DNASU Plasmid | FAF2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: FAF2

Gene Name: Fas associated factor family member 2

Original Clone ID:

ccsbBroadEn_02735

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC001069.1,BC006145.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512869

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1336nts

Open reading frame: 1 to 1336

Reference Sequence Alignment

ATGGCGGCGCCTGAGGAGCGGGATCTAACCCAGGAGCAGACAGAGAAGCTGCTGCAGTTT
CAGGATCTCACTGGCATCGAATCTATGGATCAGTGTCGCCATACCTTGGAACAGCATAAC
TGGAACATAGAGGCTGCTGTACAGGACAGATTGAATGAGCAAGAGGGCGTACCTAGTGTT
TTCAACCCACCTCCATCACGACCCCTGCAGGTTAATACAGCTGACCACAGGATCTACAGC
TATGTTGTCTCAAGACCTCAACCAAGGGGGCTGCTTGGATGGGGTTATTACTTGATAATG
CTTCCATTCCGGTTTACCTATTACACGATACTTGATATATTTAGGTTTGCTCTTCGTTTT
ATACGGCCTGACCCTCGCAGCCGGGTCACTGACCCCGTTGGGGACATTGTTTCATTTATG
CACTCTTTTGAAGAGAAATATGGGAGGGCACACCCTGTCTTCTACCAGGGAACGTACAGC
CAGGCACTTAACGATGCCAAAAGGGAGCTTCGCTTTCTTTTGGTTTATCTTCATGGAGAT
GATCACCAGGACTCTGATGAGTTTTGTCGCAACACACTCTGTGCACCTGAAGTTATTTCA
CTAATAAACACTAGGATGCTCTTCTGGGCATGCTCTACAAACAAACCTGAGGGATACAGG
GTCTCACAGGCTTTACGAGAGAACACCTATCCATTCCTGGCCATGATTATGCTGAAGGAT
CGAAGGATGACTGTGGTGGGACGGCTAGAAGGCCTCATTCAACCTGATGACCTCATTAAC
CAACTGACATTTATCATGGATGCTAACCAGACTTACCTGGTGTCAGAACGCCTAGAAAGG
GAAGAAAGAAACCAGACCCAAGTGCTGAGACAACAGCAGGATGAGGCCTACCTGGCCTCT
CTCAGAGCTGACCAGGAGAAAGAAAGAAAGAAACGGGAGGAGCGGGAGCGTAAGCGGCGG
AAGGAGGAGGAGGTGCAACAGCAAAAGTTGGCAGAGGAGAGACGGCGGCAGAATTTACAG
GAGGAAAAGGAAAGGAAGTTGGAATGCCTGCCCCCTGAACCTTCCCCTGATGACCCTGAA
AGTGTCAAGATCATCTTCAAATTACCTAATGATTCTCGAGTAGAGAGACGATTCCACTTT
TCACAGTCTCTAACAGTAATCCACGACTTCTTATTCTCCTTGAAGGAAAGCCCAGAAAAG
TTTCAGATTGAAGCCAATTTTCCCAGGCGAGTGCTGCCCTGCATCCCTTCAGAGGAGTGG
CCCAATCCCCCTACGCTACAGGAGGCCGGACTCAGCCACACAGAAGTTCTTTTTGTTCAG
GACCTAACTGACGAA

Protein sequence predicted based on nucleotide sequence

MAAPEERDLTQEQTEKLLQFQDLTGIESMDQCRHTLEQHNWNIEAAVQDRLNEQEGVPSV
FNPPPSRPLQVNTADHRIYSYVVSRPQPRGLLGWGYYLIMLPFRFTYYTILDIFRFALRF
IRPDPRSRVTDPVGDIVSFMHSFEEKYGRAHPVFYQGTYSQALNDAKRELRFLLVYLHGD
DHQDSDEFCRNTLCAPEVISLINTRMLFWACSTNKPEGYRVSQALRENTYPFLAMIMLKD
RRMTVVGRLEGLIQPDDLINQLTFIMDANQTYLVSERLEREERNQTQVLRQQQDEAYLAS
LRADQEKERKKREERERKRRKEEEVQQQKLAEERRRQNLQEEKERKLECLPPEPSPDDPE
SVKIIFKLPNDSRVERRFHFSQSLTVIHDFLFSLKESPEKFQIEANFPRRVLPCIPSEEW
PNPPTLQEAGLSHTEVLFVQDLTDE

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

FAF2

Symbol Nomenclature :

FAF2

SYNONYM :

ETEA; UBXD8; UBXN3B

Designation :

Full Nomenclature :

Fas associated factor family member 2

GENEID :

23197

GenBank Accession :

DQ896293

HGNC :

24666

Vega :

OTTHUMG00000163228

Target GenBank:

DQ896293

Reference Sequence Alignment

  

HsCD00512869      ATGGCGGCGCCTGAGGAGCGGGATCTAACCCAGGAGCAGACAGAGAAGCTGCTGCAGTTT
NM_014613.2       ATGGCGGCGCCTGAGGAGCGGGATCTAACCCAGGAGCAGACAGAGAAGCTGCTGCAGTTT
                  ************************************************************

HsCD00512869      CAGGATCTCACTGGCATCGAATCTATGGATCAGTGTCGCCATACCTTGGAACAGCATAAC
NM_014613.2       CAGGATCTCACTGGCATCGAATCTATGGATCAGTGTCGCCATACCTTGGAACAGCATAAC
                  ************************************************************

HsCD00512869      TGGAACATAGAGGCTGCTGTACAGGACAGATTGAATGAGCAAGAGGGCGTACCTAGTGTT
NM_014613.2       TGGAACATAGAGGCTGCTGTACAGGACAGATTGAATGAGCAAGAGGGCGTACCTAGTGTT
                  ************************************************************

HsCD00512869      TTCAACCCACCTCCATCACGACCCCTGCAGGTTAATACAGCTGACCACAGGATCTACAGC
NM_014613.2       TTCAACCCACCTCCATCACGACCCCTGCAGGTTAATACAGCTGACCACAGGATCTACAGC
                  ************************************************************

HsCD00512869      TATGTTGTCTCAAGACCTCAACCAAGGGGGCTGCTTGGATGGGGTTATTACTTGATAATG
NM_014613.2       TATGTTGTCTCAAGACCTCAACCAAGGGGGCTGCTTGGATGGGGTTATTACTTGATAATG
                  ************************************************************

HsCD00512869      CTTCCATTCCGGTTTACCTATTACACGATACTTGATATATTTAGGTTTGCTCTTCGTTTT
NM_014613.2       CTTCCATTCCGGTTTACCTATTACACGATACTTGATATATTTAGGTTTGCTCTTCGTTTT
                  ************************************************************

HsCD00512869      ATACGGCCTGACCCTCGCAGCCGGGTCACTGACCCCGTTGGGGACATTGTTTCATTTATG
NM_014613.2       ATACGGCCTGACCCTCGCAGCCGGGTCACTGACCCCGTTGGGGACATTGTTTCATTTATG
                  ************************************************************

HsCD00512869      CACTCTTTTGAAGAGAAATATGGGAGGGCACACCCTGTCTTCTACCAGGGAACGTACAGC
NM_014613.2       CACTCTTTTGAAGAGAAATATGGGAGGGCACACCCTGTCTTCTACCAGGGAACGTACAGC
                  ************************************************************

HsCD00512869      CAGGCACTTAACGATGCCAAAAGGGAGCTTCGCTTTCTTTTGGTTTATCTTCATGGAGAT
NM_014613.2       CAGGCACTTAACGATGCCAAAAGGGAGCTTCGCTTTCTTTTGGTTTATCTTCATGGAGAT
                  ************************************************************

HsCD00512869      GATCACCAGGACTCTGATGAGTTTTGTCGCAACACACTCTGTGCACCTGAAGTTATTTCA
NM_014613.2       GATCACCAGGACTCTGATGAGTTTTGTCGCAACACACTCTGTGCACCTGAAGTTATTTCA
                  ************************************************************

HsCD00512869      CTAATAAACACTAGGATGCTCTTCTGGGCATGCTCTACAAACAAACCTGAGGGATACAGG
NM_014613.2       CTAATAAACACTAGGATGCTCTTCTGGGCATGCTCTACAAACAAACCTGAGGGATACAGG
                  ************************************************************

HsCD00512869      GTCTCACAGGCTTTACGAGAGAACACCTATCCATTCCTGGCCATGATTATGCTGAAGGAT
NM_014613.2       GTCTCACAGGCTTTACGAGAGAACACCTATCCATTCCTGGCCATGATTATGCTGAAGGAT
                  ************************************************************

HsCD00512869      CGAAGGATGACTGTGGTGGGACGGCTAGAAGGCCTCATTCAACCTGATGACCTCATTAAC
NM_014613.2       CGAAGGATGACTGTGGTGGGACGGCTAGAAGGCCTCATTCAACCTGATGACCTCATTAAC
                  ************************************************************

HsCD00512869      CAACTGACATTTATCATGGATGCTAACCAGACTTACCTGGTGTCAGAACGCCTAGAAAGG
NM_014613.2       CAACTGACATTTATCATGGATGCTAACCAGACTTACCTGGTGTCAGAACGCCTAGAAAGG
                  ************************************************************

HsCD00512869      GAAGAAAGAAACCAGACCCAAGTGCTGAGACAACAGCAGGATGAGGCCTACCTGGCCTCT
NM_014613.2       GAAGAAAGAAACCAGACCCAAGTGCTGAGACAACAGCAGGATGAGGCCTACCTGGCCTCT
                  ************************************************************

HsCD00512869      CTCAGAGCTGACCAGGAGAAAGAAAGAAAGAAACGGGAGGAGCGGGAGCGTAAGCGGCGG
NM_014613.2       CTCAGAGCTGACCAGGAGAAAGAAAGAAAGAAACGGGAGGAGCGGGAGCGTAAGCGGCGG
                  ************************************************************

HsCD00512869      AAGGAGGAGGAGGTGCAACAGCAAAAGTTGGCAGAGGAGAGACGGCGGCAGAATTTACAG
NM_014613.2       AAGGAGGAGGAGGTGCAACAGCAAAAGTTGGCAGAGGAGAGACGGCGGCAGAATTTACAG
                  ************************************************************

HsCD00512869      GAGGAAAAGGAAAGGAAGTTGGAATGCCTGCCCCCTGAACCTTCCCCTGATGACCCTGAA
NM_014613.2       GAGGAAAAGGAAAGGAAGTTGGAATGCCTGCCCCCTGAACCTTCCCCTGATGACCCTGAA
                  ************************************************************

HsCD00512869      AGTGTCAAGATCATCTTCAAATTACCTAATGATTCTCGAGTAGAGAGACGATTCCACTTT
NM_014613.2       AGTGTCAAGATCATCTTCAAATTACCTAATGATTCTCGAGTAGAGAGACGATTCCACTTT
                  ************************************************************

HsCD00512869      TCACAGTCTCTAACAGTAATCCACGACTTCTTATTCTCCTTGAAGGAAAGCCCAGAAAAG
NM_014613.2       TCACAGTCTCTAACAGTAATCCACGACTTCTTATTCTCCTTGAAGGAAAGCCCAGAAAAG
                  ************************************************************

HsCD00512869      TTTCAGATTGAAGCCAATTTTCCCAGGCGAGTGCTGCCCTGCATCCCTTCAGAGGAGTGG
NM_014613.2       TTTCAGATTGAAGCCAATTTTCCCAGGCGAGTGCTGCCCTGCATCCCTTCAGAGGAGTGG
                  ************************************************************

HsCD00512869      CCCAATCCCCCTACGCTACAGGAGGCCGGACTCAGCCACACAGAAGTTCTTTTTGTTCAG
NM_014613.2       CCCAATCCCCCTACGCTACAGGAGGCCGGACTCAGCCACACAGAAGTTCTTTTTGTTCAG
                  ************************************************************

HsCD00512869      GACCTAACTGACGAA---
NM_014613.2       GACCTAACTGACGAATGA
                  ***************