DNASU Plasmid | SMU1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: SMU1

Gene Name: smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans)

Original Clone ID:

ccsbBroadEn_03556

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC002876.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513080

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1540nts

Open reading frame: 1 to 1540

Reference Sequence Alignment

ATGTCGATCGAAATCGAATCTTCGGATGTGATCCGCCTTATTATGCAGTACTTGAAGGAG
AACAGTTTACATCGGGCGTTAGCCACCTTGCAGGAGGAGACTACTGTGTCTCTGAATACT
GTGGACAGCATTGAGAGTTTTGTGGCTGACATTAACAGTGGCCATTGGGATACTGTGTTG
CAGGCTATACAGTCTCTGAAATTGCCAGACAAAACCCTCATTGACCTCTATGAACAGGTT
GTTCTGGAATTGATAGAGCTCCGTGAATTGGGTGCTGCCAGGTCACTTTTGAGACAGACT
GATCCCATGATCATGTTAAAACAAACACAGCCAGAGCGATATATTCATCTGGAGAACCTT
TTGGCCAGGTCTTACTTTGATCCTCGTGAGGCATACCCAGATGGAAGTAGCAAAGAAAAG
AGAAGAGCAGCAATTGCCCAGGCCTTAGCTGGCGAAGTCAGTGTGGTGCCTCCATCTCGT
CTCATGGCATTGCTGGGACAGGCACTGAAGTGGCAGCAGCATCAGGGATTGCTTCCTCCT
GGTATGACCATAGATTTGTTTCGAGGCAAGGCAGCTGTCAAAGATGTGGAAGAAGAAAAG
TTTCCTACACAACTGAGCAGGCATATTAAGTTTGGTCAGAAATCACATGTGGAGTGTGCT
CGATTTTCTCCAGATGGTCAGTATTTGGTCACTGGGTCTGTTGATGGATTCATTGAAGTA
TGGAACTTTACTACTGGAAAAATCAGAAAGGATCTTAAGTACCAGGCCCAAGATAACTTT
ATGATGATGGATGATGCTGTCCTCTGCATGTGTTTCAGCAGAGATACAGAAATGTTAGCA
ACTGGGGCCCAAGATGGAAAAATCAAGGTGTGGAAGATTCAGAGTGGACAATGTTTAAGG
AGATTTGAGAGGGCACACAGTAAGGGTGTCACCTGTCTAAGCTTTTCTAAGGATAGCAGT
CAGATCCTTAGTGCTTCTTTTGACCAGACAATTAGAATTCATGGTTTAAAATCTGGGAAA
ACCCTGAAGGAATTTCGTGGCCATTCCTCCTTTGTTAACGAAGCAACATTTACACAAGAT
GGACATTACATTATTAGTGCATCCTCTGATGGCACTGTAAAGATCTGGAATATGAAGACC
ACAGAATGTTCAAATACCTTTAAATCCCTGGGCAGCACCGCAGGGACAGATATTACCGTC
AACAGTGTGATTCTACTTCCTAAAAACCCTGAGCACTTTGTGGTGTGCAACAGATCAAAC
ACGGTGGTCATCATGAACATGCAGGGGCAGATTGTCAGAAGCTTCAGTTCTGGTAAAAGA
GAAGGTGGGGACTTTGTTTGCTGTGCCCTCTCTCCCCGTGGTGAATGGATCTACTGTGTA
GGGGAGGACTTTGTGCTCTACTGTTTCAGTACAGTCACTGGCAAACTGGAGAGAACTTTG
ACAGTGCACGAGAAGGATGTGATTGGTATTGCACATCACCCTCATCAGAACCTGATTGCT
ACCTACAGTGAAGATGGACTCCTAAAGCTCTGGAAACCA

Protein sequence predicted based on nucleotide sequence

MSIEIESSDVIRLIMQYLKENSLHRALATLQEETTVSLNTVDSIESFVADINSGHWDTVL
QAIQSLKLPDKTLIDLYEQVVLELIELRELGAARSLLRQTDPMIMLKQTQPERYIHLENL
LARSYFDPREAYPDGSSKEKRRAAIAQALAGEVSVVPPSRLMALLGQALKWQQHQGLLPP
GMTIDLFRGKAAVKDVEEEKFPTQLSRHIKFGQKSHVECARFSPDGQYLVTGSVDGFIEV
WNFTTGKIRKDLKYQAQDNFMMMDDAVLCMCFSRDTEMLATGAQDGKIKVWKIQSGQCLR
RFERAHSKGVTCLSFSKDSSQILSASFDQTIRIHGLKSGKTLKEFRGHSSFVNEATFTQD
GHYIISASSDGTVKIWNMKTTECSNTFKSLGSTAGTDITVNSVILLPKNPEHFVVCNRSN
TVVIMNMQGQIVRSFSSGKREGGDFVCCALSPRGEWIYCVGEDFVLYCFSTVTGKLERTL
TVHEKDVIGIAHHPHQNLIATYSEDGLLKLWKP

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

SMU1

Symbol Nomenclature :

SMU1

SYNONYM :

BWD; RP11-54K16.3; SMU-1; fSAP57

Designation :

WD40 repeat-containing protein SMU1|brain-enriched WD-repeat protein|functional spliceosome-associated protein 57|smu-1 suppressor of mec-8 and unc-52 protein homolog

Full Nomenclature :

smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans)

GENEID :

55234

GenBank Accession :

DQ896251

HGNC :

18247

Vega :

OTTHUMG00000019757

Target GenBank:

DQ896251

Reference Sequence Alignment

  

HsCD00513080      ATGTCGATCGAAATCGAATCTTCGGATGTGATCCGCCTTATTATGCAGTACTTGAAGGAG
NM_018225.2       ATGTCGATCGAAATCGAATCTTCGGATGTGATCCGCCTTATTATGCAGTACTTGAAGGAG
                  ************************************************************

HsCD00513080      AACAGTTTACATCGGGCGTTAGCCACCTTGCAGGAGGAGACTACTGTGTCTCTGAATACT
NM_018225.2       AACAGTTTACATCGGGCGTTAGCCACCTTGCAGGAGGAGACTACTGTGTCTCTGAATACT
                  ************************************************************

HsCD00513080      GTGGACAGCATTGAGAGTTTTGTGGCTGACATTAACAGTGGCCATTGGGATACTGTGTTG
NM_018225.2       GTGGACAGCATTGAGAGTTTTGTGGCTGACATTAACAGTGGCCATTGGGATACTGTGTTG
                  ************************************************************

HsCD00513080      CAGGCTATACAGTCTCTGAAATTGCCAGACAAAACCCTCATTGACCTCTATGAACAGGTT
NM_018225.2       CAGGCTATACAGTCTCTGAAATTGCCAGACAAAACCCTCATTGACCTCTATGAACAGGTT
                  ************************************************************

HsCD00513080      GTTCTGGAATTGATAGAGCTCCGTGAATTGGGTGCTGCCAGGTCACTTTTGAGACAGACT
NM_018225.2       GTTCTGGAATTGATAGAGCTCCGTGAATTGGGTGCTGCCAGGTCACTTTTGAGACAGACT
                  ************************************************************

HsCD00513080      GATCCCATGATCATGTTAAAACAAACACAGCCAGAGCGATATATTCATCTGGAGAACCTT
NM_018225.2       GATCCCATGATCATGTTAAAACAAACACAGCCAGAGCGATATATTCATCTGGAGAACCTT
                  ************************************************************

HsCD00513080      TTGGCCAGGTCTTACTTTGATCCTCGTGAGGCATACCCAGATGGAAGTAGCAAAGAAAAG
NM_018225.2       TTGGCCAGGTCTTACTTTGATCCTCGTGAGGCATACCCAGATGGAAGTAGCAAAGAAAAG
                  ************************************************************

HsCD00513080      AGAAGAGCAGCAATTGCCCAGGCCTTAGCTGGCGAAGTCAGTGTGGTGCCTCCATCTCGT
NM_018225.2       AGAAGAGCAGCAATTGCCCAGGCCTTAGCTGGCGAAGTCAGTGTGGTGCCTCCATCTCGT
                  ************************************************************

HsCD00513080      CTCATGGCATTGCTGGGACAGGCACTGAAGTGGCAGCAGCATCAGGGATTGCTTCCTCCT
NM_018225.2       CTCATGGCATTGCTGGGACAGGCACTGAAGTGGCAGCAGCATCAGGGATTGCTTCCTCCT
                  ************************************************************

HsCD00513080      GGTATGACCATAGATTTGTTTCGAGGCAAGGCAGCTGTCAAAGATGTGGAAGAAGAAAAG
NM_018225.2       GGTATGACCATAGATTTGTTTCGAGGCAAGGCAGCTGTCAAAGATGTGGAAGAAGAAAAG
                  ************************************************************

HsCD00513080      TTTCCTACACAACTGAGCAGGCATATTAAGTTTGGTCAGAAATCACATGTGGAGTGTGCT
NM_018225.2       TTTCCTACACAACTGAGCAGGCATATTAAGTTTGGTCAGAAATCACATGTGGAGTGTGCT
                  ************************************************************

HsCD00513080      CGATTTTCTCCAGATGGTCAGTATTTGGTCACTGGGTCTGTTGATGGATTCATTGAAGTA
NM_018225.2       CGATTTTCTCCAGATGGTCAGTATTTGGTCACTGGGTCTGTTGATGGATTCATTGAAGTA
                  ************************************************************

HsCD00513080      TGGAACTTTACTACTGGAAAAATCAGAAAGGATCTTAAGTACCAGGCCCAAGATAACTTT
NM_018225.2       TGGAACTTTACTACTGGAAAAATCAGAAAGGATCTTAAGTACCAGGCCCAAGATAACTTT
                  ************************************************************

HsCD00513080      ATGATGATGGATGATGCTGTCCTCTGCATGTGTTTCAGCAGAGATACAGAAATGTTAGCA
NM_018225.2       ATGATGATGGATGATGCTGTCCTCTGCATGTGTTTCAGCAGAGATACAGAAATGTTAGCA
                  ************************************************************

HsCD00513080      ACTGGGGCCCAAGATGGAAAAATCAAGGTGTGGAAGATTCAGAGTGGACAATGTTTAAGG
NM_018225.2       ACTGGGGCCCAAGATGGAAAAATCAAGGTGTGGAAGATTCAGAGTGGACAATGTTTAAGG
                  ************************************************************

HsCD00513080      AGATTTGAGAGGGCACACAGTAAGGGTGTCACCTGTCTAAGCTTTTCTAAGGATAGCAGT
NM_018225.2       AGATTTGAGAGGGCACACAGTAAGGGTGTCACCTGTCTAAGCTTTTCTAAGGATAGCAGT
                  ************************************************************

HsCD00513080      CAGATCCTTAGTGCTTCTTTTGACCAGACAATTAGAATTCATGGTTTAAAATCTGGGAAA
NM_018225.2       CAGATCCTTAGTGCTTCTTTTGACCAGACAATTAGAATTCATGGTTTAAAATCTGGGAAA
                  ************************************************************

HsCD00513080      ACCCTGAAGGAATTTCGTGGCCATTCCTCCTTTGTTAACGAAGCAACATTTACACAAGAT
NM_018225.2       ACCCTGAAGGAATTTCGTGGCCATTCCTCCTTTGTTAACGAAGCAACATTTACACAAGAT
                  ************************************************************

HsCD00513080      GGACATTACATTATTAGTGCATCCTCTGATGGCACTGTAAAGATCTGGAATATGAAGACC
NM_018225.2       GGACATTACATTATTAGTGCATCCTCTGATGGCACTGTAAAGATCTGGAATATGAAGACC
                  ************************************************************

HsCD00513080      ACAGAATGTTCAAATACCTTTAAATCCCTGGGCAGCACCGCAGGGACAGATATTACCGTC
NM_018225.2       ACAGAATGTTCAAATACCTTTAAATCCCTGGGCAGCACCGCAGGGACAGATATTACCGTC
                  ************************************************************

HsCD00513080      AACAGTGTGATTCTACTTCCTAAAAACCCTGAGCACTTTGTGGTGTGCAACAGATCAAAC
NM_018225.2       AACAGTGTGATTCTACTTCCTAAAAACCCTGAGCACTTTGTGGTGTGCAACAGATCAAAC
                  ************************************************************

HsCD00513080      ACGGTGGTCATCATGAACATGCAGGGGCAGATTGTCAGAAGCTTCAGTTCTGGTAAAAGA
NM_018225.2       ACGGTGGTCATCATGAACATGCAGGGGCAGATTGTCAGAAGCTTCAGTTCTGGTAAAAGA
                  ************************************************************

HsCD00513080      GAAGGTGGGGACTTTGTTTGCTGTGCCCTCTCTCCCCGTGGTGAATGGATCTACTGTGTA
NM_018225.2       GAAGGTGGGGACTTTGTTTGCTGTGCCCTCTCTCCCCGTGGTGAATGGATCTACTGTGTA
                  ************************************************************

HsCD00513080      GGGGAGGACTTTGTGCTCTACTGTTTCAGTACAGTCACTGGCAAACTGGAGAGAACTTTG
NM_018225.2       GGGGAGGACTTTGTGCTCTACTGTTTCAGTACAGTCACTGGCAAACTGGAGAGAACTTTG
                  ************************************************************

HsCD00513080      ACAGTGCACGAGAAGGATGTGATTGGTATTGCACATCACCCTCATCAGAACCTGATTGCT
NM_018225.2       ACAGTGCACGAGAAGGATGTGATTGGTATTGCACATCACCCTCATCAGAACCTGATTGCT
                  ************************************************************

HsCD00513080      ACCTACAGTGAAGATGGACTCCTAAAGCTCTGGAAACCA---
NM_018225.2       ACCTACAGTGAAGATGGACTCCTAAAGCTCTGGAAACCATAA
                  ***************************************