAGCTTCACCAACAAGATTCCAGATGAGGGC
GATACGACCCCTGAGAAGCCCGAAGACCCTTCAGCGCTCAGCAAGCAGAGCTCAGAAGTC
TCCATGAGAGAGGAGTCAGATATTGATCAAAACCAGAGTGATGATGGTGATACTGAAACA
TCACCAACTAAATCTCCAACAACACCCAAATCAGTCAAAAACAAAAATTCTTCAGAGTTC
CCACTCTTTTCCTATAACAATGGAGTCGTCATGACCTCCTGTCGTGAACTGGACAATAAC
CGCAGTGCCTTGTCGGCCGCCTCTGCCTTTGCCATAGCAACCGCCGGGGCCAACGAGGGC
ACCCCAAACAAGGAGAAGTACCGGAGGATGTCCTTAGCCAGTGCAGGGTTTCCCCCAGAC
CAGAGGAATGGAGACAAGGAGTTTGTGATCCGCAGAGCAGCCACCAATCGTGTCTTGAAC
GTGCTCCGCCACTGGGTGTCCAAGCACTCTCAGGACTTTGAGACCAACGATGAGCTCAAA
TGCAAGGTGATCGGCTTCCTGGAAGAAGTCATGCACGACCCGGAGCTCCTGACCCAGGAG
CGGAAGGCTGCAGCCAACATCATCAGGACTCTGACCCAGGAGGACCCAGGTGACAACCAG
ATCACGCTGGAGGAGATCACGCAGATGGCTGAAGGCGTGAAGGCTGAGCCCTTTGAAAAC
CACTCAGCCCTGGAGATCGCGGAGCAGCTGACCCTGCTAGATCACCTCGTCTTCAAGAAG
ATTCCTTATGAGGAGTTCTTCGGACAAGGATGGATGAAACTGGAAAAGAATGAAAGGACC
CCTTATATCATGAAAACCACTAAGCACTTCAATGACATCAGTAACTTGATTGCTTCAGAA
ATCATCCGCAACGAGGACATCAACGCCAGGGTGAGCGCCATCGAGAAGTGGGTGGCCGTA
GCTGACATATGCCGCTGCCTCCACAACTACAATGCCGTACTGGAGATCACCTCGTCCATG
AACCGCAGTGCAATCTTCCGGCTCAAAAAGACGTGGCTCAAAGTCTCTAAGCAGGTGCGG
GCAGGAGGCTGGCACCACTGCCTCCCTGGGGCTGGATTGGGGCTGGGGCAGGAAGACCCG
TGGGCTGGCCACTGGGCATCCACAAGTGGGCAAAGGACTGGGCTGAGGTCTAACACTCCC
AGG
Protein sequence predicted based on nucleotide sequence
MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSS RPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEW VAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEITS LLKDNERIQSTQTVAPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRK RNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLH QIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLL KHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELS RIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLG SLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSG QDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENS KVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNT FLHSYRVFTTAIVVLDKLITIYKKPISAIPARSLELLFASGQNNKLLYGEPPKSPRATRK FSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKAT LDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSALSKQSSEVSMREESDIDQNQSDDGDTET SPTKSPTTPKSVKNKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEG TPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELK CKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFEN HSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASE IIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQVR AGGWHHCLPGAGLGLGQEDPWAGHWASTSGQRTGLRSNTPR
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
