DNASU Plasmid | FRMD3 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: FRMD3

Gene Name: FERM domain containing 3

Original Clone ID:

ccsbBroadEn_13478

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC041376.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513973

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1744nts

Open reading frame: 1 to 1744

Reference Sequence Alignment

ATGATCCACTTTCGGAGCTCCAGCGTCAAATCGCTCAGCCAGGAGATGAGATGCACCATC
CGGCTGCTGGACGACTCGGAGATCTCCTGCCACATCCAGAGGGAAACCAAAGGGCAGTTT
CTCATTGACCACATCTGCAACTACTACAGCCTGCTGGAGAAGGACTACTTTGGCATTCGC
TATGTGGACCCAGAGAAGCAAAGGCACTGGCTTGAACCTAACAAGTCCATCTTCAAGCAA
ATGAAAACTCATCCACCATACACCATGTGCTTTAGAGTGAAATTCTACCCACATGAACCC
TTGAAGATTAAAGAAGAGCTCACAAGATACCTTTTATACCTTCAGATTAAAAGGGACATT
TTTCATGGCCACCTGCTGTGCTCCTTTTCTGATGCTGCCTACCTGGGTGCCTGTATTGTT
CAAGCTGAGCTTGGTGATTACGATCCTAATGAGCATCCTGAGAATTACATCAGTGAGTTT
GAGATTTTCCCCAAGCAGTCACAGAAGCTGGAAAGAAAAATAGTGGAAATTCATAAAAAT
GAACTCAGGGGGCAGAGCCCACCAGTTGCTGAATTTAACTTGCTCCTGAAAGCTCACACT
TTGGAAACCTACGGGGTGGATCCTCACCCATGCAAGGATTCAACAGGCACAACAACATTT
TTAGGATTCACAGCTGCAGGCTTTGTGGTCTTTCAGGGAAATAAGAGAATCCATTTGATA
AAATGGCCAGATGTCTGCAAATTGAAGTTTGAAGGGAAGACATTTTATGTGATTGGCACC
CAGAAGGAGAAAAAAGCCATGTTGGCATTCAATACTTCAACACCAGCTGCCTGCAAACAT
CTTTGGAAGTGTGGAGTGGAAAACCAGGCCTTTTATAAGTATGCAAAATCCAGTCAGATC
AAGACTGTATCAAGCAGCAAGATATTTTTTAAAGGAAGTAGATTTCGATATAGTGGGAAA
GTTGCCAAAGAGGTGGTGGAGGCCAGTTCCAAGATCCAGAGGGAGCCTCCTGAGGTGCAC
AGAGCCAACATTACTCAGAGCCGCAGTTCCCACTCCTTGAACAAACAGCTCATCATTAAC
ATGGAACCCCTGCAGCCCCTGCTTCCTTCCCCCAGCGAGCAAGAAGAAGAACTTCCTCTG
GGTGAGGGTGTTCCATTGCCTAAAGAGGAGAACATTTCTGCTCCCTTGATCTCCAGCTCC
CCAGTGAAGGCAGCCCGGGAGTATGAAGATCCCCCTAGTGAAGAGGAAGATAAAATAAAA
GAAGAACCTTTAACCATCTCTGAACTAGTGTACAACCCAAGTGCCAGCCTGCTCCCCACC
CCTGTGGATGACGATGAGATTGACATGCTCTTTGACTGTCCTTCTAGGCTTGAGTTGGAA
AGAGAAGACACAGATTCATTTGAGGATCTGGAAGCAGATGAAAACGCCTTTTTGATTGCT
GAAGAAGAGGAGCTGAAGGAGGCTCGCCGTGCTTTGTCGTGGAGCTACGACATTCTGACT
GGCCATATTCGGGTGAACCCACTGGTCAAGAGTTTTTCCAGGCTCCTTGTGGTGGGCCTG
GGACTGCTGCTCTTTGTATTTCCCCTGCTCCTCCTCCTTTTGGAGTCAGGTATTGATCTC
TCCTTCTTATGCGAAATCCGCCAGACACCAGAGTTTGAGCAGTTTCACTATGAATACTAC
TGTCCCCTCAAGGAGTGGGTGGCTGGGAAAGTCCACCTCATCCTCTACATGCTGGGTTGC
TCA

Protein sequence predicted based on nucleotide sequence

MIHFRSSSVKSLSQEMRCTIRLLDDSEISCHIQRETKGQFLIDHICNYYSLLEKDYFGIR
YVDPEKQRHWLEPNKSIFKQMKTHPPYTMCFRVKFYPHEPLKIKEELTRYLLYLQIKRDI
FHGHLLCSFSDAAYLGACIVQAELGDYDPNEHPENYISEFEIFPKQSQKLERKIVEIHKN
ELRGQSPPVAEFNLLLKAHTLETYGVDPHPCKDSTGTTTFLGFTAAGFVVFQGNKRIHLI
KWPDVCKLKFEGKTFYVIGTQKEKKAMLAFNTSTPAACKHLWKCGVENQAFYKYAKSSQI
KTVSSSKIFFKGSRFRYSGKVAKEVVEASSKIQREPPEVHRANITQSRSSHSLNKQLIIN
MEPLQPLLPSPSEQEEELPLGEGVPLPKEENISAPLISSSPVKAAREYEDPPSEEEDKIK
EEPLTISELVYNPSASLLPTPVDDDEIDMLFDCPSRLELEREDTDSFEDLEADENAFLIA
EEEELKEARRALSWSYDILTGHIRVNPLVKSFSRLLVVGLGLLLFVFPLLLLLLESGIDL
SFLCEIRQTPEFEQFHYEYYCPLKEWVAGKVHLILYMLGCS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

FRMD3

Symbol Nomenclature :

FRMD3

SYNONYM :

4.1O; EPB41L4O; EPB41LO; P410

Designation :

FERM domain-containing protein 3|band 4.1-like protein 4|band 4.1-like protein 4O|ovary type protein 4.1|protein 4.1O

Full Nomenclature :

FERM domain containing 3

GENEID :

257019

Locus Tag :

RP11-439K3.2

GenBank Accession :

GQ129452

HGNC :

24125

MIM :

607619

Vega :

OTTHUMG00000020103

Target GenBank:

GQ129452

Reference Sequence Alignment

  

HsCD00513973        ------------------------------------------------ATGATCCACTTT
NM_174938.5         ATGTTCGCCTCCTGCCACTGTGTGCCGAGAGGCAGGAGGACCATGAAAATGATCCACTTT
NM_001244960.1      ------------------------------------------------------------
                                                                                

HsCD00513973        CGGAGCTCCAGCGTCAAATCGCTCAGCCAGGAGATGAGATGCACCATCCGGCTGCTGGAC
NM_174938.5         CGGAGCTCCAGCGTCAAATCGCTCAGCCAGGAGATGAGATGCACCATCCGGCTGCTGGAC
NM_001244960.1      ------------------------------------------------------------
                                                                                

HsCD00513973        GACTCGGAGATCTCCTGCCACATCCAGAGGGAAACCAAAGGGCAGTTTCTCATTGACCAC
NM_174938.5         GACTCGGAGATCTCCTGCCACATCCAGAGGGAAACCAAAGGGCAGTTTCTCATTGACCAC
NM_001244960.1      ------------ATGCAGCTTTCAAAGAGGGAAACCAAAGGGCAGTTTCTCATTGACCAC
                                :   . *: : ..***********************************

HsCD00513973        ATCTGCAACTACTACAGCCTGCTGGAGAAGGACTACTTTGGCATTCGCTATGTGGACCCA
NM_174938.5         ATCTGCAACTACTACAGCCTGCTGGAGAAGGACTACTTTGGCATTCGCTATGTGGACCCA
NM_001244960.1      ATCTGCAACTACTACAGCCTGCTGGAGAAGGACTACTTTGGCATTCGCTATGTGGACCCA
                    ************************************************************

HsCD00513973        GAGAAGCAAAGGCACTGGCTTGAACCTAACAAGTCCATCTTCAAGCAAATGAAAACTCAT
NM_174938.5         GAGAAGCAAAGGCACTGGCTTGAACCTAACAAGTCCATCTTCAAGCAAATGAAAACTCAT
NM_001244960.1      GAGAAGCAAAGGCACTGGCTTGAACCTAACAAGTCCATCTTCAAGCAAATGAAAACTCAT
                    ************************************************************

HsCD00513973        CCACCATACACCATGTGCTTTAGAGTGAAATTCTACCCACATGAACCCTTGAAGATTAAA
NM_174938.5         CCACCATACACCATGTGCTTTAGAGTGAAATTCTACCCACATGAACCCTTGAAGATTAAA
NM_001244960.1      CCACCATACACCATGTGCTTTAGAGTGAAATTCTACCCACATGAACCCTTGAAGATTAAA
                    ************************************************************

HsCD00513973        GAAGAGCTCACAAGATACCTTTTATACCTTCAGATTAAAAGGGACATTTTTCATGGCCAC
NM_174938.5         GAAGAGCTCACAAGATACCTTTTATACCTTCAGATTAAAAGGGACATTTTTCATGGCCGC
NM_001244960.1      GAAGAGCTCACAAGATACCTTTTATACCTTCAGATTAAAAGGGACATTTTTCATGGCCGC
                    **********************************************************.*

HsCD00513973        CTGCTGTGCTCCTTTTCTGATGCTGCCTACCTGGGTGCCTGTATTGTTCAAGCTGAGCTT
NM_174938.5         CTGCTGTGCTCCTTTTCTGATGCTGCCTACCTGGGTGCCTGTATTGTTCAAGCTGAGCTT
NM_001244960.1      CTGCTGTGCTCCTTTTCTGATGCTGCCTACCTGGGTGCCTGTATTGTTCAAGCTGAGCTT
                    ************************************************************

HsCD00513973        GGTGATTACGATCCTAATGAGCATCCTGAGAATTACATCAGTGAGTTTGAGATTTTCCCC
NM_174938.5         GGTGATTACGATCCTGATGAGCATCCTGAGAATTACATCAGTGAGTTTGAGATTTTCCCC
NM_001244960.1      GGTGATTACGATCCTGATGAGCATCCTGAGAATTACATCAGTGAGTTTGAGATTTTCCCC
                    ***************.********************************************

HsCD00513973        AAGCAGTCACAGAAGCTGGAAAGAAAAATAGTGGAAATTCATAAAAATGAACTCAGGGGG
NM_174938.5         AAGCAGTCACAGAAGCTGGAAAGAAAAATAGTGGAAATTCATAAAAATGAACTCAGGGGG
NM_001244960.1      AAGCAGTCACAGAAGCTGGAAAGAAAAATAGTGGAAATTCATAAAAATGAACTCAGGGGG
                    ************************************************************

HsCD00513973        CAGAGCCCACCAGTTGCTGAATTTAACTTGCTCCTGAAAGCTCACACTTTGGAAACCTAC
NM_174938.5         CAGAGCCCACCAGTTGCTGAATTTAACTTGCTCCTGAAAGCTCACACTTTGGAAACCTAC
NM_001244960.1      CAGAGCCCACCAGTTGCTGAATTTAACTTGCTCCTGAAAGCTCACACTTTGGAAACCTAC
                    ************************************************************

HsCD00513973        GGGGTGGATCCTCACCCATGCAAGGATTCAACAGGCACAACAACATTTTTAGGATTCACA
NM_174938.5         GGGGTGGATCCTCACCCATGCAAGGATTCAACAGGCACAACAACATTTTTAGGATTCACA
NM_001244960.1      GGGGTGGATCCTCACCCATGCAAGGATTCAACAGGCACAACAACATTTTTAGGATTCACA
                    ************************************************************

HsCD00513973        GCTGCAGGCTTTGTGGTCTTTCAGGGAAATAAGAGAATCCATTTGATAAAATGGCCAGAT
NM_174938.5         GCTGCAGGCTTTGTGGTCTTTCAGGGAAATAAGAGAATCCATTTGATAAAATGGCCAGAT
NM_001244960.1      GCTGCAGGCTTTGTGGTCTTTCAGGGAAATAAGAGAATCCATTTGATAAAATGGCCAGAT
                    ************************************************************

HsCD00513973        GTCTGCAAATTGAAGTTTGAAGGGAAGACATTTTATGTGATTGGCACCCAGAAGGAGAAA
NM_174938.5         GTCTGCAAATTGAAGTTTGAAGGGAAGACATTTTATGTGATTGGCACCCAGAAGGAGAAA
NM_001244960.1      GTCTGCAAATTGAAGTTTGAAGGGAAGACATTTTATGTGATTGGCACCCAGAAGGAGAAA
                    ************************************************************

HsCD00513973        AAAGCCATGTTGGCATTCAATACTTCAACACCAGCTGCCTGCAAACATCTTTGGAAGTGT
NM_174938.5         AAAGCCATGTTGGCATTCCATACTTCAACACCAGCTGCCTGCAAACATCTTTGGAAGTGT
NM_001244960.1      AAAGCCATGTTGGCATTCCATACTTCAACACCAGCTGCCTGCAAACATCTTTGGAAGTGT
                    ******************.*****************************************

HsCD00513973        GGAGTGGAAAACCAGGCCTTTTATAAGTATGCAAAATCCAGTCAGATCAAGACTGTATCA
NM_174938.5         GGAGTGGAAAACCAGGCCTTTTATAAGTATGCAAAATCCAGTCAGATCAAGACTGTATCA
NM_001244960.1      GGAGTGGAAAACCAGGCCTTTTATAAGTATGCAAAATCCAGTCAGATCAAGACTGTATCA
                    ************************************************************

HsCD00513973        AGCAGCAAGATATTTTTTAAAGGAAGTAGATTTCGATATAGTGGGAAAGTTGCCAAAGAG
NM_174938.5         AGCAGCAAGATATTTTTTAAAGGAAGTAGATTTCGATATAGTGGGAAAGTTGCCAAAGAG
NM_001244960.1      AGCAGCAAGATATTTTTTAAAGGAAGTAGATTTCGATATAGTGGGAAAGTTGCCAAAGAG
                    ************************************************************

HsCD00513973        GTGGTGGAGGCCAGTTCCAAGATCCAGAGGGAGCCTCCTGAGGTGCACAGAGCCAACATT
NM_174938.5         GTGGTGGAGGCCAGTTCCAAGATCCAGAGGGAGCCTCCTGAGGTGCACAGAGCCAACATT
NM_001244960.1      GTGGTGGAGGCCAGTTCCAAGATCCAGAGGGAGCCTCCTGAGGTGCACAGAGCCAACATT
                    ************************************************************

HsCD00513973        ACTCAGAGCCGCAGTTCCCACTCCTTGAACAAACAGCTCATCATTAACATGGAACCCCTG
NM_174938.5         ACTCAGAGCCGCAGTTCCCACTCCTTGAACAAACAGCTCATCATTAACATGGAACCCCTG
NM_001244960.1      ACTCAGAGCCGCAGTTCCCACTCCTTGAACAAACAGCTCATCATTAACATGGAACCCCTG
                    ************************************************************

HsCD00513973        CAGCCCCTGCTTCCTTCCCCCAGCGAGCAAGAAGAAGAACTTCCTCTGGGTGAGGGTGTT
NM_174938.5         CAGCCCCTGCTTCCTTCCCCCAGCGAGCAAGAAGAAGAACTTCCTCTGGGTGAGGGTGTT
NM_001244960.1      CAGCCCCTGCTTCCTTCCCCCAGCGAGCAAGAAGAAGAACTTCCTCTGGGTGAGGGTGTT
                    ************************************************************

HsCD00513973        CCATTGCCTAAAGAGGAGAACATTTCTGCTCCCTTGATCTCCAGCTCCCCAGTGAAGGCA
NM_174938.5         CCATTGCCTAAAGAGGAGAACATTTCTGCTCCCTTGATCTCCAGCTCCCCAGTGAAGGCA
NM_001244960.1      CCATTGCCTAAAGAGGAGAACATTTCTGCTCCCTTGATCTCCAGCTCCCCAGTGAAGGCA
                    ************************************************************

HsCD00513973        GCCCGGGAGTATGAAGATCCCCCTAGTGAAGAGGAAGATAAAATAAAAGAAGAACCTTTA
NM_174938.5         GCCCGGGAGTATGAAGATCCCCCTAGTGAAGAGGAAGATAAAATAAAAGAAGAACCTTTA
NM_001244960.1      GCCCGGGAGTATGAAGATCCCCCTAGTGAAGAGGAAGATAAAATAAAAGAAGAACCTTTA
                    ************************************************************

HsCD00513973        ACCATCTCTGAACTAGTGTACAACCCAAGTGCCAGCCTGCTCCCCACCCCTGTGGATGAC
NM_174938.5         ACCATCTCTGAACTAGTGTACAACCCAAGTGCCAGCCTGCTCCCCACCCCTGTGGATGAC
NM_001244960.1      ACCATCTCTGAACTAGTGTACAACCCAAGTGCCAGCCTGCTCCCCACCCCTGTGGATGAC
                    ************************************************************

HsCD00513973        GATGAGATTGACATGCTCTTTGACTGTCCTTCTAGGCTTGAGTTGGAAAGAGAAGACACA
NM_174938.5         GATGAGATTGACATGCTCTTTGACTGTCCTTCTAGGCTTGAGTTGGAAAGAGAAGACACA
NM_001244960.1      GATGAGATTGACATGCTCTTTGACTGTCCTTCTAGGCTTGAGTTGGAAAGAGAAGACACA
                    ************************************************************

HsCD00513973        GATTCATTTGAGGATCTGGAAGCAGATGAAAACGCCTTTTTGATTGCTGAAGAAGAGGAG
NM_174938.5         GATTCATTTGAGGATCTGGAAGCAGATGAAAACGCCTTTTTGATTGCTGAAGAAGAGGAG
NM_001244960.1      GATTCATTTGAGGATCTGGAAGCAGATGAAAACGCCTTTTTGATTGCTGAAGAAGAGGAG
                    ************************************************************

HsCD00513973        CTGAAGGAGGCTCGCCGTGCTTTGTCGTGGAGCTACGACATTCTGACTGGCCATATTCGG
NM_174938.5         CTGAAGGAGGCTCGCCGTGCTTTGTCGTGGAGCTATGACATTCTGACTGGCCATATTCGG
NM_001244960.1      CTGAAGGAGGCTCGCCGTGCTTTGTCGTGGAGCTATGACATTCTGACTGGCCATATTCGG
                    *********************************** ************************

HsCD00513973        GTGAACCCACTGGTCAAGAGTTTTTCCAGGCTCCTTGTGGTGGGCCTGGGACTGCTGCTC
NM_174938.5         GTGAACCCACTGGTCAAGAGTTTTTCCAGGCTCCTTGTGGTGGGCCTGGGACTGCTGCTC
NM_001244960.1      GTGAACCCACTGGTCAAGAGTTTTTCCAGGCTCCTTGTGGTGGGCCTGGGACTGCTGCTC
                    ************************************************************

HsCD00513973        TTTGTATTTCCCCTGCTCCTCCTCCTTTTGGAGTCAGGTATTGATCTCTCCTTCTTATGC
NM_174938.5         TTTGTATTTCCCCTGCTCCTCCTCCTTTTGGAGTCAGGTATTGATCTCTCCTTCTTATGC
NM_001244960.1      TTTGTATTTCCCCTGCTCCTCCTCCTTTTGGAGTCAGGTATTGATCTCTCCTTCTTATGC
                    ************************************************************

HsCD00513973        GAAATCCGCCAGACACCAGAGTTTGAGCAGTTTCACTATGAATACTACTGTCCCCTCAAG
NM_174938.5         GAAATCCGCCAGACACCAGAGTTTGAGCAGTTTCACTATGAATACTACTGTCCCCTCAAG
NM_001244960.1      GAAATCCGCCAGACACCAGAGTTTGAGCAGTTTCACTATGAATACTACTGTCCCCTCAAG
                    ************************************************************

HsCD00513973        GAGTGGGTGGCTGGGAAAGTCCACCTCATCCTCTACATGCTGGGTTGCTCA---
NM_174938.5         GAGTGGGTGGCTGGGAAAGTCCACCTCATCCTCTACATGCTGGGTTGCTCATGA
NM_001244960.1      GAGTGGGTGGCTGGGAAAGTCCACCTCATCCTCTACATGCTGGGTTGCTCATGA
                    ***************************************************