DNASU Plasmid | CFHR5 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: CFHR5

Gene Name: complement factor H-related 5

Original Clone ID:

ccsbBroadEn_04233

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC111773.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513478

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1708nts

Open reading frame: 1 to 1708

Reference Sequence Alignment

ATGTTGCTCTTATTCAGTGTAATCCTAATCTCATGGGTATCCACTGTTGGGGGAGAAGGA
ACACTTTGTGATTTTCCAAAAATACACCATGGATTTCTGTATGATGAAGAAGATTATAAC
CCTTTTTCCCAAGTTCCTACAGGGGAAGTTTTCTATTACTCCTGTGAATATAATTTTGTG
TCTCCTTCAAAATCCTTTTGGACTCGCATAACATGCACAGAAGAAGGATGGTCACCAACA
CCGAAGTGTCTCAGAATGTGTTCCTTTCCTTTTGTGAAAAATGGTCATTCTGAATCTTCA
GGACTAATACATCTGGAAGGTGATACTGTACAAATTATTTGCAACACAGGATACAGCCTT
CAAAACAATGAGAAAAACATTTCGTGTGTAGAACGGGGCTGGTCCACTCCTCCCATATGC
AGCTTCACTAAAGGAGAATGTCATGTTCCAATTTTAGAAGCCAATGTAGATGCTCAGCCA
AAAAAAGAAAGCTACAAAGTTGGAGACGTGTTGAAATTCTCCTGCAGAAAAAATCTTATA
AGAGTTGGATCAGACTCAGTTCAATGTTACCAATTTGGGTGGTCACCTAACTTTCCAACA
TGCAAAGGACAAGTACGATCATGTGGTCCACCTCCTCAACTCTCCAATGGTGAAGTTAAG
GAGATAAGAAAAGAGGAATATGGACACAATGAAGTAGTGGAATATGATTGCAATCCTAAT
TTTATAATAAACGGGCCTAAGAAAATACAATGTGTGGATGGAGAATGGACAACTTTACCC
ACTTGTGTTGAACAAGTGAAAACATGTGGATACATACCTGAACTCGAGTACGGTTATGTT
CAGCCGTCTGTCCCTCCCTATCAACATGGAGTTTCAGTCGAGGTGAATTGCAGAAATGAA
TATGCAATGATTGGAAATAACATGATTACCTGTATTAATGGAATATGGACAGAGCTTCCT
ATGTGTGTTGCAACACACCAACTTAAGAGGTGCAAAATAGCAGGAGTTAATATAAAAACA
TTACTCAAGCTATCTGGGAAAGAATTTAATCATAATTCTAGAATACGTTACAGATGTTCA
GACATCTTCAGATACAGGCACTCAGTCTGTATAAACGGGAAATGGAATCCTGAAGTAGAC
TGCACAGAAAAAAGGGAACAATTCTGCCCACCGCCACCTCAGATACCTAATGCTCAGAAT
ATGACAACCACAGTGAATTATCAGGATGGAGAAAAAGTAGCTGTTCTCTGTAAAGAAAAC
TATCTACTTCCAGAAGCAAAAGAAATTGTATGTAAAGATGGACGATGGCAATCATTACCA
CGCTGTGTTGAGTCTACTGCATATTGTGGGCCCCCTCCATCTATTAACAATGGAGATACC
ACCTCATTCCCATTATCAGTATATCCTCCAGGGTCAACAGTGACGTACCGTTGCCAGTCC
TTCTATAAACTCCAGGGCTCTGTAACTGTAACATGCAGAAATAAACAGTGGTCAGAACCA
CCAAGATGCCTAGATCCATGTGTGGTATCTGAAGAAAACATGAACAAAAATAACATACAG
TTAAAATGGAGAAACGATGGAAAACTCTATGCAAAAACAGGGGATGCTGTTGAATTCCAG
TGTAAATTCCCACATAAAGCGATGATATCATCACCACCATTTCGAGCAATCTGTCAGGAA
GGGAAATTTGAATATCCTATATGTGAA

Protein sequence predicted based on nucleotide sequence

MLLLFSVILISWVSTVGGEGTLCDFPKIHHGFLYDEEDYNPFSQVPTGEVFYYSCEYNFV
SPSKSFWTRITCTEEGWSPTPKCLRMCSFPFVKNGHSESSGLIHLEGDTVQIICNTGYSL
QNNEKNISCVERGWSTPPICSFTKGECHVPILEANVDAQPKKESYKVGDVLKFSCRKNLI
RVGSDSVQCYQFGWSPNFPTCKGQVRSCGPPPQLSNGEVKEIRKEEYGHNEVVEYDCNPN
FIINGPKKIQCVDGEWTTLPTCVEQVKTCGYIPELEYGYVQPSVPPYQHGVSVEVNCRNE
YAMIGNNMITCINGIWTELPMCVATHQLKRCKIAGVNIKTLLKLSGKEFNHNSRIRYRCS
DIFRYRHSVCINGKWNPEVDCTEKREQFCPPPPQIPNAQNMTTTVNYQDGEKVAVLCKEN
YLLPEAKEIVCKDGRWQSLPRCVESTAYCGPPPSINNGDTTSFPLSVYPPGSTVTYRCQS
FYKLQGSVTVTCRNKQWSEPPRCLDPCVVSEENMNKNNIQLKWRNDGKLYAKTGDAVEFQ
CKFPHKAMISSPPFRAICQEGKFEYPICE

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

CFHR5

Symbol Nomenclature :

CFHR5

SYNONYM :

CFHL5; CFHR5D; FHR-5; FHR5

Designation :

complement factor H-related protein 5|factor H-related protein 5

Full Nomenclature :

complement factor H-related 5

GENEID :

81494

Locus Tag :

RP11-32D17.3

HGNC :

24668

MIM :

608593

Vega :

OTTHUMG00000036517

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00513478      ATGTTGCTCTTATTCAGTGTAATCCTAATCTCATGGGTATCCACTGTTGGGGGAGAAGGA
NM_030787.3       ATGTTGCTCTTATTCAGTGTAATCCTAATCTCATGGGTATCCACTGTTGGGGGAGAAGGA
                  ************************************************************

HsCD00513478      ACACTTTGTGATTTTCCAAAAATACACCATGGATTTCTGTATGATGAAGAAGATTATAAC
NM_030787.3       ACACTTTGTGATTTTCCAAAAATACACCATGGATTTCTGTATGATGAAGAAGATTATAAC
                  ************************************************************

HsCD00513478      CCTTTTTCCCAAGTTCCTACAGGGGAAGTTTTCTATTACTCCTGTGAATATAATTTTGTG
NM_030787.3       CCTTTTTCCCAAGTTCCTACAGGGGAAGTTTTCTATTACTCCTGTGAATATAATTTTGTG
                  ************************************************************

HsCD00513478      TCTCCTTCAAAATCCTTTTGGACTCGCATAACATGCACAGAAGAAGGATGGTCACCAACA
NM_030787.3       TCTCCTTCAAAATCCTTTTGGACTCGCATAACATGCACAGAAGAAGGATGGTCACCAACA
                  ************************************************************

HsCD00513478      CCGAAGTGTCTCAGAATGTGTTCCTTTCCTTTTGTGAAAAATGGTCATTCTGAATCTTCA
NM_030787.3       CCGAAGTGTCTCAGAATGTGTTCCTTTCCTTTTGTGAAAAATGGTCATTCTGAATCTTCA
                  ************************************************************

HsCD00513478      GGACTAATACATCTGGAAGGTGATACTGTACAAATTATTTGCAACACAGGATACAGCCTT
NM_030787.3       GGACTAATACATCTGGAAGGTGATACTGTACAAATTATTTGCAACACAGGATACAGCCTT
                  ************************************************************

HsCD00513478      CAAAACAATGAGAAAAACATTTCGTGTGTAGAACGGGGCTGGTCCACTCCTCCCATATGC
NM_030787.3       CAAAACAATGAGAAAAACATTTCGTGTGTAGAACGGGGCTGGTCCACTCCTCCCATATGC
                  ************************************************************

HsCD00513478      AGCTTCACTAAAGGAGAATGTCATGTTCCAATTTTAGAAGCCAATGTAGATGCTCAGCCA
NM_030787.3       AGCTTCACTAAAGGAGAATGTCATGTTCCAATTTTAGAAGCCAATGTAGATGCTCAGCCA
                  ************************************************************

HsCD00513478      AAAAAAGAAAGCTACAAAGTTGGAGACGTGTTGAAATTCTCCTGCAGAAAAAATCTTATA
NM_030787.3       AAAAAAGAAAGCTACAAAGTTGGAGACGTGTTGAAATTCTCCTGCAGAAAAAATCTTATA
                  ************************************************************

HsCD00513478      AGAGTTGGATCAGACTCAGTTCAATGTTACCAATTTGGGTGGTCACCTAACTTTCCAACA
NM_030787.3       AGAGTTGGATCAGACTCAGTTCAATGTTACCAATTTGGGTGGTCACCTAACTTTCCAACA
                  ************************************************************

HsCD00513478      TGCAAAGGACAAGTACGATCATGTGGTCCACCTCCTCAACTCTCCAATGGTGAAGTTAAG
NM_030787.3       TGCAAAGGACAAGTACGATCATGTGGTCCACCTCCTCAACTCTCCAATGGTGAAGTTAAG
                  ************************************************************

HsCD00513478      GAGATAAGAAAAGAGGAATATGGACACAATGAAGTAGTGGAATATGATTGCAATCCTAAT
NM_030787.3       GAGATAAGAAAAGAGGAATATGGACACAATGAAGTAGTGGAATATGATTGCAATCCTAAT
                  ************************************************************

HsCD00513478      TTTATAATAAACGGGCCTAAGAAAATACAATGTGTGGATGGAGAATGGACAACTTTACCC
NM_030787.3       TTTATAATAAACGGGCCTAAGAAAATACAATGTGTGGATGGAGAATGGACAACTTTACCC
                  ************************************************************

HsCD00513478      ACTTGTGTTGAACAAGTGAAAACATGTGGATACATACCTGAACTCGAGTACGGTTATGTT
NM_030787.3       ACTTGTGTTGAACAAGTGAAAACATGTGGATACATACCTGAACTCGAGTACGGTTATGTT
                  ************************************************************

HsCD00513478      CAGCCGTCTGTCCCTCCCTATCAACATGGAGTTTCAGTCGAGGTGAATTGCAGAAATGAA
NM_030787.3       CAGCCGTCTGTCCCTCCCTATCAACATGGAGTTTCAGTCGAGGTGAATTGCAGAAATGAA
                  ************************************************************

HsCD00513478      TATGCAATGATTGGAAATAACATGATTACCTGTATTAATGGAATATGGACAGAGCTTCCT
NM_030787.3       TATGCAATGATTGGAAATAACATGATTACCTGTATTAATGGAATATGGACAGAGCTTCCT
                  ************************************************************

HsCD00513478      ATGTGTGTTGCAACACACCAACTTAAGAGGTGCAAAATAGCAGGAGTTAATATAAAAACA
NM_030787.3       ATGTGTGTTGCAACACACCAACTTAAGAGGTGCAAAATAGCAGGAGTTAATATAAAAACA
                  ************************************************************

HsCD00513478      TTACTCAAGCTATCTGGGAAAGAATTTAATCATAATTCTAGAATACGTTACAGATGTTCA
NM_030787.3       TTACTCAAGCTATCTGGGAAAGAATTTAATCATAATTCTAGAATACGTTACAGATGTTCA
                  ************************************************************

HsCD00513478      GACATCTTCAGATACAGGCACTCAGTCTGTATAAACGGGAAATGGAATCCTGAAGTAGAC
NM_030787.3       GACATCTTCAGATACAGGCACTCAGTCTGTATAAACGGGAAATGGAATCCTGAAGTAGAC
                  ************************************************************

HsCD00513478      TGCACAGAAAAAAGGGAACAATTCTGCCCACCGCCACCTCAGATACCTAATGCTCAGAAT
NM_030787.3       TGCACAGAAAAAAGGGAACAATTCTGCCCACCGCCACCTCAGATACCTAATGCTCAGAAT
                  ************************************************************

HsCD00513478      ATGACAACCACAGTGAATTATCAGGATGGAGAAAAAGTAGCTGTTCTCTGTAAAGAAAAC
NM_030787.3       ATGACAACCACAGTGAATTATCAGGATGGAGAAAAAGTAGCTGTTCTCTGTAAAGAAAAC
                  ************************************************************

HsCD00513478      TATCTACTTCCAGAAGCAAAAGAAATTGTATGTAAAGATGGACGATGGCAATCATTACCA
NM_030787.3       TATCTACTTCCAGAAGCAAAAGAAATTGTATGTAAAGATGGACGATGGCAATCATTACCA
                  ************************************************************

HsCD00513478      CGCTGTGTTGAGTCTACTGCATATTGTGGGCCCCCTCCATCTATTAACAATGGAGATACC
NM_030787.3       CGCTGTGTTGAGTCTACTGCATATTGTGGGCCCCCTCCATCTATTAACAATGGAGATACC
                  ************************************************************

HsCD00513478      ACCTCATTCCCATTATCAGTATATCCTCCAGGGTCAACAGTGACGTACCGTTGCCAGTCC
NM_030787.3       ACCTCATTCCCATTATCAGTATATCCTCCAGGGTCAACAGTGACGTACCGTTGCCAGTCC
                  ************************************************************

HsCD00513478      TTCTATAAACTCCAGGGCTCTGTAACTGTAACATGCAGAAATAAACAGTGGTCAGAACCA
NM_030787.3       TTCTATAAACTCCAGGGCTCTGTAACTGTAACATGCAGAAATAAACAGTGGTCAGAACCA
                  ************************************************************

HsCD00513478      CCAAGATGCCTAGATCCATGTGTGGTATCTGAAGAAAACATGAACAAAAATAACATACAG
NM_030787.3       CCAAGATGCCTAGATCCATGTGTGGTATCTGAAGAAAACATGAACAAAAATAACATACAG
                  ************************************************************

HsCD00513478      TTAAAATGGAGAAACGATGGAAAACTCTATGCAAAAACAGGGGATGCTGTTGAATTCCAG
NM_030787.3       TTAAAATGGAGAAACGATGGAAAACTCTATGCAAAAACAGGGGATGCTGTTGAATTCCAG
                  ************************************************************

HsCD00513478      TGTAAATTCCCACATAAAGCGATGATATCATCACCACCATTTCGAGCAATCTGTCAGGAA
NM_030787.3       TGTAAATTCCCACATAAAGCGATGATATCATCACCACCATTTCGAGCAATCTGTCAGGAA
                  ************************************************************

HsCD00513478      GGGAAATTTGAATATCCTATATGTGAA---
NM_030787.3       GGGAAATTTGAATATCCTATATGTGAATGA
                  ***************************