Gene:
Gene Symbol:
SMYD4
Gene Name:
SET and MYND domain containing 4
Original Clone ID:
ccsbBroadEn_09403
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC035077.2
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00513580
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGATCTGCCTGTGGATGAGTGGAAATCATATCTGCTTCAAAAGTGGGCTTCACTCCCG
ACGTCTGTTCAGGTCACAATTTCTACAGCAGAGACCTTGAGGGATATCTTTCTTCACTCC
TCTTCACTTCTTCAACCTGAGGATGAGCTGTTTCTAAAAAGACTTTCTAAAGGTTACTTG
GTGGGAAAGGACTCGGACGCTCCTCTTTTCTACAGAGAAGAAGGAAACAAAAAATTTCAG
GAGAAAGATTACACAGGAGCTGCAGTGCTGTACTCTAAGGGAGTGTCACATTCAAGGCCT
AACACTGAGGACATGTCACTGTGTCATGCTAACCGCTCGGCAGCCCTCTTCCACCTGGGT
CAGTATGAGACGTGTCTTAAAGACATTAACATAGCACAGACACATGGGTATCCAGAAAGG
TTGCAACCCAAGATTATGTTACGTAAAGCAGAATGTCTGGTGGCCCTGGGGAGACTGCAG
GAGGCAAGCCAGACCATCAGTGATCTTGAAAGGAACTTCACAGCCACACCAGCCCTAGCA
GATGTCCTCCCTCAGACTCTGCAGAGAAACCTCCATCGTCTGAAAATGAAGATGCAAGAA
AAGGACAGTCTCACAGAAAGCTTCCCAGCAGCTCTGGCCAAAACCCTTGAGGATGCAGCG
CTGAGGGAGGAGAATGAACAACTTTCCAATGCCTCATCATCCATCGGCTTATGCGTAGAT
CCTTTAAAAGGTCGCTGTCTCGTTGCCACAAAAGATATTCTCCCAGGAGAGCTCCTGGTG
CAGGAGGATGCTTTTGTGAGTGTTCTCAACCCAGGAGAACTGCCACCACCGCATCACGGC
CTAGACAGCAAATGGGACACCAGAGTCACCAATGGGGACCTCTATTGTCACCGATGTTTG
AAGCACACTTTGGCCACAGTTCCGTGTGACGGATGCAGTTATGCCAAGTATTGCAGCCAG
GAGTGTTTGCAGCAGGTCTGGGAGCTCTACCACAGGACAGAATGTCCTCTGGGAGGGCTG
CTTCTCACACTGGGTGTCTTTTGCCACATTGCCCTGAGGTTGACTCTTTTGGTGGGATTT
GAGGATGTTCGCAAAATCATAACGAAGCTTTGTGATAAGATTAGTAACAAGGACATCTGT
TTACCTGAAAGCAACAATCAGGTCAAGACACTTAATTATGGCCTAGGGGAGAGTGAGAAA
AATGGCAACATCGTTGAGACCCCAATTCCTGGATGCGATATTAATGGGAAGTATGAAAAT
AATTATAATGCTGTCTTCAACCTTTTGCCCCACACTGAAAACCATAGCCCAGAGCACAAA
TTCCTCTGTGCTCTCTGTGTTTCTGCACTGTGCAGACAGCTAGAAGCAGCCAGTTTACAG
GCCATCCCAACTGAGAGGATTGTGAACTCCTCTCAGCTTAAAGCAGCAGTGACACCTGAA
TTGTGTCCTGACGTGACTATTTGGGGAGTGGCGATGCTGAGACACATGTTACAGCTTCAG
TGTAACGCTCAGGCGATGACCACCATACAACACACAGGACCTAAAGGGAGCATCGTTACC
GACAGCAGGCAGGTGCGCCTTGCCACAGGCATCTTCCCTGTTATCAGCCTCCTGAACCAC
TCCTGTAGCCCCAACACCAGCGTGTCCTTCATTAGCACTGTCGCCACCATCCGGGCGTCA
CAGCGGATTAGAAAGGGGCAAGAGATTCTCCACTGCTATGGGCCTCACAAGAGCCGGATG
GGGGTTGCCGAAAGGCAGCAGAAGCTGAGGTCTCAGTATTTCTTTGACTGCGCCTGTCCA
GCTTGTCAAACTGAGGCACACAGGATGGCTGCAGGGCCCAGGTGGGAAGCATTCTGTTGC
AACAGTTGCGGAGCGCCCATGCAGGGAGATGACGTGCTGCGCTGTGGCAGCAGATCTTGT
GCAGAATCCGCCGTCAGCAGGGACCACCTGGTCTCTCGGTTACAGGACCTACAGCAGCAG
GTCAGAGTGGCCCAGAAGCTTCTCAGAGATGGTGAACTAGAGCGAGCTGTTCAGCGGCTG
TCGGGGTGCCAGCGTGACGCCGAGAGCTTCCTGTGGGCAGAGCACGCCGTGGTGGGAGAG
ATCGCGGATGGCCTGGCCCGGGCCTGTGCTGCCTTAGGAGACTGGCAAAAGTCAGCCACC
CATCTACAGAGGAGTCTCTGCGTGGTGGAGGTTCGCCACGGGCCGTCCAGTGTTGAAATG
GGCCATGAGCTCTTCAAATTGGCCCAGATCTTTTTCAACGGGTTTGCAGTACCCGAAGCC
CTGAGCACAATACAGAAAGCTGAGGAGGTTCTGTCGCTGCACTGTGGCCCATGGGACGAT
GAAATCCAGGAGCTCCAGAAGATGAAATCCTGTTTGTTGGACTTACCACCCACCCCTGTA
GGGCCTGCATTG
Protein sequence predicted based on nucleotide sequence
MDLPVDEWKSYLLQKWASLPTSVQVTISTAETLRDIFLHSSSLLQPEDELFLKRLSKGYL VGKDSDAPLFYREEGNKKFQEKDYTGAAVLYSKGVSHSRPNTEDMSLCHANRSAALFHLG QYETCLKDINIAQTHGYPERLQPKIMLRKAECLVALGRLQEASQTISDLERNFTATPALA DVLPQTLQRNLHRLKMKMQEKDSLTESFPAALAKTLEDAALREENEQLSNASSSIGLCVD PLKGRCLVATKDILPGELLVQEDAFVSVLNPGELPPPHHGLDSKWDTRVTNGDLYCHRCL KHTLATVPCDGCSYAKYCSQECLQQVWELYHRTECPLGGLLLTLGVFCHIALRLTLLVGF EDVRKIITKLCDKISNKDICLPESNNQVKTLNYGLGESEKNGNIVETPIPGCDINGKYEN NYNAVFNLLPHTENHSPEHKFLCALCVSALCRQLEAASLQAIPTERIVNSSQLKAAVTPE LCPDVTIWGVAMLRHMLQLQCNAQAMTTIQHTGPKGSIVTDSRQVRLATGIFPVISLLNH SCSPNTSVSFISTVATIRASQRIRKGQEILHCYGPHKSRMGVAERQQKLRSQYFFDCACP ACQTEAHRMAAGPRWEAFCCNSCGAPMQGDDVLRCGSRSCAESAVSRDHLVSRLQDLQQQ VRVAQKLLRDGELERAVQRLSGCQRDAESFLWAEHAVVGEIADGLARACAALGDWQKSAT HLQRSLCVVEVRHGPSSVEMGHELFKLAQIFFNGFAVPEALSTIQKAEEVLSLHCGPWDD EIQELQKMKSCLLDLPPTPVGPAL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
