Gene:
Gene Symbol:
ADD3
Gene Name:
adducin 3 (gamma)
Original Clone ID:
ccsbBroadEn_00026
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC062559.1
Comments:
tube source did not grow. Plate copy did not cocntig with ref seq. BLAST results returned HsCD00622995 (81137-C05)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00514541
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAGCTCAGATGCCAGCCAAGGCGTGATTACCACTCCTCCTCCTCCCAGCATGCCTCAC
AAAGAGAGATATTTTGACCGCATCAATGAAAATGACCCAGAATACATTAGGGAGAGGAAC
ATGTCTCCTGATCTACGACAAGACTTCAACATGATGGAGCAGAGGAAACGAGTTACTCAG
ATCCTGCAAAGTCCTGCCTTTCGGGAAGACTTGGAATGCCTTATTCAAGAACAGATGAAG
AAAGGCCACAACCCAACTGGATTACTAGCATTACAGCAGATTGCAGATTACATCATGGCC
AATTCTTTCTCGGGTTTTTCTTCACCTCCTCTCAGTCTTGGCATGGTCACACCTATCAAT
GACCTTCCTGGTGCAGATACATCCTCATATGTGAAGGGAGAAAAACTTACTCGCTGTAAA
CTTGCCAGCCTGTACAGACTTGTAGACTTGTTTGGATGGGCACACCTGGCAAATACCTAT
ATCTCAGTAAGAATAAGTAAGGAGCAAGACCACATTATAATAATTCCCAGAGGCCTATCT
TTTTCTGAAGCTACAGCCTCCAATTTGGTGAAAGTCAATATAATAGGAGAAGTGGTTGAC
CAGGGAAGTACCAATTTGAAAATTGACCATACAGGATTCAGTCCCCATGCTGCAATCTAT
TCAACACGTCCTGATGTTAAGTGTGTGATACACATCCATACCCTTGCAACAGCAGCTGTA
TCCTCCATGAAATGTGGGATCCTTCCAATTTCTCAAGAGTCTCTTCTTCTGGGAGATGTT
GCCTATTATGACTACCAAGGGTCACTTGAAGAACAGGAGGAGAGAATTCAACTGCAGAAG
GTTCTGGGACCAAGTTGTAAGGTGCTGGTACTCAGGAATCATGGTGTGGTTGCACTTGGA
GAAACATTAGAGGAGGCTTTTCATTATATTTTTAATGTGCAACTAGCCTGTGAGATTCAG
GTGCAGGCCCTAGCAGGTGCAGGTGGAGTAGACAATCTCCATGTACTGGACTTTCAGAAG
TATAAAGCTTTCACTTACACTGTAGCAGCGTCTGGTGGAGGAGGTGTGAATATGGGTTCC
CATCAAAAATGGAAGGTTGGCGAAATTGAGTTTGAAGGGCTTATGAGGACTCTGGACAAC
TTGGGGTATAGAACAGGCTATGCTTACAGGCATCCTCTCATTCGAGAGAAGCCTAGGCAC
AAGAGTGATGTGGAAATCCCAGCAACTGTGACTGCTTTTTCCTTTGAAGACGATACAGTG
CCACTCTCTCCTCTCAAATACATGGCACAGAGGCAACAGCGTGAAAAAACAAGATGGCTG
AACTCACCAAATACTTACATGAAAGTGAATGTGCCTGAGGAGTCTCGGAACGGAGAAACC
AGTCCCCGAACCAAAATCACGTGGATGAAAGCAGAAGACTCATCTAAAGTTAGTGGTGGA
ACACCTATCAAAATTGAAGATCCAAATCAGTTTGTTCCTTTAAACACAAACCCGAATGAG
GTACTAGAAAAGAGAAATAAGATTCGGGAACAAAATCGATATGACTTGAAAACAGCAGGA
CCACAATCTCAGTTGCTTGCTGGAATTGTTGTGGATAAGCCACCTTCTACTATGCAATTT
GAAGATGATGATCATGGCCCACCAGCTCCTCCTAACCCATTTAGTCATCTCACAGAAGGA
GAACTTGAAGAGTATAAGAGGACAATCGAACGTAAACAACAAGGCCTAGAAGATGCTGAG
CAGGAATTACTCTCAGATGACGCTTCATCTGTTTCACAAATTCAGTCTCAAACTCAGTCA
CCGCAAAATGTCCCTGAAAAATTAGAAGAAAACCATGAGCTGTTTTCCAAGAGCTTCATC
TCCATGGAAGTGCCTGTCATGGTAGTAAATGGCAAGGATGATATGCATGATGTTGAAGAT
GAGCTTGCTAAGCGAGTGAGTAGGTTAAGCACAAGTACAACCATAGAAAACATCGAGATT
ACTATTAAGTCTCCAGAGAAAATCGAAGAAGTCCTGTCACCTGAAGGCTCCCCTTCAAAA
TCGCCATCCAAGAAAAAGAAGAAATTCCGCACTCCTTCTTTTCTGAAAAAGAACAAAAAA
AAGGAGAAAGTTGAGGCC
Protein sequence predicted based on nucleotide sequence
MSSDASQGVITTPPPPSMPHKERYFDRINENDPEYIRERNMSPDLRQDFNMMEQRKRVTQ ILQSPAFREDLECLIQEQMKKGHNPTGLLALQQIADYIMANSFSGFSSPPLSLGMVTPIN DLPGADTSSYVKGEKLTRCKLASLYRLVDLFGWAHLANTYISVRISKEQDHIIIIPRGLS FSEATASNLVKVNIIGEVVDQGSTNLKIDHTGFSPHAAIYSTRPDVKCVIHIHTLATAAV SSMKCGILPISQESLLLGDVAYYDYQGSLEEQEERIQLQKVLGPSCKVLVLRNHGVVALG ETLEEAFHYIFNVQLACEIQVQALAGAGGVDNLHVLDFQKYKAFTYTVAASGGGGVNMGS HQKWKVGEIEFEGLMRTLDNLGYRTGYAYRHPLIREKPRHKSDVEIPATVTAFSFEDDTV PLSPLKYMAQRQQREKTRWLNSPNTYMKVNVPEESRNGETSPRTKITWMKAEDSSKVSGG TPIKIEDPNQFVPLNTNPNEVLEKRNKIREQNRYDLKTAGPQSQLLAGIVVDKPPSTMQF EDDDHGPPAPPNPFSHLTEGELEEYKRTIERKQQGLEDAEQELLSDDASSVSQIQSQTQS PQNVPEKLEENHELFSKSFISMEVPVMVVNGKDDMHDVEDELAKRVSRLSTSTTIENIEI TIKSPEKIEEVLSPEGSPSKSPSKKKKKFRTPSFLKKNKKKEKVEA
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
