DNASU Plasmid | TXNDC3 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: NME8

Gene Name: NME/NM23 family member 8

Original Clone ID:

ccsbBroadEn_11973

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC036816.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00514046

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1765nts

Open reading frame: 1 to 1765

Reference Sequence Alignment

ATGGCAAGCAAAAAACGAGAAGTCCAGTTACAGACAGTCATCAATAATCAAAGCCTGTGG
GATGAGATGTTGCAGAACAAAGGCTTAACAGTGATTGATGTTTACCAAGCCTGGTGTGGA
CCTTGCAGAGCAATGCAACCTTTATTCAGAAAATTGAAAAATGAACTGAACGAAGACGAA
ATTCTGCATTTTGCTGTCGCAGAAGCTGACAACATTGTGACTTTGCAGCCATTTAGAGAT
AAATGTGAACCTGTTTTTCTCTTTAGTGTTAATGGCAAAATTATCGAAAAGATTCAGGGT
GCAAATGCACCGCTTGTTAATAAAAAAGTTATTAATTTGATCGATGAGGAGAGAAAAATT
GCAGCAGGTGAAATGGCTCGACCTCAGTATCCTGAAATTCCATTAGTAGACTCAGATTCA
GAAGTTAGTGAAGAATCACCATGTGAAAGTGTTCAGGAATTATACAGTATTGCTATTATC
AAACCGGATGCTGTGATTAGTAAAAAAGTTCTAGAAATTAAAAGAAAAATTACCAAAGCT
GGATTTATTATAGAAGCAGAGCATAAGACAGTGCTCACTGAAGAACAAGTTGTCAACTTC
TATAGTCGAATAGCAGACCAGCGTGACTTCGAAGAGTTTGTCTCTTTTATGACAAGTGGC
TTAAGCTATATTCTAGTTGTATCTCAAGGAAGTAAACACAATCCTCCCTCTGAAGAAACC
GAACCACAGACTGACACCGAACCTAACGAACGATCTGAGGATCAACCTGAGGTCGAAGCC
CAGGTTACACCTGGAATGATGAAGAACAAACAAGACAGTTTACAAGAATATCTGGAAAGA
CAACATTTAGCTCAGCTCTGTGACATTGAAGAGGATGCAGCTAATGTTGCTAAGTTCATG
GATGCTTTCTTCCCCGATTTTAAAAAAATGAAAAGCATGAAATTAGAAAAGACATTGGCA
TTACTTCGACCAAATCTCTTTCATGAAAGGAAAGATGATGTTTTGCGTATTATTAAAGAT
GAAGACTTCAAAATACTGGAGCAAAGACAAGTAGTATTATCGGAAAAAGAAGCACAAGCA
CTGTGCAAGGAATATGAAAATGAAGACTATTTTAATAAACTTATAGAAAACATGACCAGT
GGTCCATCTCTAGCCCTTGTTTTATTGAGAGACAATGGCTTGCAATACTGGAAACAATTA
CTGGGACCAAGAACTGTTGAAGAAGCCATTGAATATTTTCCAGAGAGTTTATGTGCACAG
TTTGCGATGGACAGTTTGCCGGTCAACCAGTTGTATGGCAGCGATTCATTAGAAACCGCT
GAAAGGGAAATACAGCATTTCTTTCCTCTTCAAAGCACTTTAGGCTTGATTAAACCTCAT
GCAACAAGTGAACAAAGAGAGCAGATCCTGAAGATAGTTAAGGAGGCTGGATTTGATCTG
ACACAGGTGAAGAAAATGTTCCTAACTCCTGAGCAAACTGAGAAAATTTATCCAAAAGTA
ACAGGAAAAGACTTTTATAAAGATTTATTGGAAATGTTATCTGTGGGTCCATCTATGGTC
ATGATTCTGACCAAGTGGAATGCTGTTGCAGAATGGAGACGATTGATGGGCCCAACAGAC
CCAGAAGAAGCAAAATTACTTTCCCCTGACTCCATCCGAGCCCAGTTTGGAATAAGTAAA
TTGAAAAACATTGTCCATGGAGCATCTAACGCCTATGAAGCAAAAGAGGTTGTTAATAGA
CTCTTTGAGGATCCTGAGGAAAAC

Protein sequence predicted based on nucleotide sequence

MASKKREVQLQTVINNQSLWDEMLQNKGLTVIDVYQAWCGPCRAMQPLFRKLKNELNEDE
ILHFAVAEADNIVTLQPFRDKCEPVFLFSVNGKIIEKIQGANAPLVNKKVINLIDEERKI
AAGEMARPQYPEIPLVDSDSEVSEESPCESVQELYSIAIIKPDAVISKKVLEIKRKITKA
GFIIEAEHKTVLTEEQVVNFYSRIADQRDFEEFVSFMTSGLSYILVVSQGSKHNPPSEET
EPQTDTEPNERSEDQPEVEAQVTPGMMKNKQDSLQEYLERQHLAQLCDIEEDAANVAKFM
DAFFPDFKKMKSMKLEKTLALLRPNLFHERKDDVLRIIKDEDFKILEQRQVVLSEKEAQA
LCKEYENEDYFNKLIENMTSGPSLALVLLRDNGLQYWKQLLGPRTVEEAIEYFPESLCAQ
FAMDSLPVNQLYGSDSLETAEREIQHFFPLQSTLGLIKPHATSEQREQILKIVKEAGFDL
TQVKKMFLTPEQTEKIYPKVTGKDFYKDLLEMLSVGPSMVMILTKWNAVAEWRRLMGPTD
PEEAKLLSPDSIRAQFGISKLKNIVHGASNAYEAKEVVNRLFEDPEEN

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

NME8

Symbol Nomenclature :

NME8

SYNONYM :

CILD6; NM23-H8; SPTRX2; TXNDC3; sptrx-2

Designation :

sperm-specific thioredoxin 2|spermatid-specific thioredoxin-2|thioredoxin domain containing 3 (spermatozoa)|thioredoxin domain-containing protein 3

Full Nomenclature :

NME/NM23 family member 8

GENEID :

51314

GenBank Accession :

JF432242

HGNC :

16473

MIM :

607421

Vega :

OTTHUMG00000023716

Target GenBank:

JF432242

Reference Sequence Alignment

  

HsCD00514046      ATGGCAAGCAAAAAACGAGAAGTCCAGTTACAGACAGTCATCAATAATCAAAGCCTGTGG
NM_016616.4       ATGGCAAGCAAAAAACGAGAAGTCCAGTTACAGACAGTCATCAATAATCAAAGCCTGTGG
                  ************************************************************

HsCD00514046      GATGAGATGTTGCAGAACAAAGGCTTAACAGTGATTGATGTTTACCAAGCCTGGTGTGGA
NM_016616.4       GATGAGATGTTGCAGAACAAAGGCTTAACAGTGATTGATGTTTACCAAGCCTGGTGTGGA
                  ************************************************************

HsCD00514046      CCTTGCAGAGCAATGCAACCTTTATTCAGAAAATTGAAAAATGAACTGAACGAAGACGAA
NM_016616.4       CCTTGCAGAGCAATGCAACCTTTATTCAGAAAATTGAAAAATGAACTGAACGAAGACGAA
                  ************************************************************

HsCD00514046      ATTCTGCATTTTGCTGTCGCAGAAGCTGACAACATTGTGACTTTGCAGCCATTTAGAGAT
NM_016616.4       ATTCTGCATTTTGCTGTCGCAGAAGCTGACAACATTGTGACTTTGCAGCCATTTAGAGAT
                  ************************************************************

HsCD00514046      AAATGTGAACCTGTTTTTCTCTTTAGTGTTAATGGCAAAATTATCGAAAAGATTCAGGGT
NM_016616.4       AAATGTGAACCTGTTTTTCTCTTTAGTGTTAATGGCAAAATTATCGAAAAGATTCAGGGT
                  ************************************************************

HsCD00514046      GCAAATGCACCGCTTGTTAATAAAAAAGTTATTAATTTGATCGATGAGGAGAGAAAAATT
NM_016616.4       GCAAATGCACCGCTTGTTAATAAAAAAGTTATTAATTTGATCGATGAGGAGAGAAAAATT
                  ************************************************************

HsCD00514046      GCAGCAGGTGAAATGGCTCGACCTCAGTATCCTGAAATTCCATTAGTAGACTCAGATTCA
NM_016616.4       GCAGCAGGTGAAATGGCTCGACCTCAGTATCCTGAAATTCCATTAGTAGACTCAGATTCA
                  ************************************************************

HsCD00514046      GAAGTTAGTGAAGAATCACCATGTGAAAGTGTTCAGGAATTATACAGTATTGCTATTATC
NM_016616.4       GAAGTTAGTGAAGAATCACCATGTGAAAGTGTTCAGGAATTATACAGTATTGCTATTATC
                  ************************************************************

HsCD00514046      AAACCGGATGCTGTGATTAGTAAAAAAGTTCTAGAAATTAAAAGAAAAATTACCAAAGCT
NM_016616.4       AAACCGGATGCTGTGATTAGTAAAAAAGTTCTAGAAATTAAAAGAAAAATTACCAAAGCT
                  ************************************************************

HsCD00514046      GGATTTATTATAGAAGCAGAGCATAAGACAGTGCTCACTGAAGAACAAGTTGTCAACTTC
NM_016616.4       GGATTTATTATAGAAGCAGAGCATAAGACAGTGCTCACTGAAGAACAAGTTGTCAACTTC
                  ************************************************************

HsCD00514046      TATAGTCGAATAGCAGACCAGCGTGACTTCGAAGAGTTTGTCTCTTTTATGACAAGTGGC
NM_016616.4       TATAGTCGAATAGCAGACCAGTGTGACTTCGAAGAGTTTGTCTCTTTTATGACAAGTGGC
                  ********************* **************************************

HsCD00514046      TTAAGCTATATTCTAGTTGTATCTCAAGGAAGTAAACACAATCCTCCCTCTGAAGAAACC
NM_016616.4       TTAAGCTATATTCTAGTTGTATCTCAAGGAAGTAAACACAATCCTCCCTCTGAAGAAACC
                  ************************************************************

HsCD00514046      GAACCACAGACTGACACCGAACCTAACGAACGATCTGAGGATCAACCTGAGGTCGAAGCC
NM_016616.4       GAACCACAGACTGACACCGAACCTAACGAACGATCTGAGGATCAACCTGAGGTCGAAGCC
                  ************************************************************

HsCD00514046      CAGGTTACACCTGGAATGATGAAGAACAAACAAGACAGTTTACAAGAATATCTGGAAAGA
NM_016616.4       CAGGTTACACCTGGAATGATGAAGAACAAACAAGACAGTTTACAAGAATATCTGGAAAGA
                  ************************************************************

HsCD00514046      CAACATTTAGCTCAGCTCTGTGACATTGAAGAGGATGCAGCTAATGTTGCTAAGTTCATG
NM_016616.4       CAACATTTAGCTCAGCTCTGTGACATTGAAGAGGATGCAGCTAATGTTGCTAAGTTCATG
                  ************************************************************

HsCD00514046      GATGCTTTCTTCCCCGATTTTAAAAAAATGAAAAGCATGAAATTAGAAAAGACATTGGCA
NM_016616.4       GATGCTTTCTTCCCCGATTTTAAAAAAATGAAAAGCATGAAATTAGAAAAGACATTGGCA
                  ************************************************************

HsCD00514046      TTACTTCGACCAAATCTCTTTCATGAAAGGAAAGATGATGTTTTGCGTATTATTAAAGAT
NM_016616.4       TTACTTCGACCAAATCTCTTTCATGAAAGGAAAGATGATGTTTTGCGTATTATTAAAGAT
                  ************************************************************

HsCD00514046      GAAGACTTCAAAATACTGGAGCAAAGACAAGTAGTATTATCGGAAAAAGAAGCACAAGCA
NM_016616.4       GAAGACTTCAAAATACTGGAGCAAAGACAAGTAGTATTATCGGAAAAAGAAGCACAAGCA
                  ************************************************************

HsCD00514046      CTGTGCAAGGAATATGAAAATGAAGACTATTTTAATAAACTTATAGAAAACATGACCAGT
NM_016616.4       CTGTGCAAGGAATATGAAAATGAAGACTATTTTAATAAACTTATAGAAAACATGACCAGT
                  ************************************************************

HsCD00514046      GGTCCATCTCTAGCCCTTGTTTTATTGAGAGACAATGGCTTGCAATACTGGAAACAATTA
NM_016616.4       GGTCCATCTCTAGCCCTTGTTTTATTGAGAGACAATGGCTTGCAATACTGGAAACAATTA
                  ************************************************************

HsCD00514046      CTGGGACCAAGAACTGTTGAAGAAGCCATTGAATATTTTCCAGAGAGTTTATGTGCACAG
NM_016616.4       CTGGGACCAAGAACTGTTGAAGAAGCCATTGAATATTTTCCAGAGAGTTTATGTGCACAG
                  ************************************************************

HsCD00514046      TTTGCGATGGACAGTTTGCCGGTCAACCAGTTGTATGGCAGCGATTCATTAGAAACCGCT
NM_016616.4       TTTGCGATGGACAGTTTGCCGGTCAACCAGTTGTATGGCAGCGATTCATTAGAAACCGCT
                  ************************************************************

HsCD00514046      GAAAGGGAAATACAGCATTTCTTTCCTCTTCAAAGCACTTTAGGCTTGATTAAACCTCAT
NM_016616.4       GAAAGGGAAATACAGCATTTCTTTCCTCTTCAAAGCACTTTAGGCTTGATTAAACCTCAT
                  ************************************************************

HsCD00514046      GCAACAAGTGAACAAAGAGAGCAGATCCTGAAGATAGTTAAGGAGGCTGGATTTGATCTG
NM_016616.4       GCAACAAGTGAACAAAGAGAGCAGATCCTGAAGATAGTTAAGGAGGCTGGATTTGATCTG
                  ************************************************************

HsCD00514046      ACACAGGTGAAGAAAATGTTCCTAACTCCTGAGCAAACTGAGAAAATTTATCCAAAAGTA
NM_016616.4       ACACAGGTGAAGAAAATGTTCCTAACTCCTGAGCAAATAGAGAAAATTTATCCAAAAGTA
                  ************************************* :*********************

HsCD00514046      ACAGGAAAAGACTTTTATAAAGATTTATTGGAAATGTTATCTGTGGGTCCATCTATGGTC
NM_016616.4       ACAGGAAAAGACTTTTATAAAGATTTATTGGAAATGTTATCTGTGGGTCCATCTATGGTC
                  ************************************************************

HsCD00514046      ATGATTCTGACCAAGTGGAATGCTGTTGCAGAATGGAGACGATTGATGGGCCCAACAGAC
NM_016616.4       ATGATTCTGACCAAGTGGAATGCTGTTGCAGAATGGAGACGATTGATGGGCCCAACAGAC
                  ************************************************************

HsCD00514046      CCAGAAGAAGCAAAATTACTTTCCCCTGACTCCATCCGAGCCCAGTTTGGAATAAGTAAA
NM_016616.4       CCAGAAGAAGCAAAATTACTTTCCCCTGACTCCATCCGAGCCCAGTTTGGAATAAGTAAA
                  ************************************************************

HsCD00514046      TTGAAAAACATTGTCCATGGAGCATCTAACGCCTATGAAGCAAAAGAGGTTGTTAATAGA
NM_016616.4       TTGAAAAACATTGTCCATGGAGCATCTAACGCCTATGAAGCAAAAGAGGTTGTTAATAGA
                  ************************************************************

HsCD00514046      CTCTTTGAGGATCCTGAGGAAAAC---
NM_016616.4       CTCTTTGAGGATCCTGAGGAAAACTAA
                  ************************