DNASU Plasmid | KLHL22 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: KLHL22

Gene Name: kelch-like family member 22

Original Clone ID:

ccsbBroadEn_04434

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC015923.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00514314

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1903nts

Open reading frame: 1 to 1903

Reference Sequence Alignment

ATGGCAGAGGAGCAGGAGTTCACCCAGCTCTGCAAGTTGCCTGCACAGCCCTCACACCCA
CACTGCGTGAACAACACCTACCGCAGCGCACAGCACTCCCAGGCTCTGCTCCGAGGGCTG
CTGGCTCTCCGGGACAGCGGAATCCTCTTCGATGTTGTGCTGGTGGTGGAGGGCAGACAC
ATCGAGGCCCATCGCATCCTGCTGGCTGCGTCCTGCGATTACTTCAGAGGAATGTTTGCT
GGGGGATTGAAGGAGATGGAACAGGAAGAGGTCCTGATCCACGGTGTGTCCTACAATGCT
ATGTGCCAAATCCTACATTTCATATACACCTCCGAGCTGGAGCTCAGCCTGAGCAATGTA
CAAGAGACACTGGTGGCTGCCTGCCAGCTTCAGATCCCAGAAATTATCCATTTCTGCTGT
GATTTCCTCATGTCCTGGGTGGACGAAGAGAACATTCTCGATGTCTACCGGCTGGCAGAG
CTGTTTGACTTGAGCCGCCTGACTGAGCAACTGGACACCTATATCCTCAAAAACTTTGTG
GCCTTCTCTCGGACTGACAAGTACCGCCAGCTTCCATTGGAGAAGGTCTACTCCCTCCTC
AGCAGCAATCGCCTGGAGGTCTCCTGCGAGACCGAGGTATATGAGGGGGCCCTTCTCTAC
CATTATAGCCTGGAGCAGGTGCAGGCTGACCAGATCTCGCTGCACGAGCCCCCAAAGCTC
CTTGAGACAGTGCGGTTTCCGCTGATGGAAGCTGAGGTCCTGCAGCGGCTGCATGACAAG
CTGGACCCCAGCCCTTTGAGGGACACAGTGGCCAGCGCCCTCATGTACCACCGGAACGAG
AGCCTACAGCCCAGCCTGCAGAGCCCGCAAACGGAGCTGCGGTCGGACTTCCAGTGCGTT
GTGGGCTTCGGGGGCATTCACTCCACGCCGTCCACTGTCCTCAGCGACCAGGCCAAGTAT
CTAAACCCCTTACTGGGAGAGTGGAAGCACTTCACTGCCTCCCTGGCCCCCCGCATGTCC
AACCAGGGCATCGCGGTGCTCAACAACTTCGTATACTTGATTGGAGGGGACAACAATGTC
CAAGGATTTCGAGCAGAGTCCCGATGCTGGAGGTATGACCCACGGCACAACCGCTGGTTC
CAGATCCAGTCCCTGCAGCAGGAGCACGCCGACCTGTCCGTGTGTGTTGTAGGCAGGTAC
ATCTACGCTGTGGCGGGCCGTGACTACCACAATGACCTGAATGCTGTGGAGCGCTACGAC
CCTGCCACCAACTCCTGGGCATACGTGGCCCCACTCAAGAGGGAGGTGTATGCCCACGCA
GGCGCGACGCTGGAGGGGAAGATGTATATCACCTGCGGCCGCAGAGGGGAGGATTACCTG
AAAGAGACACACTGCTACGATCCAGGCAGCAACACTTGGCACACACTGGCTGATGGGCCT
GTGCGGCGCGCCTGGCACGGCATGGCAACCCTCCTCAACAAGCTGTATGTGATCGGGGGC
AGCAACAACGATGCCGGATACAGGAGGGACGTGCACCAGGTGGCCTGCTACAGCTGCACG
TCTGGACAGTGGTCATCTGTCTGCCCACTCCCTGCTGGGCACGGTGAGCCTGGCATTGCT
GTGCTGGACAACAGGATCTATGTGTTAGGTGGCCGCTCACACAACCGCGGCAGCCGCACA
GGCTACGTGCACATTTACGATGTGGAGAAGGACTGCTGGGAGGAAGGGCCCCAGCTGGAC
AACTCCATCTCAGGCCTGGCGGCCTGTGTGCTCACCCTGCCCCGCTCCCTGCTCCTTGAG
CCGCCCCGCGGGACCCCTGACCGCAGCCAGGCCGACCCGGACTTTGCCTCTGAGGTGATG
AGTGTGTCTGACTGGGAGGAGTTTGACAACTCCAGTGAGGAC

Protein sequence predicted based on nucleotide sequence

MAEEQEFTQLCKLPAQPSHPHCVNNTYRSAQHSQALLRGLLALRDSGILFDVVLVVEGRH
IEAHRILLAASCDYFRGMFAGGLKEMEQEEVLIHGVSYNAMCQILHFIYTSELELSLSNV
QETLVAACQLQIPEIIHFCCDFLMSWVDEENILDVYRLAELFDLSRLTEQLDTYILKNFV
AFSRTDKYRQLPLEKVYSLLSSNRLEVSCETEVYEGALLYHYSLEQVQADQISLHEPPKL
LETVRFPLMEAEVLQRLHDKLDPSPLRDTVASALMYHRNESLQPSLQSPQTELRSDFQCV
VGFGGIHSTPSTVLSDQAKYLNPLLGEWKHFTASLAPRMSNQGIAVLNNFVYLIGGDNNV
QGFRAESRCWRYDPRHNRWFQIQSLQQEHADLSVCVVGRYIYAVAGRDYHNDLNAVERYD
PATNSWAYVAPLKREVYAHAGATLEGKMYITCGRRGEDYLKETHCYDPGSNTWHTLADGP
VRRAWHGMATLLNKLYVIGGSNNDAGYRRDVHQVACYSCTSGQWSSVCPLPAGHGEPGIA
VLDNRIYVLGGRSHNRGSRTGYVHIYDVEKDCWEEGPQLDNSISGLAACVLTLPRSLLLE
PPRGTPDRSQADPDFASEVMSVSDWEEFDNSSED

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

KLHL22

Symbol Nomenclature :

KLHL22

SYNONYM :

KELCHL

Designation :

kelch-like 22|kelch-like protein 22

Full Nomenclature :

kelch-like family member 22

GENEID :

84861

GenBank Accession :

CU013350

HGNC :

25888

Vega :

OTTHUMG00000150778

Target GenBank:

CU013350

Reference Sequence Alignment

  

HsCD00514314      ATGGCAGAGGAGCAGGAGTTCACCCAGCTCTGCAAGTTGCCTGCACAGCCCTCACACCCA
NM_032775.3       ATGGCAGAGGAGCAGGAGTTCACCCAGCTCTGCAAGTTGCCTGCACAGCCCTCACACCCA
                  ************************************************************

HsCD00514314      CACTGCGTGAACAACACCTACCGCAGCGCACAGCACTCCCAGGCTCTGCTCCGAGGGCTG
NM_032775.3       CACTGCGTGAACAACACCTACCGCAGCGCACAGCACTCCCAGGCTCTGCTCCGAGGGCTG
                  ************************************************************

HsCD00514314      CTGGCTCTCCGGGACAGCGGAATCCTCTTCGATGTTGTGCTGGTGGTGGAGGGCAGACAC
NM_032775.3       CTGGCTCTCCGGGACAGCGGAATCCTCTTCGATGTTGTGCTGGTGGTGGAGGGCAGACAC
                  ************************************************************

HsCD00514314      ATCGAGGCCCATCGCATCCTGCTGGCTGCGTCCTGCGATTACTTCAGAGGAATGTTTGCT
NM_032775.3       ATCGAGGCCCATCGCATCCTGCTGGCTGCGTCCTGCGATTACTTCAGAGGAATGTTTGCT
                  ************************************************************

HsCD00514314      GGGGGATTGAAGGAGATGGAACAGGAAGAGGTCCTGATCCACGGTGTGTCCTACAATGCT
NM_032775.3       GGGGGATTGAAGGAGATGGAACAGGAAGAGGTCCTGATCCACGGTGTGTCCTACAATGCT
                  ************************************************************

HsCD00514314      ATGTGCCAAATCCTACATTTCATATACACCTCCGAGCTGGAGCTCAGCCTGAGCAATGTA
NM_032775.3       ATGTGCCAAATCCTACATTTCATATACACCTCCGAGCTGGAGCTCAGCCTGAGCAATGTA
                  ************************************************************

HsCD00514314      CAAGAGACACTGGTGGCTGCCTGCCAGCTTCAGATCCCAGAAATTATCCATTTCTGCTGT
NM_032775.3       CAAGAGACACTGGTGGCTGCCTGCCAGCTTCAGATCCCAGAAATTATCCATTTCTGCTGT
                  ************************************************************

HsCD00514314      GATTTCCTCATGTCCTGGGTGGACGAAGAGAACATTCTCGATGTCTACCGGCTGGCAGAG
NM_032775.3       GATTTCCTCATGTCCTGGGTGGACGAAGAGAACATTCTCGATGTCTACCGGCTGGCAGAG
                  ************************************************************

HsCD00514314      CTGTTTGACTTGAGCCGCCTGACTGAGCAACTGGACACCTATATCCTCAAAAACTTTGTG
NM_032775.3       CTGTTTGACTTGAGCCGCCTGACTGAGCAACTGGACACCTATATCCTCAAAAACTTTGTG
                  ************************************************************

HsCD00514314      GCCTTCTCTCGGACTGACAAGTACCGCCAGCTTCCATTGGAGAAGGTCTACTCCCTCCTC
NM_032775.3       GCCTTCTCTCGGACTGACAAGTACCGCCAGCTTCCATTGGAGAAGGTCTACTCCCTCCTC
                  ************************************************************

HsCD00514314      AGCAGCAATCGCCTGGAGGTCTCCTGCGAGACCGAGGTATATGAGGGGGCCCTTCTCTAC
NM_032775.3       AGCAGCAATCGCCTGGAGGTCTCCTGCGAGACCGAGGTATATGAGGGGGCCCTTCTCTAC
                  ************************************************************

HsCD00514314      CATTATAGCCTGGAGCAGGTGCAGGCTGACCAGATCTCGCTGCACGAGCCCCCAAAGCTC
NM_032775.3       CATTATAGCCTGGAGCAGGTGCAGGCTGACCAGATCTCGCTGCACGAGCCCCCAAAGCTC
                  ************************************************************

HsCD00514314      CTTGAGACAGTGCGGTTTCCGCTGATGGAAGCTGAGGTCCTGCAGCGGCTGCATGACAAG
NM_032775.3       CTTGAGACAGTGCGGTTTCCGCTGATGGAAGCTGAGGTCCTGCAGCGGCTGCATGACAAG
                  ************************************************************

HsCD00514314      CTGGACCCCAGCCCTTTGAGGGACACAGTGGCCAGCGCCCTCATGTACCACCGGAACGAG
NM_032775.3       CTGGACCCCAGCCCTTTGAGGGACACAGTGGCCAGCGCCCTCATGTACCACCGGAACGAG
                  ************************************************************

HsCD00514314      AGCCTACAGCCCAGCCTGCAGAGCCCGCAAACGGAGCTGCGGTCGGACTTCCAGTGCGTT
NM_032775.3       AGCCTACAGCCCAGCCTGCAGAGCCCGCAAACGGAGCTGCGGTCGGACTTCCAGTGCGTT
                  ************************************************************

HsCD00514314      GTGGGCTTCGGGGGCATTCACTCCACGCCGTCCACTGTCCTCAGCGACCAGGCCAAGTAT
NM_032775.3       GTGGGCTTCGGGGGCATTCACTCCACGCCGTCCACTGTCCTCAGCGACCAGGCCAAGTAT
                  ************************************************************

HsCD00514314      CTAAACCCCTTACTGGGAGAGTGGAAGCACTTCACTGCCTCCCTGGCCCCCCGCATGTCC
NM_032775.3       CTAAACCCCTTACTGGGAGAGTGGAAGCACTTCACTGCCTCCCTGGCCCCCCGCATGTCC
                  ************************************************************

HsCD00514314      AACCAGGGCATCGCGGTGCTCAACAACTTCGTATACTTGATTGGAGGGGACAACAATGTC
NM_032775.3       AACCAGGGCATCGCGGTGCTCAACAACTTCGTATACTTGATTGGAGGGGACAACAATGTC
                  ************************************************************

HsCD00514314      CAAGGATTTCGAGCAGAGTCCCGATGCTGGAGGTATGACCCACGGCACAACCGCTGGTTC
NM_032775.3       CAAGGATTTCGAGCAGAGTCCCGATGCTGGAGGTATGACCCACGGCACAACCGCTGGTTC
                  ************************************************************

HsCD00514314      CAGATCCAGTCCCTGCAGCAGGAGCACGCCGACCTGTCCGTGTGTGTTGTAGGCAGGTAC
NM_032775.3       CAGATCCAGTCCCTGCAGCAGGAGCACGCCGACCTGTCCGTGTGTGTTGTAGGCAGGTAC
                  ************************************************************

HsCD00514314      ATCTACGCTGTGGCGGGCCGTGACTACCACAATGACCTGAATGCTGTGGAGCGCTACGAC
NM_032775.3       ATCTACGCTGTGGCGGGCCGTGACTACCACAATGACCTGAATGCTGTGGAGCGCTACGAC
                  ************************************************************

HsCD00514314      CCTGCCACCAACTCCTGGGCATACGTGGCCCCACTCAAGAGGGAGGTGTATGCCCACGCA
NM_032775.3       CCTGCCACCAACTCCTGGGCATACGTGGCCCCACTCAAGAGGGAGGTGTATGCCCACGCA
                  ************************************************************

HsCD00514314      GGCGCGACGCTGGAGGGGAAGATGTATATCACCTGCGGCCGCAGAGGGGAGGATTACCTG
NM_032775.3       GGCGCGACGCTGGAGGGGAAGATGTATATCACCTGCGGCCGCAGAGGGGAGGATTACCTG
                  ************************************************************

HsCD00514314      AAAGAGACACACTGCTACGATCCAGGCAGCAACACTTGGCACACACTGGCTGATGGGCCT
NM_032775.3       AAAGAGACACACTGCTACGATCCAGGCAGCAACACTTGGCACACACTGGCTGATGGGCCT
                  ************************************************************

HsCD00514314      GTGCGGCGCGCCTGGCACGGCATGGCAACCCTCCTCAACAAGCTGTATGTGATCGGGGGC
NM_032775.3       GTGCGGCGCGCCTGGCACGGCATGGCAACCCTCCTCAACAAGCTGTATGTGATCGGGGGC
                  ************************************************************

HsCD00514314      AGCAACAACGATGCCGGATACAGGAGGGACGTGCACCAGGTGGCCTGCTACAGCTGCACG
NM_032775.3       AGCAACAACGATGCCGGATACAGGAGGGACGTGCACCAGGTGGCCTGCTACAGCTGCACG
                  ************************************************************

HsCD00514314      TCTGGACAGTGGTCATCTGTCTGCCCACTCCCTGCTGGGCACGGTGAGCCTGGCATTGCT
NM_032775.3       TCTGGACAGTGGTCATCTGTCTGCCCACTCCCTGCTGGGCACGGTGAGCCTGGCATTGCT
                  ************************************************************

HsCD00514314      GTGCTGGACAACAGGATCTATGTGTTAGGTGGCCGCTCACACAACCGCGGCAGCCGCACA
NM_032775.3       GTGCTGGACAACAGGATCTATGTGTTAGGTGGCCGCTCACACAACCGCGGCAGCCGCACA
                  ************************************************************

HsCD00514314      GGCTACGTGCACATTTACGATGTGGAGAAGGACTGCTGGGAGGAAGGGCCCCAGCTGGAC
NM_032775.3       GGCTACGTGCACATTTACGATGTGGAGAAGGACTGCTGGGAGGAAGGGCCCCAGCTGGAC
                  ************************************************************

HsCD00514314      AACTCCATCTCAGGCCTGGCGGCCTGTGTGCTCACCCTGCCCCGCTCCCTGCTCCTTGAG
NM_032775.3       AACTCCATCTCAGGCCTGGCGGCCTGTGTGCTCACCCTGCCCCGCTCCCTGCTCCTTGAG
                  ************************************************************

HsCD00514314      CCGCCCCGCGGGACCCCTGACCGCAGCCAGGCCGACCCGGACTTTGCCTCTGAGGTGATG
NM_032775.3       CCGCCCCGCGGGACCCCTGACCGCAGCCAGGCCGACCCGGACTTTGCCTCTGAGGTGATG
                  ************************************************************

HsCD00514314      AGTGTGTCTGACTGGGAGGAGTTTGACAACTCCAGTGAGGAC---
NM_032775.3       AGTGTGTCTGACTGGGAGGAGTTTGACAACTCCAGTGAGGACTAG
                  ******************************************