PRPF4 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  PRPF4
Gene Name:  pre-mRNA processing factor 4
Original Clone ID:
ccsbBroadEn_11339
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC007424.2
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00513891
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1564nts
Open reading frame: 1 to 1564

ATGGCTTCCTCGCGAGCCTCTTCCACGGCAACCAAAACTAAAGCACCCGACGACTTAGTT
GCTCCGGTCGTGAAGAAACCACACATCTATTATGGAAGTTTGGAAGAGAAGGAGAGGGAG
CGTCTGGCCAAAGGAGAGTCTGGGATTTTGGGGAAAGACGGACTTAAAGCAGGGATCGAA
GCTGGAAATATTAATATAACCTCTGGAGAAGTGTTTGAAATTGAAGAGCATATCAGCGAG
CGACAGGCAGAAGTATTGGCTGAGTTTGAGAGAAGGAAGCGAGCCCGGCAGATCAATGTT
TCCACAGATGACTCAGAGGTCAAAGCTTGCCTTAGAGCCTTGGGGGAACCCATCACACTT
TTTGGAGAGGGTCCTGCTGAAAGAAGAGAAAGGTTAAGAAATATCCTCTCAGTTGTCGGT
ACTGATGCCTTGAAAAAGACCAAAAAGGATGATGAGAAGTCTAAAAAGTCCAAAGAAGAG
TATCAGCAAACCTGGTATCATGAAGGACCAAATAGCTTGAAGGTGGCAAGACTATGGATT
GCTAATTATTCGTTGCCCAGGGCAATGAAACGCTTGGAAGAGGCCCGACTCCATAAGGAG
ATTCCTGAGACAACAAGGACCTCCCAGATGCAAGAGCTGCACAAGTCTCTCCGGTCTTTG
AATAATTTTTGCAGTCAGATTGGGGATGATCGGCCTATCTCCTACTGTCACTTTAGTCCC
AATTCCAAGATGCTGGCCACAGCTTGTTGGAGTGGGCTTTGCAAGCTCTGGTCTGTTCCT
GATTGCAACCTCCTTCACACTCTTCGAGGGCATAACACAAATGTAGGAGCAATTGTATTC
CATCCCAAATCCACTGTCTCCTTGGACCCAAAAGATGTCAACCTGGCCTCTTGTGCGGCT
GATGGCTCTGTGAAGCTTTGGAGTCTCGACAGTGATGAACCAGTGGCAGATATTGAAGGC
CATACAGTGCGTGTGGCGCGGGTAATGTGGCATCCTTCAGGACGTTTCCTGGGCACCACC
TGCTATGACCGTTCATGGCGCTTATGGGATTTGGAGGCTCAAGAGGAGATCCTGCATCAG
GAAGGCCATAGCATGGGTGTGTATGACATTGCCTTCCATCAAGATGGCTCTTTGGCTGGC
ACTGGGGGACTGGATGCATTTGGTCGAGTTTGGGACCTACGCACAGGACGTTGTATCATG
TTCTTAGAAGGCCACCTGAAAGAAATCTATGGAATAAATTTCTCCCCCAATGGCTATCAC
ATTGCAACCGGCAGTGGTGACAACACCTGCAAAGTGTGGGACCTCCGACAGCGGCGTTGC
GTCTACACCATCCCTGCTCATCAGAACTTAGTGACTGGTGTCAAGTTTGAGCCTATCCAT
GGGAACTTCTTGCTTACTGGTGCCTATGATAACACAGCCAAGATCTGGACGCACCCAGGC
TGGTCCCCGCTGAAGACTCTGGCTGGCCACGAAGGCAAAGTGATGGGCCTAGATATTTCT
TCCGATGGGCAGCTCATAGCCACTTGCTCATATGACAGGACCTTCAAGCTGTGGATGGCT
GAA

Protein sequence predicted based on nucleotide sequence

MASSRASSTATKTKAPDDLVAPVVKKPHIYYGSLEEKERERLAKGESGILGKDGLKAGIE
AGNINITSGEVFEIEEHISERQAEVLAEFERRKRARQINVSTDDSEVKACLRALGEPITL
FGEGPAERRERLRNILSVVGTDALKKTKKDDEKSKKSKEEYQQTWYHEGPNSLKVARLWI
ANYSLPRAMKRLEEARLHKEIPETTRTSQMQELHKSLRSLNNFCSQIGDDRPISYCHFSP
NSKMLATACWSGLCKLWSVPDCNLLHTLRGHNTNVGAIVFHPKSTVSLDPKDVNLASCAA
DGSVKLWSLDSDEPVADIEGHTVRVARVMWHPSGRFLGTTCYDRSWRLWDLEAQEEILHQ
EGHSMGVYDIAFHQDGSLAGTGGLDAFGRVWDLRTGRCIMFLEGHLKEIYGINFSPNGYH
IATGSGDNTCKVWDLRQRRCVYTIPAHQNLVTGVKFEPIHGNFLLTGAYDNTAKIWTHPG
WSPLKTLAGHEGKVMGLDISSDGQLIATCSYDRTFKLWMAE

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable