Gene:
Gene Symbol:
DBF4
Gene Name:
DBF4 homolog (S. cerevisiae)
Original Clone ID:
ccsbBroadEn_02563
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC036045.1,BC047693.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00514490
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAACTCCGGAGCCATGAGGATCCACAGTAAAGGACATTTCCAGGGTGGAATCCAAGTC
AAAAATGAAAAAAACAGACCATCTCTGAAATCTCTGAAAACTGATAACAGGCCAGAAAAA
TCCAAATGTAAGCCACTTTGGGGAAAAGTATTTTACCTTGACTTACCTTCTGTCACCATA
TCTGAAAAACTTCAAAAGGACATTAAGGATCTGGGAGGGCGAGTTGAAGAATTTCTCAGC
AAAGATATCAGTTATCTTATTTCAAATAAGAAGGAAGCTAAATTTGCACAAACCTTGGGT
CGAATTTCTCCTGTACCAAGTCCAGAATCTGCATATACTGCAGAAACCACTTCACCTCAT
CCCAGCCATGATGGAAGTTCATTTAAGTCACCAGACACAGTGTGTTTAAGCAGAGGAAAA
TTATTAGTTGAAAAAGCTATCAAGGACCATGATTTTATTCCTTCAAATAGTATATTATCA
AATGCCTTGTCATGGGGAGTAAAAATTCTTCATATTGATGACATTAGATACTACATTGAA
CAAAAGAAAAAAGAGTTGTATTTACTCAAGAAATCAAGTACTTCAGTAAGAGATGGGGGC
AAAAGAGTTGGTAGTGGTGCACAAAAAACAAGAACAGGAAGACTCAAAAAGCCTTTTGTA
AAGGTGGAAGATATGAGCCAACTTTATAGGCCATTTTATCTTCAGCTGACCAATATGCCT
TTTATAAATTATTCTATTCAGAAGCCCTGCAGTCCATTTGATGTAGACAAGCCATCTAGT
ATGCAAAAGCAAACTCAGGTTAAACTAAGAATCCAAACAGATGGCGATAAGTATGGTGGA
ACCTCAATTCAACTCCAGTTGAAAGAGAAGAAGAAAAAAGGATATTGTGAATGTTGCTTG
CAGAAATATGAAGATCTAGAAACTCACCTTCTAAGTGAGCAACACAGAAACTTTGCACAG
AGTAACCAGTATCAAGTTGTTGATGATATTGTATCTAAGTTAGTTTTTGACTTTGTGGAA
TATGAAAAGGACACACCTAAAAAGAAAAGAATAAAATACAGTGTTGGATCCCTTTCTCCT
GTTTCTGCAAGTGTCCTGAAAAAGACTGAACAAAAGGAAAAAGTGGAATTGCAACATATT
TCTCAGAAAGATTGCCAGGAAGATGATACAACAGTGAAGGAGCAGAATTTCCTGTATAAA
GAGACCCAGGAAACTGAAAAAAAGCTCCTGTTTATTTCAGAGCCCATCCCCCACCCTTCA
AATGAATTGAGAGGGCTTAATGAGAAAATGAGTAATAAATGTTCCATGTTAAGTACAGCT
GAAGATGACATAAGACAGAATTTTACACAGCTACCTCTACATAAAAACAAACAGGAATGC
ATTCTTGACATTTCCGAACACACATTAAGTGAAAATGACTTAGAAGAACTAAGGGTAGAT
CACTATAAATGTAACATACAGGCATCTGTACATGTTTCTGATTTCAGTACAGATAATAGT
GGATCTCAACCAAAACAGAAGTCAGATACTGTGCTTTTTCCAGCAAAGGATCTCAAGGAA
AAGGACCTTCATTCAATATTTACTCATGATTCTGGTCTGATAACAATAAACAGTTCACAA
GAGCACCTAACTGTTCAGGCAAAGGCTCCATTCCATACTCCTCCTGAGGAACCCAATGAA
TGTGACTTCAAGAATATGGATAGTTTACCTTCTGGTAAAATACATCGAAAAGTGAAAATA
ATATTAGGACGAAATAGAAAAGAAAATCTGGAACCAAATGCTGAATTTGATAAAAGAACT
GAATTTATTACACAAGAAGAAAACAGAATTTGTAGTTCACCGGTACAGTCTTTACTAGAC
TTGTTTCAGACTAGTGAAGAGAAATCAGAATTTTTGGGTTTCACAAGCTACACAGAAAAG
AGTGGTATATGCAATGTTTTAGATATTTGGGAAGAGGAAAATTCAGATAATCTGTTAACA
GCGTTTTTCTCGTCCCCTTCAACTTCTACATTTACTGGCTTT
Protein sequence predicted based on nucleotide sequence
MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTI SEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLGRISPVPSPESAYTAETTSPH PSHDGSSFKSPDTVCLSRGKLLVEKAIKDHDFIPSNSILSNALSWGVKILHIDDIRYYIE QKKKELYLLKKSSTSVRDGGKRVGSGAQKTRTGRLKKPFVKVEDMSQLYRPFYLQLTNMP FINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCL QKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSP VSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPS NELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVD HYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQ EHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRT EFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLT AFFSSPSTSTFTGF
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
