DNASU Plasmid | KLHL34 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: KLHL34

Gene Name: kelch-like family member 34

Original Clone ID:

ccsbBroadEn_09923

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC109031.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00514421

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1933nts

Open reading frame: 1 to 1933

Reference Sequence Alignment

ATGAGTTACTTCCTGTCTTACTGCAAAGCTCATGGCGGCGCGCTGCTCACCGGCTACCAG
GCCCTGCGCGCCGAGGGCTTCCTGTGCGACGTGACACTGGAGACCGAGGGCAGCGAATTC
CCGGCGCACAGGTCGCTCCTGGCGTGCTCCAGTGACTACTTCAGGGCCCTGTTCAAGAGC
CACACCCAGGAATCCCGGGCGCGCGTGATCCACCTGCACGTGCCATCGGCAGCCGGCCTG
CAGCGCCTGCTGGACTTCATCTACACTGCCTGGCTGTCGCTTTCCATGGACACTGTAGAG
GACACTCTGGAGGCCGCCAGCTACCTGCAGGTCACTGAGGCCCTGGGGCTCTGTGGGCGC
TACTTGGAGCGCCAGCTGGCTCCAGAGAACTGCTGCTTCGCCGCCAACGTGGCAGCGCGC
TTTGGCCTGGCTCACACGCTGGACGCGGCCGAGCGCTGCATCGTGAGCCACTTGCAGGAG
CTGCTGGCGCGGGGCGCGGGCCCCGCGGGACTCCTGGAGCTCAACCCTACATCGCTGAGG
GCTGTACTGGGTGCCCCCGACGTGGCGCGGGTGCCCGAGGCCCGGCTACTGGGCCTGGCG
TTAGCTTGGTTGCGGCAGGAGCCCACAACTGAGCGCCTGGCACACTGTACAGAGTTGCTG
GAGCGTGTCCGCTTTGGCCTGGTTCCCGCCGACGTACTGCGGCGCGTGTACTCGGGCTCT
GGCCTCGTGCTGCCCGCCCGGGTCAAGGGCCTCATCATCCAGGCCCTCAACTACCACACG
ACGCCCTCCCGCCAGCCGCTCATGCAGGGCGAGCAGACCAGCATCCGGAGCCCCCAGACC
CGCATCTTGTTGGTGGGGGGGCGCAGGGCACGGGAGGTGGTGATTGAGGAGGTCGCGGCC
CCGCAGAGGGCAGCTAGGGGCCAGGTCGCCGCCCCAGAGCCCGAGGAAGAAGAGGAAGAG
TTGGAGGAAGAGGAGGAGGAGGAGGAGTGGGAGCTCACCCAGAACGTGGTGGCCTTCGAT
GTGTACAATCACCGCTGGCGCAGCCTTACGCAGCTACCCACACCACTGCTGGGGCACAGC
GTGTGCACCGCGGGCAACTTCCTGTTTGTCCTGGGTGGGGAGAGCCCTTCCGGCAGTGCA
TCCTCTCCCCTGGCCGACGACTCGCGGGTGGTCACGGCCCAAGTGCACCGTTACGACCCG
CGCTTCCACGCTTGGACGGAAGTGCCCGCCATGCGGGAAGCGCGGGCCCACTTCTGGTGC
GGCGCGGTGGGCGAGAGGCTCCTGGCCGTCGGGGGCCTGGGTGCGGGCGGTGAGGTGCTG
GCCTCGGTGGAGATGTACGACCTGCGTCGGGACCGCTGGACGGCGGCTGGGGCACTACCG
CGGGCTCTGCACGGTCACGCGGGGGCCGTCGGGGACCGCGGTGTTGTGTACATCTCGGGG
GGCAAGGCAGGGAGAGGCGAGGGCGGAGCGAGCAGCCTCCGGGACTTATACGTCCTGGGC
CCTGAGGAGCAGGTTTGGAGCAAGAAGGCACCCATGGGCACCGCACGTTTCGGGCACCAC
ATGGCAGTGCTGCGCGGCGCTGTGTTTGCTTTTCTGGGGCGATACGAGCCCTTCTCTGAG
ATCGAGCGCTACGACCCCGGCGCCGACCAGTGGACTCGGTTGCGGCCGCTGCCCTACGAC
CGCTTCTGCTATGGGCTGGCCGTGGTCGAGGAGACAGCGTTGCTGCTGGGCGGCCTCAAG
TGGCGGGACTCGCGCCAGGTGCCTACCCGCAACGTAGTGGGCTACGACCTCGACCTGGAC
CGTTGGGAGGACATCGGCTGCGCGTTGCCCTGGGCCTGGAGCGGCCTGCGGTGCGCAGTG
CTGCAGCTGGCCGAGGGTGGGGACGACGAGAGGGAGGGAGAGGTTGGAGAGGCGCTAGAT
TTGGTGCTGGGC

Protein sequence predicted based on nucleotide sequence

MSYFLSYCKAHGGALLTGYQALRAEGFLCDVTLETEGSEFPAHRSLLACSSDYFRALFKS
HTQESRARVIHLHVPSAAGLQRLLDFIYTAWLSLSMDTVEDTLEAASYLQVTEALGLCGR
YLERQLAPENCCFAANVAARFGLAHTLDAAERCIVSHLQELLARGAGPAGLLELNPTSLR
AVLGAPDVARVPEARLLGLALAWLRQEPTTERLAHCTELLERVRFGLVPADVLRRVYSGS
GLVLPARVKGLIIQALNYHTTPSRQPLMQGEQTSIRSPQTRILLVGGRRAREVVIEEVAA
PQRAARGQVAAPEPEEEEEELEEEEEEEEWELTQNVVAFDVYNHRWRSLTQLPTPLLGHS
VCTAGNFLFVLGGESPSGSASSPLADDSRVVTAQVHRYDPRFHAWTEVPAMREARAHFWC
GAVGERLLAVGGLGAGGEVLASVEMYDLRRDRWTAAGALPRALHGHAGAVGDRGVVYISG
GKAGRGEGGASSLRDLYVLGPEEQVWSKKAPMGTARFGHHMAVLRGAVFAFLGRYEPFSE
IERYDPGADQWTRLRPLPYDRFCYGLAVVEETALLLGGLKWRDSRQVPTRNVVGYDLDLD
RWEDIGCALPWAWSGLRCAVLQLAEGGDDEREGEVGEALDLVLG

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

KLHL34

Symbol Nomenclature :

KLHL34

Designation :

kelch-like 34|kelch-like protein 34

Full Nomenclature :

kelch-like family member 34

GENEID :

257240

Locus Tag :

RP11-450P7.3

HGNC :

26634

Vega :

OTTHUMG00000021234

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00514421      ATGAGTTACTTCCTGTCTTACTGCAAAGCTCATGGCGGCGCGCTGCTCACCGGCTACCAG
NM_153270.1       ATGAGTTACTTCCTGTCTTACTGCAAAGCTCATGGCGGCGCGCTGCTCACCGGCTACCAG
                  ************************************************************

HsCD00514421      GCCCTGCGCGCCGAGGGCTTCCTGTGCGACGTGACACTGGAGACCGAGGGCAGCGAATTC
NM_153270.1       GCCCTGCGCGCCGAGGGCTTCCTGTGCGACGTGACACTGGAGACCGAGGGCAGCGAATTC
                  ************************************************************

HsCD00514421      CCGGCGCACAGGTCGCTCCTGGCGTGCTCCAGTGACTACTTCAGGGCCCTGTTCAAGAGC
NM_153270.1       CCGGCGCACAGGTCGCTCCTGGCGTGCTCCAGTGACTACTTCAGGGCCCTGTTCAAGAGC
                  ************************************************************

HsCD00514421      CACACCCAGGAATCCCGGGCGCGCGTGATCCACCTGCACGTGCCATCGGCAGCCGGCCTG
NM_153270.1       CACACCCAGGAATCCCGGGCGCGCGTGATCCACCTGCACGTGCCATCGGCAGCCGGCCTG
                  ************************************************************

HsCD00514421      CAGCGCCTGCTGGACTTCATCTACACTGCCTGGCTGTCGCTTTCCATGGACACTGTAGAG
NM_153270.1       CAGCGCCTGCTGGACTTCATCTACACTGCCTGGCTGTCGCTTTCCATGGACACTGTAGAG
                  ************************************************************

HsCD00514421      GACACTCTGGAGGCCGCCAGCTACCTGCAGGTCACTGAGGCCCTGGGGCTCTGTGGGCGC
NM_153270.1       GACACTCTGGAGGCCGCCAGCTACCTGCAGGTCACTGAGGCCCTGGGGCTCTGTGGGCGC
                  ************************************************************

HsCD00514421      TACTTGGAGCGCCAGCTGGCTCCAGAGAACTGCTGCTTCGCCGCCAACGTGGCAGCGCGC
NM_153270.1       TACTTGGAGCGCCAGCTGGCTCCAGAGAACTGCTGCTTCGCCGCCAACGTGGCAGCGCGC
                  ************************************************************

HsCD00514421      TTTGGCCTGGCTCACACGCTGGACGCGGCCGAGCGCTGCATCGTGAGCCACTTGCAGGAG
NM_153270.1       TTTGGCCTGGCTCACACGCTGGACGCGGCCGAGCGCTGCATCGTGAGCCACTTGCAGGAG
                  ************************************************************

HsCD00514421      CTGCTGGCGCGGGGCGCGGGCCCCGCGGGACTCCTGGAGCTCAACCCTACATCGCTGAGG
NM_153270.1       CTGCTGGCGCGGGGCGCGGGCCCCGCGGGACTCCTGGAGCTCAACCCTACATCGCTGAGG
                  ************************************************************

HsCD00514421      GCTGTACTGGGTGCCCCCGACGTGGCGCGGGTGCCCGAGGCCCGGCTACTGGGCCTGGCG
NM_153270.1       GCTGTACTGGGTGCCCCCGACGTGGCGCGGGTGCCCGAGGCCCGGCTACTGGGCCTGGCG
                  ************************************************************

HsCD00514421      TTAGCTTGGTTGCGGCAGGAGCCCACAACTGAGCGCCTGGCACACTGTACAGAGTTGCTG
NM_153270.1       TTAGCTTGGTTGCGGCAGGAGCCCACAACTGAGCGCCTGGCACACTGTACAGAGTTGCTG
                  ************************************************************

HsCD00514421      GAGCGTGTCCGCTTTGGCCTGGTTCCCGCCGACGTACTGCGGCGCGTGTACTCGGGCTCT
NM_153270.1       GAGCGTGTCCGCTTTGGCCTGGTTCCCGCCGACGTACTGCGGCGCGTGTACTCGGGCTCT
                  ************************************************************

HsCD00514421      GGCCTCGTGCTGCCCGCCCGGGTCAAGGGCCTCATCATCCAGGCCCTCAACTACCACACG
NM_153270.1       GGCCTCGTGCTGCCCGCCCGGGTCAAGGGCCTCATCATCCAGGCCCTCAACTACCACACG
                  ************************************************************

HsCD00514421      ACGCCCTCCCGCCAGCCGCTCATGCAGGGCGAGCAGACCAGCATCCGGAGCCCCCAGACC
NM_153270.1       ACGCCCTCCCGCCAGCCGCTCATGCAGGGCGAGCAGACCAGCATCCGGAGCCCCCAGACC
                  ************************************************************

HsCD00514421      CGCATCTTGTTGGTGGGGGGGCGCAGGGCACGGGAGGTGGTGATTGAGGAGGTCGCGGCC
NM_153270.1       CGCATCTTGTTGGTGGGGGGGCGCAGGGCACGGGAGGTGGTGATTGAGGAGGTCGCGGCC
                  ************************************************************

HsCD00514421      CCGCAGAGGGCAGCTAGGGGCCAGGTCGCCGCCCCAGAGCCCGAGGAAGAAGAGGAAGAG
NM_153270.1       CCGCAGAGGGCAGCTAGGGGCCAGGTCGCCGCCCCAGAGCCCGAGGAAGAAGAGGAAGAG
                  ************************************************************

HsCD00514421      TTGGAGGAAGAGGAGGAGGAGGAGGAGTGGGAGCTCACCCAGAACGTGGTGGCCTTCGAT
NM_153270.1       TTGGAGGAAGAGGAGGAGGAGGAGGAGTGGGAGCTCACCCAGAACGTGGTGGCCTTCGAT
                  ************************************************************

HsCD00514421      GTGTACAATCACCGCTGGCGCAGCCTTACGCAGCTACCCACACCACTGCTGGGGCACAGC
NM_153270.1       GTGTACAATCACCGCTGGCGCAGCCTTACGCAGCTACCCACACCACTGCTGGGGCACAGC
                  ************************************************************

HsCD00514421      GTGTGCACCGCGGGCAACTTCCTGTTTGTCCTGGGTGGGGAGAGCCCTTCCGGCAGTGCA
NM_153270.1       GTGTGCACCGCGGGCAACTTCCTGTTTGTCCTGGGTGGGGAGAGCCCTTCCGGCAGTGCA
                  ************************************************************

HsCD00514421      TCCTCTCCCCTGGCCGACGACTCGCGGGTGGTCACGGCCCAAGTGCACCGTTACGACCCG
NM_153270.1       TCCTCTCCCCTGGCCGACGACTCGCGGGTGGTCACGGCCCAAGTTCACCGTTACGACCCG
                  ******************************************** ***************

HsCD00514421      CGCTTCCACGCTTGGACGGAAGTGCCCGCCATGCGGGAAGCGCGGGCCCACTTCTGGTGC
NM_153270.1       CGCTTCCACGCTTGGACGGAAGTGCCCGCCATGCGGGAAGCGCGGGCCCACTTCTGGTGC
                  ************************************************************

HsCD00514421      GGCGCGGTGGGCGAGAGGCTCCTGGCCGTCGGGGGCCTGGGTGCGGGCGGTGAGGTGCTG
NM_153270.1       GGCGCGGTGGGCGAGAGGCTCCTGGCCGTCGGGGGCCTGGGTGCGGGCGGTGAGGTGCTG
                  ************************************************************

HsCD00514421      GCCTCGGTGGAGATGTACGACCTGCGTCGGGACCGCTGGACGGCGGCTGGGGCACTACCG
NM_153270.1       GCCTCGGTGGAGATGTACGACCTGCGTCGGGACCGCTGGACGGCGGCTGGGGCACTACCG
                  ************************************************************

HsCD00514421      CGGGCTCTGCACGGTCACGCGGGGGCCGTCGGGGACCGCGGTGTTGTGTACATCTCGGGG
NM_153270.1       CGGGCTCTGCACGGTCACGCGGGGGCCGTCGGGGACCGCGGTGTTGTGTACATCTCGGGG
                  ************************************************************

HsCD00514421      GGCAAGGCAGGGAGAGGCGAGGGCGGAGCGAGCAGCCTCCGGGACTTATACGTCCTGGGC
NM_153270.1       GGCAAGGCAGGGAGAGGCGAGGGCGGAGCGAGCAGCCTCCGGGACTTATACGTCCTGGGC
                  ************************************************************

HsCD00514421      CCTGAGGAGCAGGTTTGGAGCAAGAAGGCACCCATGGGCACCGCACGTTTCGGGCACCAC
NM_153270.1       CCTGAGGAGCAGGTTTGGAGCAAGAAGGCACCCATGGGCACCGCACGTTTCGGGCACCAC
                  ************************************************************

HsCD00514421      ATGGCAGTGCTGCGCGGCGCTGTGTTTGCTTTTCTGGGGCGATACGAGCCCTTCTCTGAG
NM_153270.1       ATGGCAGTGCTGCGCGGCGCTGTGTTTGCTTTTCTGGGGCGATACGAGCCCTTCTCTGAG
                  ************************************************************

HsCD00514421      ATCGAGCGCTACGACCCCGGCGCCGACCAGTGGACTCGGTTGCGGCCGCTGCCCTACGAC
NM_153270.1       ATCGAGCGCTACGACCCCGGCGCCGACCAGTGGACTCGGTTGCGGCCGCTGCCCTACGAC
                  ************************************************************

HsCD00514421      CGCTTCTGCTATGGGCTGGCCGTGGTCGAGGAGACAGCGTTGCTGCTGGGCGGCCTCAAG
NM_153270.1       CGCTTCTGCTATGGGCTGGCCGTGGTCGAGGAGACAGCGTTGCTGCTGGGCGGCCTCAAG
                  ************************************************************

HsCD00514421      TGGCGGGACTCGCGCCAGGTGCCTACCCGCAACGTAGTGGGCTACGACCTCGACCTGGAC
NM_153270.1       TGGCGGGACTCGCGCCAGGTGCCTACCCGCAACGTAGTGGGCTACGACCTCGACCTGGAC
                  ************************************************************

HsCD00514421      CGTTGGGAGGACATCGGCTGCGCGTTGCCCTGGGCCTGGAGCGGCCTGCGGTGCGCAGTG
NM_153270.1       CGTTGGGAGGACATCGGCTGCGCGTTGCCCTGGGCCTGGAGCGGCCTGCGGTGCGCAGTG
                  ************************************************************

HsCD00514421      CTGCAGCTGGCCGAGGGTGGGGACGACGAGAGGGAGGGAGAGGTTGGAGAGGCGCTAGAT
NM_153270.1       CTGCAGCTGGCCGAGGGTGGGGACGACGAGAGGGAGGGAGAGGTTGGAGAGGCGCTAGAT
                  ************************************************************

HsCD00514421      TTGGTGCTGGGC---
NM_153270.1       TTGGTGCTGGGCTGA
                  ************