ASAP3 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  ASAP3
Gene Name:  ArfGAP with SH3 domain, ankyrin repeat and PH domain 3
Original Clone ID:
ccsbBroadEn_08552
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC060786.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00514636
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2710nts
Open reading frame: 1 to 2710

ATGCCGGAGCAGTTCAGCGTCGCCGAGTTCCTGGCCGTCACCGCGGAGGACCTCAGCTCC
CCGGCTGGGGCCGCCGCCTTCGCCGCCAAGATGCCCCGGTACCGAGGGGCGGCGCTGGCG
CGGGAGGAGATCTTGGAAGGAGACCAAGCCATCCTGCAGAGAATAAAGAAGGCTGTGCGG
GCAATCCATAGCTCCGGCCTTGGCCATGTGGAGAATGAAGAGCAGTACCGAGAGGCCGTG
GAATCCTTAGGCAACAGCCACCTGTCCCAGAACAGCCATGAGCTGTCCACAGGCTTCCTA
AACTTGGCCGTGTTCACCCGCGAGGTTGCTGCGCTCTTCAAGAACCTGATTCAGAACTTG
AACAACATTGTCTCTTTCCCCCTGGACAGTCTGATGAAGGGGCAGCTGAGGGACGGTCGA
CAGGATTCCAAAAAACAGCTGGAGAAGGCATGGAAGGACTATGAAGCCAAAATGGCCAAG
CTGGAGAAGGAGCGCGATCGGGCCAGGGTGACAGGAGGGATCCCTGGGGAGGTGGCCCAG
GACATGCAGAGAGAGCGGCGCATCTTCCAGCTGCACATGTGTGAGTATCTGCTCAAAGCC
GGGGAGAGCCAGATGAAGCAAGGTCCTGACTTCCTTCAGAGCCTCATCAAGTTCTTCCAC
GCCCAGCACAACTTTTTCCAAGATGGCTGGAAGGCTGCCCAGAGCCTGTTCCCCTTCATC
GAGAAGCTGGCGGCCTCAGTACATGCACTCCATCAGGCCCAGGAGGACGAACTACAGAAG
CTGACCCAGCTCCGGGACTCCCTCCGAGGGACACTGCAGCTTGAGAGCAGAGAGGAACAC
CTGAGCCGGAAGAACTCAGGATGTGGCTATAGCATCCACCAGCACCAAGGCAACAAGCAG
TTTGGGACGGAGAAAGTGGGCTTTCTATACAAGAAAAGTGACGGAATTCGAAGAGCCTGG
CAGAAAAGGAAGTGTGGAGTCAAGTATGGCTGCCTGACCATCTCACACAGCACGATAAAC
CGGCCCCCGGTGAAGCTGACCCTGCTGACGTGCCAAGTGAGGCCAAACCCTGAGGAGAAA
AAGTGCTTCGACCTGGTGACCCACAACCGGACGTACCACTTTCAGGCAGAGGACGAGCAC
GAGTGTGAGGCGTGGGTGTCAGTGTTGCAGAACAGCAAGGACGAAGCCCTGAGCAGCGCC
TTCCTCGGGGAGCCCAGCGCTGGCCCGGGGTCCTGGGGGTCCGCCGGCCATGATGGGGAG
CCGCACGACCTCACAAAGCTGCTCATCGCGGAGGTGAAGAGCAGGCCTGGGAATAGCCAG
TGCTGCGACTGCGGGGCTGCAGACCCCACGTGGCTCAGCACCAACCTGGGCGTGCTCACC
TGCATCCAGTGCTCGGGCGTCCACCGCGAACTGGGCGTGCGCTTTTCGCGCATGCAGTCA
CTCACCTTGGACCTGCTGGGCCCCTCCGAGTTGTTGCTGGCCTTGAACATGGGAAACACG
AGCTTCAATGAGGTCATGGAGGCCCAGCTACCCTCACACGGCGGCCCTAAACCCTCAGCT
GAGAGTGACATGGGCACCCGCAGGGACTACATTATGGCCAAGTATGTGGAGCATAGGTTT
GCACGCCGGTGCACACCTGAGCCTCAGCGACTCTGGACAGCCATTTGCAACAGGGACCTC
CTGTCGGTACTGGAGGCCTTTGCCAATGGGCAGGACTTTGGACAGCCGCTGCCAGGGCCT
GATGCACAGGCACCTGAAGAACTCGTCTTGCATTTGGCTGTCAAAGTCGCCAACCAGGCT
TCCCTGCCTCTGGTGGATTTCATCATCCAGAACGGTGGTCACCTGGATGCCAAGGCTGCT
GACGGGAACACGGCTCTGCACTACGCAGCACTCTACAACCAGCCCGACTGCCTCAAGCTG
CTGCTGAAGGGGAGAGCTTTGGTTGGCACAGTAAATGAAGCAGGCGAGACAGCTCTGGAC
ATAGCCAGGAAGAAGCACCACAAGGAGTGTGAGGAGCTGCTGGAGCAGGCCCAGGCGGGG
ACCTTTGCCTTCCCTCTACATGTGGACTACTCCTGGGTAATTTCCACAGAGCCTGGCTCT
GACAGTGAGGAGGATGAGGAAGAGAAGCGCTGCTTGCTGAAGCTCCCGGCCCAGGCTCAC
TGGGCCAGTGGGAGGCTGGACATCAGCAACAAGACCTATGAGACTGTCGCCAGCCTGGGA
GCAGCCACCCCTCAGGGCGAGAGTGAGGACTGTCCCCCGCCCTTGCCAGTCAAAAACTCT
TCTCGGACTTTGGTCCAAGGGTGTGCAAGACATGCCAGTGGAGATCGTTCTGAAGTCTCC
AGCCTGAGTTCAGAGGCCCCTGAGACCCCTGAGAGCCTGGGCAGTCCAGCCTCCTCCTCC
AGTCTGATGAGCCCCTTGGAACCTGGGGATCCCAGCCAAGCCCCACCCAACTCTGAAGAG
GGCCTCCGAGAGCCCCCAGGCACCTCCAGACCCAGCCTGACATCCGGGACCACCCCTTCG
GAGATGTACCTCCCCGTCAGATTCAGCTCCGAGAGCACTCGCTCCTATCGGCGGGGGGCG
CGGAGCCCTGAAGATGGTCCCTCAGCCAGGCAGCCTCTGCCCAGAAGGAACGTGCCGGTT
GGCATCACTGAAGGAGATGGCTCAAGGACTGGGAGTCTCCCAGCAAGTTCTGTGCAACTT
TTGCAAGAC

Protein sequence predicted based on nucleotide sequence

MPEQFSVAEFLAVTAEDLSSPAGAAAFAAKMPRYRGAALAREEILEGDQAILQRIKKAVR
AIHSSGLGHVENEEQYREAVESLGNSHLSQNSHELSTGFLNLAVFTREVAALFKNLIQNL
NNIVSFPLDSLMKGQLRDGRQDSKKQLEKAWKDYEAKMAKLEKERDRARVTGGIPGEVAQ
DMQRERRIFQLHMCEYLLKAGESQMKQGPDFLQSLIKFFHAQHNFFQDGWKAAQSLFPFI
EKLAASVHALHQAQEDELQKLTQLRDSLRGTLQLESREEHLSRKNSGCGYSIHQHQGNKQ
FGTEKVGFLYKKSDGIRRAWQKRKCGVKYGCLTISHSTINRPPVKLTLLTCQVRPNPEEK
KCFDLVTHNRTYHFQAEDEHECEAWVSVLQNSKDEALSSAFLGEPSAGPGSWGSAGHDGE
PHDLTKLLIAEVKSRPGNSQCCDCGAADPTWLSTNLGVLTCIQCSGVHRELGVRFSRMQS
LTLDLLGPSELLLALNMGNTSFNEVMEAQLPSHGGPKPSAESDMGTRRDYIMAKYVEHRF
ARRCTPEPQRLWTAICNRDLLSVLEAFANGQDFGQPLPGPDAQAPEELVLHLAVKVANQA
SLPLVDFIIQNGGHLDAKAADGNTALHYAALYNQPDCLKLLLKGRALVGTVNEAGETALD
IARKKHHKECEELLEQAQAGTFAFPLHVDYSWVISTEPGSDSEEDEEEKRCLLKLPAQAH
WASGRLDISNKTYETVASLGAATPQGESEDCPPPLPVKNSSRTLVQGCARHASGDRSEVS
SLSSEAPETPESLGSPASSSSLMSPLEPGDPSQAPPNSEEGLREPPGTSRPSLTSGTTPS
EMYLPVRFSSESTRSYRRGARSPEDGPSARQPLPRRNVPVGITEGDGSRTGSLPASSVQL
LQD

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable