DNASU Plasmid | WASL (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: WASL

Gene Name: Wiskott-Aldrich syndrome-like

Original Clone ID:

ccsbBroadEn_07334

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC052955.1

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512949

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1516nts

Open reading frame: 1 to 1516

Reference Sequence Alignment

ATGAGCTCCGTCCAGCAGCAGCCGCCGCCGCCGCGGAGGGTCACCAACGTGGGGTCCCTG
TTGCTCACCCCGCAGGAGAACGAGTCCCTCTTCACTTTCCTCGGCAAGAAATGTGTGACT
ATGTCTTCAGCAGTGGTGCAGTTATATGCAGCAGATCGGAACTGTATGTGGTCAAAGAAG
TGCAGTGGTGTTGCTTGTCTTGTTAAGGACAATCCACAGAGATCTTATTTTTTAAGAATA
TTTGACATTAAGGATGGGAAACTATTGTGGGAACAAGAGCTATACAATAACTTTGTATAT
AATAGTCCTAGAGGATATTTTCATACCTTTGCTGGAGATACTTGTCAAGTTGCTCTTAAT
TTTGCCAATGAAGAAGAAGCAAAAAAATTTCGAAAAGCAGTTACAGACCTTTTGGGCCGT
CGACAAAGGAAATCTGAGAAAAGACGAGATCCCCCAAATGGTCCTAATCTACCCATGGCT
ACAGTTGATATAAAAAATCCAGAAATCACAACAAATAGATTTTATGGTCCACAAGTCAAC
AACATCTCCCATACCAAAGAAAAGAAGAAGGGAAAAGCTAAAAAGAAGAGATTAACCAAG
GCAGATATAGGAACACCAAGCAATTTCCAGCACATTGGACATGTTGGTTGGGATCCAAAT
ACAGGCTTTGATCTGAATAATTTGGATCCAGAATTGAAGAATCTTTTCGATATGTGTGGA
ATCTCAGAGGCACAACTTAAAGACAGAGAAACATCAAAAGTTATATATGACTTTATTGAA
AAAACAGGAGGTGTTGAAGCTGTTAAAAATGAACTGCGGAGGCAAGCACCACCACCTCCA
CCACCATCAAGGGGAGGGCCACCTCCTCCTCCTCCCCCTCCACACAACTCAGGTCCTCCT
CCTCCTCCTGCTAGGGGAAGAGGCGCTCCTCCCCCACCACCTTCAAGAGCTCCCACAGCT
GCACCTCCACCACCGCCTCCTTCCAGGCCAAGTGTAGCAGTCCCTCCACCACCGCCAAAT
AGGATGTACCCTCCTCCACCTCCAGCCCTTCCCTCCTCAGCACCTTCAGGGCCTCCACCA
CCACCTCCATCTGTGTTGGGGGTAGGGCCAGTGGCACCACCCCCACCGCCTCCACCTCCC
CCTCCTCCTGGGCCACCGCCCCCGCCTGGCCTGCCTTCTGATGGGGACCATCAGGTTCCA
ACTACTGCAGGAAACAAAGCAGCTCTTTTAGATCAAATTAGAGAGGGTGCTCAGCTAAAA
AAAGTGGAGCAGAACAGTCGGCCAGTGTCCTGCTCTGGACGAGATGCACTGTTAGACCAG
ATACGACAGGGTATCCAACTAAAATCTGTGGCTGATGGCCAAGAGTCTACACCACCAACA
CCTGCACCCACTTCAGGAATTGTGGGTGCATTAATGGAAGTGATGCAGAAAAGGAGCAAA
GCCATTCATTCTTCAGATGAAGATGAAGATGAAGATGATGAAGAAGATTTTGAGGATGAT
GATGAGTGGGAAGAC

Protein sequence predicted based on nucleotide sequence

MSSVQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKK
CSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALN
FANEEEAKKFRKAVTDLLGRRQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQVN
NISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCG
ISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHNSGPP
PPPARGRGAPPPPPSRAPTAAPPPPPPSRPSVAVPPPPPNRMYPPPPPALPSSAPSGPPP
PPPSVLGVGPVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTTAGNKAALLDQIREGAQLK
KVEQNSRPVSCSGRDALLDQIRQGIQLKSVADGQESTPPTPAPTSGIVGALMEVMQKRSK
AIHSSDEDEDEDDEEDFEDDDEWED

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

1 error

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

WASL

Symbol Nomenclature :

WASL

SYNONYM :

N-WASP; NWASP

Designation :

neural Wiskott-Aldrich syndrome protein

Full Nomenclature :

Wiskott-Aldrich syndrome-like

GENEID :

8976

HGNC :

12735

MIM :

605056

Vega :

OTTHUMG00000157346

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00512949      ATGAGCTCCGTCCAGCAGCAGCCGCCGCCGCCGCGGAGGGTCACCAACGTGGGGTCCCTG
NM_003941.3       ATGAGCTCCGTCCAGCAGCAGCCGCCGCCGCCGCGGAGGGTCACCAACGTGGGGTCCCTG
                  ************************************************************

HsCD00512949      TTGCTCACCCCGCAGGAGAACGAGTCCCTCTTCACTTTCCTCGGCAAGAAATGTGTGACT
NM_003941.3       TTGCTCACCCCGCAGGAGAACGAGTCCCTCTTCACTTTCCTCGGCAAGAAATGTGTGACT
                  ************************************************************

HsCD00512949      ATGTCTTCAGCAGTGGTGCAGTTATATGCAGCAGATCGGAACTGTATGTGGTCAAAGAAG
NM_003941.3       ATGTCTTCAGCAGTGGTGCAGTTATATGCAGCAGATCGGAACTGTATGTGGTCAAAGAAG
                  ************************************************************

HsCD00512949      TGCAGTGGTGTTGCTTGTCTTGTTAAGGACAATCCACAGAGATCTTATTTTTTAAGAATA
NM_003941.3       TGCAGTGGTGTTGCTTGTCTTGTTAAGGACAATCCACAGAGATCTTATTTTTTAAGAATA
                  ************************************************************

HsCD00512949      TTTGACATTAAGGATGGGAAACTATTGTGGGAACAAGAGCTATACAATAACTTTGTATAT
NM_003941.3       TTTGACATTAAGGATGGGAAACTATTGTGGGAACAAGAGCTATACAATAACTTTGTATAT
                  ************************************************************

HsCD00512949      AATAGTCCTAGAGGATATTTTCATACCTTTGCTGGAGATACTTGTCAAGTTGCTCTTAAT
NM_003941.3       AATAGTCCTAGAGGATATTTTCATACCTTTGCTGGAGATACTTGTCAAGTTGCTCTTAAT
                  ************************************************************

HsCD00512949      TTTGCCAATGAAGAAGAAGCAAAAAAATTTCGAAAAGCAGTTACAGACCTTTTGGGCCGT
NM_003941.3       TTTGCCAATGAAGAAGAAGCAAAAAAATTTCGAAAAGCAGTTACAGACCTTTTGGGCCGT
                  ************************************************************

HsCD00512949      CGACAAAGGAAATCTGAGAAAAGACGAGATCCCCCAAATGGTCCTAATCTACCCATGGCT
NM_003941.3       CGACAAAGGAAATCTGAGAAAAGACGAGATCCCCCAAATGGTCCTAATCTACCCATGGCT
                  ************************************************************

HsCD00512949      ACAGTTGATATAAAAAATCCAGAAATCACAACAAATAGATTTTATGGTCCACAAGTCAAC
NM_003941.3       ACAGTTGATATAAAAAATCCAGAAATCACAACAAATAGATTTTATGGTCCACAAGTCAAC
                  ************************************************************

HsCD00512949      AACATCTCCCATACCAAAGAAAAGAAGAAGGGAAAAGCTAAAAAGAAGAGATTAACCAAG
NM_003941.3       AACATCTCCCATACCAAAGAAAAGAAGAAGGGAAAAGCTAAAAAGAAGAGATTAACCAAG
                  ************************************************************

HsCD00512949      GCAGATATAGGAACACCAAGCAATTTCCAGCACATTGGACATGTTGGTTGGGATCCAAAT
NM_003941.3       GCAGATATAGGAACACCAAGCAATTTCCAGCACATTGGACATGTTGGTTGGGATCCAAAT
                  ************************************************************

HsCD00512949      ACAGGCTTTGATCTGAATAATTTGGATCCAGAATTGAAGAATCTTTTCGATATGTGTGGA
NM_003941.3       ACAGGCTTTGATCTGAATAATTTGGATCCAGAATTGAAGAATCTTTTCGATATGTGTGGA
                  ************************************************************

HsCD00512949      ATCTCAGAGGCACAACTTAAAGACAGAGAAACATCAAAAGTTATATATGACTTTATTGAA
NM_003941.3       ATCTCAGAGGCACAACTTAAAGACAGAGAAACATCAAAAGTTATATATGACTTTATTGAA
                  ************************************************************

HsCD00512949      AAAACAGGAGGTGTTGAAGCTGTTAAAAATGAACTGCGGAGGCAAGCACCACCACCTCCA
NM_003941.3       AAAACAGGAGGTGTTGAAGCTGTTAAAAATGAACTGCGGAGGCAAGCACCACCACCTCCA
                  ************************************************************

HsCD00512949      CCACCATCAAGGGGAGGGCCACCTCCTCCTCCTCCCCCTCCACACAACTCAGGTCCTCCT
NM_003941.3       CCACCATCAAGGGGAGGGCCACCTCCTCCTCCTCCCCCTCCACACAACTCAGGTCCTCCT
                  ************************************************************

HsCD00512949      CCTCCTCCTGCTAGGGGAAGAGGCGCTCCTCCCCCACCACCTTCAAGAGCTCCCACAGCT
NM_003941.3       CCTCCTCCTGCTAGGGGAAGAGGCGCTCCTCCCCCACCACCTTCAAGAGCTCCCACAGCT
                  ************************************************************

HsCD00512949      GCACCTCCACCACCGCCTCCTTCCAGGCCAAGTGTAGCAGTCCCTCCACCACCGCCAAAT
NM_003941.3       GCACCTCCACCACCGCCTCCTTCCAGGCCAAGTGTAGCAGTCCCTCCACCACCGCCAAAT
                  ************************************************************

HsCD00512949      AGGATGTACCCTCCTCCACCTCCAGCCCTTCCCTCCTCAGCACCTTCAGGGCCTCCACCA
NM_003941.3       AGGATGTACCCTCCTCCACCTCCAGCCCTTCCCTCCTCAGCACCTTCAGGGCCTCCACCA
                  ************************************************************

HsCD00512949      CCACCTCCATCTGTGTTGGGGGTAGGGCCAGTGGCACCACCCCCACCGCCTCCACCTCCC
NM_003941.3       CCACCTCCATCTGTGTTGGGGGTAGGGCCAGTGGCACCACCCCCACCGCCTCCACCTCCA
                  ***********************************************************.

HsCD00512949      CCTCCTCCTGGGCCACCGCCCCCGCCTGGCCTGCCTTCTGATGGGGACCATCAGGTTCCA
NM_003941.3       CCTCCTCCTGGGCCACCGCCCCCGCCTGGCCTGCCTTCTGATGGGGACCATCAGGTTCCA
                  ************************************************************

HsCD00512949      ACTACTGCAGGAAACAAAGCAGCTCTTTTAGATCAAATTAGAGAGGGTGCTCAGCTAAAA
NM_003941.3       ACTACTGCAGGAAACAAAGCAGCTCTTTTAGATCAAATTAGAGAGGGTGCTCAGCTAAAA
                  ************************************************************

HsCD00512949      AAAGTGGAGCAGAACAGTCGGCCAGTGTCCTGCTCTGGACGAGATGCACTGTTAGACCAG
NM_003941.3       AAAGTGGAGCAGAACAGTCGGCCAGTGTCCTGCTCTGGACGAGATGCACTGTTAGACCAG
                  ************************************************************

HsCD00512949      ATACGACAGGGTATCCAACTAAAATCTGTGGCTGATGGCCAAGAGTCTACACCACCAACA
NM_003941.3       ATACGACAGGGTATCCAACTAAAATCTGTGGCTGATGGCCAAGAGTCTACACCACCAACA
                  ************************************************************

HsCD00512949      CCTGCACCCACTTCAGGAATTGTGGGTGCATTAATGGAAGTGATGCAGAAAAGGAGCAAA
NM_003941.3       CCTGCACCCACTTCAGGAATTGTGGGTGCATTAATGGAAGTGATGCAGAAAAGGAGCAAA
                  ************************************************************

HsCD00512949      GCCATTCATTCTTCAGATGAAGATGAAGATGAAGATGATGAAGAAGATTTTGAGGATGAT
NM_003941.3       GCCATTCATTCTTCAGATGAAGATGAAGATGAAGATGATGAAGAAGATTTTGAGGATGAT
                  ************************************************************

HsCD00512949      GATGAGTGGGAAGAC---
NM_003941.3       GATGAGTGGGAAGACTGA
                  ***************

Pathways

NCI :

CDC42 signaling events

NCI :

EGF receptor (ErbB1) signaling pathway

NCI :

EPHB forward signaling

NCI :

ErbB1 downstream signaling

NCI :

Nephrin/Neph1 signaling in the kidney podocyte

NCI :

Netrin-mediated signaling events

NCI :

PDGFR-beta signaling pathway

NCI :

Signaling events mediated by focal adhesion kinase

NCI :

Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)

Panther :

Cytoskeletal regulation by Rho GTPase

Panther :

Huntington disease

Reactome :

Axon guidance

Reactome :

Cell-Cell communication

Reactome :

DCC mediated attractive signaling

Reactome :

Developmental Biology

Reactome :

eNOS activation and regulation

Reactome :

Fcgamma receptor (FCGR) dependent phagocytosis

Reactome :

Immune System

Reactome :

Innate Immune System

Reactome :

Metabolism

Reactome :

Metabolism of nitric oxide

Reactome :

Nephrin interactions

Reactome :

Netrin-1 signaling

Reactome :

NOSTRIN mediated eNOS trafficking

Reactome :

Regulation of actin dynamics for phagocytic cup formation

WikiPathway :

G13 Signaling Pathway

Protein

SMART domain :

WH1 : WASP homology region 1

SMART domain :

WH2 : Wiskott Aldrich syndrome homology region 2

SMART domain :

PBD : P21-Rho-binding domain

HPRD :

05448

Clone

Cloning Information :

669920

Original Clone ID :

ccsbBroadEn_07334

Organism

Species Specific ID:

8976

Genome

Chromosome :

Map Location :

7q31.3

Ensembl :

ENSG00000106299

Reagents

Labome :

WASL-antibody