DNASU Plasmid | DEPDC1B (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: DEPDC1B

Gene Name: DEP domain containing 1B

Original Clone ID:

ccsbBroadEn_08587

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC019075.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513125

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1588nts

Open reading frame: 1 to 1588

Reference Sequence Alignment

ATGGAGCATCGCATCGTGGGGCCCGGGCCGTACCGAGCTACCAGGCTGTGGAATGAGACC
GTGGAGCTTTTTCGTGCTAAGATGCCGTTACGGAAACATCGCTGTCGTTTCAAGAGCTAT
GAGCATTGTTTCACAGCGGCCGAAGCTGTGGATTGGCTGCATGAGCTGCTGAGGTGCAGT
CAAAACTTCGGCCCTGAAGTGACCCGCAAACAAACGGTCCAGCTGCTAAAAAAATTCCTG
AAGAATCACGTTATTGAAGACATCAAGGGAAAATGGGGTGAGGAAGATTTTGAAGACAAT
CGTCACTTATACAGATTTCCTCCTTCTTCACCCCTGAAACCATATCCAAAGAAGCCCCCA
AACCAAAAGGATGTTATTAAATTTCCAGAATGGAATGATCTCCCACCAGGCACTTCACAA
GAGAACATCCCAGTGAGGCCAGTTGTGATGAATTCTGAGATGTGGTACAAGCGTCACAGT
ATTGCAATTGGAGAGGTGCCAGCTTGCCGTCTTGTCCACCGCAGACAGCTGACAGAGGCC
AATGTAGAAGAGATATGGAAGTCTATGACATTATCATACTTACAGAAAATTCTTGGCCTG
GATTCCTTAGAAGAAGTTTTAGACGTCAAACTTGTCAATTCGAAGTTCATCATCCATAAT
GTATATAGTGTTAGCAAGCAGGGAGTTGTTATTCTTGATGACAAGTCAAAAGAACTTCCT
CATTGGGTGCTGTCAGCTATGAAGTGTTTGGCAAATTGGCCCAACTGTTCTGATTTGAAG
CAGCCTATGTACTTGGGATTTGAAAAAGATGTCTTTAAAACCATAGCTGATTACTATGGT
CACTTGAAAGAGCCTCTACTTACATTTCATCTTTTTGATGCTTTTGTCAGTGTACTGGGT
TTGTTACAGAAGGAGAAAGTGGCAGTTGAAGCATTTCAGATTTGCTGCCTTCTCCTACCT
CCTGAAAATAGGAGAAAGTTACAGCTATTGATGATGATGATGGCAAGGATTTGCTTAAAC
AAAGAGATGCCACCCCTGTGTGATGGCTTTGGTACCCGAACACTGATGGTTCAGACATTT
TCCCGTTGCATCTTGTGTTCCAAGGATGAAGTGGACTTGGATGAGTTATTAGCTGCTAGA
TTGGTAACGTTTCTGATGGACAATTACCAGGAAATTCTGAAACTCCCTTTGGCCTTGCAG
ACCTCTATAGAGGAGCGTGTGGCTCATCTACGAAGAGTCCAGATAAAATACCCAGGAGCT
GATATGGATATCACTTTATCTGCTCCATCATTTTGCCGTCAAATTAGTCCAGAGGAATTT
GAATATCAAAGATCATATGGCTCTCAGGAACCTCTGGCAGCCTTGTTGGAGGAAGTCATA
ACAGATGCCAAACTCTCCAACAAAGAGAAAAAGAAGAAACTGAAGCAGTTTCAGAAATCC
TATCCTGAAGTCTATCAAGAACGATTTCCTACACCAGAAAGTGCAGCACTTCTGTTTCCT
GAAAAACCCAAACCGAAACCACAGCTGCTAATGTGGGCACTAAAGAAGCCTTTCCAACCA
TTTCAAAGAACTAGAAGTTTTCGAATG

Protein sequence predicted based on nucleotide sequence

MEHRIVGPGPYRATRLWNETVELFRAKMPLRKHRCRFKSYEHCFTAAEAVDWLHELLRCS
QNFGPEVTRKQTVQLLKKFLKNHVIEDIKGKWGEEDFEDNRHLYRFPPSSPLKPYPKKPP
NQKDVIKFPEWNDLPPGTSQENIPVRPVVMNSEMWYKRHSIAIGEVPACRLVHRRQLTEA
NVEEIWKSMTLSYLQKILGLDSLEEVLDVKLVNSKFIIHNVYSVSKQGVVILDDKSKELP
HWVLSAMKCLANWPNCSDLKQPMYLGFEKDVFKTIADYYGHLKEPLLTFHLFDAFVSVLG
LLQKEKVAVEAFQICCLLLPPENRRKLQLLMMMMARICLNKEMPPLCDGFGTRTLMVQTF
SRCILCSKDEVDLDELLAARLVTFLMDNYQEILKLPLALQTSIEERVAHLRRVQIKYPGA
DMDITLSAPSFCRQISPEEFEYQRSYGSQEPLAALLEEVITDAKLSNKEKKKKLKQFQKS
YPEVYQERFPTPESAALLFPEKPKPKPQLLMWALKKPFQPFQRTRSFRM

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

DEPDC1B

Symbol Nomenclature :

DEPDC1B

SYNONYM :

BRCC3; XTP1

Designation :

DEP domain-containing protein 1B|HBV X-transactivated gene 8 protein|HBV XAg-transactivated protein 8|HBxAg transactivated protein 1|breast cancer cell 3

Full Nomenclature :

DEP domain containing 1B

GENEID :

55789

HGNC :

24902

Vega :

OTTHUMG00000097083

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00513125      ATGGAGCATCGCATCGTGGGGCCCGGGCCGTACCGAGCTACCAGGCTGTGGAATGAGACC
NM_018369.2       ATGGAGCATCGCATCGTGGGGCCCGGGCCGTACCGAGCTACCAGGCTGTGGAATGAGACC
                  ************************************************************

HsCD00513125      GTGGAGCTTTTTCGTGCTAAGATGCCGTTACGGAAACATCGCTGTCGTTTCAAGAGCTAT
NM_018369.2       GTGGAGCTTTTTCGTGCTAAGATGCCGTTACGGAAACATCGCTGTCGTTTCAAGAGCTAT
                  ************************************************************

HsCD00513125      GAGCATTGTTTCACAGCGGCCGAAGCTGTGGATTGGCTGCATGAGCTGCTGAGGTGCAGT
NM_018369.2       GAGCATTGTTTCACAGCGGCCGAAGCTGTGGATTGGCTGCATGAGCTGCTGAGGTGCAGT
                  ************************************************************

HsCD00513125      CAAAACTTCGGCCCTGAAGTGACCCGCAAACAAACGGTCCAGCTGCTAAAAAAATTCCTG
NM_018369.2       CAAAACTTCGGCCCTGAAGTGACCCGCAAACAAACGGTCCAGCTGCTAAAAAAATTCCTG
                  ************************************************************

HsCD00513125      AAGAATCACGTTATTGAAGACATCAAGGGAAAATGGGGTGAGGAAGATTTTGAAGACAAT
NM_018369.2       AAGAATCACGTTATTGAAGACATCAAGGGAAAATGGGGTGAGGAAGATTTTGAAGACAAT
                  ************************************************************

HsCD00513125      CGTCACTTATACAGATTTCCTCCTTCTTCACCCCTGAAACCATATCCAAAGAAGCCCCCA
NM_018369.2       CGTCACTTATACAGATTTCCTCCTTCTTCACCCCTGAAACCATATCCAAAGAAGCCCCCA
                  ************************************************************

HsCD00513125      AACCAAAAGGATGTTATTAAATTTCCAGAATGGAATGATCTCCCACCAGGCACTTCACAA
NM_018369.2       AACCAAAAGGATGTTATTAAATTTCCAGAATGGAATGATCTCCCACCAGGCACTTCACAA
                  ************************************************************

HsCD00513125      GAGAACATCCCAGTGAGGCCAGTTGTGATGAATTCTGAGATGTGGTACAAGCGTCACAGT
NM_018369.2       GAGAACATCCCAGTGAGGCCAGTTGTGATGAATTCTGAGATGTGGTACAAGCGTCACAGT
                  ************************************************************

HsCD00513125      ATTGCAATTGGAGAGGTGCCAGCTTGCCGTCTTGTCCACCGCAGACAGCTGACAGAGGCC
NM_018369.2       ATTGCAATTGGAGAGGTGCCAGCTTGCCGTCTTGTCCACCGCAGACAGCTGACAGAGGCC
                  ************************************************************

HsCD00513125      AATGTAGAAGAGATATGGAAGTCTATGACATTATCATACTTACAGAAAATTCTTGGCCTG
NM_018369.2       AATGTAGAAGAGATATGGAAGTCTATGACATTATCATACTTACAGAAAATTCTTGGCCTG
                  ************************************************************

HsCD00513125      GATTCCTTAGAAGAAGTTTTAGACGTCAAACTTGTCAATTCGAAGTTCATCATCCATAAT
NM_018369.2       GATTCCTTAGAAGAAGTTTTAGACGTCAAACTTGTCAATTCGAAGTTCATCATCCATAAT
                  ************************************************************

HsCD00513125      GTATATAGTGTTAGCAAGCAGGGAGTTGTTATTCTTGATGACAAGTCAAAAGAACTTCCT
NM_018369.2       GTATATAGTGTTAGCAAGCAGGGAGTTGTTATTCTTGATGACAAGTCAAAAGAACTTCCT
                  ************************************************************

HsCD00513125      CATTGGGTGCTGTCAGCTATGAAGTGTTTGGCAAATTGGCCCAACTGTTCTGATTTGAAG
NM_018369.2       CATTGGGTGCTGTCAGCTATGAAGTGTTTGGCAAATTGGCCCAACTGTTCTGATTTGAAG
                  ************************************************************

HsCD00513125      CAGCCTATGTACTTGGGATTTGAAAAAGATGTCTTTAAAACCATAGCTGATTACTATGGT
NM_018369.2       CAGCCTATGTACTTGGGATTTGAAAAAGATGTCTTTAAAACCATAGCTGATTACTATGGT
                  ************************************************************

HsCD00513125      CACTTGAAAGAGCCTCTACTTACATTTCATCTTTTTGATGCTTTTGTCAGTGTACTGGGT
NM_018369.2       CACTTGAAAGAGCCTCTACTTACATTTCATCTTTTTGATGCTTTTGTCAGTGTACTGGGT
                  ************************************************************

HsCD00513125      TTGTTACAGAAGGAGAAAGTGGCAGTTGAAGCATTTCAGATTTGCTGCCTTCTCCTACCT
NM_018369.2       TTGTTACAGAAGGAGAAAGTGGCAGTTGAAGCATTTCAGATTTGCTGCCTTCTCCTACCT
                  ************************************************************

HsCD00513125      CCTGAAAATAGGAGAAAGTTACAGCTATTGATGATGATGATGGCAAGGATTTGCTTAAAC
NM_018369.2       CCTGAAAATAGGAGAAAGTTACAGCTATTGATGAGGATGATGGCAAGGATTTGCTTAAAC
                  ********************************** *************************

HsCD00513125      AAAGAGATGCCACCCCTGTGTGATGGCTTTGGTACCCGAACACTGATGGTTCAGACATTT
NM_018369.2       AAAGAGATGCCACCCCTGTGTGATGGCTTTGGTACCCGAACACTGATGGTTCAGACATTT
                  ************************************************************

HsCD00513125      TCCCGTTGCATCTTGTGTTCCAAGGATGAAGTGGACTTGGATGAGTTATTAGCTGCTAGA
NM_018369.2       TCCCGTTGCATCTTGTGTTCCAAGGATGAAGTGGACTTGGATGAGTTATTAGCTGCTAGA
                  ************************************************************

HsCD00513125      TTGGTAACGTTTCTGATGGACAATTACCAGGAAATTCTGAAACTCCCTTTGGCCTTGCAG
NM_018369.2       TTGGTAACGTTTCTGATGGACAATTACCAGGAAATTCTGAAAGTCCCTTTGGCCTTGCAG
                  ****************************************** *****************

HsCD00513125      ACCTCTATAGAGGAGCGTGTGGCTCATCTACGAAGAGTCCAGATAAAATACCCAGGAGCT
NM_018369.2       ACCTCTATAGAGGAGCGTGTGGCTCATCTACGAAGAGTCCAGATAAAATACCCAGGAGCT
                  ************************************************************

HsCD00513125      GATATGGATATCACTTTATCTGCTCCATCATTTTGCCGTCAAATTAGTCCAGAGGAATTT
NM_018369.2       GATATGGATATCACTTTATCTGCTCCATCATTTTGCCGTCAAATTAGTCCAGAGGAATTT
                  ************************************************************

HsCD00513125      GAATATCAAAGATCATATGGCTCTCAGGAACCTCTGGCAGCCTTGTTGGAGGAAGTCATA
NM_018369.2       GAATATCAAAGATCATATGGCTCTCAGGAACCTCTGGCAGCCTTGTTGGAGGAAGTCATA
                  ************************************************************

HsCD00513125      ACAGATGCCAAACTCTCCAACAAAGAGAAAAAGAAGAAACTGAAGCAGTTTCAGAAATCC
NM_018369.2       ACAGATGCCAAACTCTCCAACAAAGAGAAAAAGAAGAAACTGAAGCAGTTTCAGAAATCC
                  ************************************************************

HsCD00513125      TATCCTGAAGTCTATCAAGAACGATTTCCTACACCAGAAAGTGCAGCACTTCTGTTTCCT
NM_018369.2       TATCCTGAAGTCTATCAAGAACGATTTCCTACACCAGAAAGTGCAGCACTTCTGTTTCCT
                  ************************************************************

HsCD00513125      GAAAAACCCAAACCGAAACCACAGCTGCTAATGTGGGCACTAAAGAAGCCTTTCCAACCA
NM_018369.2       GAAAAACCCAAACCGAAACCACAGCTGCTAATGTGGGCACTAAAGAAGCCTTTCCAACCA
                  ************************************************************

HsCD00513125      TTTCAAAGAACTAGAAGTTTTCGAATG---
NM_018369.2       TTTCAAAGAACTAGAAGTTTTCGAATGTAA
                  ***************************