DNASU Plasmid | C1orf124 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: SPRTN

Gene Name: SprT-like N-terminal domain

Original Clone ID:

ccsbBroadEn_09134

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC068478.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513836

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1468nts

Open reading frame: 1 to 1468

Reference Sequence Alignment

ATGGATGATGACTTGATGTTGGCACTGCGGCTTCAGGAGGAGTGGAACTTGCAGGAGGCG
GAGCGCGATCATGCCCAGGAGTCCCTGTCGCTAGTGGACGCGTCGTGGGAGTTGGTGGAC
CCCACACCGGACTTGCAGGCACTGTTTGTTCAGTTTAACGACCAATTCTTCTGGGGCCAG
CTGGAGGCCGTCGAGGTGAAGTGGAGCGTGCGAATGACCCTGTGTGCTGGGATATGCAGC
TATGAAGGGAAGGGTGGAATGTGTTCCATCCGTCTCAGCGAACCCCTTTTGAAGTTGAGG
CCAAGAAAGGATCTTGTAGAGACCCTCCTGCATGAAATGATACATGCCTATTTATTTGTC
ACTAATAACGACAAAGACCGAGAAGGGCATGGTCCAGAATTTTGTAAACATATGCATCGC
ATCAACAGCCTGACTGGAGCCAATATAACGGTATACCATACTTTTCACGATGAGGTGGAT
GAGTATCGGCGACACTGGTGGCGCTGCAATGGGCCGTGCCAGCACAGGCCACCGTATTAC
GGCTATGTCAAACGAGCTACTAACAGGGAACCCTCTGCTCATGACTATTGGTGGGCTGAG
CACCAGAAAACCTGTGGAGGCACTTACATAAAAATCAAGGAACCAGAGAATTACTCAAAA
AAAGGCAAAGGAAAGGCAAAACTAGGAAAGGAACCAGTATTGGCCGCAGAGAATAAAGAT
AAACCCAACAGAGGTGAGGCCCAGCTAGTAATCCCTTTTAGTGGGAAAGGATATGTTCTA
GGAGAAACAAGCAATTTACCTTCACCTGGGAAACTGATCACTTCACATGCCATTAATAAA
ACCCAAGATCTTTTAAATCAAAACCATTCAGCAAATGCTGTAAGACTTAATTCTAAAATC
AAGGTGAAATTTGAACAGAATGGTTCAAGTAAAAATTCTCATCTGGTCTCCCCTGCTGTT
AGTAACAGTCACCAAAATGTTCTAAGCAACTACTTTCCTAGAGTATCATTTGCCAACCAA
AAGGCTTTCAGAGGTGTGAATGGATCTCCAAGGATAAGTGTAACAGTTGGCAACATCCCT
AAAAACTCAGTCTCTTCTAGTTCTCAGAGAAGGGTTTCATCTTCTAAGATATCCCTAAGA
AATTCTTCAAAAGTAACGGAATCAGCATCTGTGATGCCATCCCAGGATGTGAGTGGGTCT
GAAGATACATTCCCAAATAAACGACCTAGGCTAGAAGATAAGACTGTTTTTGACAATTTT
TTTATCAAGAAAGAGCAAATAAAAAGCAGTGGTAATGATCCAAAGTATAGTACAACCACA
GCTCAGAATTCCAGCAGTTCATCCAGTCAGAGCAAAATGGTTAATTGCCCAGTTTGTCAG
AATGAAGTTCTGGAGTCTCAGATTAATGAGCACTTGGACTGGTGCCTTGAAGGTGACAGC
ATCAAAGTCAAAAGCGAAGAAAGTCTT

Protein sequence predicted based on nucleotide sequence

MDDDLMLALRLQEEWNLQEAERDHAQESLSLVDASWELVDPTPDLQALFVQFNDQFFWGQ
LEAVEVKWSVRMTLCAGICSYEGKGGMCSIRLSEPLLKLRPRKDLVETLLHEMIHAYLFV
TNNDKDREGHGPEFCKHMHRINSLTGANITVYHTFHDEVDEYRRHWWRCNGPCQHRPPYY
GYVKRATNREPSAHDYWWAEHQKTCGGTYIKIKEPENYSKKGKGKAKLGKEPVLAAENKD
KPNRGEAQLVIPFSGKGYVLGETSNLPSPGKLITSHAINKTQDLLNQNHSANAVRLNSKI
KVKFEQNGSSKNSHLVSPAVSNSHQNVLSNYFPRVSFANQKAFRGVNGSPRISVTVGNIP
KNSVSSSSQRRVSSSKISLRNSSKVTESASVMPSQDVSGSEDTFPNKRPRLEDKTVFDNF
FIKKEQIKSSGNDPKYSTTTAQNSSSSSSQSKMVNCPVCQNEVLESQINEHLDWCLEGDS
IKVKSEESL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

SPRTN

Symbol Nomenclature :

SPRTN

SYNONYM :

C1orf124; DDDL1880; DVC1; PRO4323; Spartan; dJ876B10.3

Designation :

DNA damage protein targeting VCP|DNA damage-targeting VCP (p97) adaptor|SprT-like domain at the N terminus|protein with SprT-like domain at the N terminus|sprT-like domain-containing protein Spartan|zinc finger RAD18 domain-containing protein C1orf124

Full Nomenclature :

SprT-like N-terminal domain

GENEID :

83932

Locus Tag :

RP5-876B10.3

GenBank Accession :

AM393630

HGNC :

25356

Vega :

OTTHUMG00000038022

Target GenBank:

AM393630

Reference Sequence Alignment

  

HsCD00513836      ATGGATGATGACTTGATGTTGGCACTGCGGCTTCAGGAGGAGTGGAACTTGCAGGAGGCG
NM_032018.5       ATGGATGATGACTTGATGTTGGCACTGCGGCTTCAGGAGGAGTGGAACTTGCAGGAGGCG
                  ************************************************************

HsCD00513836      GAGCGCGATCATGCCCAGGAGTCCCTGTCGCTAGTGGACGCGTCGTGGGAGTTGGTGGAC
NM_032018.5       GAGCGCGATCATGCCCAGGAGTCCCTGTCGCTAGTGGACGCGTCGTGGGAGTTGGTGGAC
                  ************************************************************

HsCD00513836      CCCACACCGGACTTGCAGGCACTGTTTGTTCAGTTTAACGACCAATTCTTCTGGGGCCAG
NM_032018.5       CCCACACCGGACTTGCAGGCACTGTTTGTTCAGTTTAACGACCAATTCTTCTGGGGCCAG
                  ************************************************************

HsCD00513836      CTGGAGGCCGTCGAGGTGAAGTGGAGCGTGCGAATGACCCTGTGTGCTGGGATATGCAGC
NM_032018.5       CTGGAGGCCGTCGAGGTGAAGTGGAGCGTGCGAATGACCCTGTGTGCTGGGATATGCAGC
                  ************************************************************

HsCD00513836      TATGAAGGGAAGGGTGGAATGTGTTCCATCCGTCTCAGCGAACCCCTTTTGAAGTTGAGG
NM_032018.5       TATGAAGGGAAGGGTGGAATGTGTTCCATCCGTCTCAGCGAACCCCTTTTGAAGTTGAGG
                  ************************************************************

HsCD00513836      CCAAGAAAGGATCTTGTAGAGACCCTCCTGCATGAAATGATACATGCCTATTTATTTGTC
NM_032018.5       CCAAGAAAGGATCTTGTAGAGACCCTCCTGCATGAAATGATACATGCCTATTTATTTGTC
                  ************************************************************

HsCD00513836      ACTAATAACGACAAAGACCGAGAAGGGCATGGTCCAGAATTTTGTAAACATATGCATCGC
NM_032018.5       ACTAATAACGACAAAGACCGAGAAGGGCATGGTCCAGAATTTTGTAAACATATGCATCGC
                  ************************************************************

HsCD00513836      ATCAACAGCCTGACTGGAGCCAATATAACGGTATACCATACTTTTCACGATGAGGTGGAT
NM_032018.5       ATCAACAGCCTGACTGGAGCCAATATAACGGTATACCATACTTTTCACGATGAGGTGGAT
                  ************************************************************

HsCD00513836      GAGTATCGGCGACACTGGTGGCGCTGCAATGGGCCGTGCCAGCACAGGCCACCGTATTAC
NM_032018.5       GAGTATCGGCGACACTGGTGGCGCTGCAATGGGCCGTGCCAGCACAGGCCACCGTATTAC
                  ************************************************************

HsCD00513836      GGCTATGTCAAACGAGCTACTAACAGGGAACCCTCTGCTCATGACTATTGGTGGGCTGAG
NM_032018.5       GGCTATGTCAAACGAGCTACTAACAGGGAACCCTCTGCTCATGACTATTGGTGGGCTGAG
                  ************************************************************

HsCD00513836      CACCAGAAAACCTGTGGAGGCACTTACATAAAAATCAAGGAACCAGAGAATTACTCAAAA
NM_032018.5       CACCAGAAAACCTGTGGAGGCACTTACATAAAAATCAAGGAACCAGAGAATTACTCAAAA
                  ************************************************************

HsCD00513836      AAAGGCAAAGGAAAGGCAAAACTAGGAAAGGAACCAGTATTGGCCGCAGAGAATAAAGAT
NM_032018.5       AAAGGCAAAGGAAAGGCAAAACTAGGAAAGGAACCAGTATTGGCCGCAGAGAATAAAGAT
                  ************************************************************

HsCD00513836      AAACCCAACAGAGGTGAGGCCCAGCTAGTAATCCCTTTTAGTGGGAAAGGATATGTTCTA
NM_032018.5       AAACCCAACAGAGGTGAGGCCCAGCTAGTAATCCCTTTTAGTGGGAAAGGATATGTTCTA
                  ************************************************************

HsCD00513836      GGAGAAACAAGCAATTTACCTTCACCTGGGAAACTGATCACTTCACATGCCATTAATAAA
NM_032018.5       GGAGAAACAAGCAATTTACCTTCACCTGGGAAACTGATCACTTCACATGCCATTAATAAA
                  ************************************************************

HsCD00513836      ACCCAAGATCTTTTAAATCAAAACCATTCAGCAAATGCTGTAAGACTTAATTCTAAAATC
NM_032018.5       ACCCAAGATCTTTTAAATCAAAACCATTCAGCAAATGCTGTAAGACCTAATTCTAAAATC
                  ********************************************** *************

HsCD00513836      AAGGTGAAATTTGAACAGAATGGTTCAAGTAAAAATTCTCATCTGGTCTCCCCTGCTGTT
NM_032018.5       AAGGTGAAATTTGAACAGAATGGTTCAAGTAAAAATTCTCATCTGGTCTCCCCTGCTGTT
                  ************************************************************

HsCD00513836      AGTAACAGTCACCAAAATGTTCTAAGCAACTACTTTCCTAGAGTATCATTTGCCAACCAA
NM_032018.5       AGTAACAGTCACCAAAATGTTCTAAGCAACTACTTTCCTAGAGTATCATTTGCCAACCAA
                  ************************************************************

HsCD00513836      AAGGCTTTCAGAGGTGTGAATGGATCTCCAAGGATAAGTGTAACAGTTGGCAACATCCCT
NM_032018.5       AAGGCTTTCAGAGGTGTGAATGGATCTCCAAGGATAAGTGTAACAGTTGGCAACATCCCT
                  ************************************************************

HsCD00513836      AAAAACTCAGTCTCTTCTAGTTCTCAGAGAAGGGTTTCATCTTCTAAGATATCCCTAAGA
NM_032018.5       AAAAACTCAGTCTCTTCTAGTTCTCAGAGAAGGGTTTCATCTTCTAAGATATCCCTAAGA
                  ************************************************************

HsCD00513836      AATTCTTCAAAAGTAACGGAATCAGCATCTGTGATGCCATCCCAGGATGTGAGTGGGTCT
NM_032018.5       AATTCTTCAAAAGTAACGGAATCAGCATCTGTGATGCCATCCCAGGATGTGAGTGGGTCT
                  ************************************************************

HsCD00513836      GAAGATACATTCCCAAATAAACGACCTAGGCTAGAAGATAAGACTGTTTTTGACAATTTT
NM_032018.5       GAAGATACATTCCCAAATAAACGACCTAGGCTAGAAGATAAGACTGTTTTTGACAATTTT
                  ************************************************************

HsCD00513836      TTTATCAAGAAAGAGCAAATAAAAAGCAGTGGTAATGATCCAAAGTATAGTACAACCACA
NM_032018.5       TTTATCAAGAAAGAGCAAATAAAAAGCAGTGGTAATGATCCAAAGTATAGTACAACCACA
                  ************************************************************

HsCD00513836      GCTCAGAATTCCAGCAGTTCATCCAGTCAGAGCAAAATGGTTAATTGCCCAGTTTGTCAG
NM_032018.5       GCTCAGAATTCCAGCAGTTCATCCAGTCAGAGCAAAATGGTTAATTGCCCAGTTTGTCAG
                  ************************************************************

HsCD00513836      AATGAAGTTCTGGAGTCTCAGATTAATGAGCACTTGGACTGGTGCCTTGAAGGTGACAGC
NM_032018.5       AATGAAGTTCTGGAGTCTCAGATTAATGAGCACTTGGACTGGTGCCTTGAAGGTGACAGC
                  ************************************************************

HsCD00513836      ATCAAAGTCAAAAGCGAAGAAAGTCTT---
NM_032018.5       ATCAAAGTCAAAAGCGAAGAAAGTCTTTGA
                  ***************************