DNASU Plasmid | CRISPLD2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: CRISPLD2

Gene Name: cysteine-rich secretory protein LCCL domain containing 2

Original Clone ID:

ccsbBroadEn_12752

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC063012.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512840

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1348nts

Open reading frame: 1 to 1348

Reference Sequence Alignment

ATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCC
CAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCAC
AACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTC
ATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATG
ACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAG
CACGGGCCCACCGGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGG
TATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTAC
CCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCTATGTGCACG
CACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGC
CGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTAT
TCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAG
TGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTAC
ACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAA
AACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCG
GTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGG
TCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTT
GGAAGTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATC
CTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTG
AAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATG
GTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCG
TTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAA
CCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATGTGAGTAGGATGCAT
TTTCAACAACTATCTCGCAAAGCCTGT

Protein sequence predicted based on nucleotide sequence

MSCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEIL
MLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTGLLVSIGQNLGAHWGR
YRSPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTC
RKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETY
TPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKG
STCNRYQCPAGCLNHKAKIFGSLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFV
KSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDE
PSYWAPVFGTNIYADVSRMHFQQLSRKAC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

CRISPLD2

Symbol Nomenclature :

CRISPLD2

SYNONYM :

CRISP11; LCRISP2

Designation :

CRISP-11|LCCL domain containing cysteine-rich secretory protein 2|LCCL domain-containing cysteine-rich secretory protein 2|cysteine-rich secretory protein 11|cysteine-rich secretory protein LCCL domain-containing 2

Full Nomenclature :

cysteine-rich secretory protein LCCL domain containing 2

GENEID :

83716

Locus Tag :

UNQ2914/PRO1156/PRO9783

GenBank Accession :

AM393137

HGNC :

25248

MIM :

612434

Vega :

OTTHUMG00000137644

Target GenBank:

AM393137

Reference Sequence Alignment

  

HsCD00512840        ATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCC
XM_005256190.1      ATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCC
NM_031476.3         ATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCC
                    ************************************************************

HsCD00512840        CAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCAC
XM_005256190.1      CAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCAC
NM_031476.3         CAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCAC
                    ************************************************************

HsCD00512840        AACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTC
XM_005256190.1      AACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTC
NM_031476.3         AACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTC
                    ************************************************************

HsCD00512840        ATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATG
XM_005256190.1      ATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATG
NM_031476.3         ATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATG
                    ************************************************************

HsCD00512840        ACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAG
XM_005256190.1      ACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAG
NM_031476.3         ACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAG
                    ************************************************************

HsCD00512840        CACGGGCCCACCGGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGG
XM_005256190.1      CACGGGCCCACCAGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGG
NM_031476.3         CACGGGCCCACCAGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGG
                    ************.***********************************************

HsCD00512840        TATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTAC
XM_005256190.1      TATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTAC
NM_031476.3         TATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTAC
                    ************************************************************

HsCD00512840        CCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCTATGTGCACG
XM_005256190.1      CCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCCATGTGCACG
NM_031476.3         CCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCCATGTGCACG
                    ************************************************** *********

HsCD00512840        CACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGC
XM_005256190.1      CACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGC
NM_031476.3         CACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGC
                    ************************************************************

HsCD00512840        CGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTAT
XM_005256190.1      CGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTAT
NM_031476.3         CGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTAT
                    ************************************************************

HsCD00512840        TCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAG
XM_005256190.1      TCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAG
NM_031476.3         TCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAG
                    ************************************************************

HsCD00512840        TGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTAC
XM_005256190.1      TGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTAC
NM_031476.3         TGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTAC
                    ************************************************************

HsCD00512840        ACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAA
XM_005256190.1      ACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAA
NM_031476.3         ACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAA
                    ************************************************************

HsCD00512840        AACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCG
XM_005256190.1      AACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCG
NM_031476.3         AACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCG
                    ************************************************************

HsCD00512840        GTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGG
XM_005256190.1      GTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGG
NM_031476.3         GTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGG
                    ************************************************************

HsCD00512840        TCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTT
XM_005256190.1      TCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTT
NM_031476.3         TCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTT
                    ************************************************************

HsCD00512840        GGAAGTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATC
XM_005256190.1      GGAACTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATC
NM_031476.3         GGAACTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATC
                    **** *******************************************************

HsCD00512840        CTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTG
XM_005256190.1      CTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTG
NM_031476.3         CTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTG
                    ************************************************************

HsCD00512840        AAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATG
XM_005256190.1      AAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATG
NM_031476.3         AAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATG
                    ************************************************************

HsCD00512840        GTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCG
XM_005256190.1      GTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCG
NM_031476.3         GTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCG
                    ************************************************************

HsCD00512840        TTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAA
XM_005256190.1      TTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAA
NM_031476.3         TTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAA
                    ************************************************************

HsCD00512840        CCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATGTGAGTAGGATGCAT
XM_005256190.1      CCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGC
NM_031476.3         CCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGC
                    *********************************************.  : :** **  . 

HsCD00512840        TTTCAACAACTATCTCGCAAAGCCTGT---------------------------------
XM_005256190.1      AAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATG
NM_031476.3         AAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATG
                    :: ..* .. *. .    ..** *:                                   

HsCD00512840        ------------------------------------------------------------
XM_005256190.1      CCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGC
NM_031476.3         CCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGC
                                                                                

HsCD00512840        ------------------------------------------------------
XM_005256190.1      CTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGA
NM_031476.3         CTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGA