Gene:
Gene Symbol:
LOC81691
Gene Name:
exonuclease NEF-sp
Original Clone ID:
ccsbBroadEn_09081
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC007646.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00512566
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGCCAGAGAGGGAAGGGACCGAGAGACACCCCAGGAAGGTCAGGGAAAGCAGGCAG
GCCCCAAATAAGCTGGTCGGGGCAGCTGAGGCGATGAAAGCCGGTTGGGATCTCGAGGAG
AGTCAGCCCGAGGCCAAGAAAGCCCGCTTATCTACCATTTTATTTACTGACAACTGTGAA
GTAACCCATGACCAGCTGTGTGAATTGCTGAAGTATGCAGTTCTGGGCAAATCCAATGTT
CCAAAACCCAGCTGGTGCCAGCTTTTTCATCAAAACCACCTAAACAACGTAGTGGTTTTT
GTTCTGCAGGGAATGAGTCAGCTACACTTTTACAGGTTCTATTTGGAGTTTGGATGTCTT
CGAAAAGCATTCAGACATAAATTCCGCTTGCCTCCACCATCATCTGATTTTCTAGCTGAT
GTTGTTGGGCTACAAACTGAACAAAGAGCTGGAGATCTGCCCAAGACAATGGAAGGGCCT
TTACCTTCTAATGCAAAAGCCGCCATCAACCTTCAGGATGATCCCATCATTCAAAAGTAT
GGCTCTAAGAAAGTGGGCTTGACCAGATGCCTTCTGACAAAGGAGGAAATGAGAACGTTT
CACTTTCCATTACAAGGTTTTCCTGATTGTGAAAACTTTTTACTTACCAAATGTAATGGT
TCTATAGCAGACAATAGTCCTCTCTTTGGACTTGACTGTGAAATGTGCCTCACATCCAAG
GGGAGAGAGCTAACACGCATCTCACTGGTTGCTGAAGGAGGCTGCTGTGTTATGGATGAA
CTGGTCAAACCTGAAAACAAGATTCTGGACTACCTCACCAGCTTTTCGGGAATCACGAAG
AAGATTCTTAACCCAGTGACGACCAAACTCAAAGATGTACAGAGGCAGTTAAAAGCACTG
CTTCCTCCTGATGCTGTGTTAGTGGGCCACTCCTTAGATTTGGATCTCAGAGCACTGAAA
ATGATACATCCATATGTTATTGATACATCGTTGCTTTATGTCAGAGAGCAGGGCAGAAGA
TTTAAGCTCAAGTTCTTAGCCAAAGTTATTTTGGGGAAGGATATACAGTGTCCAGACAGA
CTTGGTCATGATGCCACAGAAGATGCTAGAACAATCCTTGAATTGGCTCGGTATTTCCTT
AAGCATGGCCCAAAAAAGATTGCAGAACTAAATCTAGAAGCACTAGCTAATCACCAAGAA
ATACAAGCAGCAGGCCAAGAGCCTAAAAACACAGCAGAAGTACTTCAGCACCCAAACACA
AGTGTTTTAGAATGCTTGGATTCAGTGGGTCAGAAGCTTCTTTTTTTGACCCGGGAGACA
GATGCTGGTGAACTTCCATCTTCCAGAAATTGTCAAACTATTAAGTGTCTTTCAAATAAA
GAGGTTCTTGAGCAGGCCAGAGTGGAAATCCCCCTGTTTCCCTTCAGCATTGTTCAGTTC
TCTTTTAAGGCCTTTTCACCTGTCCTCACTGAGGAGATGAACAAAAGGATGAGGATCAAG
TGGACAGAGATATCAACTGTCTATGCTGGGCCATTTAGCAAAAATTGCAATCTCAGGGCT
CTGAAGAGGCTGTTTAAAAGCTTTGGCCCAGTCCAGTCAATGACTTTTGTTCTTGAAACC
CGTCAGCCTCATCTCTGTATACAGTATGAAGTCCTAGAAGCTGCCCAGCTGGCCATAGAG
TCCTTGGATGGTATTCTGGTAGATGGTATCTGCATCAAGGTGCAGAGGCCTGTGACAGAG
CTCACGCTTGATTGTGACACCCTCGTGAATGAGCTGGAAGGAGATTCTGAAAACCAAGGC
TCTATATATCTGTCTGGAGTGAGTGAAACCTTCAAAGAACAGCTATTGCAGGAGCCCCGC
CTCTTTCTTGGCCTGGAAGCTGTGATCTTGCCTAAAGATCTTAAAAGTGGAAAGCAGAAA
AAATACTGTTTCCTGAAATTCAAAAGTTTTGGCAGTGCCCAGCAGGCCCTCAACATTCTC
ACAGGCAAGGACTGGAAGCTGAAAGGCAGGCATGCCCTAACCCCCAGGCACCTCCATGCC
TGGCTCAGAGGCTTACCACCTGAATCAACAAGGCTCCCAGGGCTTCGTGTTGTACCTCCC
CCCTTTGAACAGGAGGCCTTGCAGACTCTGAAACTGGACCACCCGAAGATAGCAGCCTGG
CGCTGGAGCCGGAAGATTGGAAAGCTCTACAACAGCTTGTGCCCGGGCACTCTCTGCCTC
ATCCTGCTGCCAGGAACCAAGAGCACTCATGGTTCACTCTCTGGTCTAGGACTGATGGGA
ATAAAAGAGGAAGAAGAAAGCGCTGGCCCAGGCCTGTGTTCG
Protein sequence predicted based on nucleotide sequence
MEPEREGTERHPRKVRESRQAPNKLVGAAEAMKAGWDLEESQPEAKKARLSTILFTDNCE VTHDQLCELLKYAVLGKSNVPKPSWCQLFHQNHLNNVVVFVLQGMSQLHFYRFYLEFGCL RKAFRHKFRLPPPSSDFLADVVGLQTEQRAGDLPKTMEGPLPSNAKAAINLQDDPIIQKY GSKKVGLTRCLLTKEEMRTFHFPLQGFPDCENFLLTKCNGSIADNSPLFGLDCEMCLTSK GRELTRISLVAEGGCCVMDELVKPENKILDYLTSFSGITKKILNPVTTKLKDVQRQLKAL LPPDAVLVGHSLDLDLRALKMIHPYVIDTSLLYVREQGRRFKLKFLAKVILGKDIQCPDR LGHDATEDARTILELARYFLKHGPKKIAELNLEALANHQEIQAAGQEPKNTAEVLQHPNT SVLECLDSVGQKLLFLTRETDAGELPSSRNCQTIKCLSNKEVLEQARVEIPLFPFSIVQF SFKAFSPVLTEEMNKRMRIKWTEISTVYAGPFSKNCNLRALKRLFKSFGPVQSMTFVLET RQPHLCIQYEVLEAAQLAIESLDGILVDGICIKVQRPVTELTLDCDTLVNELEGDSENQG SIYLSGVSETFKEQLLQEPRLFLGLEAVILPKDLKSGKQKKYCFLKFKSFGSAQQALNIL TGKDWKLKGRHALTPRHLHAWLRGLPPESTRLPGLRVVPPPFEQEALQTLKLDHPKIAAW RWSRKIGKLYNSLCPGTLCLILLPGTKSTHGSLSGLGLMGIKEEEESAGPGLCS
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
