DNASU Plasmid | USP22 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: USP22

Gene Name: ubiquitin specific peptidase 22

Original Clone ID:

ccsbBroadEn_11719

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC126898.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513050

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1540nts

Open reading frame: 1 to 1540

Reference Sequence Alignment

ATGGCGCCGGGTTGGCCCTCACTATCAGCGGGCTCCCGACAGGAGGCGCCCCAGCTTGCG
GCCGGGGGCAGCGCCTACCAGGCAGTTGGCAGGCAGTTCCAGCCCCGGGCCACGGCACTG
CAGGGCCCGAGCCAGGCCAAGTCCTGTATCTGCCATGTCTGTGGCGTCCACCTCAACAGG
CTGCATTCCTGCCTCTACTGTGTCTTCTTCGGCTGTTTAACAAAGAAGCATATTCACGAG
CATGCGAAGGCGAAGCGGCACAACCTGGCCATTGATCTGATGTATGGAGGCATCTACTGT
TTTCTGTGCCAGGACTACATCTATGACAAAGACATGGAAATAATCGCCAAGGAGGAGCAG
CGAAAAGCTTGGAAAATGCAAGGCGTTGGAGAGAAGTTTTCAACTTGGGAACCAACCAAA
CGGGAGCTTGAACTGCTGAAGCACAACCCGAAAAGGAGAAAGATCACCTCGAACTGCACC
ATAGGTCTGCGTGGGCTGATCAACCTTGGGAACACATGCTTCATGAACTGCATCGTGCAG
GCCCTGACCCACACGCCACTTCTGCGGGACTTCTTCCTGTCTGACAGGCACCGCTGTGAG
ATGCAGAGCCCCAGCTCCTGTCTGGTCTGTGAGATGTCCTCACTGTTTCAGGAGTTTTAC
TCTGGACACCGGTCCCCTCACATCCCGTATAAGTTGCTGCACCTGGTGTGGACCCACGCG
AGGCACCTAGCAGGCTACGAGCAGCAGGACGCCCACGAGTTCCTCATCGCGGCCCTGGAC
GTGCTCCACCGACACTGCAAAGGTGATGACAATGGGAAGAAGGCCAACAACCCCAACCAC
TGCAACTGCATCATAGACCAGATCTTCACAGGCGGGTTGCAGTCAGACGTCACCTGCCAA
GTCTGCCATGGAGTCTCCACCACCATCGACCCCTTCTGGGACATCAGCTTGGATCTCCCC
GGCTCTTCCACCCCATTCTGGCCCCTGAGCCCAGGGAGCGAGGGCAACGTGGTAAACGGG
GAAAGCCACGTGTCGGGAACCACCACGCTCACGGACTGCCTGCGACGATTCACCAGACCA
GAGCACTTGGGCAGCAGCGCCAAGATCAAGTGCAGCGGTTGCCATAGCTACCAGGAGTCC
ACAAAGCAGCTCACTATGAAGAAACTGCCCATCGTAGCCTGTTTTCATCTCAAACGATTT
GAACACTCAGCCAAGCTGCGGCGGAAGATCACCACGTATGTGTCCTTCCCCCTGGAGCTG
GACATGACCCCTTTCATGGCCTCCAGCAAAGAGAGCAGGATGAATGGACAGTACCAGCAG
CCCACGGACAGTCTCAACAATGACAACAAGTATTCCCTGTTTGCTGTTGTTAACCATCAA
GGGACCTTGGAGAGTGGCCACTACACCAGCTTTATCCGGCAGCACAAAGACCAGTGGTTC
AAGTGTGACGATGCCATCATCACCAAGGCCAGCATCAAGGACGTCCTGGACAGCGAAGGG
TACTTGCTGTTCTATCACAAACAGTTCCTGGAATACGAG

Protein sequence predicted based on nucleotide sequence

MAPGWPSLSAGSRQEAPQLAAGGSAYQAVGRQFQPRATALQGPSQAKSCICHVCGVHLNR
LHSCLYCVFFGCLTKKHIHEHAKAKRHNLAIDLMYGGIYCFLCQDYIYDKDMEIIAKEEQ
RKAWKMQGVGEKFSTWEPTKRELELLKHNPKRRKITSNCTIGLRGLINLGNTCFMNCIVQ
ALTHTPLLRDFFLSDRHRCEMQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHA
RHLAGYEQQDAHEFLIAALDVLHRHCKGDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQ
VCHGVSTTIDPFWDISLDLPGSSTPFWPLSPGSEGNVVNGESHVSGTTTLTDCLRRFTRP
EHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSAKLRRKITTYVSFPLEL
DMTPFMASSKESRMNGQYQQPTDSLNNDNKYSLFAVVNHQGTLESGHYTSFIRQHKDQWF
KCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

USP22

Symbol Nomenclature :

USP22

SYNONYM :

USP3L

Designation :

Full Nomenclature :

ubiquitin specific peptidase 22

GENEID :

23326

HGNC :

12621

MIM :

612116

Vega :

OTTHUMG00000133621

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00513050      ------------------------------------ATGGCGCCGGGTTGGCCCTCACTA
NM_015276.1       ATGGTGTCCCGGCCAGAGCCCGAGGGCGAGGCCATGGACGCCGAGCTGGCGGTAGCGCCG
                                                      .: **  .*     *  . *.* .

HsCD00513050      TCAGCGGGCTCCCGACAGGAGGCGCCCCAGCTTGCGGCCGGGGGCAGCGCCTACCAGGCA
NM_015276.1       CCGGGCTGCTCGCACCTGGGCAGCTTCAAGGTGGACAACTGGAAGCAGAACCTGCGGGCC
                   *.*   **** *..*:**. .    *.** * *. ..* **.. .. ..* : *.***.

HsCD00513050      GTTGGCAGGCAGTTCCAGCCCCGGGCCACGGCACTGCAGGGCCCGAGCCAGGCCAAGTCC
NM_015276.1       ATCTACCAGTGCTTCGTGTGGAGCGGCACGGCTGAGGCCCGCAAGCGCAAGGCCAAGTCC
                  .*  .*..* . *** :*   .* * ******: :* .  **..*.**.***********

HsCD00513050      TGTATCTGCCATGTCTGTGGCGTCCACCTCAACAGGCTGCATTCCTGCCTCTACTGTGTC
NM_015276.1       TGTATCTGCCATGTCTGTGGCGTCCACCTCAACAGGCTGCATTCCTGCCTCTACTGTGTC
                  ************************************************************

HsCD00513050      TTCTTCGGCTGTTTAACAAAGAAGCATATTCACGAGCATGCGAAGGCGAAGCGGCACAAC
NM_015276.1       TTCTTCGGCTGTTTCACAAAGAAGCATATTCACGAGCATGCGAAGGCGAAGCGGCACAAC
                  **************.*********************************************

HsCD00513050      CTGGCCATTGATCTGATGTATGGAGGCATCTACTGTTTTCTGTGCCAGGACTACATCTAT
NM_015276.1       CTGGCCATTGATCTGATGTACGGAGGCATCTACTGTTTTCTGTGCCAGGACTACATCTAT
                  ******************** ***************************************

HsCD00513050      GACAAAGACATGGAAATAATCGCCAAGGAGGAGCAGCGAAAAGCTTGGAAAATGCAAGGC
NM_015276.1       GACAAAGACATGGAAATAATCGCCAAGGAGGAGCAGCGAAAAGCTTGGAAAATGCAAGGC
                  ************************************************************

HsCD00513050      GTTGGAGAGAAGTTTTCAACTTGGGAACCAACCAAACGGGAGCTTGAACTGCTGAAGCAC
NM_015276.1       GTTGGAGAGAAGTTTTCAACTTGGGAACCAACCAAACGGGAGCTTGAACTGCTGAAGCAC
                  ************************************************************

HsCD00513050      AACCCGAAAAGGAGAAAGATCACCTCGAACTGCACCATAGGTCTGCGTGGGCTGATCAAC
NM_015276.1       AACCCGAAAAGGAGAAAGATCACCTCGAACTGCACCATAGGTCTGCGTGGGCTGATCAAC
                  ************************************************************

HsCD00513050      CTTGGGAACACATGCTTCATGAACTGCATCGTGCAGGCCCTGACCCACACGCCACTTCTG
NM_015276.1       CTTGGGAACACATGCTTCATGAACTGCATCGTGCAGGCCCTGACCCACACGCCACTTCTG
                  ************************************************************

HsCD00513050      CGGGACTTCTTCCTGTCTGACAGGCACCGCTGTGAGATGCAGAGCCCCAGCTCCTGTCTG
NM_015276.1       CGGGACTTCTTCCTGTCTGACAGGCACCGCTGTGAGATGCAGAGCCCCAGCTCCTGTCTG
                  ************************************************************

HsCD00513050      GTCTGTGAGATGTCCTCACTGTTTCAGGAGTTTTACTCTGGACACCGGTCCCCTCACATC
NM_015276.1       GTCTGTGAGATGTCCTCACTGTTTCAGGAGTTTTACTCTGGACACCGGTCCCCTCACATC
                  ************************************************************

HsCD00513050      CCGTATAAGTTGCTGCACCTGGTGTGGACCCACGCGAGGCACCTAGCAGGCTACGAGCAG
NM_015276.1       CCGTATAAGTTGCTGCACCTGGTGTGGACCCACGCGAGGCACCTAGCAGGCTACGAGCAG
                  ************************************************************

HsCD00513050      CAGGACGCCCACGAGTTCCTCATCGCGGCCCTGGACGTGCTCCACCGACACTGCAAAGGT
NM_015276.1       CAGGACGCCCACGAGTTCCTCATCGCGGCCCTGGACGTGCTCCACCGACACTGCAAAGGT
                  ************************************************************

HsCD00513050      GATGACAATGGGAAGAAGGCCAACAACCCCAACCACTGCAACTGCATCATAGACCAGATC
NM_015276.1       GATGACAATGGGAAGAAGGCCAACAACCCCAACCACTGCAACTGCATCATAGACCAGATC
                  ************************************************************

HsCD00513050      TTCACAGGCGGGTTGCAGTCAGACGTCACCTGCCAAGTCTGCCATGGAGTCTCCACCACC
NM_015276.1       TTCACAGGCGGGTTGCAGTCAGACGTCACCTGCCAAGTCTGCCATGGAGTCTCCACCACC
                  ************************************************************

HsCD00513050      ATCGACCCCTTCTGGGACATCAGCTTGGATCTCCCCGGCTCTTCCACCCCATTCTGGCCC
NM_015276.1       ATCGACCCCTTCTGGGACATCAGCTTGGATCTCCCCGGCTCTTCCACCCCATTCTGGCCC
                  ************************************************************

HsCD00513050      CTGAGCCCAGGGAGCGAGGGCAACGTGGTAAACGGGGAAAGCCACGTGTCGGGAACCACC
NM_015276.1       CTGAGCCCAGGGAGCGAGGGCAACGTGGTAAACGGGGAAAGCCACGTGTCGGGAACCACC
                  ************************************************************

HsCD00513050      ACGCTCACGGACTGCCTGCGACGATTCACCAGACCAGAGCACTTGGGCAGCAGCGCCAAG
NM_015276.1       ACGCTCACGGACTGCCTGCGACGATTCACCAGACCAGAGCACTTGGGCAGCAGCGCCAAG
                  ************************************************************

HsCD00513050      ATCAAGTGCAGCGGTTGCCATAGCTACCAGGAGTCCACAAAGCAGCTCACTATGAAGAAA
NM_015276.1       ATCAAGTGCAGCGGTTGCCATAGCTACCAGGAGTCCACAAAGCAGCTCACTATGAAGAAA
                  ************************************************************

HsCD00513050      CTGCCCATCGTAGCCTGTTTTCATCTCAAACGATTTGAACACTCAGCCAAGCTGCGGCGG
NM_015276.1       CTGCCCATCGTAGCCTGTTTTCATCTCAAACGATTTGAACACTCAGCCAAGCTGCGGCGG
                  ************************************************************

HsCD00513050      AAGATCACCACGTATGTGTCCTTCCCCCTGGAGCTGGACATGACCCCTTTCATGGCCTCC
NM_015276.1       AAGATCACCACGTATGTGTCCTTCCCCCTGGAGCTGGACATGACCCCTTTCATGGCCTCC
                  ************************************************************

HsCD00513050      AGCAAAGAGAGCAGGATGAATGGACAGTACCAGCAGCCCACGGACAGTCTCAACAATGAC
NM_015276.1       AGCAAAGAGAGCAGGATGAATGGACAGTACCAGCAGCCCACGGACAGTCTCAACAATGAC
                  ************************************************************

HsCD00513050      AACAAGTATTCCCTGTTTGCTGTTGTTAACCATCAAGGGACCTTGGAGAGTGGCCACTAC
NM_015276.1       AACAAGTATTCCCTGTTTGCTGTTGTTAACCATCAAGGGACCTTGGAGAGTGGCCACTAC
                  ************************************************************

HsCD00513050      ACCAGCTTTATCCGGCAGCACAAAGACCAGTGGTTCAAGTGTGACGATGCCATCATCACC
NM_015276.1       ACCAGCTTTATCCGGCAGCACAAAGACCAGTGGTTCAAGTGTGACGATGCCATCATCACC
                  ************************************************************

HsCD00513050      AAGGCCAGCATCAAGGACGTCCTGGACAGCGAAGGGTACTTGCTGTTCTATCACAAACAG
NM_015276.1       AAGGCCAGCATCAAGGACGTCCTGGACAGCGAAGGGTACTTGCTGTTCTATCACAAACAG
                  ************************************************************

HsCD00513050      TTCCTGGAATACGAG---
NM_015276.1       TTCCTGGAATACGAGTAG
                  ***************