DNASU Plasmid | MMP19 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: MMP19

Gene Name: matrix metallopeptidase 19

Original Clone ID:

ccsbBroadEn_01024

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC050368.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513799

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1525nts

Open reading frame: 1 to 1525

Reference Sequence Alignment

ATGAACTGCCAGCAGCTGTGGCTGGGCTTCCTACTCCCCATGACAGTCTCAGGCCGGGTC
CTGGGGCTTGCAGAGGTGGCGCCCGTGGACTACCTGTCACAATATGGGTACCTACAGAAG
CCTCTAGAAGGATCTAATAACTTCAAGCCAGAAGATATCACCGAGGCTCTGAGAGCTTTT
CAGGAAGCATCTGAACTTCCAGTCTCAGGTCAGCTGGATGATGCCACAAGGGCCCGCATG
AGGCAGCCTCGTTGTGGCCTAGAGGATCCCTTCAACCAGAAGACCCTTAAATACCTGTTG
CTGGGCCGCTGGAGAAAGAAGCACCTGACTTTCCGCATCTTGAACCTGCCCTCCACCCTT
CCACCCCACACAGCCCGGGCAGCCCTGCGTCAAGCCTTCCAGGACTGGAGCAATGTGGCT
CCCTTGACCTTCCAAGAGGTGCAGGCTGGTGCGGCTGACATCCGCCTCTCCTTCCATGGC
CGCCAAAGCTCGTACTGTTCCAATACTTTTGATGGGCCTGGGAGAGTCCTGGCCCATGCC
GACATCCCAGAGCTGGGCAGTGTGCACTTCGACGAAGACGAGTTCTGGACTGAGGGGACC
TACCGTGGGGTGAACCTGCGCATCATTGCAGCCCATGAAGTGGGCCATGCTCTGGGGCTT
GGGCACTCCCGATATTCCCAGGCCCTCATGGCCCCAGTCTACGAGGGCTACCGGCCCCAC
TTTAAGCTGCACCCAGATGATGTGGCAGGGATCCAGGCTCTCTATGGCAAGAAGAGTCCA
GTGATAAGGGATGAGGAAGAAGAAGAGACAGAGCTGCCCACTGTGCCCCCAGTGCCCACA
GAACCCAGTCCCATGCCAGACCCTTGCAGTAGTGAACTGGATGCCATGATGCTGGGGCCC
CGTGGGAAGACCTATGCTTTCAAGGGGGACTATGTGTGGACTGTATCAGATTCAGGACCG
GGCCCCTTGTTCCGAGTGTCTGCCCTTTGGGAGGGGCTCCCCGGAAACCTGGATGCTGCT
GTCTACTCGCCTCGAACACAATGGATTCACTTCTTTAAGGGAGACAAGGTGTGGCGCTAC
ATTAATTTCAAGATGTCTCCTGGCTTCCCCAAGAAGCTGAATAGGGTAGAACCTAACCTG
GATGCAGCTCTCTATTGGCCTCTCAACCAAAAGGTGTTCCTCTTTAAGGGCTCCGGGTAC
TGGCAGTGGGACGAGCTAGCCCGAACTGACTTCAGCAGCTACCCCAAACCAATCAAGGGT
TTGTTTACGGGAGTGCCAAACCAGCCCTCGGCTGCTATGAGTTGGCAAGATGGCCGAGTC
TACTTCTTCAAGGGCAAAGTCTACTGGCGCCTCAACCAGCAGCTTCGAGTAGAGAAAGGC
TATCCCAGAAATATTTCCCACAACTGGATGCACTGTCGTCCCCGGACTATAGACACTACC
CCATCAGGTGGGAATACCACTCCCTCAGGTACGGGCATAACCTTGGATACCACTCTCTCA
GCCACAGAAACCACGTTTGAATAC

Protein sequence predicted based on nucleotide sequence

MNCQQLWLGFLLPMTVSGRVLGLAEVAPVDYLSQYGYLQKPLEGSNNFKPEDITEALRAF
QEASELPVSGQLDDATRARMRQPRCGLEDPFNQKTLKYLLLGRWRKKHLTFRILNLPSTL
PPHTARAALRQAFQDWSNVAPLTFQEVQAGAADIRLSFHGRQSSYCSNTFDGPGRVLAHA
DIPELGSVHFDEDEFWTEGTYRGVNLRIIAAHEVGHALGLGHSRYSQALMAPVYEGYRPH
FKLHPDDVAGIQALYGKKSPVIRDEEEEETELPTVPPVPTEPSPMPDPCSSELDAMMLGP
RGKTYAFKGDYVWTVSDSGPGPLFRVSALWEGLPGNLDAAVYSPRTQWIHFFKGDKVWRY
INFKMSPGFPKKLNRVEPNLDAALYWPLNQKVFLFKGSGYWQWDELARTDFSSYPKPIKG
LFTGVPNQPSAAMSWQDGRVYFFKGKVYWRLNQQLRVEKGYPRNISHNWMHCRPRTIDTT
PSGGNTTPSGTGITLDTTLSATETTFEY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

MMP19

Symbol Nomenclature :

MMP19

SYNONYM :

MMP18; RASI-1

Designation :

matrix metalloproteinase RASI|matrix metalloproteinase-18|matrix metalloproteinase-19|matrix metalloproteinase-beta19

Full Nomenclature :

matrix metallopeptidase 19

GENEID :

4327

GenBank Accession :

HQ448618

HGNC :

7165

MIM :

601807

Target GenBank:

HQ448618

Reference Sequence Alignment

  

HsCD00513799      ATGAACTGCCAGCAGCTGTGGCTGGGCTTCCTACTCCCCATGACAGTCTCAGGCCGGGTC
NM_002429.5       ATGAACTGCCAGCAGCTGTGGCTGGGCTTCCTACTCCCCATGACAGTCTCAGGCCGGGTC
                  ************************************************************

HsCD00513799      CTGGGGCTTGCAGAGGTGGCGCCCGTGGACTACCTGTCACAATATGGGTACCTACAGAAG
NM_002429.5       CTGGGGCTTGCAGAGGTGGCGCCCGTGGACTACCTGTCACAATATGGGTACCTACAGAAG
                  ************************************************************

HsCD00513799      CCTCTAGAAGGATCTAATAACTTCAAGCCAGAAGATATCACCGAGGCTCTGAGAGCTTTT
NM_002429.5       CCTCTAGAAGGATCTAATAACTTCAAGCCAGAAGATATCACCGAGGCTCTGAGAGCTTTT
                  ************************************************************

HsCD00513799      CAGGAAGCATCTGAACTTCCAGTCTCAGGTCAGCTGGATGATGCCACAAGGGCCCGCATG
NM_002429.5       CAGGAAGCATCTGAACTTCCAGTCTCAGGTCAGCTGGATGATGCCACAAGGGCCCGCATG
                  ************************************************************

HsCD00513799      AGGCAGCCTCGTTGTGGCCTAGAGGATCCCTTCAACCAGAAGACCCTTAAATACCTGTTG
NM_002429.5       AGGCAGCCTCGTTGTGGCCTAGAGGATCCCTTCAACCAGAAGACCCTTAAATACCTGTTG
                  ************************************************************

HsCD00513799      CTGGGCCGCTGGAGAAAGAAGCACCTGACTTTCCGCATCTTGAACCTGCCCTCCACCCTT
NM_002429.5       CTGGGCCGCTGGAGAAAGAAGCACCTGACTTTCCGCATCTTGAACCTGCCCTCCACCCTT
                  ************************************************************

HsCD00513799      CCACCCCACACAGCCCGGGCAGCCCTGCGTCAAGCCTTCCAGGACTGGAGCAATGTGGCT
NM_002429.5       CCACCCCACACAGCCCGGGCAGCCCTGCGTCAAGCCTTCCAGGACTGGAGCAATGTGGCT
                  ************************************************************

HsCD00513799      CCCTTGACCTTCCAAGAGGTGCAGGCTGGTGCGGCTGACATCCGCCTCTCCTTCCATGGC
NM_002429.5       CCCTTGACCTTCCAAGAGGTGCAGGCTGGTGCGGCTGACATCCGCCTCTCCTTCCATGGC
                  ************************************************************

HsCD00513799      CGCCAAAGCTCGTACTGTTCCAATACTTTTGATGGGCCTGGGAGAGTCCTGGCCCATGCC
NM_002429.5       CGCCAAAGCTCGTACTGTTCCAATACTTTTGATGGGCCTGGGAGAGTCCTGGCCCATGCC
                  ************************************************************

HsCD00513799      GACATCCCAGAGCTGGGCAGTGTGCACTTCGACGAAGACGAGTTCTGGACTGAGGGGACC
NM_002429.5       GACATCCCAGAGCTGGGCAGTGTGCACTTCGACGAAGACGAGTTCTGGACTGAGGGGACC
                  ************************************************************

HsCD00513799      TACCGTGGGGTGAACCTGCGCATCATTGCAGCCCATGAAGTGGGCCATGCTCTGGGGCTT
NM_002429.5       TACCGTGGGGTGAACCTGCGCATCATTGCAGCCCATGAAGTGGGCCATGCTCTGGGGCTT
                  ************************************************************

HsCD00513799      GGGCACTCCCGATATTCCCAGGCCCTCATGGCCCCAGTCTACGAGGGCTACCGGCCCCAC
NM_002429.5       GGGCACTCCCGATATTCCCAGGCCCTCATGGCCCCAGTCTACGAGGGCTACCGGCCCCAC
                  ************************************************************

HsCD00513799      TTTAAGCTGCACCCAGATGATGTGGCAGGGATCCAGGCTCTCTATGGCAAGAAGAGTCCA
NM_002429.5       TTTAAGCTGCACCCAGATGATGTGGCAGGGATCCAGGCTCTCTATGGCAAGAAGAGTCCA
                  ************************************************************

HsCD00513799      GTGATAAGGGATGAGGAAGAAGAAGAGACAGAGCTGCCCACTGTGCCCCCAGTGCCCACA
NM_002429.5       GTGATAAGGGATGAGGAAGAAGAAGAGACAGAGCTGCCCACTGTGCCCCCAGTGCCCACA
                  ************************************************************

HsCD00513799      GAACCCAGTCCCATGCCAGACCCTTGCAGTAGTGAACTGGATGCCATGATGCTGGGGCCC
NM_002429.5       GAACCCAGTCCCATGCCAGACCCTTGCAGTAGTGAACTGGATGCCATGATGCTGGGGCCC
                  ************************************************************

HsCD00513799      CGTGGGAAGACCTATGCTTTCAAGGGGGACTATGTGTGGACTGTATCAGATTCAGGACCG
NM_002429.5       CGTGGGAAGACCTATGCTTTCAAGGGGGACTATGTGTGGACTGTATCAGATTCAGGACCG
                  ************************************************************

HsCD00513799      GGCCCCTTGTTCCGAGTGTCTGCCCTTTGGGAGGGGCTCCCCGGAAACCTGGATGCTGCT
NM_002429.5       GGCCCCTTGTTCCGAGTGTCTGCCCTTTGGGAGGGGCTCCCCGGAAACCTGGATGCTGCT
                  ************************************************************

HsCD00513799      GTCTACTCGCCTCGAACACAATGGATTCACTTCTTTAAGGGAGACAAGGTGTGGCGCTAC
NM_002429.5       GTCTACTCGCCTCGAACACAATGGATTCACTTCTTTAAGGGAGACAAGGTGTGGCGCTAC
                  ************************************************************

HsCD00513799      ATTAATTTCAAGATGTCTCCTGGCTTCCCCAAGAAGCTGAATAGGGTAGAACCTAACCTG
NM_002429.5       ATTAATTTCAAGATGTCTCCTGGCTTCCCCAAGAAGCTGAATAGGGTAGAACCTAACCTG
                  ************************************************************

HsCD00513799      GATGCAGCTCTCTATTGGCCTCTCAACCAAAAGGTGTTCCTCTTTAAGGGCTCCGGGTAC
NM_002429.5       GATGCAGCTCTCTATTGGCCTCTCAACCAAAAGGTGTTCCTCTTTAAGGGCTCCGGGTAC
                  ************************************************************

HsCD00513799      TGGCAGTGGGACGAGCTAGCCCGAACTGACTTCAGCAGCTACCCCAAACCAATCAAGGGT
NM_002429.5       TGGCAGTGGGACGAGCTAGCCCGAACTGACTTCAGCAGCTACCCCAAACCAATCAAGGGT
                  ************************************************************

HsCD00513799      TTGTTTACGGGAGTGCCAAACCAGCCCTCGGCTGCTATGAGTTGGCAAGATGGCCGAGTC
NM_002429.5       TTGTTTACGGGAGTGCCAAACCAGCCCTCGGCTGCTATGAGTTGGCAAGATGGCCGAGTC
                  ************************************************************

HsCD00513799      TACTTCTTCAAGGGCAAAGTCTACTGGCGCCTCAACCAGCAGCTTCGAGTAGAGAAAGGC
NM_002429.5       TACTTCTTCAAGGGCAAAGTCTACTGGCGCCTCAACCAGCAGCTTCGAGTAGAGAAAGGC
                  ************************************************************

HsCD00513799      TATCCCAGAAATATTTCCCACAACTGGATGCACTGTCGTCCCCGGACTATAGACACTACC
NM_002429.5       TATCCCAGAAATATTTCCCACAACTGGATGCACTGTCGTCCCCGGACTATAGACACTACC
                  ************************************************************

HsCD00513799      CCATCAGGTGGGAATACCACTCCCTCAGGTACGGGCATAACCTTGGATACCACTCTCTCA
NM_002429.5       CCATCAGGTGGGAATACCACTCCCTCAGGTACGGGCATAACCTTGGATACCACTCTCTCA
                  ************************************************************

HsCD00513799      GCCACAGAAACCACGTTTGAATAC---
NM_002429.5       GCCACAGAAACCACGTTTGAATACTGA
                  ************************

Pathways

Panther :

Alzheimer disease-presenilin pathway

Reactome :

Collagen degradation

Reactome :

Degradation of the extracellular matrix

Reactome :

Extracellular matrix organization

WikiPathway :

Matrix Metalloproteinases

Protein

SMART domain :

ZnMc : Zinc-dependent metalloprotease

SMART domain :

HX : Hemopexin-like repeats.

HPRD :

03486

Clone

Cloning Information :

606309

Original Clone ID :

ccsbBroadEn_01024

Organism

Species Specific ID:

4327

Genome

Chromosome :

Map Location :

12q14

Reagents

Labome :

MMP19-antibody